CatLogenzyme kinetics catalog

EC 2.7.8.13: phospho-N-acetylmuramoyl-pentapeptide-transferase

TransferasesOpen in Browse

CatLog read 47 records for EC 2.7.8.13 from 2 papers, for 2 organisms. Names in these papers: MraY (phospho-N-acetylmuramoyl-pentapeptide transferase), phospho-N-acetylmuramyl-pentapeptide translocase.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units.

CatLog also holds 7 records for EC 2.7.8.13 that are not yet re-read: from its earlier version, built from the enzyme databases and partly checked against the papers. All records for EC 2.7.8.13 in Browse.

Papers

PaperOrganismsRecords
Active site mapping of MraY, a member of the polyprenyl-phosphate N-acetylhexosamine 1-phosphate transferase superfamily, catalyzing the first membrane step of peptidoglycan biosynthesisBacillus subtilis30
On the specificity of phospho-N-acetylmuramyl-pentapeptide translocase. The peptide subunit of uridine diphosphate-N-actylmuramyl-pentapeptideStaphylococcus aureus17

Records

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)ConditionsPaper
MraY (phospho-N-acetylmuramoyl-pentapeptide transferase)Bacillus subtilisUDP-N-acetylmuramoyl-L-Ala-D-iGlu-meso-diaminopimeloyl-D-Ala-D-Ala5.330.945.7 calc.pH 7.6PMID 18672909Open
MraY (phospho-N-acetylmuramoyl-pentapeptide transferase)Bacillus subtilisundecaprenyl phosphate—0.16—pH 7.6PMID 18672909Open
MraY (phospho-N-acetylmuramoyl-pentapeptide transferase)variant D174NBacillus subtilisUDP-N-acetylmuramoyl-L-Ala-D-iGlu-meso-diaminopimeloyl-D-Ala-D-Ala0.01230.990.012 calc.pH 7.6PMID 18672909Open
MraY (phospho-N-acetylmuramoyl-pentapeptide transferase)variant D174NBacillus subtilisundecaprenyl phosphate—0.17—pH 7.6PMID 18672909Open
MraY (phospho-N-acetylmuramoyl-pentapeptide transferase)variant D177NBacillus subtilisUDP-N-acetylmuramoyl-L-Ala-D-iGlu-meso-diaminopimeloyl-D-Ala-D-Ala0.0650.860.076 calc.pH 7.6PMID 18672909Open
MraY (phospho-N-acetylmuramoyl-pentapeptide transferase)variant D177NBacillus subtilisundecaprenyl phosphate—0.23—pH 7.6PMID 18672909Open
MraY (phospho-N-acetylmuramoyl-pentapeptide transferase)variant D231NBacillus subtilisUDP-N-acetylmuramoyl-L-Ala-D-iGlu-meso-diaminopimeloyl-D-Ala-D-Ala0.016310.016 calc.pH 7.6PMID 18672909Open
MraY (phospho-N-acetylmuramoyl-pentapeptide transferase)variant D231NBacillus subtilisundecaprenyl phosphate—0.17—pH 7.6PMID 18672909Open
MraY (phospho-N-acetylmuramoyl-pentapeptide transferase)variant D98NBacillus subtilisUDP-N-acetylmuramoyl-L-Ala-D-iGlu-meso-diaminopimeloyl-D-Ala-D-Ala0.00120.340.0034 calc.pH 7.6PMID 18672909Open
MraY (phospho-N-acetylmuramoyl-pentapeptide transferase)variant D98NBacillus subtilisundecaprenyl phosphate—0.32—pH 7.6PMID 18672909Open
MraY (phospho-N-acetylmuramoyl-pentapeptide transferase)variant D99NBacillus subtilisUDP-N-acetylmuramoyl-L-Ala-D-iGlu-meso-diaminopimeloyl-D-Ala-D-Ala8.3 × 10⁻⁴0.760.0011 calc.pH 7.6PMID 18672909Open
MraY (phospho-N-acetylmuramoyl-pentapeptide transferase)variant D99NBacillus subtilisundecaprenyl phosphate—0.35—pH 7.6PMID 18672909Open
MraY (phospho-N-acetylmuramoyl-pentapeptide transferase)variant E299QBacillus subtilisUDP-N-acetylmuramoyl-L-Ala-D-iGlu-meso-diaminopimeloyl-D-Ala-D-Ala0.531.40.38 calc.pH 7.6PMID 18672909Open
