EC 3.2.1.133: glucan 1,4-alpha-maltohydrolase
HydrolasesOpen in Browse
CatLog read 47 records for EC 3.2.1.133 from 4 papers, for 4 organisms. Names in these papers: maltogenic amylase (Gt-Mamy), maltogenic amylase (ThMA), maltogenic amylase, maltose-forming α-amylase (PSMA).
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units.
CatLog also holds 15 records for EC 3.2.1.133 that are not yet re-read: from its earlier version, built from the enzyme databases and partly checked against the papers. All records for EC 3.2.1.133 in Browse.
Papers
| Paper | Organisms | Records |
|---|---|---|
| Dimerization mediates thermo-adaptation, substrate affinity and transglycosylation in a highly thermostable maltogenic amylase of Geobacillus thermoleovorans | Geobacillus thermoleovorans | 25 |
| Mutagenesis of Ala290, which modulates substrate subsite affinity at the catalytic interface of dimeric ThMA | Thermus sp. | 12 |
| Enhanced maltose production through mutagenesis of acceptor binding subsite +2 in Bacillus stearothermophilus maltogenic amylase | Bacillus stearothermophilus | 6 |
| Maltose-forming α-amylase from the hyperthermophilic archaeon Pyrococcus sp. ST04 | Pyrococcus sp. ST04 | 4 |
Records
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Paper | Open record |
|---|---|---|---|---|---|---|---|---|
| maltogenic amylase | Bacillus stearothermophilus | maltotriose | 137.2 | 12.3 | 11.15 | pH 5.5, 60 °C | PMID 26597712 | Open record: maltogenic amylase, maltotriose |
| maltogenic amylasevariant W177F | Bacillus stearothermophilus | maltotriose | 70.97 | 16.4 | 4.355 | pH 5.5, 60 °C | PMID 26597712 | Open record: maltogenic amylase variant W177F, maltotriose |
| maltogenic amylasevariant W177L | Bacillus stearothermophilus | maltotriose | 126.2 | 28 | 4.507 | pH 5.5, 60 °C | PMID 26597712 | Open record: maltogenic amylase variant W177L, maltotriose |
| maltogenic amylasevariant W177N | Bacillus stearothermophilus | maltotriose | 151.0 | 31.7 | 4.763 | pH 5.5, 60 °C | PMID 26597712 | Open record: maltogenic amylase variant W177N, maltotriose |
| maltogenic amylasevariant W177S | Bacillus stearothermophilus | maltotriose | 182.8 | 35.3 | 5.178 | pH 5.5, 60 °C | PMID 26597712 | Open record: maltogenic amylase variant W177S, maltotriose |
| maltogenic amylasevariant W177Y | Bacillus stearothermophilus | maltotriose | 108.3 | 22 | 4.92 | pH 5.5, 60 °C | PMID 26597712 | Open record: maltogenic amylase variant W177Y, maltotriose |
| maltogenic amylase (Gt-Mamy) | Geobacillus thermoleovorans | alpha-cyclodextrin (cyclomaltohexaose)monomeric form (72.5 kDa) | 0.1007 | — | — | pH 7, 80 °C | PMID 24069213 | Open record: maltogenic amylase (Gt-Mamy), alpha-cyclodextrin (cyclomaltohexaose) |
| maltogenic amylase (Gt-Mamy) | Geobacillus thermoleovorans | alpha-cyclodextrin (cyclomaltohexaose)dimeric form (145 kDa) | 2.78 | — | — | pH 7, 80 °C | PMID 24069213 | Open record: maltogenic amylase (Gt-Mamy), alpha-cyclodextrin (cyclomaltohexaose) |
| maltogenic amylase (Gt-Mamy) | Geobacillus thermoleovorans | Amylopectinmonomeric form (72.5 kDa) | 0.172 | — | — | pH 7, 80 °C | PMID 24069213 | Open record: maltogenic amylase (Gt-Mamy), Amylopectin |
| maltogenic amylase (Gt-Mamy) | Geobacillus thermoleovorans | Amylopectindimeric form (145 kDa) | 0.038 | — | — | pH 7, 80 °C | PMID 24069213 | Open record: maltogenic amylase (Gt-Mamy), Amylopectin |
| maltogenic amylase (Gt-Mamy) | Geobacillus thermoleovorans | Amylosemonomeric form (72.5 kDa) | 0.212 | — | — | pH 7, 80 °C | PMID 24069213 | Open record: maltogenic amylase (Gt-Mamy), Amylose |
| maltogenic amylase (Gt-Mamy) | Geobacillus thermoleovorans | Amylosedimeric form (145 kDa) | 0.043 | — | — | pH 7, 80 °C | PMID 24069213 | Open record: maltogenic amylase (Gt-Mamy), Amylose |
