CatLogenzyme kinetics catalog

EC 3.2.1.73: licheninase

HydrolasesOpen in Browse

CatLog read 37 records for EC 3.2.1.73 from 2 papers, for 2 organisms. Names in these papers: 1,3-1,4-β-glucanase, endo-1,3-1,4-beta-D-glucanase (lichenase).

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units.

CatLog also holds 118 records for EC 3.2.1.73 that are not yet re-read: from its earlier version, built from the enzyme databases and partly checked against the papers. All records for EC 3.2.1.73 in Browse.

Papers

PaperOrganismsRecords
Pre-steady-state kinetics of Bacillus licheniformis 1,3-1,4-beta-glucanase: evidence for a regulatory binding siteBacillus licheniformis20
Structural basis for the substrate specificity of a Bacillus 1,3-1,4-beta-glucanaseBacillus macerans17

Records

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)ConditionsPaper
1,3-1,4-β-glucanaseBacillus licheniformis2,4-dinitrophenyl 3-O-β-cellobiosyl-β-D-glucoside4000.7500pH 7.2, 30 °CPMID 12568655Open
1,3-1,4-β-glucanaseBacillus licheniformis2,4-dinitrophenyl 3-O-β-cellobiosyl-β-D-glucosidepre-steady-state initial rates v0 = kobs·ΔP + vss (eqn 5)——1.12 × 10³pH 7.2, 30 °CPMID 12568655Open
1,3-1,4-β-glucanaseBacillus licheniformis3,4-dinitrophenyl 3-O-β-cellobiosyl-β-D-glucoside6.5 mM citrate; 87 mM Na2HPO4 buffer; 0.1 mM CaCl2; I 0.157.80.711pH 7.2, 30 °CPMID 12568655Open
1,3-1,4-β-glucanaseBacillus licheniformis3,4-dinitrophenyl 3-O-β-cellobiosyl-β-D-glucoside6.5 mM citrate; 87 mM Na2HPO4 buffer; 0.1 mM CaCl2; I 0.15430.4100pH 7.2, 30 °CPMID 12568655Open
1,3-1,4-β-glucanaseBacillus licheniformis3,4-dinitrophenyl 3-O-β-cellobiosyl-β-D-glucosidepre-steady-state initial rates v0 = kobs·ΔP + vss (eqn 5)410.3135pH 6.23, 30 °CPMID 12568655Open
1,3-1,4-β-glucanaseBacillus licheniformis3,4-dinitrophenyl 3-O-β-cellobiosyl-β-D-glucoside——15pH 6.23, 30 °CPMID 12568655Open
1,3-1,4-β-glucanaseBacillus licheniformis3,4-dinitrophenyl 3-O-β-cellobiosyl-β-D-glucosidepre-steady-state initial rates v0 = kobs·ΔP + vss (eqn 5)450.3150pH 6.76, 30 °CPMID 12568655Open
1,3-1,4-β-glucanaseBacillus licheniformis3,4-dinitrophenyl 3-O-β-cellobiosyl-β-D-glucoside——17pH 6.76, 30 °CPMID 12568655Open
1,3-1,4-β-glucanaseBacillus licheniformis3,4-dinitrophenyl 3-O-β-cellobiosyl-β-D-glucosidepH series; vss calculated for absent substrate inhibition420.4110pH 7.2, 30 °CPMID 12568655Open
1,3-1,4-β-glucanaseBacillus licheniformis3,4-dinitrophenyl 3-O-β-cellobiosyl-β-D-glucosidepH series; Table 6——11.6pH 7.2, 30 °CPMID 12568655Open
1,3-1,4-β-glucanaseBacillus licheniformis3,4-dinitrophenyl 3-O-β-cellobiosyl-β-D-glucosidepre-steady-state initial rates v0 = kobs·ΔP + vss (eqn 5)180.445pH 7.8, 30 °CPMID 12568655Open
1,3-1,4-β-glucanaseBacillus licheniformis3,4-dinitrophenyl 3-O-β-cellobiosyl-β-D-glucoside——4pH 7.8, 30 °CPMID 12568655Open
1,3-1,4-β-glucanaseBacillus licheniformis3,4-dinitrophenyl 3-O-β-cellobiosyl-β-D-glucosidepre-steady-state initial rates v0 = kobs·ΔP + vss (eqn 5)——17pH 8.25, 30 °CPMID 12568655Open
