CatLogenzyme kinetics catalog

EC 3.1.4.54: N-acetylphosphatidylethanolamine-hydrolysing phospholipase D

HydrolasesOpen in Browse

CatLog read 20 records for EC 3.1.4.54 from 1 paper, for 3 organisms. Names in these papers: NAPE-PLD (N-acylphosphatidylethanolamine-hydrolyzing phospholipase D).

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units.

CatLog also holds 4 records for EC 3.1.4.54 that are not yet re-read: from its earlier version, built from the enzyme databases and partly checked against the papers. All records for EC 3.1.4.54 in Browse.

Papers

PaperOrganismsRecords
Functional analysis of the purified anandamide-generating phospholipase D as a member of the metallo-beta-lactamase familyHomo sapiens, Mus musculus and 1 more20

Records

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)ConditionsPaper
NAPE-PLD (N-acylphosphatidylethanolamine-hydrolyzing phospholipase D)Homo sapiensN-palmitoyl-1,2-dioleoylphosphatidylethanolamine—0.0046—pH 7.4, 37 °CPMID 16527816Open
NAPE-PLD (N-acylphosphatidylethanolamine-hydrolyzing phospholipase D)variant D389NHomo sapiensN-palmitoyl-1,2-dioleoylphosphatidylethanolamine—0.0029—pH 7.4, 37 °CPMID 16527816Open
NAPE-PLD (N-acylphosphatidylethanolamine-hydrolyzing phospholipase D)variant S152AHomo sapiensN-palmitoyl-1,2-dioleoylphosphatidylethanolamine—0.0037—pH 7.4, 37 °CPMID 16527816Open
NAPE-PLD (N-acylphosphatidylethanolamine-hydrolyzing phospholipase D)Mus musculusN-palmitoyl-1,2-dioleoylphosphatidylethanolamine—0.0045—pH 7.4, 37 °CPMID 16527816Open
NAPE-PLD (N-acylphosphatidylethanolamine-hydrolyzing phospholipase D)variant 387stopMus musculusN-palmitoyl-1,2-dioleoylphosphatidylethanolamine—0.0036—pH 7.4, 37 °CPMID 16527816Open
NAPE-PLD (N-acylphosphatidylethanolamine-hydrolyzing phospholipase D)variant ΔN55Mus musculusN-palmitoyl-1,2-dioleoylphosphatidylethanolamine—0.0029—pH 7.4, 37 °CPMID 16527816Open
NAPE-PLD (N-acylphosphatidylethanolamine-hydrolyzing phospholipase D)Rattus norvegicusN-palmitoyl-1,2-dioleoylphosphatidylethanolaminepurified recombinant enzyme (3.1 ng); 10 mM MgCl2; purified—0.0017—pH 7.4, 37 °CPMID 16527816Open
NAPE-PLD (N-acylphosphatidylethanolamine-hydrolyzing phospholipase D)Rattus norvegicusN-palmitoyl-1,2-dioleoylphosphatidylethanolaminepurified recombinant enzyme (31 ng); 5 mM EDTA (Mg2+-free)—0.0059—pH 7.4, 37 °CPMID 16527816Open
NAPE-PLD (N-acylphosphatidylethanolamine-hydrolyzing phospholipase D)Rattus norvegicusN-palmitoyl-1,2-dioleoylphosphatidylethanolamine10 mM MgCl2; expressed in COS-7 cells (pcDNA3.1(+))—0.0047—pH 7.4, 37 °CPMID 16527816Open
NAPE-PLD (N-acylphosphatidylethanolamine-hydrolyzing phospholipase D)variant C170SRattus norvegicusN-palmitoyl-1,2-dioleoylphosphatidylethanolamine—0.0047—pH 7.4, 37 °CPMID 16527816Open
NAPE-PLD (N-acylphosphatidylethanolamine-hydrolyzing phospholipase D)variant C222SRattus norvegicusN-palmitoyl-1,2-dioleoylphosphatidylethanolamine—0.0043—pH 7.4, 37 °CPMID 16527816Open
NAPE-PLD (N-acylphosphatidylethanolamine-hydrolyzing phospholipase D)variant C224SRattus norvegicusN-palmitoyl-1,2-dioleoylphosphatidylethanolamine—0.0143—pH 7.4, 37 °CPMID 16527816Open
NAPE-PLD (N-acylphosphatidylethanolamine-hydrolyzing phospholipase D)variant C237SRattus norvegicusN-palmitoyl-1,2-dioleoylphosphatidylethanolamine—0.0042—pH 7.4, 37 °CPMID 16527816Open
NAPE-PLD (N-acylphosphatidylethanolamine-hydrolyzing phospholipase D)variant C255SRattus norvegicusN-palmitoyl-1,2-dioleoylphosphatidylethanolamine—0.0061—pH 7.4, 37 °CPMID 16527816Open
NAPE-PLD (N-acylphosphatidylethanolamine-hydrolyzing phospholipase D)variant C288SRattus norvegicusN-palmitoyl-1,2-dioleoylphosphatidylethanolamine—0.0045—pH 7.4, 37 °CPMID 16527816Open
NAPE-PLD (N-acylphosphatidylethanolamine-hydrolyzing phospholipase D)variant H331NRattus norvegicusN-palmitoyl-1,2-dioleoylphosphatidylethanolamine—0.0211—pH 7.4, 37 °CPMID 16527816Open
NAPE-PLD (N-acylphosphatidylethanolamine-hydrolyzing phospholipase D)variant H343NRattus norvegicusN-palmitoyl-1,2-dioleoylphosphatidylethanolamine—0.0039—pH 7.4, 37 °CPMID 16527816Open
NAPE-PLD (N-acylphosphatidylethanolamine-hydrolyzing phospholipase D)variant H353NRattus norvegicusN-palmitoyl-1,2-dioleoylphosphatidylethanolamine—0.0055—pH 7.4, 37 °CPMID 16527816Open
NAPE-PLD (N-acylphosphatidylethanolamine-hydrolyzing phospholipase D)variant H380RRattus norvegicusN-palmitoyl-1,2-dioleoylphosphatidylethanolamine—0.0142—pH 7.4, 37 °CPMID 16527816Open
NAPE-PLD (N-acylphosphatidylethanolamine-hydrolyzing phospholipase D)variant L207FRattus norvegicusN-palmitoyl-1,2-dioleoylphosphatidylethanolamine—0.0647—pH 7.4, 37 °CPMID 16527816Open