Values CatLog read from this paper
Surface engineering of a cutinase from Thermobifida cellulosilytica for improved polyester hydrolysis
PMID 23592055doi:10.1002/bit.24930Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 20 records (60 values) from this paper: cutinase Thc_Cut2, cutinase Thc_Cut1 from Thermobifida cellulosilytica.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| cutinase Thc_Cut1 | Thermobifida cellulosilytica | 4-nitrophenyl acetate | 436 | 1.5 | 291 | pH 7, 25 °C | Open record: cutinase Thc_Cut1, 4-nitrophenyl acetate |
| cutinase Thc_Cut1 | Thermobifida cellulosilytica | 4-nitrophenyl butyrate | 327 | 0.8 | 425 | pH 7, 25 °C | Open record: cutinase Thc_Cut1, 4-nitrophenyl butyrate |
| cutinase Thc_Cut2 | Thermobifida cellulosilytica | 4-nitrophenyl acetate | 17 | 1.9 | 9 | pH 7, 25 °C | Open record: cutinase Thc_Cut2, 4-nitrophenyl acetate |
| cutinase Thc_Cut2 | Thermobifida cellulosilytica | 4-nitrophenyl butyrate | 16 | 3.4 | 5 | pH 7, 25 °C | Open record: cutinase Thc_Cut2, 4-nitrophenyl butyrate |
| cutinase Thc_Cut2variant A30V | Thermobifida cellulosilytica | 4-nitrophenyl acetate | 260 | 1.7 | 153 | pH 7, 25 °C | Open record: cutinase Thc_Cut2 variant A30V, 4-nitrophenyl acetate |
| cutinase Thc_Cut2variant A30V | Thermobifida cellulosilytica | 4-nitrophenyl butyrate | 183 | 1.1 | 171 | pH 7, 25 °C | Open record: cutinase Thc_Cut2 variant A30V, 4-nitrophenyl butyrate |
| cutinase Thc_Cut2variant L183A | Thermobifida cellulosilytica | 4-nitrophenyl acetate | 25 | 1.9 | 13 | pH 7, 25 °C | Open record: cutinase Thc_Cut2 variant L183A, 4-nitrophenyl acetate |
| cutinase Thc_Cut2variant L183A | Thermobifida cellulosilytica | 4-nitrophenyl butyrate | 32 | 2.1 | 15 | pH 7, 25 °C | Open record: cutinase Thc_Cut2 variant L183A, 4-nitrophenyl butyrate |
| cutinase Thc_Cut2variant Q65E | Thermobifida cellulosilytica | 4-nitrophenyl acetate | 7 | 1.5 | 5 | pH 7, 25 °C | Open record: cutinase Thc_Cut2 variant Q65E, 4-nitrophenyl acetate |
| cutinase Thc_Cut2variant Q65E | Thermobifida cellulosilytica | 4-nitrophenyl butyrate | 15 | 2.6 | 6 | pH 7, 25 °C | Open record: cutinase Thc_Cut2 variant Q65E, 4-nitrophenyl butyrate |
| cutinase Thc_Cut2variant R187K | Thermobifida cellulosilytica | 4-nitrophenyl acetate | 35 | 0.8 | 44 | pH 7, 25 °C | Open record: cutinase Thc_Cut2 variant R187K, 4-nitrophenyl acetate |
| cutinase Thc_Cut2variant R187K | Thermobifida cellulosilytica | 4-nitrophenyl butyrate | 79 | 4 | 20 | pH 7, 25 °C | Open record: cutinase Thc_Cut2 variant R187K, 4-nitrophenyl butyrate |
| cutinase Thc_Cut2variant R19S | Thermobifida cellulosilytica | 4-nitrophenyl acetate | 36 | 1.2 | 30 | pH 7, 25 °C | Open record: cutinase Thc_Cut2 variant R19S, 4-nitrophenyl acetate |
| cutinase Thc_Cut2variant R19S | Thermobifida cellulosilytica | 4-nitrophenyl butyrate | 80 | 2.2 | 36 | pH 7, 25 °C | Open record: cutinase Thc_Cut2 variant R19S, 4-nitrophenyl butyrate |
| cutinase Thc_Cut2variant R19S/R29N/A30V | Thermobifida cellulosilytica | 4-nitrophenyl acetate | 76 | 1.2 | 65 | pH 7, 25 °C | Open record: cutinase Thc_Cut2 variant R19S/R29N/A30V, 4-nitrophenyl acetate |
| cutinase Thc_Cut2variant R19S/R29N/A30V | Thermobifida cellulosilytica | 4-nitrophenyl butyrate | 136 | 1.4 | 100 | pH 7, 25 °C | Open record: cutinase Thc_Cut2 variant R19S/R29N/A30V, 4-nitrophenyl butyrate |
| cutinase Thc_Cut2variant R29N | Thermobifida cellulosilytica | 4-nitrophenyl acetate | 207 | 1.3 | 159 | pH 7, 25 °C | Open record: cutinase Thc_Cut2 variant R29N, 4-nitrophenyl acetate |
| cutinase Thc_Cut2variant R29N | Thermobifida cellulosilytica | 4-nitrophenyl butyrate | 191 | 2 | 95 | pH 7, 25 °C | Open record: cutinase Thc_Cut2 variant R29N, 4-nitrophenyl butyrate |
| cutinase Thc_Cut2variant R29N/A30V | Thermobifida cellulosilytica | 4-nitrophenyl acetate | 100 | 1.3 | 76 | pH 7, 25 °C | Open record: cutinase Thc_Cut2 variant R29N/A30V, 4-nitrophenyl acetate |
| cutinase Thc_Cut2variant R29N/A30V | Thermobifida cellulosilytica | 4-nitrophenyl butyrate | 173 | 1 | 168 | pH 7, 25 °C | Open record: cutinase Thc_Cut2 variant R29N/A30V, 4-nitrophenyl butyrate |