EC 3.1.1.74: cutinase

HydrolasesOpen in Browse

CatLog read 131 records for EC 3.1.1.74 from 10 papers, for 12 organisms. Names in these papers: cutinase, Cut190* (cutinase-like PET hydrolase Cut190_S226P/R228S), cutinase Thc_Cut2, MtCut (cutinase-type PET hydrolase) and 12 more.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units.

CatLog also holds 135 records for EC 3.1.1.74 that are not yet re-read: from its earlier version, built from the enzyme databases and partly checked against the papers. All records for EC 3.1.1.74 in Browse.

Papers

Papers with records for EC 3.1.1.74
Paper	Organisms	Records
Phylogenetic analysis and in-depth characterization of functionally and structurally diverse CE5 cutinases	Coprinopsis cinerea, Cryptococcus sp and 3 more	25
Biochemical and structural characterisation of cutinase mutants in the presence of the anionic surfactant AOT	Fusarium solani pisi	20
Surface engineering of a cutinase from Thermobifida cellulosilytica for improved polyester hydrolysis	Thermobifida cellulosilytica	20
Enzymatic hydrolysis of PET: functional roles of three Ca²⁺ ions bound to a cutinase-like enzyme, Cut190*, and its engineering for improved activity	Saccharomonospora viridis	19
Catalytic Features and Thermal Adaptation Mechanisms of a Deep Sea Bacterial Cutinase-Type Poly(Ethylene Terephthalate) Hydrolase	Marinactinospora thermotolerans, uncultured bacterium	13
Substrate specificity and kinetic properties of enzymes belonging to the hormone-sensitive lipase family: comparison with non-lipolytic and lipolytic carboxylesterases	Fusarium solani	10
Crystal structure and thermodynamic and kinetic stability of metagenome-derived LC-cutinase	—	9
Enhanced cutinase-catalyzed hydrolysis of polyethylene terephthalate by covalent fusion to hydrophobins	Thermobifida cellulosilytica	8
Biochemical and genetic analysis of a cutinase-type polyesterase from a thermophilic Thermobifida alba AHK119	Thermobifida alba	4
doi:10.1016/j.molcatb.2010.01.001	Fusarium solani pisi, Thermobifida fusca	3

Records

Records for EC 3.1.1.74 read from the paper
Enzyme	Organism	Substrate	k_cat (s⁻¹)	K_m (mM)	k_cat/K_m (mM⁻¹ s⁻¹)	Conditions	Paper	Open record
CcCut	Coprinopsis cinerea	4-nitrophenyl acetate	41.37	34.31	1.21	pH 7, 40 °C	PMID 34653507	Open record: CcCut, 4-nitrophenyl acetate
CcCut	Coprinopsis cinerea	4-nitrophenyl butanoate	8.96	3.56	2.52	pH 7, 40 °C	PMID 34653507	Open record: CcCut, 4-nitrophenyl butanoate
CcCut	Coprinopsis cinerea	4-nitrophenyl dodecanoate	0.22	2.76	0.08	pH 7, 40 °C	PMID 34653507	Open record: CcCut, 4-nitrophenyl dodecanoate
CcCut	Coprinopsis cinerea	4-nitrophenyl hexadecanoate	0.06	2.9	0.02	pH 7, 40 °C	PMID 34653507	Open record: CcCut, 4-nitrophenyl hexadecanoate
CcCut	Coprinopsis cinerea	4-nitrophenyl octanoate	1.7	1.67	1.02	pH 7, 40 °C	PMID 34653507	Open record: CcCut, 4-nitrophenyl octanoate
CspCut	Cryptococcus sp	4-nitrophenyl acetate	29.74	9.54	3.13	pH 7, 40 °C	PMID 34653507	Open record: CspCut, 4-nitrophenyl acetate
CspCut	Cryptococcus sp	4-nitrophenyl butanoate	13.52	5.54	2.51	pH 7, 40 °C	PMID 34653507	Open record: CspCut, 4-nitrophenyl butanoate
CspCut	Cryptococcus sp	4-nitrophenyl dodecanoate	3.49	0.05	65.97	pH 7, 40 °C	PMID 34653507	Open record: CspCut, 4-nitrophenyl dodecanoate
CspCut	Cryptococcus sp	4-nitrophenyl hexadecanoate	3.38	0.05	70.68	pH 7, 40 °C	PMID 34653507	Open record: CspCut, 4-nitrophenyl hexadecanoate
CspCut	Cryptococcus sp	4-nitrophenyl octanoate	3.81	0.05	83.2	pH 7, 40 °C	PMID 34653507	Open record: CspCut, 4-nitrophenyl octanoate