| maltogenic amylase (Gt-Mamy) | Geobacillus thermoleovorans | beta-cyclodextrin (cyclomaltoheptaose)monomeric form (72.5 kDa) | 0.1227 | — | — | pH 7, 80 °C | PMID 24069213 | Open record: maltogenic amylase (Gt-Mamy), beta-cyclodextrin (cyclomaltoheptaose) |
| maltogenic amylase (Gt-Mamy) | Geobacillus thermoleovorans | beta-cyclodextrin (cyclomaltoheptaose)dimeric form (145 kDa) | 1.325 | — | — | pH 7, 80 °C | PMID 24069213 | Open record: maltogenic amylase (Gt-Mamy), beta-cyclodextrin (cyclomaltoheptaose) |
| maltogenic amylase (Gt-Mamy) | Geobacillus thermoleovorans | Soluble starchmonomeric form (72.5 kDa) | 0.202 | — | — | pH 7, 80 °C | PMID 24069213 | Open record: maltogenic amylase (Gt-Mamy), Soluble starch |
| maltogenic amylase (Gt-Mamy) | Geobacillus thermoleovorans | Soluble starchdimeric form (145 kDa) | 0.038 | — | — | pH 7, 80 °C | PMID 24069213 | Open record: maltogenic amylase (Gt-Mamy), Soluble starch |
| maltogenic amylase (Gt-Mamy)variant D109A | Geobacillus thermoleovorans | alpha-cyclodextrin (cyclomaltohexaose) | 0.7548 | — | — | pH 7, 80 °C | PMID 24069213 | Open record: maltogenic amylase (Gt-Mamy) variant D109A, alpha-cyclodextrin (cyclomaltohexaose) |
| maltogenic amylase (Gt-Mamy)variant D109A | Geobacillus thermoleovorans | Amylopectin | 0.1027 | — | — | pH 7, 80 °C | PMID 24069213 | Open record: maltogenic amylase (Gt-Mamy) variant D109A, Amylopectin |
| maltogenic amylase (Gt-Mamy)variant D109A | Geobacillus thermoleovorans | Amylose | 0.1303 | — | — | pH 7, 80 °C | PMID 24069213 | Open record: maltogenic amylase (Gt-Mamy) variant D109A, Amylose |
| maltogenic amylase (Gt-Mamy)variant D109A | Geobacillus thermoleovorans | beta-cyclodextrin (cyclomaltoheptaose) | 0.4887 | — | — | pH 7, 80 °C | PMID 24069213 | Open record: maltogenic amylase (Gt-Mamy) variant D109A, beta-cyclodextrin (cyclomaltoheptaose) |
| maltogenic amylase (Gt-Mamy)variant D109A | Geobacillus thermoleovorans | Soluble starch | 0.123 | — | — | pH 7, 80 °C | PMID 24069213 | Open record: maltogenic amylase (Gt-Mamy) variant D109A, Soluble starch |
| maltogenic amylase (Gt-Mamy)variant D109E | Geobacillus thermoleovorans | alpha-cyclodextrin (cyclomaltohexaose) | 1.389 | — | — | pH 7, 80 °C | PMID 24069213 | Open record: maltogenic amylase (Gt-Mamy) variant D109E, alpha-cyclodextrin (cyclomaltohexaose) |
| maltogenic amylase (Gt-Mamy)variant D109E | Geobacillus thermoleovorans | Amylopectin | 0.0048 | — | — | pH 7, 80 °C | PMID 24069213 | Open record: maltogenic amylase (Gt-Mamy) variant D109E, Amylopectin |
| maltogenic amylase (Gt-Mamy)variant D109E | Geobacillus thermoleovorans | Amylose | 0.0223 | — | — | pH 7, 80 °C | PMID 24069213 | Open record: maltogenic amylase (Gt-Mamy) variant D109E, Amylose |
| maltogenic amylase (Gt-Mamy)variant D109E | Geobacillus thermoleovorans | beta-cyclodextrin (cyclomaltoheptaose) | 0.6648 | — | — | pH 7, 80 °C | PMID 24069213 | Open record: maltogenic amylase (Gt-Mamy) variant D109E, beta-cyclodextrin (cyclomaltoheptaose) |
| maltogenic amylase (Gt-Mamy)variant D109E | Geobacillus thermoleovorans | Soluble starch | 0.021 | — | — | pH 7, 80 °C | PMID 24069213 | Open record: maltogenic amylase (Gt-Mamy) variant D109E, Soluble starch |
| maltogenic amylase (Gt-Mamy)variant Δ128 | Geobacillus thermoleovorans | alpha-cyclodextrin (cyclomaltohexaose) | 0.0962 | — | — | pH 7, 80 °C | PMID 24069213 | Open record: maltogenic amylase (Gt-Mamy) variant Δ128, alpha-cyclodextrin (cyclomaltohexaose) |
| maltogenic amylase (Gt-Mamy)variant Δ128 | Geobacillus thermoleovorans | Amylopectin | 0.047 | — | — | pH 7, 80 °C | PMID 24069213 | Open record: maltogenic amylase (Gt-Mamy) variant Δ128, Amylopectin |
