CatLogenzyme kinetics catalog

Values CatLog read from this paper

Functional analysis of the purified anandamide-generating phospholipase D as a member of the metallo-beta-lactamase family

PMID 16527816doi:10.1074/jbc.m512359200Open in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 20 records (20 values) from this paper: NAPE-PLD (N-acylphosphatidylethanolamine-hydrolyzing phospholipase D) from Rattus norvegicus, Homo sapiens, Mus musculus.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.

EC 3.1.4.54

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)Conditions
NAPE-PLD (N-acylphosphatidylethanolamine-hydrolyzing phospholipase D)Homo sapiensN-palmitoyl-1,2-dioleoylphosphatidylethanolamine—0.0046—pH 7.4, 37 °COpen
NAPE-PLD (N-acylphosphatidylethanolamine-hydrolyzing phospholipase D)variant D389NHomo sapiensN-palmitoyl-1,2-dioleoylphosphatidylethanolamine—0.0029—pH 7.4, 37 °COpen
NAPE-PLD (N-acylphosphatidylethanolamine-hydrolyzing phospholipase D)variant S152AHomo sapiensN-palmitoyl-1,2-dioleoylphosphatidylethanolamine—0.0037—pH 7.4, 37 °COpen
NAPE-PLD (N-acylphosphatidylethanolamine-hydrolyzing phospholipase D)Mus musculusN-palmitoyl-1,2-dioleoylphosphatidylethanolamine—0.0045—pH 7.4, 37 °COpen
NAPE-PLD (N-acylphosphatidylethanolamine-hydrolyzing phospholipase D)variant 387stopMus musculusN-palmitoyl-1,2-dioleoylphosphatidylethanolamine—0.0036—pH 7.4, 37 °COpen
NAPE-PLD (N-acylphosphatidylethanolamine-hydrolyzing phospholipase D)variant ΔN55Mus musculusN-palmitoyl-1,2-dioleoylphosphatidylethanolamine—0.0029—pH 7.4, 37 °COpen
NAPE-PLD (N-acylphosphatidylethanolamine-hydrolyzing phospholipase D)Rattus norvegicusN-palmitoyl-1,2-dioleoylphosphatidylethanolaminepurified recombinant enzyme (3.1 ng); 10 mM MgCl2; purified—0.0017—pH 7.4, 37 °COpen
NAPE-PLD (N-acylphosphatidylethanolamine-hydrolyzing phospholipase D)Rattus norvegicusN-palmitoyl-1,2-dioleoylphosphatidylethanolaminepurified recombinant enzyme (31 ng); 5 mM EDTA (Mg2+-free)—0.0059—pH 7.4, 37 °COpen
NAPE-PLD (N-acylphosphatidylethanolamine-hydrolyzing phospholipase D)Rattus norvegicusN-palmitoyl-1,2-dioleoylphosphatidylethanolamine10 mM MgCl2; expressed in COS-7 cells (pcDNA3.1(+))—0.0047—pH 7.4, 37 °COpen
NAPE-PLD (N-acylphosphatidylethanolamine-hydrolyzing phospholipase D)variant C170SRattus norvegicusN-palmitoyl-1,2-dioleoylphosphatidylethanolamine—0.0047—pH 7.4, 37 °COpen
NAPE-PLD (N-acylphosphatidylethanolamine-hydrolyzing phospholipase D)variant C222SRattus norvegicusN-palmitoyl-1,2-dioleoylphosphatidylethanolamine—0.0043—pH 7.4, 37 °COpen
NAPE-PLD (N-acylphosphatidylethanolamine-hydrolyzing phospholipase D)variant C224SRattus norvegicusN-palmitoyl-1,2-dioleoylphosphatidylethanolamine—0.0143—pH 7.4, 37 °COpen
NAPE-PLD (N-acylphosphatidylethanolamine-hydrolyzing phospholipase D)variant C237SRattus norvegicusN-palmitoyl-1,2-dioleoylphosphatidylethanolamine—0.0042—pH 7.4, 37 °COpen
NAPE-PLD (N-acylphosphatidylethanolamine-hydrolyzing phospholipase D)variant C255SRattus norvegicusN-palmitoyl-1,2-dioleoylphosphatidylethanolamine—0.0061—pH 7.4, 37 °COpen
NAPE-PLD (N-acylphosphatidylethanolamine-hydrolyzing phospholipase D)variant C288SRattus norvegicusN-palmitoyl-1,2-dioleoylphosphatidylethanolamine—0.0045—pH 7.4, 37 °COpen
NAPE-PLD (N-acylphosphatidylethanolamine-hydrolyzing phospholipase D)variant H331NRattus norvegicusN-palmitoyl-1,2-dioleoylphosphatidylethanolamine—0.0211—pH 7.4, 37 °COpen
NAPE-PLD (N-acylphosphatidylethanolamine-hydrolyzing phospholipase D)variant H343NRattus norvegicusN-palmitoyl-1,2-dioleoylphosphatidylethanolamine—0.0039—pH 7.4, 37 °COpen
NAPE-PLD (N-acylphosphatidylethanolamine-hydrolyzing phospholipase D)variant H353NRattus norvegicusN-palmitoyl-1,2-dioleoylphosphatidylethanolamine—0.0055—pH 7.4, 37 °COpen
NAPE-PLD (N-acylphosphatidylethanolamine-hydrolyzing phospholipase D)variant H380RRattus norvegicusN-palmitoyl-1,2-dioleoylphosphatidylethanolamine—0.0142—pH 7.4, 37 °COpen
NAPE-PLD (N-acylphosphatidylethanolamine-hydrolyzing phospholipase D)variant L207FRattus norvegicusN-palmitoyl-1,2-dioleoylphosphatidylethanolamine—0.0647—pH 7.4, 37 °COpen