Cut190* (cutinase-like PET hydrolase Cut190_S226P/R228S)variant S226P/R228S + Ser inserted between F77 and A78 (78S)	Saccharomonospora viridis	poly(butylene succinate-co-adipate)2.5 mM Ca2+	151	0.12	1.26 × 10³	pH 8.2, 37 °C	PMID 30250976	Open record: Cut190* (cutinase-like PET hydrolase Cut190_S226P/R228S) variant S226P/R228S + Ser inserted between F77 and A78 (78S), poly(butylene succinate-co-adipate)
Cut190* (cutinase-like PET hydrolase Cut190_S226P/R228S)variant S226P/R228S + Ser inserted between F77 and A78 (78S)	Saccharomonospora viridis	poly(butylene succinate-co-adipate)in the absence of Ca2+	8	0.03	268	pH 8.2, 37 °C	PMID 30250976	Open record: Cut190* (cutinase-like PET hydrolase Cut190_S226P/R228S) variant S226P/R228S + Ser inserted between F77 and A78 (78S), poly(butylene succinate-co-adipate)
Cut190* (cutinase-like PET hydrolase Cut190_S226P/R228S)variant S226P/R228S/D250C/E296C	Saccharomonospora viridis	poly(butylene succinate-co-adipate)	50	0.15	334	pH 8.2, 37 °C	PMID 30250976	Open record: Cut190* (cutinase-like PET hydrolase Cut190_S226P/R228S) variant S226P/R228S/D250C/E296C, poly(butylene succinate-co-adipate)
Cut190* (cutinase-like PET hydrolase Cut190_S226P/R228S)variant S226P/R228S/E184R	Saccharomonospora viridis	poly(butylene succinate-co-adipate)2.5 mM Ca2+	60	0.17	353	pH 8.2, 37 °C	PMID 30250976	Open record: Cut190* (cutinase-like PET hydrolase Cut190_S226P/R228S) variant S226P/R228S/E184R, poly(butylene succinate-co-adipate)
Cut190* (cutinase-like PET hydrolase Cut190_S226P/R228S)variant S226P/R228S/E184R	Saccharomonospora viridis	poly(butylene succinate-co-adipate)in the absence of Ca2+	38	0.096	395	pH 8.2, 37 °C	PMID 30250976	Open record: Cut190* (cutinase-like PET hydrolase Cut190_S226P/R228S) variant S226P/R228S/E184R, poly(butylene succinate-co-adipate)
Cut190* (cutinase-like PET hydrolase Cut190_S226P/R228S)variant S226P/R228S/N133D	Saccharomonospora viridis	poly(butylene succinate-co-adipate)	25	0.079	317	pH 8.2, 37 °C	PMID 30250976	Open record: Cut190* (cutinase-like PET hydrolase Cut190_S226P/R228S) variant S226P/R228S/N133D, poly(butylene succinate-co-adipate)
Cut190* (cutinase-like PET hydrolase Cut190_S226P/R228S)variant S226P/R228S/N168A	Saccharomonospora viridis	poly(butylene succinate-co-adipate)	43	0.1	430	pH 8.2, 37 °C	PMID 30250976	Open record: Cut190* (cutinase-like PET hydrolase Cut190_S226P/R228S) variant S226P/R228S/N168A, poly(butylene succinate-co-adipate)
Cut190* (cutinase-like PET hydrolase Cut190_S226P/R228S)variant S226P/R228S/N168D	Saccharomonospora viridis	poly(butylene succinate-co-adipate)	30	0.091	330	pH 8.2, 37 °C	PMID 30250976	Open record: Cut190* (cutinase-like PET hydrolase Cut190_S226P/R228S) variant S226P/R228S/N168D, poly(butylene succinate-co-adipate)
Cut190* (cutinase-like PET hydrolase Cut190_S226P/R228S)variant S226P/R228S/N202H	Saccharomonospora viridis	poly(butylene succinate-co-adipate)	60	0.16	375	pH 8.2, 37 °C	PMID 30250976	Open record: Cut190* (cutinase-like PET hydrolase Cut190_S226P/R228S) variant S226P/R228S/N202H, poly(butylene succinate-co-adipate)
Cut190* (cutinase-like PET hydrolase Cut190_S226P/R228S)variant S226P/R228S/Q110E	Saccharomonospora viridis	poly(butylene succinate-co-adipate)	27	0.07	390	pH 8.2, 37 °C	PMID 30250976	Open record: Cut190* (cutinase-like PET hydrolase Cut190_S226P/R228S) variant S226P/R228S/Q110E, poly(butylene succinate-co-adipate)
Cut190* (cutinase-like PET hydrolase Cut190_S226P/R228S)variant S226P/R228S/Q123H	Saccharomonospora viridis	poly(butylene succinate-co-adipate)	61	0.14	435	pH 8.2, 37 °C	PMID 30250976	Open record: Cut190* (cutinase-like PET hydrolase Cut190_S226P/R228S) variant S226P/R228S/Q123H, poly(butylene succinate-co-adipate)
Cut190* (cutinase-like PET hydrolase Cut190_S226P/R228S)variant S226P/R228S/Q138A/D250C/E296C	Saccharomonospora viridis	poly(butylene succinate-co-adipate)	150	0.39	385	pH 8.2, 37 °C	PMID 30250976	Open record: Cut190* (cutinase-like PET hydrolase Cut190_S226P/R228S) variant S226P/R228S/Q138A/D250C/E296C, poly(butylene succinate-co-adipate)
