Values CatLog read from this paper
Kinetic studies on endo-beta-galactosidase by a novel colorimetric assay and synthesis of N-acetyllactosamine-repeating oligosaccharide beta-glycosides using its transglycosylation activity
PMID 12950254doi:10.1046/j.1432-1033.2003.03757.xOpen in Browse
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CatLog read 9 records (27 values) from this paper: endo-β-galactosidase from Escherichia freundii, Bacteroides fragilis.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| endo-β-galactosidase | Bacteroides fragilis | 4-nitrophenyl 2-acetamido-2-deoxy-beta-D-glucopyranosyl-(1->3)-beta-D-galactopyranosyl-(1->4)-2-acetamido-2-deoxy-beta-D-glucopyranoside | 6.1 | 0.46 | 13.3 | pH 5.8, 37 °C | Open record: endo-β-galactosidase, 4-nitrophenyl 2-acetamido-2-deoxy-beta-D-glucopyranosyl-(1->3)-beta-D-galactopyranosyl-(1->4)-2-acetamido-2-deoxy-beta-D-glucopyranoside |
| endo-β-galactosidase | Bacteroides fragilis | 4-nitrophenyl 2-acetamido-2-deoxy-beta-D-glucopyranosyl-(1->3)-beta-D-galactopyranosyl-(1->4)-beta-D-glucopyranoside | 0.21 | 2.16 | 0.1 | pH 5.8, 37 °C | Open record: endo-β-galactosidase, 4-nitrophenyl 2-acetamido-2-deoxy-beta-D-glucopyranosyl-(1->3)-beta-D-galactopyranosyl-(1->4)-beta-D-glucopyranoside |
| endo-β-galactosidase | Bacteroides fragilis | 4-nitrophenyl beta-D-galactopyranosyl-(1->4)-2-acetamido-2-deoxy-beta-D-glucopyranosyl-(1->3)-beta-D-galactopyranosyl-(1->4)-2-acetamido-2-deoxy-beta-D-glucopyranoside | 5.59 | 0.13 | 43 | pH 5.8, 37 °C | Open record: endo-β-galactosidase, 4-nitrophenyl beta-D-galactopyranosyl-(1->4)-2-acetamido-2-deoxy-beta-D-glucopyranosyl-(1->3)-beta-D-galactopyranosyl-(1->4)-2-acetamido-2-deoxy-beta-D-glucopyranoside |
| endo-β-galactosidase | Bacteroides fragilis | 4-nitrophenyl beta-D-galactopyranosyl-(1->4)-2-acetamido-2-deoxy-beta-D-glucopyranosyl-(1->3)-beta-D-galactopyranosyl-(1->4)-beta-D-glucopyranoside | 2.93 | 2.75 | 1.07 | pH 5.8, 37 °C | Open record: endo-β-galactosidase, 4-nitrophenyl beta-D-galactopyranosyl-(1->4)-2-acetamido-2-deoxy-beta-D-glucopyranosyl-(1->3)-beta-D-galactopyranosyl-(1->4)-beta-D-glucopyranoside |
| endo-β-galactosidase | Escherichia freundii | 4-nitrophenyl 2-acetamido-2-deoxy-beta-D-glucopyranosyl-(1->3)-beta-D-galactopyranosyl-(1->4)-2-acetamido-2-deoxy-beta-D-glucopyranoside | 19.7 | 0.07 | 281 | pH 5.8, 37 °C | Open record: endo-β-galactosidase, 4-nitrophenyl 2-acetamido-2-deoxy-beta-D-glucopyranosyl-(1->3)-beta-D-galactopyranosyl-(1->4)-2-acetamido-2-deoxy-beta-D-glucopyranoside |
| endo-β-galactosidase | Escherichia freundii | 4-nitrophenyl 2-acetamido-2-deoxy-beta-D-glucopyranosyl-(1->3)-beta-D-galactopyranosyl-(1->4)-beta-D-glucopyranoside | 0.86 | 0.87 | 0.46 | pH 5.8, 37 °C | Open record: endo-β-galactosidase, 4-nitrophenyl 2-acetamido-2-deoxy-beta-D-glucopyranosyl-(1->3)-beta-D-galactopyranosyl-(1->4)-beta-D-glucopyranoside |
| endo-β-galactosidase | Escherichia freundii | 4-nitrophenyl beta-D-galactopyranosyl-(1->3)-2-acetamido-2-deoxy-beta-D-glucopyranosyl-(1->3)-beta-D-galactopyranosyl-(1->4)-beta-D-glucopyranoside | 1.64 | 1.42 | 1.15 | pH 5.8, 37 °C | Open record: endo-β-galactosidase, 4-nitrophenyl beta-D-galactopyranosyl-(1->3)-2-acetamido-2-deoxy-beta-D-glucopyranosyl-(1->3)-beta-D-galactopyranosyl-(1->4)-beta-D-glucopyranoside |
| endo-β-galactosidase | Escherichia freundii | 4-nitrophenyl beta-D-galactopyranosyl-(1->4)-2-acetamido-2-deoxy-beta-D-glucopyranosyl-(1->3)-beta-D-galactopyranosyl-(1->4)-2-acetamido-2-deoxy-beta-D-glucopyranoside | 72.9 | 0.08 | 911 | pH 5.8, 37 °C | Open record: endo-β-galactosidase, 4-nitrophenyl beta-D-galactopyranosyl-(1->4)-2-acetamido-2-deoxy-beta-D-glucopyranosyl-(1->3)-beta-D-galactopyranosyl-(1->4)-2-acetamido-2-deoxy-beta-D-glucopyranoside |
| endo-β-galactosidase | Escherichia freundii | 4-nitrophenyl beta-D-galactopyranosyl-(1->4)-2-acetamido-2-deoxy-beta-D-glucopyranosyl-(1->3)-beta-D-galactopyranosyl-(1->4)-beta-D-glucopyranoside | 18 | 2.1 | 8.6 | pH 5.8, 37 °C | Open record: endo-β-galactosidase, 4-nitrophenyl beta-D-galactopyranosyl-(1->4)-2-acetamido-2-deoxy-beta-D-glucopyranosyl-(1->3)-beta-D-galactopyranosyl-(1->4)-beta-D-glucopyranoside |