1,3-1,4-β-glucanaseBacillus licheniformis3,4-dinitrophenyl 3-O-β-cellobiosyl-β-D-glucoside——2.08pH 8.25, 30 °CPMID 12568655Open
1,3-1,4-β-glucanaseBacillus licheniformis4-methylumbelliferyl 3-O-β-cellobiosyl-β-D-glucoside0.510.46pH 7.2, 30 °CPMID 12568655Open
1,3-1,4-β-glucanaseBacillus licheniformis4-methylumbelliferyl 3-O-β-cellobiosyl-β-D-glucosidepre-steady-state initial rates v0 = kobs·ΔP + vss (eqn 5)71.16.5pH 7.2, 30 °CPMID 12568655Open
1,3-1,4-β-glucanaseBacillus licheniformis4-methylumbelliferyl 3-O-β-cellotetraosyl-β-D-glucoside0.60.60.8pH 7.2, 30 °CPMID 12568655Open
1,3-1,4-β-glucanaseBacillus licheniformis4-methylumbelliferyl 3-O-β-cellotetraosyl-β-D-glucosidepre-steady-state initial rates v0 = kobs·ΔP + vss (eqn 5)3.30.546.1pH 7.2, 30 °CPMID 12568655Open
1,3-1,4-β-glucanaseBacillus licheniformis4-methylumbelliferyl 3-O-β-cellotriosyl-β-D-glucoside0.40.50.8pH 7.2, 30 °CPMID 12568655Open
1,3-1,4-β-glucanaseBacillus licheniformis4-methylumbelliferyl 3-O-β-cellotriosyl-β-D-glucosidepre-steady-state initial rates v0 = kobs·ΔP + vss (eqn 5)40.75.8pH 7.2, 30 °CPMID 12568655Open
endo-1,3-1,4-beta-D-glucanase (lichenase)Bacillus maceranslaminarin0.263——pH 7.2, 50 °CPMID 16483609Open
endo-1,3-1,4-beta-D-glucanase (lichenase)variant A98WBacillus maceranslaminarin0.375——pH 7.2, 50 °CPMID 16483609Open
endo-1,3-1,4-beta-D-glucanase (lichenase)variant E131QBacillus maceranslaminarin0.217——pH 7.2, 50 °CPMID 16483609Open
endo-1,3-1,4-beta-D-glucanase (lichenase)variant E63DBacillus maceranslaminarin0.0097——pH 7.2, 50 °CPMID 16483609Open
endo-1,3-1,4-beta-D-glucanase (lichenase)variant E63QBacillus maceranslaminarin0.0039——pH 7.2, 50 °CPMID 16483609Open
endo-1,3-1,4-beta-D-glucanase (lichenase)variant H99DBacillus maceranslaminarin0.198——pH 7.2, 50 °CPMID 16483609Open
endo-1,3-1,4-beta-D-glucanase (lichenase)variant H99RBacillus maceranslaminarin0.178——pH 7.2, 50 °CPMID 16483609Open
endo-1,3-1,4-beta-D-glucanase (lichenase)variant N26ABacillus maceranslaminarin0.045——pH 7.2, 50 °CPMID 16483609Open
endo-1,3-1,4-beta-D-glucanase (lichenase)variant R65ABacillus maceranslaminarin0.09——pH 7.2, 50 °CPMID 16483609Open
endo-1,3-1,4-beta-D-glucanase (lichenase)variant S90ABacillus maceranslaminarin0.056——pH 7.2, 50 °CPMID 16483609Open
endo-1,3-1,4-beta-D-glucanase (lichenase)variant W184YBacillus maceranslaminarin0.095——pH 7.2, 50 °CPMID 16483609Open
endo-1,3-1,4-beta-D-glucanase (lichenase)variant W192ABacillus maceranslaminarin0.021——pH 7.2, 50 °CPMID 16483609Open
endo-1,3-1,4-beta-D-glucanase (lichenase)variant Y123ABacillus maceranslaminarin0.021——pH 7.2, 50 °CPMID 16483609Open
endo-1,3-1,4-beta-D-glucanase (lichenase)variant Y123FBacillus maceranslaminarin0.65——pH 7.2, 50 °CPMID 16483609Open
endo-1,3-1,4-beta-D-glucanase (lichenase)variant Y24ABacillus maceranslaminarin0.07——pH 7.2, 50 °CPMID 16483609Open
endo-1,3-1,4-beta-D-glucanase (lichenase)variant Y24FBacillus maceranslaminarin0.171——pH 7.2, 50 °CPMID 16483609Open
endo-1,3-1,4-beta-D-glucanase (lichenase)variant Y24WBacillus maceranslaminarin0.5——pH 7.2, 50 °CPMID 16483609Open