Cut190* (cutinase-like PET hydrolase Cut190_S226P/R228S)variant S226P/R228S/Q138A/D250C/E296C/E220R	Saccharomonospora viridis	poly(butylene succinate-co-adipate)	150	0.48	313	pH 8.2, 37 °C	PMID 30250976	Open record: Cut190* (cutinase-like PET hydrolase Cut190_S226P/R228S) variant S226P/R228S/Q138A/D250C/E296C/E220R, poly(butylene succinate-co-adipate)
Cut190* (cutinase-like PET hydrolase Cut190_S226P/R228S)variant S226P/R228S/Q138A/D250C/E296C/E220R/G251D	Saccharomonospora viridis	poly(butylene succinate-co-adipate)	75	0.18	417	pH 8.2, 37 °C	PMID 30250976	Open record: Cut190* (cutinase-like PET hydrolase Cut190_S226P/R228S) variant S226P/R228S/Q138A/D250C/E296C/E220R/G251D, poly(butylene succinate-co-adipate)
Cut190* (cutinase-like PET hydrolase Cut190_S226P/R228S)variant S226P/R228S/Q138A/D250C/E296C/E220R/G251D/Q123H/N202H	Saccharomonospora viridis	poly(butylene succinate-co-adipate)	60	0.17	353	pH 8.2, 37 °C	PMID 30250976	Open record: Cut190* (cutinase-like PET hydrolase Cut190_S226P/R228S) variant S226P/R228S/Q138A/D250C/E296C/E220R/G251D/Q123H/N202H, poly(butylene succinate-co-adipate)
Cut190* (cutinase-like PET hydrolase Cut190_S226P/R228S)variant S226P/R228S/Q138A/D250C/E296C/Q123H/N202H	Saccharomonospora viridis	poly(butylene succinate-co-adipate)	100	0.32	313	pH 8.2, 37 °C	PMID 30250976	Open record: Cut190* (cutinase-like PET hydrolase Cut190_S226P/R228S) variant S226P/R228S/Q138A/D250C/E296C/Q123H/N202H, poly(butylene succinate-co-adipate)
Cut190* (cutinase-like PET hydrolase Cut190_S226P/R228S)variant S226P/R228S/Q138A/Q141E	Saccharomonospora viridis	poly(butylene succinate-co-adipate)	19	0.045	427	pH 8.2, 37 °C	PMID 30250976	Open record: Cut190* (cutinase-like PET hydrolase Cut190_S226P/R228S) variant S226P/R228S/Q138A/Q141E, poly(butylene succinate-co-adipate)
Cut190* (cutinase-like PET hydrolase Cut190_S226P/R228S)variant S226P/R228S/Q141E	Saccharomonospora viridis	poly(butylene succinate-co-adipate)	50	0.11	456	pH 8.2, 37 °C	PMID 30250976	Open record: Cut190* (cutinase-like PET hydrolase Cut190_S226P/R228S) variant S226P/R228S/Q141E, poly(butylene succinate-co-adipate)
Cut190* (cutinase-like PET hydrolase Cut190_S226P/R228S)variant S226P/R228S/Q209E	Saccharomonospora viridis	poly(butylene succinate-co-adipate)	75	0.2	377	pH 8.2, 37 °C	PMID 30250976	Open record: Cut190* (cutinase-like PET hydrolase Cut190_S226P/R228S) variant S226P/R228S/Q209E, poly(butylene succinate-co-adipate)
cutinase	Fusarium solani	4-nitrophenyl butanoate	525	—	—	pH 7.5, 25 °C	PMID 16325466	Open record: cutinase, 4-nitrophenyl butanoate
cutinase	Fusarium solani	ethenyl acetate	240	—	—	pH 7.5, 25 °C	PMID 16325466	Open record: cutinase, ethenyl acetate
cutinase	Fusarium solani	ethenyl butanoate	50	—	—	pH 7.5, 25 °C	PMID 16325466	Open record: cutinase, ethenyl butanoate
cutinase	Fusarium solani	ethenyl dodecanoate	285	—	—	pH 7.5, 25 °C	PMID 16325466	Open record: cutinase, ethenyl dodecanoate
cutinase	Fusarium solani	ethenyl propanoate	255	—	—	pH 7.5, 25 °C	PMID 16325466	Open record: cutinase, ethenyl propanoate
cutinase	Fusarium solani	glycerol triacetate	115	—	—	pH 7.5, 25 °C	PMID 16325466	Open record: cutinase, glycerol triacetate
cutinase	Fusarium solani	glycerol tributanoate	130	—	—	pH 7.5, 25 °C	PMID 16325466	Open record: cutinase, glycerol tributanoate
cutinase	Fusarium solani	glycerol trioctanoate	305	—	—	pH 7.5, 25 °C	PMID 16325466	Open record: cutinase, glycerol trioctanoate
cutinase	Fusarium solani	glycerol tripropanoate	210	—	—	pH 7.5, 25 °C	PMID 16325466	Open record: cutinase, glycerol tripropanoate
