Values CatLog read from this paper
Pre-steady-state kinetics of Bacillus licheniformis 1,3-1,4-beta-glucanase: evidence for a regulatory binding site
PMID 12568655doi:10.1042/bj20021504Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 20 records (46 values) from this paper: 1,3-1,4-β-glucanase from Bacillus licheniformis.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| 1,3-1,4-β-glucanase | Bacillus licheniformis | 2,4-dinitrophenyl 3-O-β-cellobiosyl-β-D-glucoside | 400 | 0.7 | 500 | pH 7.2, 30 °C | Open record: 1,3-1,4-β-glucanase, 2,4-dinitrophenyl 3-O-β-cellobiosyl-β-D-glucoside |
| 1,3-1,4-β-glucanase | Bacillus licheniformis | 2,4-dinitrophenyl 3-O-β-cellobiosyl-β-D-glucosidepre-steady-state initial rates v0 = kobs·ΔP + vss (eqn 5) | — | — | 1.12 × 10³ | pH 7.2, 30 °C | Open record: 1,3-1,4-β-glucanase, 2,4-dinitrophenyl 3-O-β-cellobiosyl-β-D-glucoside |
| 1,3-1,4-β-glucanase | Bacillus licheniformis | 3,4-dinitrophenyl 3-O-β-cellobiosyl-β-D-glucoside6.5 mM citrate; 87 mM Na2HPO4 buffer; 0.1 mM CaCl2; I 0.15 | 7.8 | 0.7 | 11 | pH 7.2, 30 °C | Open record: 1,3-1,4-β-glucanase, 3,4-dinitrophenyl 3-O-β-cellobiosyl-β-D-glucoside |
| 1,3-1,4-β-glucanase | Bacillus licheniformis | 3,4-dinitrophenyl 3-O-β-cellobiosyl-β-D-glucoside6.5 mM citrate; 87 mM Na2HPO4 buffer; 0.1 mM CaCl2; I 0.15 | 43 | 0.4 | 100 | pH 7.2, 30 °C | Open record: 1,3-1,4-β-glucanase, 3,4-dinitrophenyl 3-O-β-cellobiosyl-β-D-glucoside |
| 1,3-1,4-β-glucanase | Bacillus licheniformis | 3,4-dinitrophenyl 3-O-β-cellobiosyl-β-D-glucosidepre-steady-state initial rates v0 = kobs·ΔP + vss (eqn 5) | 41 | 0.3 | 135 | pH 6.23, 30 °C | Open record: 1,3-1,4-β-glucanase, 3,4-dinitrophenyl 3-O-β-cellobiosyl-β-D-glucoside |
| 1,3-1,4-β-glucanase | Bacillus licheniformis | 3,4-dinitrophenyl 3-O-β-cellobiosyl-β-D-glucoside | — | — | 15 | pH 6.23, 30 °C | Open record: 1,3-1,4-β-glucanase, 3,4-dinitrophenyl 3-O-β-cellobiosyl-β-D-glucoside |
| 1,3-1,4-β-glucanase | Bacillus licheniformis | 3,4-dinitrophenyl 3-O-β-cellobiosyl-β-D-glucosidepre-steady-state initial rates v0 = kobs·ΔP + vss (eqn 5) | 45 | 0.3 | 150 | pH 6.76, 30 °C | Open record: 1,3-1,4-β-glucanase, 3,4-dinitrophenyl 3-O-β-cellobiosyl-β-D-glucoside |
| 1,3-1,4-β-glucanase | Bacillus licheniformis | 3,4-dinitrophenyl 3-O-β-cellobiosyl-β-D-glucoside | — | — | 17 | pH 6.76, 30 °C | Open record: 1,3-1,4-β-glucanase, 3,4-dinitrophenyl 3-O-β-cellobiosyl-β-D-glucoside |
| 1,3-1,4-β-glucanase | Bacillus licheniformis | 3,4-dinitrophenyl 3-O-β-cellobiosyl-β-D-glucosidepH series; vss calculated for absent substrate inhibition | 42 | 0.4 | 110 | pH 7.2, 30 °C | Open record: 1,3-1,4-β-glucanase, 3,4-dinitrophenyl 3-O-β-cellobiosyl-β-D-glucoside |