| maltogenic amylase (Gt-Mamy)variant Δ128 | Geobacillus thermoleovorans | Amylose | 0.208 | — | — | pH 7, 80 °C | PMID 24069213 | Open record: maltogenic amylase (Gt-Mamy) variant Δ128, Amylose |
| maltogenic amylase (Gt-Mamy)variant Δ128 | Geobacillus thermoleovorans | beta-cyclodextrin (cyclomaltoheptaose) | 0.1203 | — | — | pH 7, 80 °C | PMID 24069213 | Open record: maltogenic amylase (Gt-Mamy) variant Δ128, beta-cyclodextrin (cyclomaltoheptaose) |
| maltogenic amylase (Gt-Mamy)variant Δ128 | Geobacillus thermoleovorans | Soluble starch | 0.197 | — | — | pH 7, 80 °C | PMID 24069213 | Open record: maltogenic amylase (Gt-Mamy) variant Δ128, Soluble starch |
| maltogenic amylase (ThMA) | Thermus sp. | Maltoheptaose | 384.1 | 9.21 | 41.72 | pH 6, 50 °C | PMID 15975859 | Open record: maltogenic amylase (ThMA), Maltoheptaose |
| maltogenic amylase (ThMA) | Thermus sp. | Maltohexaose | 160.3 | 7.16 | 22.38 | pH 6, 50 °C | PMID 15975859 | Open record: maltogenic amylase (ThMA), Maltohexaose |
| maltogenic amylase (ThMA) | Thermus sp. | Maltopentaose | 197.0 | 3.97 | 49.6 | pH 6, 50 °C | PMID 15975859 | Open record: maltogenic amylase (ThMA), Maltopentaose |
| maltogenic amylase (ThMA) | Thermus sp. | Maltose | 0.29 | 63.43 | 0.0046 | pH 6, 50 °C | PMID 15975859 | Open record: maltogenic amylase (ThMA), Maltose |
| maltogenic amylase (ThMA) | Thermus sp. | Maltotetraose | 574.1 | 3.38 | 170.0 | pH 6, 50 °C | PMID 15975859 | Open record: maltogenic amylase (ThMA), Maltotetraose |
| maltogenic amylase (ThMA) | Thermus sp. | Maltotriose | 652.5 | 1.13 | 579.4 | pH 6, 50 °C | PMID 15975859 | Open record: maltogenic amylase (ThMA), Maltotriose |
| maltogenic amylase (ThMA)variant A290I | Thermus sp. | Maltoheptaose | 72.08 | 8.17 | 8.82 | pH 6, 50 °C | PMID 15975859 | Open record: maltogenic amylase (ThMA) variant A290I, Maltoheptaose |
| maltogenic amylase (ThMA)variant A290I | Thermus sp. | Maltohexaose | 88.45 | 7.53 | 11.74 | pH 6, 50 °C | PMID 15975859 | Open record: maltogenic amylase (ThMA) variant A290I, Maltohexaose |
| maltogenic amylase (ThMA)variant A290I | Thermus sp. | Maltopentaose | 69.01 | 3.5 | 19.7 | pH 6, 50 °C | PMID 15975859 | Open record: maltogenic amylase (ThMA) variant A290I, Maltopentaose |
| maltogenic amylase (ThMA)variant A290I | Thermus sp. | Maltose | 0.04 | 427 | 9.6 × 10⁻⁵ | pH 6, 50 °C | PMID 15975859 | Open record: maltogenic amylase (ThMA) variant A290I, Maltose |
| maltogenic amylase (ThMA)variant A290I | Thermus sp. | Maltotetraose | 173.5 | 2.95 | 58.86 | pH 6, 50 °C | PMID 15975859 | Open record: maltogenic amylase (ThMA) variant A290I, Maltotetraose |
| maltogenic amylase (ThMA)variant A290I | Thermus sp. | Maltotriose | 79.07 | 2.52 | 31.4 | pH 6, 50 °C | PMID 15975859 | Open record: maltogenic amylase (ThMA) variant A290I, Maltotriose |
| maltose-forming α-amylase (PSMA) | Pyrococcus sp. ST04 | 6-O-maltosyl-β-cyclodextrin | 25.75 | 0.3 | 85.8 | pH 5, 90 °C | PMID 23884203 | Open record: maltose-forming α-amylase (PSMA), 6-O-maltosyl-β-cyclodextrin |
| maltose-forming α-amylase (PSMA) | Pyrococcus sp. ST04 | Maltopentaose | 0.0017 | 21.2 | 7.8 × 10⁻⁵ | pH 5, 90 °C | PMID 23884203 | Open record: maltose-forming α-amylase (PSMA), Maltopentaose |
| maltose-forming α-amylase (PSMA) | Pyrococcus sp. ST04 | Maltotetraose | 0.0033 | 18.8 | 1.8 × 10⁻⁴ | pH 5, 90 °C | PMID 23884203 | Open record: maltose-forming α-amylase (PSMA), Maltotetraose |
| maltose-forming α-amylase (PSMA) | Pyrococcus sp. ST04 | Maltotriose | 6.35 | 8.4 | 0.757 | pH 5, 90 °C | PMID 23884203 | Open record: maltose-forming α-amylase (PSMA), Maltotriose |