cutinase	Fusarium solani	Olive oil	245	—	—	pH 7.5, 25 °C	PMID 16325466	Open record: cutinase, Olive oil
cutinase	Fusarium solani pisi	p-nitrophenyl butyrate0 mM (dioctyl sulfosuccinate sodium salt, below CMC)	1.56 × 10⁴	0.35	4.5 × 10⁴ calc.	pH 8	PMID 18501723	Open record: cutinase, p-nitrophenyl butyrate
cutinase	Fusarium solani pisi	p-nitrophenyl butyrate0.5 mM (dioctyl sulfosuccinate sodium salt, below CMC)	1.55 × 10⁴	0.48	3.2 × 10⁴ calc.	pH 8	PMID 18501723	Open record: cutinase, p-nitrophenyl butyrate
cutinase	Fusarium solani pisi	p-nitrophenyl butyrate1 mM (dioctyl sulfosuccinate sodium salt, below CMC)	1.74 × 10⁴	0.85	2.0 × 10⁴ calc.	pH 8	PMID 18501723	Open record: cutinase, p-nitrophenyl butyrate
cutinase	Fusarium solani pisi	p-nitrophenyl butyrate1.5 mM (dioctyl sulfosuccinate sodium salt, below CMC)	1.52 × 10⁴	1.12	1.36 × 10⁴ calc.	pH 8	PMID 18501723	Open record: cutinase, p-nitrophenyl butyrate
cutinase	Fusarium solani pisi	p-nitrophenyl butyrate2 mM (dioctyl sulfosuccinate sodium salt, below CMC)	1.38 × 10⁴	1.08	1.28 × 10⁴ calc.	pH 8	PMID 18501723	Open record: cutinase, p-nitrophenyl butyrate
cutinasevariant L153Q	Fusarium solani pisi	p-nitrophenyl butyrate0 mM (dioctyl sulfosuccinate sodium salt, below CMC)	1.1 × 10⁴	0.31	3.5 × 10⁴ calc.	pH 8	PMID 18501723	Open record: cutinase variant L153Q, p-nitrophenyl butyrate
cutinasevariant L153Q	Fusarium solani pisi	p-nitrophenyl butyrate0.5 mM (dioctyl sulfosuccinate sodium salt, below CMC)	1.6 × 10⁴	0.69	2.3 × 10⁴ calc.	pH 8	PMID 18501723	Open record: cutinase variant L153Q, p-nitrophenyl butyrate
cutinasevariant L153Q	Fusarium solani pisi	p-nitrophenyl butyrate1 mM (dioctyl sulfosuccinate sodium salt, below CMC)	1.79 × 10⁴	1.31	1.37 × 10⁴ calc.	pH 8	PMID 18501723	Open record: cutinase variant L153Q, p-nitrophenyl butyrate
cutinasevariant L153Q	Fusarium solani pisi	p-nitrophenyl butyrate1.5 mM (dioctyl sulfosuccinate sodium salt, below CMC)	2.58 × 10⁴	3.57	7.23 × 10³ calc.	pH 8	PMID 18501723	Open record: cutinase variant L153Q, p-nitrophenyl butyrate
cutinasevariant L153Q	Fusarium solani pisi	p-nitrophenyl butyrate2 mM (dioctyl sulfosuccinate sodium salt, below CMC)	2.85 × 10⁴	4.33	6.58 × 10³ calc.	pH 8	PMID 18501723	Open record: cutinase variant L153Q, p-nitrophenyl butyrate
cutinasevariant S54D	Fusarium solani pisi	p-nitrophenyl butyrate0 mM (dioctyl sulfosuccinate sodium salt, below CMC)	1.47 × 10⁴	0.33	4.5 × 10⁴ calc.	pH 8	PMID 18501723	Open record: cutinase variant S54D, p-nitrophenyl butyrate
cutinasevariant S54D	Fusarium solani pisi	p-nitrophenyl butyrate0.5 mM (dioctyl sulfosuccinate sodium salt, below CMC)	2.47 × 10⁴	0.74	3.3 × 10⁴ calc.	pH 8	PMID 18501723	Open record: cutinase variant S54D, p-nitrophenyl butyrate
cutinasevariant S54D	Fusarium solani pisi	p-nitrophenyl butyrate1 mM (dioctyl sulfosuccinate sodium salt, below CMC)	2.42 × 10⁴	0.72	3.4 × 10⁴ calc.	pH 8	PMID 18501723	Open record: cutinase variant S54D, p-nitrophenyl butyrate
cutinasevariant S54D	Fusarium solani pisi	p-nitrophenyl butyrate1.5 mM (dioctyl sulfosuccinate sodium salt, below CMC)	2.52 × 10⁴	1.14	2.21 × 10⁴ calc.	pH 8	PMID 18501723	Open record: cutinase variant S54D, p-nitrophenyl butyrate
cutinasevariant S54D	Fusarium solani pisi	p-nitrophenyl butyrate2 mM (dioctyl sulfosuccinate sodium salt, below CMC)	3.21 × 10⁴	1.74	1.84 × 10⁴ calc.	pH 8	PMID 18501723	Open record: cutinase variant S54D, p-nitrophenyl butyrate
cutinasevariant T179C	Fusarium solani pisi	p-nitrophenyl butyrate0 mM (dioctyl sulfosuccinate sodium salt, below CMC)	7.9 × 10³	0.2	4.0 × 10⁴ calc.	pH 8	PMID 18501723	Open record: cutinase variant T179C, p-nitrophenyl butyrate
