EC 3.2.1.103: keratan-sulfate endo-1,4-beta-galactosidase
HydrolasesOpen in Browse
CatLog read 9 records for EC 3.2.1.103 from 1 paper, for 2 organisms. Names in these papers: endo-β-galactosidase.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units.
CatLog also holds 2 records for EC 3.2.1.103 that are not yet re-read: from its earlier version, built from the enzyme databases and partly checked against the papers. All records for EC 3.2.1.103 in Browse.
Papers
| Paper | Organisms | Records |
|---|---|---|
| Kinetic studies on endo-beta-galactosidase by a novel colorimetric assay and synthesis of N-acetyllactosamine-repeating oligosaccharide beta-glycosides using its transglycosylation activity | Bacteroides fragilis, Escherichia freundii | 9 |
Records
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Paper | Open record |
|---|---|---|---|---|---|---|---|---|
| endo-β-galactosidase | Bacteroides fragilis | 4-nitrophenyl 2-acetamido-2-deoxy-beta-D-glucopyranosyl-(1->3)-beta-D-galactopyranosyl-(1->4)-2-acetamido-2-deoxy-beta-D-glucopyranoside | 6.1 | 0.46 | 13.3 | pH 5.8, 37 °C | PMID 12950254 | Open record: endo-β-galactosidase, 4-nitrophenyl 2-acetamido-2-deoxy-beta-D-glucopyranosyl-(1->3)-beta-D-galactopyranosyl-(1->4)-2-acetamido-2-deoxy-beta-D-glucopyranoside |
| endo-β-galactosidase | Bacteroides fragilis | 4-nitrophenyl 2-acetamido-2-deoxy-beta-D-glucopyranosyl-(1->3)-beta-D-galactopyranosyl-(1->4)-beta-D-glucopyranoside | 0.21 | 2.16 | 0.1 | pH 5.8, 37 °C | PMID 12950254 | Open record: endo-β-galactosidase, 4-nitrophenyl 2-acetamido-2-deoxy-beta-D-glucopyranosyl-(1->3)-beta-D-galactopyranosyl-(1->4)-beta-D-glucopyranoside |
| endo-β-galactosidase | Bacteroides fragilis | 4-nitrophenyl beta-D-galactopyranosyl-(1->4)-2-acetamido-2-deoxy-beta-D-glucopyranosyl-(1->3)-beta-D-galactopyranosyl-(1->4)-2-acetamido-2-deoxy-beta-D-glucopyranoside | 5.59 | 0.13 | 43 | pH 5.8, 37 °C | PMID 12950254 | Open record: endo-β-galactosidase, 4-nitrophenyl beta-D-galactopyranosyl-(1->4)-2-acetamido-2-deoxy-beta-D-glucopyranosyl-(1->3)-beta-D-galactopyranosyl-(1->4)-2-acetamido-2-deoxy-beta-D-glucopyranoside |
| endo-β-galactosidase | Bacteroides fragilis | 4-nitrophenyl beta-D-galactopyranosyl-(1->4)-2-acetamido-2-deoxy-beta-D-glucopyranosyl-(1->3)-beta-D-galactopyranosyl-(1->4)-beta-D-glucopyranoside | 2.93 | 2.75 | 1.07 | pH 5.8, 37 °C | PMID 12950254 | Open record: endo-β-galactosidase, 4-nitrophenyl beta-D-galactopyranosyl-(1->4)-2-acetamido-2-deoxy-beta-D-glucopyranosyl-(1->3)-beta-D-galactopyranosyl-(1->4)-beta-D-glucopyranoside |
| endo-β-galactosidase | Escherichia freundii | 4-nitrophenyl 2-acetamido-2-deoxy-beta-D-glucopyranosyl-(1->3)-beta-D-galactopyranosyl-(1->4)-2-acetamido-2-deoxy-beta-D-glucopyranoside | 19.7 | 0.07 | 281 | pH 5.8, 37 °C | PMID 12950254 | Open record: endo-β-galactosidase, 4-nitrophenyl 2-acetamido-2-deoxy-beta-D-glucopyranosyl-(1->3)-beta-D-galactopyranosyl-(1->4)-2-acetamido-2-deoxy-beta-D-glucopyranoside |
| endo-β-galactosidase | Escherichia freundii | 4-nitrophenyl 2-acetamido-2-deoxy-beta-D-glucopyranosyl-(1->3)-beta-D-galactopyranosyl-(1->4)-beta-D-glucopyranoside | 0.86 | 0.87 | 0.46 | pH 5.8, 37 °C | PMID 12950254 | Open record: endo-β-galactosidase, 4-nitrophenyl 2-acetamido-2-deoxy-beta-D-glucopyranosyl-(1->3)-beta-D-galactopyranosyl-(1->4)-beta-D-glucopyranoside |
| endo-β-galactosidase | Escherichia freundii | 4-nitrophenyl beta-D-galactopyranosyl-(1->3)-2-acetamido-2-deoxy-beta-D-glucopyranosyl-(1->3)-beta-D-galactopyranosyl-(1->4)-beta-D-glucopyranoside | 1.64 | 1.42 | 1.15 | pH 5.8, 37 °C | PMID 12950254 | Open record: endo-β-galactosidase, 4-nitrophenyl beta-D-galactopyranosyl-(1->3)-2-acetamido-2-deoxy-beta-D-glucopyranosyl-(1->3)-beta-D-galactopyranosyl-(1->4)-beta-D-glucopyranoside |
| endo-β-galactosidase | Escherichia freundii | 4-nitrophenyl beta-D-galactopyranosyl-(1->4)-2-acetamido-2-deoxy-beta-D-glucopyranosyl-(1->3)-beta-D-galactopyranosyl-(1->4)-2-acetamido-2-deoxy-beta-D-glucopyranoside | 72.9 | 0.08 | 911 | pH 5.8, 37 °C | PMID 12950254 | Open record: endo-β-galactosidase, 4-nitrophenyl beta-D-galactopyranosyl-(1->4)-2-acetamido-2-deoxy-beta-D-glucopyranosyl-(1->3)-beta-D-galactopyranosyl-(1->4)-2-acetamido-2-deoxy-beta-D-glucopyranoside |
| endo-β-galactosidase | Escherichia freundii | 4-nitrophenyl beta-D-galactopyranosyl-(1->4)-2-acetamido-2-deoxy-beta-D-glucopyranosyl-(1->3)-beta-D-galactopyranosyl-(1->4)-beta-D-glucopyranoside | 18 | 2.1 | 8.6 | pH 5.8, 37 °C | PMID 12950254 | Open record: endo-β-galactosidase, 4-nitrophenyl beta-D-galactopyranosyl-(1->4)-2-acetamido-2-deoxy-beta-D-glucopyranosyl-(1->3)-beta-D-galactopyranosyl-(1->4)-beta-D-glucopyranoside |