Values CatLog read from this paper
Biochemical and structural characterisation of cutinase mutants in the presence of the anionic surfactant AOT
PMID 18501723doi:10.1016/j.bbapap.2008.04.017Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 20 records (40 values) from this paper: cutinase from Fusarium solani pisi.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 20 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| cutinase | Fusarium solani pisi | p-nitrophenyl butyrate0 mM (dioctyl sulfosuccinate sodium salt, below CMC) | 1.56 × 10⁴ | 0.35 | 4.5 × 10⁴ calc. | pH 8 | Open record: cutinase, p-nitrophenyl butyrate |
| cutinase | Fusarium solani pisi | p-nitrophenyl butyrate0.5 mM (dioctyl sulfosuccinate sodium salt, below CMC) | 1.55 × 10⁴ | 0.48 | 3.2 × 10⁴ calc. | pH 8 | Open record: cutinase, p-nitrophenyl butyrate |
| cutinase | Fusarium solani pisi | p-nitrophenyl butyrate1 mM (dioctyl sulfosuccinate sodium salt, below CMC) | 1.74 × 10⁴ | 0.85 | 2.0 × 10⁴ calc. | pH 8 | Open record: cutinase, p-nitrophenyl butyrate |
| cutinase | Fusarium solani pisi | p-nitrophenyl butyrate1.5 mM (dioctyl sulfosuccinate sodium salt, below CMC) | 1.52 × 10⁴ | 1.12 | 1.36 × 10⁴ calc. | pH 8 | Open record: cutinase, p-nitrophenyl butyrate |
| cutinase | Fusarium solani pisi | p-nitrophenyl butyrate2 mM (dioctyl sulfosuccinate sodium salt, below CMC) | 1.38 × 10⁴ | 1.08 | 1.28 × 10⁴ calc. | pH 8 | Open record: cutinase, p-nitrophenyl butyrate |
| cutinasevariant L153Q | Fusarium solani pisi | p-nitrophenyl butyrate0 mM (dioctyl sulfosuccinate sodium salt, below CMC) | 1.1 × 10⁴ | 0.31 | 3.5 × 10⁴ calc. | pH 8 | Open record: cutinase variant L153Q, p-nitrophenyl butyrate |
| cutinasevariant L153Q | Fusarium solani pisi | p-nitrophenyl butyrate0.5 mM (dioctyl sulfosuccinate sodium salt, below CMC) | 1.6 × 10⁴ | 0.69 | 2.3 × 10⁴ calc. | pH 8 | Open record: cutinase variant L153Q, p-nitrophenyl butyrate |
| cutinasevariant L153Q | Fusarium solani pisi | p-nitrophenyl butyrate1 mM (dioctyl sulfosuccinate sodium salt, below CMC) | 1.79 × 10⁴ | 1.31 | 1.37 × 10⁴ calc. | pH 8 | Open record: cutinase variant L153Q, p-nitrophenyl butyrate |
| cutinasevariant L153Q | Fusarium solani pisi | p-nitrophenyl butyrate1.5 mM (dioctyl sulfosuccinate sodium salt, below CMC) | 2.58 × 10⁴ | 3.57 | 7.23 × 10³ calc. | pH 8 | Open record: cutinase variant L153Q, p-nitrophenyl butyrate |
| cutinasevariant L153Q | Fusarium solani pisi | p-nitrophenyl butyrate2 mM (dioctyl sulfosuccinate sodium salt, below CMC) | 2.85 × 10⁴ | 4.33 | 6.58 × 10³ calc. | pH 8 | Open record: cutinase variant L153Q, p-nitrophenyl butyrate |
| cutinasevariant S54D | Fusarium solani pisi | p-nitrophenyl butyrate0 mM (dioctyl sulfosuccinate sodium salt, below CMC) | 1.47 × 10⁴ | 0.33 | 4.5 × 10⁴ calc. | pH 8 | Open record: cutinase variant S54D, p-nitrophenyl butyrate |
| cutinasevariant S54D | Fusarium solani pisi | p-nitrophenyl butyrate0.5 mM (dioctyl sulfosuccinate sodium salt, below CMC) | 2.47 × 10⁴ | 0.74 | 3.3 × 10⁴ calc. | pH 8 | Open record: cutinase variant S54D, p-nitrophenyl butyrate |
| cutinasevariant S54D | Fusarium solani pisi | p-nitrophenyl butyrate1 mM (dioctyl sulfosuccinate sodium salt, below CMC) | 2.42 × 10⁴ | 0.72 | 3.4 × 10⁴ calc. | pH 8 | Open record: cutinase variant S54D, p-nitrophenyl butyrate |
| cutinasevariant S54D | Fusarium solani pisi | p-nitrophenyl butyrate1.5 mM (dioctyl sulfosuccinate sodium salt, below CMC) | 2.52 × 10⁴ | 1.14 | 2.21 × 10⁴ calc. | pH 8 | Open record: cutinase variant S54D, p-nitrophenyl butyrate |
| cutinasevariant S54D | Fusarium solani pisi | p-nitrophenyl butyrate2 mM (dioctyl sulfosuccinate sodium salt, below CMC) | 3.21 × 10⁴ | 1.74 | 1.84 × 10⁴ calc. | pH 8 | Open record: cutinase variant S54D, p-nitrophenyl butyrate |
| cutinasevariant T179C | Fusarium solani pisi | p-nitrophenyl butyrate0 mM (dioctyl sulfosuccinate sodium salt, below CMC) | 7.9 × 10³ | 0.2 | 4.0 × 10⁴ calc. | pH 8 | Open record: cutinase variant T179C, p-nitrophenyl butyrate |
| cutinasevariant T179C | Fusarium solani pisi | p-nitrophenyl butyrate0.5 mM (dioctyl sulfosuccinate sodium salt, below CMC) | 9.6 × 10³ | 0.49 | 2.0 × 10⁴ calc. | pH 8 | Open record: cutinase variant T179C, p-nitrophenyl butyrate |
| cutinasevariant T179C | Fusarium solani pisi | p-nitrophenyl butyrate1 mM (dioctyl sulfosuccinate sodium salt, below CMC) | 1.11 × 10⁴ | 0.66 | 1.7 × 10⁴ calc. | pH 8 | Open record: cutinase variant T179C, p-nitrophenyl butyrate |
| cutinasevariant T179C | Fusarium solani pisi | p-nitrophenyl butyrate1.5 mM (dioctyl sulfosuccinate sodium salt, below CMC) | 1.1 × 10⁴ | 1.09 | 1.0 × 10⁴ calc. | pH 8 | Open record: cutinase variant T179C, p-nitrophenyl butyrate |
| cutinasevariant T179C | Fusarium solani pisi | p-nitrophenyl butyrate2 mM (dioctyl sulfosuccinate sodium salt, below CMC) | 1.15 × 10⁴ | 1.56 | 7.37 × 10³ calc. | pH 8 | Open record: cutinase variant T179C, p-nitrophenyl butyrate |