MraY (phospho-N-acetylmuramoyl-pentapeptide transferase)variant E299QBacillus subtilisundecaprenyl phosphate—0.22—pH 7.6PMID 18672909Open
MraY (phospho-N-acetylmuramoyl-pentapeptide transferase)variant H289RBacillus subtilisUDP-N-acetylmuramoyl-L-Ala-D-iGlu-meso-diaminopimeloyl-D-Ala-D-Ala5 × 10⁻⁵1.14.5 × 10⁻⁵ calc.pH 7.6PMID 18672909Open
MraY (phospho-N-acetylmuramoyl-pentapeptide transferase)variant H289RBacillus subtilisundecaprenyl phosphate—0.25—pH 7.6PMID 18672909Open
MraY (phospho-N-acetylmuramoyl-pentapeptide transferase)variant H290RBacillus subtilisUDP-N-acetylmuramoyl-L-Ala-D-iGlu-meso-diaminopimeloyl-D-Ala-D-Ala0.00381.30.0029 calc.pH 7.6PMID 18672909Open
MraY (phospho-N-acetylmuramoyl-pentapeptide transferase)variant H290RBacillus subtilisundecaprenyl phosphate—0.15—pH 7.6PMID 18672909Open
MraY (phospho-N-acetylmuramoyl-pentapeptide transferase)variant H291RBacillus subtilisUDP-N-acetylmuramoyl-L-Ala-D-iGlu-meso-diaminopimeloyl-D-Ala-D-Ala0.0323.40.0093 calc.pH 7.6PMID 18672909Open
MraY (phospho-N-acetylmuramoyl-pentapeptide transferase)variant H291RBacillus subtilisundecaprenyl phosphate—0.27—pH 7.6PMID 18672909Open
MraY (phospho-N-acetylmuramoyl-pentapeptide transferase)variant H45RBacillus subtilisUDP-N-acetylmuramoyl-L-Ala-D-iGlu-meso-diaminopimeloyl-D-Ala-D-Ala0.0233.40.0069 calc.pH 7.6PMID 18672909Open
MraY (phospho-N-acetylmuramoyl-pentapeptide transferase)variant H45RBacillus subtilisundecaprenyl phosphate—0.22—pH 7.6PMID 18672909Open
MraY (phospho-N-acetylmuramoyl-pentapeptide transferase)variant K116QBacillus subtilisUDP-N-acetylmuramoyl-L-Ala-D-iGlu-meso-diaminopimeloyl-D-Ala-D-Ala0.001527.5 × 10⁻⁴ calc.pH 7.6PMID 18672909Open
MraY (phospho-N-acetylmuramoyl-pentapeptide transferase)variant K116QBacillus subtilisundecaprenyl phosphate—0.22—pH 7.6PMID 18672909Open
MraY (phospho-N-acetylmuramoyl-pentapeptide transferase)variant K48QBacillus subtilisUDP-N-acetylmuramoyl-L-Ala-D-iGlu-meso-diaminopimeloyl-D-Ala-D-Ala0.0271.30.021 calc.pH 7.6PMID 18672909Open
MraY (phospho-N-acetylmuramoyl-pentapeptide transferase)variant K48QBacillus subtilisundecaprenyl phosphate—0.26—pH 7.6PMID 18672909Open
MraY (phospho-N-acetylmuramoyl-pentapeptide transferase)variant N168ABacillus subtilisUDP-N-acetylmuramoyl-L-Ala-D-iGlu-meso-diaminopimeloyl-D-Ala-D-Ala0.0720.950.075 calc.pH 7.6PMID 18672909Open
MraY (phospho-N-acetylmuramoyl-pentapeptide transferase)variant N168ABacillus subtilisundecaprenyl phosphate—0.21—pH 7.6PMID 18672909Open
MraY (phospho-N-acetylmuramoyl-pentapeptide transferase)variant N171ABacillus subtilisUDP-N-acetylmuramoyl-L-Ala-D-iGlu-meso-diaminopimeloyl-D-Ala-D-Ala0.01350.730.018 calc.pH 7.6PMID 18672909Open
MraY (phospho-N-acetylmuramoyl-pentapeptide transferase)variant N171ABacillus subtilisundecaprenyl phosphate—0.24—pH 7.6PMID 18672909Open
phospho-N-acetylmuramyl-pentapeptide translocaseStaphylococcus aureusUDP-N-acetylmuramoyl-glycyl-gamma-D-glutamyl-L-lysyl-D-alanyl-D-alanine0.16 unit bacterial alkaline phosphatase; 60 µl—0.5—pH 7.8, 25 °CPMID 4208473Open
phospho-N-acetylmuramyl-pentapeptide translocaseStaphylococcus aureusUDP-N-acetylmuramoyl-glycyl-gamma-D-glutamyl-L-lysyl-D-alanyl-D-alanine3.3 x 10^-5 M [3H]UMP; 16 min at 25 °C; [3H]UMP—0.044—pH 7.8, 25 °CPMID 4208473Open