cutinasevariant T179C	Fusarium solani pisi	p-nitrophenyl butyrate0.5 mM (dioctyl sulfosuccinate sodium salt, below CMC)	9.6 × 10³	0.49	2.0 × 10⁴ calc.	pH 8	PMID 18501723	Open record: cutinase variant T179C, p-nitrophenyl butyrate
cutinasevariant T179C	Fusarium solani pisi	p-nitrophenyl butyrate1 mM (dioctyl sulfosuccinate sodium salt, below CMC)	1.11 × 10⁴	0.66	1.7 × 10⁴ calc.	pH 8	PMID 18501723	Open record: cutinase variant T179C, p-nitrophenyl butyrate
cutinasevariant T179C	Fusarium solani pisi	p-nitrophenyl butyrate1.5 mM (dioctyl sulfosuccinate sodium salt, below CMC)	1.1 × 10⁴	1.09	1.0 × 10⁴ calc.	pH 8	PMID 18501723	Open record: cutinase variant T179C, p-nitrophenyl butyrate
cutinasevariant T179C	Fusarium solani pisi	p-nitrophenyl butyrate2 mM (dioctyl sulfosuccinate sodium salt, below CMC)	1.15 × 10⁴	1.56	7.37 × 10³ calc.	pH 8	PMID 18501723	Open record: cutinase variant T179C, p-nitrophenyl butyrate
cutinase FspC	Fusarium solani pisi	p-nitrophenyl butyrate	837	0.272	3.2 × 10³	pH 8, 40 °C	doi:10.1016/j.molcatb.2010.01.001	Open record: cutinase FspC, p-nitrophenyl butyrate
cutinase Tfu_0882	Thermobifida fusca	p-nitrophenyl butyrate	483	0.673	700	pH 8, 60 °C	doi:10.1016/j.molcatb.2010.01.001	Open record: cutinase Tfu_0882, p-nitrophenyl butyrate
cutinase Tfu_0883	Thermobifida fusca	p-nitrophenyl butyrate	742	0.505	1.5 × 10³	pH 8, 60 °C	doi:10.1016/j.molcatb.2010.01.001	Open record: cutinase Tfu_0883, p-nitrophenyl butyrate
cutinase Thc_Cut1	Thermobifida cellulosilytica	4-nitrophenyl acetate	436	1.5	291	pH 7, 25 °C	PMID 23592055	Open record: cutinase Thc_Cut1, 4-nitrophenyl acetate
cutinase Thc_Cut1	Thermobifida cellulosilytica	4-nitrophenyl butyrate	327	0.8	425	pH 7, 25 °C	PMID 23592055	Open record: cutinase Thc_Cut1, 4-nitrophenyl butyrate
cutinase Thc_Cut1	Thermobifida cellulosilytica	p-nitrophenyl acetate	363	1.4	260 calc.	pH 7, 25 °C	PMID 25795674	Open record: cutinase Thc_Cut1, p-nitrophenyl acetate
cutinase Thc_Cut1	Thermobifida cellulosilytica	p-nitrophenyl butyrate	325	0.8	406	pH 7, 25 °C	PMID 25795674	Open record: cutinase Thc_Cut1, p-nitrophenyl butyrate
cutinase Thc_Cut1Thc_Cut1_hfb4 (fusion to hydrophobin HFB4)	Thermobifida cellulosilytica	p-nitrophenyl acetate	99	2.4	42	pH 7, 25 °C	PMID 25795674	Open record: cutinase Thc_Cut1 Thc_Cut1_hfb4 (fusion to hydrophobin HFB4), p-nitrophenyl acetate
cutinase Thc_Cut1Thc_Cut1_hfb4 (fusion to hydrophobin HFB4)	Thermobifida cellulosilytica	p-nitrophenyl butyrate	86	1.3	68	pH 7, 25 °C	PMID 25795674	Open record: cutinase Thc_Cut1 Thc_Cut1_hfb4 (fusion to hydrophobin HFB4), p-nitrophenyl butyrate
cutinase Thc_Cut1Thc_Cut1_hfb7 (fusion to hydrophobin HFB7)	Thermobifida cellulosilytica	p-nitrophenyl acetate	129	2.3	55	pH 7, 25 °C	PMID 25795674	Open record: cutinase Thc_Cut1 Thc_Cut1_hfb7 (fusion to hydrophobin HFB7), p-nitrophenyl acetate
cutinase Thc_Cut1Thc_Cut1_hfb7 (fusion to hydrophobin HFB7)	Thermobifida cellulosilytica	p-nitrophenyl butyrate	112	0.9	130	pH 7, 25 °C	PMID 25795674	Open record: cutinase Thc_Cut1 Thc_Cut1_hfb7 (fusion to hydrophobin HFB7), p-nitrophenyl butyrate
cutinase Thc_Cut1Thc_Cut1_hfb9b (fusion to hydrophobin HFB9b)	Thermobifida cellulosilytica	p-nitrophenyl acetate	105	1.5	71	pH 7, 25 °C	PMID 25795674	Open record: cutinase Thc_Cut1 Thc_Cut1_hfb9b (fusion to hydrophobin HFB9b), p-nitrophenyl acetate
cutinase Thc_Cut1Thc_Cut1_hfb9b (fusion to hydrophobin HFB9b)	Thermobifida cellulosilytica	p-nitrophenyl butyrate	49	0.8	62	pH 7, 25 °C	PMID 25795674	Open record: cutinase Thc_Cut1 Thc_Cut1_hfb9b (fusion to hydrophobin HFB9b), p-nitrophenyl butyrate
cutinase Thc_Cut2	Thermobifida cellulosilytica	4-nitrophenyl acetate	17	1.9	9	pH 7, 25 °C	PMID 23592055	Open record: cutinase Thc_Cut2, 4-nitrophenyl acetate
cutinase Thc_Cut2	Thermobifida cellulosilytica	4-nitrophenyl butyrate	16	3.4	5	pH 7, 25 °C	PMID 23592055	Open record: cutinase Thc_Cut2, 4-nitrophenyl butyrate
