EC 3.2.1.168: hesperidin 6-O-alpha-L-rhamnosyl-beta-D-glucosidase
HydrolasesOpen in Browse
CatLog read 6 records for EC 3.2.1.168 from 1 paper, for 2 organisms. Names in these papers: rutinosidase (AnRut), rutinosidase (AoRut).
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units.
CatLog also holds 2 records for EC 3.2.1.168 that are not yet re-read: from its earlier version, built from the enzyme databases and partly checked against the papers. All records for EC 3.2.1.168 in Browse.
Papers
| Paper | Organisms | Records |
|---|---|---|
| Fungal Rutinosidase─Engineering of a Side Tunnel Steers Its Transglycosylation Potential | Aspergillus niger, Aspergillus oryzae | 6 |
Records
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Paper | Open record |
|---|---|---|---|---|---|---|---|---|
| rutinosidase (AnRut) | Aspergillus niger | 4-nitrophenyl 6-O-(alpha-L-rhamnopyranosyl)-beta-D-glucopyranoside | 32 | 51 | 0.62 | pH 3.5, 36 °C | PMID 42130197 | Open record: rutinosidase (AnRut), 4-nitrophenyl 6-O-(alpha-L-rhamnopyranosyl)-beta-D-glucopyranoside |
| rutinosidase (AnRut)variant F221A (mut3) | Aspergillus niger | 4-nitrophenyl 6-O-(alpha-L-rhamnopyranosyl)-beta-D-glucopyranoside | 3.1 | 89 | 0.03 | pH 3.5, 36 °C | PMID 42130197 | Open record: rutinosidase (AnRut) variant F221A (mut3), 4-nitrophenyl 6-O-(alpha-L-rhamnopyranosyl)-beta-D-glucopyranoside |
| rutinosidase (AnRut)variant F221A/Y286A (mut4) | Aspergillus niger | 4-nitrophenyl 6-O-(alpha-L-rhamnopyranosyl)-beta-D-glucopyranoside | 3 | 61 | 0.05 | pH 3.5, 36 °C | PMID 42130197 | Open record: rutinosidase (AnRut) variant F221A/Y286A (mut4), 4-nitrophenyl 6-O-(alpha-L-rhamnopyranosyl)-beta-D-glucopyranoside |
| rutinosidase (AnRut)variant H288–/E287G/M289A (mut5) | Aspergillus niger | 4-nitrophenyl 6-O-(alpha-L-rhamnopyranosyl)-beta-D-glucopyranoside | 5.8 | 29 | 0.2 | pH 3.5, 36 °C | PMID 42130197 | Open record: rutinosidase (AnRut) variant H288–/E287G/M289A (mut5), 4-nitrophenyl 6-O-(alpha-L-rhamnopyranosyl)-beta-D-glucopyranoside |
| rutinosidase (AnRut)variant M218G/V219G/H288G (mut6) | Aspergillus niger | 4-nitrophenyl 6-O-(alpha-L-rhamnopyranosyl)-beta-D-glucopyranoside | 19.7 | 30 | 0.65 | pH 3.5, 36 °C | PMID 42130197 | Open record: rutinosidase (AnRut) variant M218G/V219G/H288G (mut6), 4-nitrophenyl 6-O-(alpha-L-rhamnopyranosyl)-beta-D-glucopyranoside |
| rutinosidase (AoRut) | Aspergillus oryzae | 4-nitrophenyl 6-O-(alpha-L-rhamnopyranosyl)-beta-D-glucopyranoside | 10.4 | 7.9 | 1.32 | pH 3.5, 36 °C | PMID 42130197 | Open record: rutinosidase (AoRut), 4-nitrophenyl 6-O-(alpha-L-rhamnopyranosyl)-beta-D-glucopyranoside |