phospho-N-acetylmuramyl-pentapeptide translocaseStaphylococcus aureusUDP-N-acetylmuramoyl-glycyl-gamma-D-glutamyl-L-lysyl-D-alanyl-glycine—0.08—pH 7.8, 25 °CPMID 4208473Open
phospho-N-acetylmuramyl-pentapeptide translocaseStaphylococcus aureusUDP-N-acetylmuramoyl-glycyl-gamma-D-glutamyl-L-lysyl-glycyl-D-alanine—0.27—pH 7.8, 25 °CPMID 4208473Open
phospho-N-acetylmuramyl-pentapeptide translocaseStaphylococcus aureusUDP-N-acetylmuramoyl-L-alanyl-gamma-D-glutamyl-L-lysine0.16 unit bacterial alkaline phosphatase; 60 µl—4.4—pH 7.8, 25 °CPMID 4208473Open
phospho-N-acetylmuramyl-pentapeptide translocaseStaphylococcus aureusUDP-N-acetylmuramoyl-L-alanyl-gamma-D-glutamyl-L-lysine3.3 x 10^-5 M [3H]UMP; 16 min at 25 °C; [3H]UMP—0.18—pH 7.8, 25 °CPMID 4208473Open
phospho-N-acetylmuramyl-pentapeptide translocaseStaphylococcus aureusUDP-N-acetylmuramoyl-L-alanyl-gamma-D-glutamyl-L-lysyl-D-alanine0.16 unit bacterial alkaline phosphatase; 60 µl—0.4—pH 7.8, 25 °CPMID 4208473Open
phospho-N-acetylmuramyl-pentapeptide translocaseStaphylococcus aureusUDP-N-acetylmuramoyl-L-alanyl-gamma-D-glutamyl-L-lysyl-D-alanine3.3 x 10^-5 M [3H]UMP; 16 min at 25 °C; [3H]UMP—0.058—pH 7.8, 25 °CPMID 4208473Open
phospho-N-acetylmuramyl-pentapeptide translocaseStaphylococcus aureusUDP-N-acetylmuramoyl-L-alanyl-gamma-D-glutamyl-L-lysyl-D-alanyl-D-alanine0.16 unit bacterial alkaline phosphatase; 60 µl—0.055—pH 7.8, 25 °CPMID 4208473Open
phospho-N-acetylmuramyl-pentapeptide translocaseStaphylococcus aureusUDP-N-acetylmuramoyl-L-alanyl-gamma-D-glutamyl-L-lysyl-D-alanyl-D-alanine3.3 x 10^-5 M [3H]UMP; 16 min at 25 °C; [3H]UMP—0.02—pH 7.8, 25 °CPMID 4208473Open
phospho-N-acetylmuramyl-pentapeptide translocaseStaphylococcus aureusUDP-N-acetylmuramoyl-L-alanyl-gamma-D-glutamyl-L-lysyl-D-alanyl-glycine0.16 unit bacterial alkaline phosphatase; 60 µl—0.083—pH 7.8, 25 °CPMID 4208473Open
phospho-N-acetylmuramyl-pentapeptide translocaseStaphylococcus aureusUDP-N-acetylmuramoyl-L-alanyl-gamma-D-glutamyl-L-lysyl-D-alanyl-glycine3.3 x 10^-5 M [3H]UMP; 16 min at 25 °C; [3H]UMP—0.029—pH 7.8, 25 °CPMID 4208473Open
phospho-N-acetylmuramyl-pentapeptide translocaseStaphylococcus aureusUDP-N-acetylmuramoyl-L-alanyl-gamma-D-glutamyl-L-lysyl-glycyl-D-alanine0.16 unit bacterial alkaline phosphatase; 60 µl—0.44—pH 7.8, 25 °CPMID 4208473Open
phospho-N-acetylmuramyl-pentapeptide translocaseStaphylococcus aureusUDP-N-acetylmuramoyl-L-alanyl-gamma-D-glutamyl-L-lysyl-glycyl-D-alanine3.3 x 10^-5 M [3H]UMP; 16 min at 25 °C; [3H]UMP—0.063—pH 7.8, 25 °CPMID 4208473Open
phospho-N-acetylmuramyl-pentapeptide translocaseStaphylococcus aureusUDP-N-acetylmuramoyl-L-alanyl-gamma-D-glutamyl-L-ornithyl-D-alanyl-D-alanine—0.016—pH 7.8, 25 °CPMID 4208473Open
phospho-N-acetylmuramyl-pentapeptide translocaseStaphylococcus aureusUDP-N-acetylmuramoyl-L-alanyl-gamma-D-glutamyl-meso-2,6-diaminopimeloyl-D-alanyl-D-alanine0.16 unit bacterial alkaline phosphatase; 60 µl—0.1—pH 7.8, 25 °CPMID 4208473Open
phospho-N-acetylmuramyl-pentapeptide translocaseStaphylococcus aureusUDP-N-acetylmuramoyl-L-alanyl-gamma-D-glutamyl-meso-2,6-diaminopimeloyl-D-alanyl-D-alanine3.3 x 10^-5 M [3H]UMP; 16 min at 25 °C; [3H]UMP—0.022—pH 7.8, 25 °CPMID 4208473Open