cutinase Thc_Cut2variant A30V	Thermobifida cellulosilytica	4-nitrophenyl acetate	260	1.7	153	pH 7, 25 °C	PMID 23592055	Open record: cutinase Thc_Cut2 variant A30V, 4-nitrophenyl acetate
cutinase Thc_Cut2variant A30V	Thermobifida cellulosilytica	4-nitrophenyl butyrate	183	1.1	171	pH 7, 25 °C	PMID 23592055	Open record: cutinase Thc_Cut2 variant A30V, 4-nitrophenyl butyrate
cutinase Thc_Cut2variant L183A	Thermobifida cellulosilytica	4-nitrophenyl acetate	25	1.9	13	pH 7, 25 °C	PMID 23592055	Open record: cutinase Thc_Cut2 variant L183A, 4-nitrophenyl acetate
cutinase Thc_Cut2variant L183A	Thermobifida cellulosilytica	4-nitrophenyl butyrate	32	2.1	15	pH 7, 25 °C	PMID 23592055	Open record: cutinase Thc_Cut2 variant L183A, 4-nitrophenyl butyrate
cutinase Thc_Cut2variant Q65E	Thermobifida cellulosilytica	4-nitrophenyl acetate	7	1.5	5	pH 7, 25 °C	PMID 23592055	Open record: cutinase Thc_Cut2 variant Q65E, 4-nitrophenyl acetate
cutinase Thc_Cut2variant Q65E	Thermobifida cellulosilytica	4-nitrophenyl butyrate	15	2.6	6	pH 7, 25 °C	PMID 23592055	Open record: cutinase Thc_Cut2 variant Q65E, 4-nitrophenyl butyrate
cutinase Thc_Cut2variant R187K	Thermobifida cellulosilytica	4-nitrophenyl acetate	35	0.8	44	pH 7, 25 °C	PMID 23592055	Open record: cutinase Thc_Cut2 variant R187K, 4-nitrophenyl acetate
cutinase Thc_Cut2variant R187K	Thermobifida cellulosilytica	4-nitrophenyl butyrate	79	4	20	pH 7, 25 °C	PMID 23592055	Open record: cutinase Thc_Cut2 variant R187K, 4-nitrophenyl butyrate
cutinase Thc_Cut2variant R19S	Thermobifida cellulosilytica	4-nitrophenyl acetate	36	1.2	30	pH 7, 25 °C	PMID 23592055	Open record: cutinase Thc_Cut2 variant R19S, 4-nitrophenyl acetate
cutinase Thc_Cut2variant R19S	Thermobifida cellulosilytica	4-nitrophenyl butyrate	80	2.2	36	pH 7, 25 °C	PMID 23592055	Open record: cutinase Thc_Cut2 variant R19S, 4-nitrophenyl butyrate
cutinase Thc_Cut2variant R19S/R29N/A30V	Thermobifida cellulosilytica	4-nitrophenyl acetate	76	1.2	65	pH 7, 25 °C	PMID 23592055	Open record: cutinase Thc_Cut2 variant R19S/R29N/A30V, 4-nitrophenyl acetate
cutinase Thc_Cut2variant R19S/R29N/A30V	Thermobifida cellulosilytica	4-nitrophenyl butyrate	136	1.4	100	pH 7, 25 °C	PMID 23592055	Open record: cutinase Thc_Cut2 variant R19S/R29N/A30V, 4-nitrophenyl butyrate
cutinase Thc_Cut2variant R29N	Thermobifida cellulosilytica	4-nitrophenyl acetate	207	1.3	159	pH 7, 25 °C	PMID 23592055	Open record: cutinase Thc_Cut2 variant R29N, 4-nitrophenyl acetate
cutinase Thc_Cut2variant R29N	Thermobifida cellulosilytica	4-nitrophenyl butyrate	191	2	95	pH 7, 25 °C	PMID 23592055	Open record: cutinase Thc_Cut2 variant R29N, 4-nitrophenyl butyrate
cutinase Thc_Cut2variant R29N/A30V	Thermobifida cellulosilytica	4-nitrophenyl acetate	100	1.3	76	pH 7, 25 °C	PMID 23592055	Open record: cutinase Thc_Cut2 variant R29N/A30V, 4-nitrophenyl acetate
cutinase Thc_Cut2variant R29N/A30V	Thermobifida cellulosilytica	4-nitrophenyl butyrate	173	1	168	pH 7, 25 °C	PMID 23592055	Open record: cutinase Thc_Cut2 variant R29N/A30V, 4-nitrophenyl butyrate
Est119 (cutinase-type polyesterase)	Thermobifida alba	4-nitrophenyl acetate	2.06	3.22	0.640 calc.	37 °C	PMID 22183084	Open record: Est119 (cutinase-type polyesterase), 4-nitrophenyl acetate
Est119 (cutinase-type polyesterase)	Thermobifida alba	4-nitrophenyl butyrate	4.48	3.41	1.31 calc.	37 °C	PMID 22183084	Open record: Est119 (cutinase-type polyesterase), 4-nitrophenyl butyrate
Est119 (cutinase-type polyesterase)	Thermobifida alba	4-nitrophenyl hexanoate	3.28	2.37	1.38 calc.	37 °C	PMID 22183084	Open record: Est119 (cutinase-type polyesterase), 4-nitrophenyl hexanoate
