Values CatLog read from this paper
Critical amino acids in phosphodiesterase-5 catalytic site that provide for high-affinity interaction with cyclic guanosine monophosphate and inhibitors
PMID 17979301doi:10.1021/bi7010702Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 9 records (27 values) from this paper: phosphodiesterase-5 (hPDE5A1) from Homo sapiens.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| phosphodiesterase-5 (hPDE5A1) | Homo sapiens | guanosine 3',5'-cyclic monophosphate | 2.2 | 0.0029 | 758 | pH 7.5, 30 °C | Open record: phosphodiesterase-5 (hPDE5A1), guanosine 3',5'-cyclic monophosphate |
| phosphodiesterase-5 (hPDE5A1)variant F786A | Homo sapiens | guanosine 3',5'-cyclic monophosphate | 3.7 | 0.0297 | 124 | pH 7.5, 30 °C | Open record: phosphodiesterase-5 (hPDE5A1) variant F786A, guanosine 3',5'-cyclic monophosphate |
| phosphodiesterase-5 (hPDE5A1)variant F820A | Homo sapiens | guanosine 3',5'-cyclic monophosphate | 0.04 | 0.172 | 0.23 | pH 7.5, 30 °C | Open record: phosphodiesterase-5 (hPDE5A1) variant F820A, guanosine 3',5'-cyclic monophosphate |
| phosphodiesterase-5 (hPDE5A1)variant H613A | Homo sapiens | guanosine 3',5'-cyclic monophosphate | 8.3 × 10⁻⁴ | 0.0071 | 0.012 | pH 7.5, 30 °C | Open record: phosphodiesterase-5 (hPDE5A1) variant H613A, guanosine 3',5'-cyclic monophosphate |
| phosphodiesterase-5 (hPDE5A1)variant L765A | Homo sapiens | guanosine 3',5'-cyclic monophosphate | 1.3 | 0.0277 | 47 | pH 7.5, 30 °C | Open record: phosphodiesterase-5 (hPDE5A1) variant L765A, guanosine 3',5'-cyclic monophosphate |
| phosphodiesterase-5 (hPDE5A1)variant Q817A | Homo sapiens | guanosine 3',5'-cyclic monophosphate | 3.8 | 0.182 | 21 | pH 7.5, 30 °C | Open record: phosphodiesterase-5 (hPDE5A1) variant Q817A, guanosine 3',5'-cyclic monophosphate |
| phosphodiesterase-5 (hPDE5A1)variant V782A | Homo sapiens | guanosine 3',5'-cyclic monophosphate | 2.5 | 0.0159 | 157 | pH 7.5, 30 °C | Open record: phosphodiesterase-5 (hPDE5A1) variant V782A, guanosine 3',5'-cyclic monophosphate |
| phosphodiesterase-5 (hPDE5A1)variant Y612A | Homo sapiens | guanosine 3',5'-cyclic monophosphate | 0.8 | 0.0416 | 19 | pH 7.5, 30 °C | Open record: phosphodiesterase-5 (hPDE5A1) variant Y612A, guanosine 3',5'-cyclic monophosphate |
| phosphodiesterase-5 (hPDE5A1)variant Y612F | Homo sapiens | guanosine 3',5'-cyclic monophosphate | 1.7 | 0.0061 | 272 | pH 7.5, 30 °C | Open record: phosphodiesterase-5 (hPDE5A1) variant Y612F, guanosine 3',5'-cyclic monophosphate |