Values CatLog read from this paper

Enzymatic hydrolysis of PET: functional roles of three Ca²⁺ ions bound to a cutinase-like enzyme, Cut190*, and its engineering for improved activity

PMID 30250976doi:10.1007/s00253-018-9374-xOpen in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 20 records (60 values) from this paper: Cut190* (cutinase-like PET hydrolase Cut190_S226P/R228S) from Saccharomonospora viridis.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.

EC 3.1.1.74, EC 3.1.1.101

Records read from this paper
Enzyme	Organism	Substrate	k_cat (s⁻¹)	K_m (mM)	k_cat/K_m (mM⁻¹ s⁻¹)	Conditions	Open record
Cut190* (cutinase-like PET hydrolase Cut190_S226P/R228S)variant S226P/R228S + Ser inserted between F77 and A78 (78S)	Saccharomonospora viridis	poly(butylene succinate-co-adipate)2.5 mM Ca2+	151	0.12	1.26 × 10³	pH 8.2, 37 °C	Open record: Cut190* (cutinase-like PET hydrolase Cut190_S226P/R228S) variant S226P/R228S + Ser inserted between F77 and A78 (78S), poly(butylene succinate-co-adipate)
Cut190* (cutinase-like PET hydrolase Cut190_S226P/R228S)variant S226P/R228S + Ser inserted between F77 and A78 (78S)	Saccharomonospora viridis	poly(butylene succinate-co-adipate)in the absence of Ca2+	8	0.03	268	pH 8.2, 37 °C	Open record: Cut190* (cutinase-like PET hydrolase Cut190_S226P/R228S) variant S226P/R228S + Ser inserted between F77 and A78 (78S), poly(butylene succinate-co-adipate)
Cut190* (cutinase-like PET hydrolase Cut190_S226P/R228S)variant S226P/R228S/D250C/E296C	Saccharomonospora viridis	poly(butylene succinate-co-adipate)	50	0.15	334	pH 8.2, 37 °C	Open record: Cut190* (cutinase-like PET hydrolase Cut190_S226P/R228S) variant S226P/R228S/D250C/E296C, poly(butylene succinate-co-adipate)
Cut190* (cutinase-like PET hydrolase Cut190_S226P/R228S)variant S226P/R228S/E184R	Saccharomonospora viridis	poly(butylene succinate-co-adipate)2.5 mM Ca2+	60	0.17	353	pH 8.2, 37 °C	Open record: Cut190* (cutinase-like PET hydrolase Cut190_S226P/R228S) variant S226P/R228S/E184R, poly(butylene succinate-co-adipate)
Cut190* (cutinase-like PET hydrolase Cut190_S226P/R228S)variant S226P/R228S/E184R	Saccharomonospora viridis	poly(butylene succinate-co-adipate)in the absence of Ca2+	38	0.096	395	pH 8.2, 37 °C	Open record: Cut190* (cutinase-like PET hydrolase Cut190_S226P/R228S) variant S226P/R228S/E184R, poly(butylene succinate-co-adipate)
Cut190* (cutinase-like PET hydrolase Cut190_S226P/R228S)variant S226P/R228S/N133D	Saccharomonospora viridis	poly(butylene succinate-co-adipate)	25	0.079	317	pH 8.2, 37 °C	Open record: Cut190* (cutinase-like PET hydrolase Cut190_S226P/R228S) variant S226P/R228S/N133D, poly(butylene succinate-co-adipate)
Cut190* (cutinase-like PET hydrolase Cut190_S226P/R228S)variant S226P/R228S/N168A	Saccharomonospora viridis	poly(butylene succinate-co-adipate)	43	0.1	430	pH 8.2, 37 °C	Open record: Cut190* (cutinase-like PET hydrolase Cut190_S226P/R228S) variant S226P/R228S/N168A, poly(butylene succinate-co-adipate)
Cut190* (cutinase-like PET hydrolase Cut190_S226P/R228S)variant S226P/R228S/N168D	Saccharomonospora viridis	poly(butylene succinate-co-adipate)	30	0.091	330	pH 8.2, 37 °C	Open record: Cut190* (cutinase-like PET hydrolase Cut190_S226P/R228S) variant S226P/R228S/N168D, poly(butylene succinate-co-adipate)
Cut190* (cutinase-like PET hydrolase Cut190_S226P/R228S)variant S226P/R228S/N202H	Saccharomonospora viridis	poly(butylene succinate-co-adipate)	60	0.16	375	pH 8.2, 37 °C	Open record: Cut190* (cutinase-like PET hydrolase Cut190_S226P/R228S) variant S226P/R228S/N202H, poly(butylene succinate-co-adipate)