| 1,3-1,4-β-glucanase | Bacillus licheniformis | 3,4-dinitrophenyl 3-O-β-cellobiosyl-β-D-glucosidepH series; Table 6 | — | — | 11.6 | pH 7.2, 30 °C | Open record: 1,3-1,4-β-glucanase, 3,4-dinitrophenyl 3-O-β-cellobiosyl-β-D-glucoside |
| 1,3-1,4-β-glucanase | Bacillus licheniformis | 3,4-dinitrophenyl 3-O-β-cellobiosyl-β-D-glucosidepre-steady-state initial rates v0 = kobs·ΔP + vss (eqn 5) | 18 | 0.4 | 45 | pH 7.8, 30 °C | Open record: 1,3-1,4-β-glucanase, 3,4-dinitrophenyl 3-O-β-cellobiosyl-β-D-glucoside |
| 1,3-1,4-β-glucanase | Bacillus licheniformis | 3,4-dinitrophenyl 3-O-β-cellobiosyl-β-D-glucoside | — | — | 4 | pH 7.8, 30 °C | Open record: 1,3-1,4-β-glucanase, 3,4-dinitrophenyl 3-O-β-cellobiosyl-β-D-glucoside |
| 1,3-1,4-β-glucanase | Bacillus licheniformis | 3,4-dinitrophenyl 3-O-β-cellobiosyl-β-D-glucosidepre-steady-state initial rates v0 = kobs·ΔP + vss (eqn 5) | — | — | 17 | pH 8.25, 30 °C | Open record: 1,3-1,4-β-glucanase, 3,4-dinitrophenyl 3-O-β-cellobiosyl-β-D-glucoside |
| 1,3-1,4-β-glucanase | Bacillus licheniformis | 3,4-dinitrophenyl 3-O-β-cellobiosyl-β-D-glucoside | — | — | 2.08 | pH 8.25, 30 °C | Open record: 1,3-1,4-β-glucanase, 3,4-dinitrophenyl 3-O-β-cellobiosyl-β-D-glucoside |
| 1,3-1,4-β-glucanase | Bacillus licheniformis | 4-methylumbelliferyl 3-O-β-cellobiosyl-β-D-glucoside | 0.5 | 1 | 0.46 | pH 7.2, 30 °C | Open record: 1,3-1,4-β-glucanase, 4-methylumbelliferyl 3-O-β-cellobiosyl-β-D-glucoside |
| 1,3-1,4-β-glucanase | Bacillus licheniformis | 4-methylumbelliferyl 3-O-β-cellobiosyl-β-D-glucosidepre-steady-state initial rates v0 = kobs·ΔP + vss (eqn 5) | 7 | 1.1 | 6.5 | pH 7.2, 30 °C | Open record: 1,3-1,4-β-glucanase, 4-methylumbelliferyl 3-O-β-cellobiosyl-β-D-glucoside |
| 1,3-1,4-β-glucanase | Bacillus licheniformis | 4-methylumbelliferyl 3-O-β-cellotetraosyl-β-D-glucoside | 0.6 | 0.6 | 0.8 | pH 7.2, 30 °C | Open record: 1,3-1,4-β-glucanase, 4-methylumbelliferyl 3-O-β-cellotetraosyl-β-D-glucoside |
| 1,3-1,4-β-glucanase | Bacillus licheniformis | 4-methylumbelliferyl 3-O-β-cellotetraosyl-β-D-glucosidepre-steady-state initial rates v0 = kobs·ΔP + vss (eqn 5) | 3.3 | 0.54 | 6.1 | pH 7.2, 30 °C | Open record: 1,3-1,4-β-glucanase, 4-methylumbelliferyl 3-O-β-cellotetraosyl-β-D-glucoside |
| 1,3-1,4-β-glucanase | Bacillus licheniformis | 4-methylumbelliferyl 3-O-β-cellotriosyl-β-D-glucoside | 0.4 | 0.5 | 0.8 | pH 7.2, 30 °C | Open record: 1,3-1,4-β-glucanase, 4-methylumbelliferyl 3-O-β-cellotriosyl-β-D-glucoside |
| 1,3-1,4-β-glucanase | Bacillus licheniformis | 4-methylumbelliferyl 3-O-β-cellotriosyl-β-D-glucosidepre-steady-state initial rates v0 = kobs·ΔP + vss (eqn 5) | 4 | 0.7 | 5.8 | pH 7.2, 30 °C | Open record: 1,3-1,4-β-glucanase, 4-methylumbelliferyl 3-O-β-cellotriosyl-β-D-glucoside |