CatLogenzyme kinetics catalog

Values CatLog read from this paper

Active site mapping of MraY, a member of the polyprenyl-phosphate N-acetylhexosamine 1-phosphate transferase superfamily, catalyzing the first membrane step of peptidoglycan biosynthesis

PMID 18672909doi:10.1021/bi8006274Open in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 30 records (45 values) from this paper: MraY (phospho-N-acetylmuramoyl-pentapeptide transferase) from Bacillus subtilis.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 15 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.

EC 2.7.8.13

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)Conditions
MraY (phospho-N-acetylmuramoyl-pentapeptide transferase)Bacillus subtilisUDP-N-acetylmuramoyl-L-Ala-D-iGlu-meso-diaminopimeloyl-D-Ala-D-Ala5.330.945.7 calc.pH 7.6Open
MraY (phospho-N-acetylmuramoyl-pentapeptide transferase)Bacillus subtilisundecaprenyl phosphate—0.16—pH 7.6Open
MraY (phospho-N-acetylmuramoyl-pentapeptide transferase)variant D174NBacillus subtilisUDP-N-acetylmuramoyl-L-Ala-D-iGlu-meso-diaminopimeloyl-D-Ala-D-Ala0.01230.990.012 calc.pH 7.6Open
MraY (phospho-N-acetylmuramoyl-pentapeptide transferase)variant D174NBacillus subtilisundecaprenyl phosphate—0.17—pH 7.6Open
MraY (phospho-N-acetylmuramoyl-pentapeptide transferase)variant D177NBacillus subtilisUDP-N-acetylmuramoyl-L-Ala-D-iGlu-meso-diaminopimeloyl-D-Ala-D-Ala0.0650.860.076 calc.pH 7.6Open
MraY (phospho-N-acetylmuramoyl-pentapeptide transferase)variant D177NBacillus subtilisundecaprenyl phosphate—0.23—pH 7.6Open
MraY (phospho-N-acetylmuramoyl-pentapeptide transferase)variant D231NBacillus subtilisUDP-N-acetylmuramoyl-L-Ala-D-iGlu-meso-diaminopimeloyl-D-Ala-D-Ala0.016310.016 calc.pH 7.6Open
MraY (phospho-N-acetylmuramoyl-pentapeptide transferase)variant D231NBacillus subtilisundecaprenyl phosphate—0.17—pH 7.6Open
MraY (phospho-N-acetylmuramoyl-pentapeptide transferase)variant D98NBacillus subtilisUDP-N-acetylmuramoyl-L-Ala-D-iGlu-meso-diaminopimeloyl-D-Ala-D-Ala0.00120.340.0034 calc.pH 7.6Open
MraY (phospho-N-acetylmuramoyl-pentapeptide transferase)variant D98NBacillus subtilisundecaprenyl phosphate—0.32—pH 7.6Open
MraY (phospho-N-acetylmuramoyl-pentapeptide transferase)variant D99NBacillus subtilisUDP-N-acetylmuramoyl-L-Ala-D-iGlu-meso-diaminopimeloyl-D-Ala-D-Ala8.3 × 10⁻⁴0.760.0011 calc.pH 7.6Open
MraY (phospho-N-acetylmuramoyl-pentapeptide transferase)variant D99NBacillus subtilisundecaprenyl phosphate—0.35—pH 7.6Open
MraY (phospho-N-acetylmuramoyl-pentapeptide transferase)variant E299QBacillus subtilisUDP-N-acetylmuramoyl-L-Ala-D-iGlu-meso-diaminopimeloyl-D-Ala-D-Ala0.531.40.38 calc.pH 7.6Open
MraY (phospho-N-acetylmuramoyl-pentapeptide transferase)variant E299QBacillus subtilisundecaprenyl phosphate—0.22—pH 7.6Open
MraY (phospho-N-acetylmuramoyl-pentapeptide transferase)variant H289RBacillus subtilisUDP-N-acetylmuramoyl-L-Ala-D-iGlu-meso-diaminopimeloyl-D-Ala-D-Ala5 × 10⁻⁵1.14.5 × 10⁻⁵ calc.pH 7.6Open
MraY (phospho-N-acetylmuramoyl-pentapeptide transferase)variant H289RBacillus subtilisundecaprenyl phosphate—0.25—pH 7.6Open
MraY (phospho-N-acetylmuramoyl-pentapeptide transferase)variant H290RBacillus subtilisUDP-N-acetylmuramoyl-L-Ala-D-iGlu-meso-diaminopimeloyl-D-Ala-D-Ala0.00381.30.0029 calc.pH 7.6Open
MraY (phospho-N-acetylmuramoyl-pentapeptide transferase)variant H290RBacillus subtilisundecaprenyl phosphate—0.15—pH 7.6Open
MraY (phospho-N-acetylmuramoyl-pentapeptide transferase)variant H291RBacillus subtilisUDP-N-acetylmuramoyl-L-Ala-D-iGlu-meso-diaminopimeloyl-D-Ala-D-Ala0.0323.40.0093 calc.pH 7.6Open
MraY (phospho-N-acetylmuramoyl-pentapeptide transferase)variant H291RBacillus subtilisundecaprenyl phosphate—0.27—pH 7.6Open
MraY (phospho-N-acetylmuramoyl-pentapeptide transferase)variant H45RBacillus subtilisUDP-N-acetylmuramoyl-L-Ala-D-iGlu-meso-diaminopimeloyl-D-Ala-D-Ala0.0233.40.0069 calc.pH 7.6Open
MraY (phospho-N-acetylmuramoyl-pentapeptide transferase)variant H45RBacillus subtilisundecaprenyl phosphate—0.22—pH 7.6Open
MraY (phospho-N-acetylmuramoyl-pentapeptide transferase)variant K116QBacillus subtilisUDP-N-acetylmuramoyl-L-Ala-D-iGlu-meso-diaminopimeloyl-D-Ala-D-Ala0.001527.5 × 10⁻⁴ calc.pH 7.6Open
MraY (phospho-N-acetylmuramoyl-pentapeptide transferase)variant K116QBacillus subtilisundecaprenyl phosphate—0.22—pH 7.6Open
MraY (phospho-N-acetylmuramoyl-pentapeptide transferase)variant K48QBacillus subtilisUDP-N-acetylmuramoyl-L-Ala-D-iGlu-meso-diaminopimeloyl-D-Ala-D-Ala0.0271.30.021 calc.pH 7.6Open
MraY (phospho-N-acetylmuramoyl-pentapeptide transferase)variant K48QBacillus subtilisundecaprenyl phosphate—0.26—pH 7.6Open
MraY (phospho-N-acetylmuramoyl-pentapeptide transferase)variant N168ABacillus subtilisUDP-N-acetylmuramoyl-L-Ala-D-iGlu-meso-diaminopimeloyl-D-Ala-D-Ala0.0720.950.075 calc.pH 7.6Open
MraY (phospho-N-acetylmuramoyl-pentapeptide transferase)variant N168ABacillus subtilisundecaprenyl phosphate—0.21—pH 7.6Open
MraY (phospho-N-acetylmuramoyl-pentapeptide transferase)variant N171ABacillus subtilisUDP-N-acetylmuramoyl-L-Ala-D-iGlu-meso-diaminopimeloyl-D-Ala-D-Ala0.01350.730.018 calc.pH 7.6Open
MraY (phospho-N-acetylmuramoyl-pentapeptide transferase)variant N171ABacillus subtilisundecaprenyl phosphate—0.24—pH 7.6Open