EC 3.1.1.101: poly(ethylene terephthalate) hydrolase
HydrolasesOpen in Browse
CatLog read 17 records for EC 3.1.1.101 from 5 papers, for 5 organisms. Names in these papers: cutinase Thc_Cut1, Est119 (cutinase-type polyesterase), IsPETase (PET hydrolase), Cut190* (cutinase-like PET hydrolase Cut190_S226P/R228S) and 2 more.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units.
CatLog also holds 33 records for EC 3.1.1.101 that are not yet re-read: from its earlier version, built from the enzyme databases and partly checked against the papers. All records for EC 3.1.1.101 in Browse.
Papers
| Paper | Organisms | Records |
|---|---|---|
| Enhanced cutinase-catalyzed hydrolysis of polyethylene terephthalate by covalent fusion to hydrophobins | Thermobifida cellulosilytica | 8 |
| Biochemical and genetic analysis of a cutinase-type polyesterase from a thermophilic Thermobifida alba AHK119 | Thermobifida alba | 4 |
| Catalytic Features and Thermal Adaptation Mechanisms of a Deep Sea Bacterial Cutinase-Type Poly(Ethylene Terephthalate) Hydrolase | Ideonella sakaiensis | 2 |
| doi:10.1016/j.molcatb.2010.01.001 | Thermobifida fusca | 2 |
| Enzymatic hydrolysis of PET: functional roles of three Ca2+ ions bound to a cutinase-like enzyme, Cut190*, and its engineering for improved activity | Saccharomonospora viridis | 1 |
Records
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Paper | Open record |
|---|---|---|---|---|---|---|---|---|
| Cut190* (cutinase-like PET hydrolase Cut190_S226P/R228S)variant S226P/R228S/Q138A/D250C/E296C | Saccharomonospora viridis | poly(ethylene terephthalate) | 1.6 | 5.7 | 0.279 | pH 8.5, 65 °C | PMID 30250976 | Open record: Cut190* (cutinase-like PET hydrolase Cut190_S226P/R228S) variant S226P/R228S/Q138A/D250C/E296C, poly(ethylene terephthalate) |
| cutinase Tfu_0882 | Thermobifida fusca | p-nitrophenyl butyrate | 483 | 0.673 | 700 | pH 8, 60 °C | doi:10.1016/j.molcatb.2010.01.001 | Open record: cutinase Tfu_0882, p-nitrophenyl butyrate |
| cutinase Tfu_0883 | Thermobifida fusca | p-nitrophenyl butyrate | 742 | 0.505 | 1.5 × 10³ | pH 8, 60 °C | doi:10.1016/j.molcatb.2010.01.001 | Open record: cutinase Tfu_0883, p-nitrophenyl butyrate |
| cutinase Thc_Cut1 | Thermobifida cellulosilytica | p-nitrophenyl acetate | 363 | 1.4 | 260 calc. | pH 7, 25 °C | PMID 25795674 | Open record: cutinase Thc_Cut1, p-nitrophenyl acetate |
| cutinase Thc_Cut1 | Thermobifida cellulosilytica | p-nitrophenyl butyrate | 325 | 0.8 | 406 | pH 7, 25 °C | PMID 25795674 | Open record: cutinase Thc_Cut1, p-nitrophenyl butyrate |
| cutinase Thc_Cut1Thc_Cut1_hfb4 (fusion to hydrophobin HFB4) | Thermobifida cellulosilytica | p-nitrophenyl acetate | 99 | 2.4 | 42 | pH 7, 25 °C | PMID 25795674 | Open record: cutinase Thc_Cut1 Thc_Cut1_hfb4 (fusion to hydrophobin HFB4), p-nitrophenyl acetate |
| cutinase Thc_Cut1Thc_Cut1_hfb4 (fusion to hydrophobin HFB4) | Thermobifida cellulosilytica | p-nitrophenyl butyrate | 86 | 1.3 | 68 | pH 7, 25 °C | PMID 25795674 | Open record: cutinase Thc_Cut1 Thc_Cut1_hfb4 (fusion to hydrophobin HFB4), p-nitrophenyl butyrate |
| cutinase Thc_Cut1Thc_Cut1_hfb7 (fusion to hydrophobin HFB7) | Thermobifida cellulosilytica | p-nitrophenyl acetate | 129 | 2.3 | 55 | pH 7, 25 °C | PMID 25795674 | Open record: cutinase Thc_Cut1 Thc_Cut1_hfb7 (fusion to hydrophobin HFB7), p-nitrophenyl acetate |
| cutinase Thc_Cut1Thc_Cut1_hfb7 (fusion to hydrophobin HFB7) | Thermobifida cellulosilytica | p-nitrophenyl butyrate | 112 | 0.9 | 130 | pH 7, 25 °C | PMID 25795674 | Open record: cutinase Thc_Cut1 Thc_Cut1_hfb7 (fusion to hydrophobin HFB7), p-nitrophenyl butyrate |
| cutinase Thc_Cut1Thc_Cut1_hfb9b (fusion to hydrophobin HFB9b) | Thermobifida cellulosilytica | p-nitrophenyl acetate | 105 | 1.5 | 71 | pH 7, 25 °C | PMID 25795674 | Open record: cutinase Thc_Cut1 Thc_Cut1_hfb9b (fusion to hydrophobin HFB9b), p-nitrophenyl acetate |
| cutinase Thc_Cut1Thc_Cut1_hfb9b (fusion to hydrophobin HFB9b) | Thermobifida cellulosilytica | p-nitrophenyl butyrate | 49 | 0.8 | 62 | pH 7, 25 °C | PMID 25795674 | Open record: cutinase Thc_Cut1 Thc_Cut1_hfb9b (fusion to hydrophobin HFB9b), p-nitrophenyl butyrate |
| Est119 (cutinase-type polyesterase) | Thermobifida alba | 4-nitrophenyl acetate | 2.06 | 3.22 | 0.640 calc. | 37 °C | PMID 22183084 | Open record: Est119 (cutinase-type polyesterase), 4-nitrophenyl acetate |
| Est119 (cutinase-type polyesterase) | Thermobifida alba | 4-nitrophenyl butyrate | 4.48 | 3.41 | 1.31 calc. | 37 °C | PMID 22183084 | Open record: Est119 (cutinase-type polyesterase), 4-nitrophenyl butyrate |
| Est119 (cutinase-type polyesterase) | Thermobifida alba | 4-nitrophenyl hexanoate | 3.28 | 2.37 | 1.38 calc. | 37 °C | PMID 22183084 | Open record: Est119 (cutinase-type polyesterase), 4-nitrophenyl hexanoate |
| Est119 (cutinase-type polyesterase) | Thermobifida alba | 4-nitrophenyl octanoate | 2.45 | 1.87 | 1.31 calc. | 37 °C | PMID 22183084 | Open record: Est119 (cutinase-type polyesterase), 4-nitrophenyl octanoate |
| IsPETase (PET hydrolase) | Ideonella sakaiensis | bis(2-hydroxyethyl) terephthalate | 5.95 | 4.48 | 1.33 | pH 8, 40 °C | PMID 35557867 | Open record: IsPETase (PET hydrolase), bis(2-hydroxyethyl) terephthalate |
| IsPETase (PET hydrolase) | Ideonella sakaiensis | mono(2-hydroxyethyl) terephthalate | 0.0139 | 0.72 | 0.01919 | pH 8, 40 °C | PMID 35557867 | Open record: IsPETase (PET hydrolase), mono(2-hydroxyethyl) terephthalate |