CatLogenzyme kinetics catalog

EC 3.1.4.35: 3',5'-cyclic-GMP phosphodiesterase

HydrolasesOpen in Browse

CatLog read 15 records for EC 3.1.4.35 from 2 papers, for 1 organism. Names in these papers: phosphodiesterase-5 (hPDE5A1), PDE9A2.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units.

CatLog also holds 52 records for EC 3.1.4.35 that are not yet re-read: from its earlier version, built from the enzyme databases and partly checked against the papers. All records for EC 3.1.4.35 in Browse.

Papers

PaperOrganismsRecords
Critical amino acids in phosphodiesterase-5 catalytic site that provide for high-affinity interaction with cyclic guanosine monophosphate and inhibitorsHomo sapiens9
Structural asymmetry of phosphodiesterase-9, potential protonation of a glutamic acid, and role of the invariant glutamineHomo sapiens6

Records

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)ConditionsPaper
PDE9A2Homo sapiensadenosine 3',5'-cyclic monophosphate2.370.5014.73 calc.pH 8.2PMID 21483814Open
PDE9A2Homo sapiensguanosine 3',5'-cyclic monophosphate0.181.13 × 10⁻⁴1.6 × 10³ calc.pH 8.2PMID 21483814Open
PDE9A2variant E406AHomo sapiensadenosine 3',5'-cyclic monophosphate0.310.3380.92 calc.pH 8.2PMID 21483814Open
PDE9A2variant E406AHomo sapiensguanosine 3',5'-cyclic monophosphate0.083.11 × 10⁻⁴260 calc.pH 8.2PMID 21483814Open
PDE9A2variant Q453EHomo sapiensadenosine 3',5'-cyclic monophosphate1.31.2331.1 calc.pH 8.2PMID 21483814Open
PDE9A2variant Q453EHomo sapiensguanosine 3',5'-cyclic monophosphate0.090.002832 calc.pH 8.2PMID 21483814Open
phosphodiesterase-5 (hPDE5A1)Homo sapiensguanosine 3',5'-cyclic monophosphate2.20.0029758pH 7.5, 30 °CPMID 17979301Open
phosphodiesterase-5 (hPDE5A1)variant F786AHomo sapiensguanosine 3',5'-cyclic monophosphate3.70.0297124pH 7.5, 30 °CPMID 17979301Open
phosphodiesterase-5 (hPDE5A1)variant F820AHomo sapiensguanosine 3',5'-cyclic monophosphate0.040.1720.23pH 7.5, 30 °CPMID 17979301Open
phosphodiesterase-5 (hPDE5A1)variant H613AHomo sapiensguanosine 3',5'-cyclic monophosphate8.3 × 10⁻⁴0.00710.012pH 7.5, 30 °CPMID 17979301Open
phosphodiesterase-5 (hPDE5A1)variant L765AHomo sapiensguanosine 3',5'-cyclic monophosphate1.30.027747pH 7.5, 30 °CPMID 17979301Open
phosphodiesterase-5 (hPDE5A1)variant Q817AHomo sapiensguanosine 3',5'-cyclic monophosphate3.80.18221pH 7.5, 30 °CPMID 17979301Open
phosphodiesterase-5 (hPDE5A1)variant V782AHomo sapiensguanosine 3',5'-cyclic monophosphate2.50.0159157pH 7.5, 30 °CPMID 17979301Open
phosphodiesterase-5 (hPDE5A1)variant Y612AHomo sapiensguanosine 3',5'-cyclic monophosphate0.80.041619pH 7.5, 30 °CPMID 17979301Open
phosphodiesterase-5 (hPDE5A1)variant Y612FHomo sapiensguanosine 3',5'-cyclic monophosphate1.70.0061272pH 7.5, 30 °CPMID 17979301Open