Cut190* (cutinase-like PET hydrolase Cut190_S226P/R228S)variant S226P/R228S/Q110E	Saccharomonospora viridis	poly(butylene succinate-co-adipate)	27	0.07	390	pH 8.2, 37 °C	Open record: Cut190* (cutinase-like PET hydrolase Cut190_S226P/R228S) variant S226P/R228S/Q110E, poly(butylene succinate-co-adipate)
Cut190* (cutinase-like PET hydrolase Cut190_S226P/R228S)variant S226P/R228S/Q123H	Saccharomonospora viridis	poly(butylene succinate-co-adipate)	61	0.14	435	pH 8.2, 37 °C	Open record: Cut190* (cutinase-like PET hydrolase Cut190_S226P/R228S) variant S226P/R228S/Q123H, poly(butylene succinate-co-adipate)
Cut190* (cutinase-like PET hydrolase Cut190_S226P/R228S)variant S226P/R228S/Q138A/D250C/E296C	Saccharomonospora viridis	poly(butylene succinate-co-adipate)	150	0.39	385	pH 8.2, 37 °C	Open record: Cut190* (cutinase-like PET hydrolase Cut190_S226P/R228S) variant S226P/R228S/Q138A/D250C/E296C, poly(butylene succinate-co-adipate)
Cut190* (cutinase-like PET hydrolase Cut190_S226P/R228S)variant S226P/R228S/Q138A/D250C/E296C	Saccharomonospora viridis	poly(ethylene terephthalate)	1.6	5.7	0.279	pH 8.5, 65 °C	Open record: Cut190* (cutinase-like PET hydrolase Cut190_S226P/R228S) variant S226P/R228S/Q138A/D250C/E296C, poly(ethylene terephthalate)
Cut190* (cutinase-like PET hydrolase Cut190_S226P/R228S)variant S226P/R228S/Q138A/D250C/E296C/E220R	Saccharomonospora viridis	poly(butylene succinate-co-adipate)	150	0.48	313	pH 8.2, 37 °C	Open record: Cut190* (cutinase-like PET hydrolase Cut190_S226P/R228S) variant S226P/R228S/Q138A/D250C/E296C/E220R, poly(butylene succinate-co-adipate)
Cut190* (cutinase-like PET hydrolase Cut190_S226P/R228S)variant S226P/R228S/Q138A/D250C/E296C/E220R/G251D	Saccharomonospora viridis	poly(butylene succinate-co-adipate)	75	0.18	417	pH 8.2, 37 °C	Open record: Cut190* (cutinase-like PET hydrolase Cut190_S226P/R228S) variant S226P/R228S/Q138A/D250C/E296C/E220R/G251D, poly(butylene succinate-co-adipate)
Cut190* (cutinase-like PET hydrolase Cut190_S226P/R228S)variant S226P/R228S/Q138A/D250C/E296C/E220R/G251D/Q123H/N202H	Saccharomonospora viridis	poly(butylene succinate-co-adipate)	60	0.17	353	pH 8.2, 37 °C	Open record: Cut190* (cutinase-like PET hydrolase Cut190_S226P/R228S) variant S226P/R228S/Q138A/D250C/E296C/E220R/G251D/Q123H/N202H, poly(butylene succinate-co-adipate)
Cut190* (cutinase-like PET hydrolase Cut190_S226P/R228S)variant S226P/R228S/Q138A/D250C/E296C/Q123H/N202H	Saccharomonospora viridis	poly(butylene succinate-co-adipate)	100	0.32	313	pH 8.2, 37 °C	Open record: Cut190* (cutinase-like PET hydrolase Cut190_S226P/R228S) variant S226P/R228S/Q138A/D250C/E296C/Q123H/N202H, poly(butylene succinate-co-adipate)
Cut190* (cutinase-like PET hydrolase Cut190_S226P/R228S)variant S226P/R228S/Q138A/Q141E	Saccharomonospora viridis	poly(butylene succinate-co-adipate)	19	0.045	427	pH 8.2, 37 °C	Open record: Cut190* (cutinase-like PET hydrolase Cut190_S226P/R228S) variant S226P/R228S/Q138A/Q141E, poly(butylene succinate-co-adipate)
Cut190* (cutinase-like PET hydrolase Cut190_S226P/R228S)variant S226P/R228S/Q141E	Saccharomonospora viridis	poly(butylene succinate-co-adipate)	50	0.11	456	pH 8.2, 37 °C	Open record: Cut190* (cutinase-like PET hydrolase Cut190_S226P/R228S) variant S226P/R228S/Q141E, poly(butylene succinate-co-adipate)
Cut190* (cutinase-like PET hydrolase Cut190_S226P/R228S)variant S226P/R228S/Q209E	Saccharomonospora viridis	poly(butylene succinate-co-adipate)	75	0.2	377	pH 8.2, 37 °C	Open record: Cut190* (cutinase-like PET hydrolase Cut190_S226P/R228S) variant S226P/R228S/Q209E, poly(butylene succinate-co-adipate)