Est119 (cutinase-type polyesterase)	Thermobifida alba	4-nitrophenyl octanoate	2.45	1.87	1.31 calc.	37 °C	PMID 22183084	Open record: Est119 (cutinase-type polyesterase), 4-nitrophenyl octanoate
FsCut	Fusarium solani	4-nitrophenyl acetate	29.96	7.31	4.1	pH 7, 40 °C	PMID 34653507	Open record: FsCut, 4-nitrophenyl acetate
FsCut	Fusarium solani	4-nitrophenyl butanoate	7.7	1.67	5.71	pH 7, 40 °C	PMID 34653507	Open record: FsCut, 4-nitrophenyl butanoate
FsCut	Fusarium solani	4-nitrophenyl dodecanoate	1.22	0.05	22.78	pH 7, 40 °C	PMID 34653507	Open record: FsCut, 4-nitrophenyl dodecanoate
FsCut	Fusarium solani	4-nitrophenyl hexadecanoate	0.15	0.01	10.6	pH 7, 40 °C	PMID 34653507	Open record: FsCut, 4-nitrophenyl hexadecanoate
FsCut	Fusarium solani	4-nitrophenyl octanoate	6.69	0.72	9.25	pH 7, 40 °C	PMID 34653507	Open record: FsCut, 4-nitrophenyl octanoate
ICCG (engineered leaf-branch compost cutinase LCC)variant ICCG	uncultured bacterium	bis(2-hydroxyethyl) terephthalate	2.29	2.58	1	pH 8, 40 °C	PMID 35557867	Open record: ICCG (engineered leaf-branch compost cutinase LCC) variant ICCG, bis(2-hydroxyethyl) terephthalate
ICCG (engineered leaf-branch compost cutinase LCC)variant ICCG	uncultured bacterium	mono(2-hydroxyethyl) terephthalate	0.0032	0.43	0.00675	pH 8, 40 °C	PMID 35557867	Open record: ICCG (engineered leaf-branch compost cutinase LCC) variant ICCG, mono(2-hydroxyethyl) terephthalate
LC-cutinase (metagenome-derived cutinase from leaf-branch compost)	—	p-nitrophenyl butyrateno PEG	232	0.22	1.05 × 10³	pH 8, 30 °C	PMID 24593046	Open record: LC-cutinase (metagenome-derived cutinase from leaf-branch compost), p-nitrophenyl butyrate
LC-cutinase (metagenome-derived cutinase from leaf-branch compost)	—	p-nitrophenyl butyrate1% PEG 1000	278	0.27	1046	pH 8, 30 °C	PMID 24593046	Open record: LC-cutinase (metagenome-derived cutinase from leaf-branch compost), p-nitrophenyl butyrate
LC-cutinase (metagenome-derived cutinase from leaf-branch compost)	—	p-nitrophenyl butyrateno PEG	343	0.21	1.63 × 10³	pH 8, 50 °C	PMID 24593046	Open record: LC-cutinase (metagenome-derived cutinase from leaf-branch compost), p-nitrophenyl butyrate
LC-cutinase (metagenome-derived cutinase from leaf-branch compost)	—	p-nitrophenyl butyrate1% PEG 1000	359	0.27	1311	pH 8, 50 °C	PMID 24593046	Open record: LC-cutinase (metagenome-derived cutinase from leaf-branch compost), p-nitrophenyl butyrate
LC-cutinase (metagenome-derived cutinase from leaf-branch compost)	—	p-nitrophenyl butyrateno PEG	213	0.24	888	pH 8, 70 °C	PMID 24593046	Open record: LC-cutinase (metagenome-derived cutinase from leaf-branch compost), p-nitrophenyl butyrate
LC-cutinase (metagenome-derived cutinase from leaf-branch compost)	—	p-nitrophenyl butyrate1% PEG 1000	318	0.25	1295	pH 8, 70 °C	PMID 24593046	Open record: LC-cutinase (metagenome-derived cutinase from leaf-branch compost), p-nitrophenyl butyrate
LC-cutinase (metagenome-derived cutinase from leaf-branch compost)variant C275A/C292A	—	p-nitrophenyl butyrate	342	0.25	1.37 × 10³	pH 8, 30 °C	PMID 24593046	Open record: LC-cutinase (metagenome-derived cutinase from leaf-branch compost) variant C275A/C292A, p-nitrophenyl butyrate
LC-cutinase (metagenome-derived cutinase from leaf-branch compost)variant C275A/C292A	—	p-nitrophenyl butyrate	196	0.18	1.09 × 10³	pH 8, 50 °C	PMID 24593046	Open record: LC-cutinase (metagenome-derived cutinase from leaf-branch compost) variant C275A/C292A, p-nitrophenyl butyrate
LC-cutinase (metagenome-derived cutinase from leaf-branch compost)variant C275A/C292A	—	p-nitrophenyl butyrate	49	0.19	258	pH 8, 70 °C	PMID 24593046	Open record: LC-cutinase (metagenome-derived cutinase from leaf-branch compost) variant C275A/C292A, p-nitrophenyl butyrate
