Values CatLog read from this paper
Fungal Rutinosidase─Engineering of a Side Tunnel Steers Its Transglycosylation Potential
PMID 42130197doi:10.1021/acs.jafc.6c02816Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 6 records (18 values) from this paper: rutinosidase (AnRut), rutinosidase (AoRut) from Aspergillus niger, Aspergillus oryzae.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| rutinosidase (AnRut) | Aspergillus niger | 4-nitrophenyl 6-O-(alpha-L-rhamnopyranosyl)-beta-D-glucopyranoside | 32 | 51 | 0.62 | pH 3.5, 36 °C | Open record: rutinosidase (AnRut), 4-nitrophenyl 6-O-(alpha-L-rhamnopyranosyl)-beta-D-glucopyranoside |
| rutinosidase (AnRut)variant F221A (mut3) | Aspergillus niger | 4-nitrophenyl 6-O-(alpha-L-rhamnopyranosyl)-beta-D-glucopyranoside | 3.1 | 89 | 0.03 | pH 3.5, 36 °C | Open record: rutinosidase (AnRut) variant F221A (mut3), 4-nitrophenyl 6-O-(alpha-L-rhamnopyranosyl)-beta-D-glucopyranoside |
| rutinosidase (AnRut)variant F221A/Y286A (mut4) | Aspergillus niger | 4-nitrophenyl 6-O-(alpha-L-rhamnopyranosyl)-beta-D-glucopyranoside | 3 | 61 | 0.05 | pH 3.5, 36 °C | Open record: rutinosidase (AnRut) variant F221A/Y286A (mut4), 4-nitrophenyl 6-O-(alpha-L-rhamnopyranosyl)-beta-D-glucopyranoside |
| rutinosidase (AnRut)variant H288–/E287G/M289A (mut5) | Aspergillus niger | 4-nitrophenyl 6-O-(alpha-L-rhamnopyranosyl)-beta-D-glucopyranoside | 5.8 | 29 | 0.2 | pH 3.5, 36 °C | Open record: rutinosidase (AnRut) variant H288–/E287G/M289A (mut5), 4-nitrophenyl 6-O-(alpha-L-rhamnopyranosyl)-beta-D-glucopyranoside |
| rutinosidase (AnRut)variant M218G/V219G/H288G (mut6) | Aspergillus niger | 4-nitrophenyl 6-O-(alpha-L-rhamnopyranosyl)-beta-D-glucopyranoside | 19.7 | 30 | 0.65 | pH 3.5, 36 °C | Open record: rutinosidase (AnRut) variant M218G/V219G/H288G (mut6), 4-nitrophenyl 6-O-(alpha-L-rhamnopyranosyl)-beta-D-glucopyranoside |
| rutinosidase (AoRut) | Aspergillus oryzae | 4-nitrophenyl 6-O-(alpha-L-rhamnopyranosyl)-beta-D-glucopyranoside | 10.4 | 7.9 | 1.32 | pH 3.5, 36 °C | Open record: rutinosidase (AoRut), 4-nitrophenyl 6-O-(alpha-L-rhamnopyranosyl)-beta-D-glucopyranoside |