MtCut (cutinase-type PET hydrolase)	Marinactinospora thermotolerans	4-nitrophenyl acetate	4.47	0.55	8.19	pH 8, 30 °C	PMID 35557867	Open record: MtCut (cutinase-type PET hydrolase), 4-nitrophenyl acetate
MtCut (cutinase-type PET hydrolase)	Marinactinospora thermotolerans	4-nitrophenyl butanoate	14.1	1.33	10.6	pH 8, 30 °C	PMID 35557867	Open record: MtCut (cutinase-type PET hydrolase), 4-nitrophenyl butanoate
MtCut (cutinase-type PET hydrolase)	Marinactinospora thermotolerans	4-nitrophenyl decanoate	69.85	4.93	14.2	pH 8, 30 °C	PMID 35557867	Open record: MtCut (cutinase-type PET hydrolase), 4-nitrophenyl decanoate
MtCut (cutinase-type PET hydrolase)	Marinactinospora thermotolerans	4-nitrophenyl dodecanoate	30.52	1.68	18.1	pH 8, 30 °C	PMID 35557867	Open record: MtCut (cutinase-type PET hydrolase), 4-nitrophenyl dodecanoate
MtCut (cutinase-type PET hydrolase)	Marinactinospora thermotolerans	4-nitrophenyl hexadecanoate	4.79	0.15	32.5	pH 8, 30 °C	PMID 35557867	Open record: MtCut (cutinase-type PET hydrolase), 4-nitrophenyl hexadecanoate
MtCut (cutinase-type PET hydrolase)	Marinactinospora thermotolerans	4-nitrophenyl hexanoate	41.72	4.61	9.23	pH 8, 30 °C	PMID 35557867	Open record: MtCut (cutinase-type PET hydrolase), 4-nitrophenyl hexanoate
MtCut (cutinase-type PET hydrolase)	Marinactinospora thermotolerans	4-nitrophenyl octadecanoate	14.31	0.45	31.8	pH 8, 30 °C	PMID 35557867	Open record: MtCut (cutinase-type PET hydrolase), 4-nitrophenyl octadecanoate
MtCut (cutinase-type PET hydrolase)	Marinactinospora thermotolerans	4-nitrophenyl octanoate	79.39	2.55	31.1	pH 8, 30 °C	PMID 35557867	Open record: MtCut (cutinase-type PET hydrolase), 4-nitrophenyl octanoate
MtCut (cutinase-type PET hydrolase)	Marinactinospora thermotolerans	4-nitrophenyl tetradecanoate	9.87	0.19	51.4	pH 8, 30 °C	PMID 35557867	Open record: MtCut (cutinase-type PET hydrolase), 4-nitrophenyl tetradecanoate
MtCut (cutinase-type PET hydrolase)	Marinactinospora thermotolerans	bis(2-hydroxyethyl) terephthalate	4.27	3.07	1.41	pH 8, 40 °C	PMID 35557867	Open record: MtCut (cutinase-type PET hydrolase), bis(2-hydroxyethyl) terephthalate
MtCut (cutinase-type PET hydrolase)	Marinactinospora thermotolerans	mono(2-hydroxyethyl) terephthalate	0.13	0.75	0.176	pH 8, 40 °C	PMID 35557867	Open record: MtCut (cutinase-type PET hydrolase), mono(2-hydroxyethyl) terephthalate
PbCut	Pyrenopeziza brassicae	4-nitrophenyl acetate	11.84	11.73	1.01	pH 7, 40 °C	PMID 34653507	Open record: PbCut, 4-nitrophenyl acetate
PbCut	Pyrenopeziza brassicae	4-nitrophenyl butanoate	6.45	1.71	3.79	pH 7, 40 °C	PMID 34653507	Open record: PbCut, 4-nitrophenyl butanoate
PbCut	Pyrenopeziza brassicae	4-nitrophenyl dodecanoate	0.2	0.08	2.5	pH 7, 40 °C	PMID 34653507	Open record: PbCut, 4-nitrophenyl dodecanoate
PbCut	Pyrenopeziza brassicae	4-nitrophenyl hexadecanoate	0.08	0.04	2.07	pH 7, 40 °C	PMID 34653507	Open record: PbCut, 4-nitrophenyl hexadecanoate
PbCut	Pyrenopeziza brassicae	4-nitrophenyl octanoate	1.43	0.54	2.65	pH 7, 40 °C	PMID 34653507	Open record: PbCut, 4-nitrophenyl octanoate
TtCut	Thermothielavioides terrestris	4-nitrophenyl acetate	65.79	24.81	2.66	pH 7, 40 °C	PMID 34653507	Open record: TtCut, 4-nitrophenyl acetate
TtCut	Thermothielavioides terrestris	4-nitrophenyl butanoate	15.92	7.08	2.25	pH 7, 40 °C	PMID 34653507	Open record: TtCut, 4-nitrophenyl butanoate
TtCut	Thermothielavioides terrestris	4-nitrophenyl dodecanoate	0.07	0.21	0.31	pH 7, 40 °C	PMID 34653507	Open record: TtCut, 4-nitrophenyl dodecanoate
TtCut	Thermothielavioides terrestris	4-nitrophenyl hexadecanoate	0.09	0.5	0.19	pH 7, 40 °C	PMID 34653507	Open record: TtCut, 4-nitrophenyl hexadecanoate
TtCut	Thermothielavioides terrestris	4-nitrophenyl octanoate	2.3	0.39	5.81	pH 7, 40 °C	PMID 34653507	Open record: TtCut, 4-nitrophenyl octanoate