EC 3.2.2.22: rRNA N-glycosylase

HydrolasesOpen in Browse

CatLog read 23 records for EC 3.2.2.22 from 2 papers, for 2 organisms. Names in these papers: ricin A-chain (RTA), saporin-L1, saporin-S6.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units.

CatLog also holds 5 records for EC 3.2.2.22 that are not yet re-read: from its earlier version, built from the enzyme databases and partly checked against the papers. All records for EC 3.2.2.22 in Browse.

Papers

Papers with records for EC 3.2.2.22
Paper	Organisms	Records
Ricin A-chain substrate specificity in RNA, DNA, and hybrid stem-loop structures	Ricinus communis (castor bean)	19
Transition state analogues rescue ribosomes from saporin-L1 ribosome inactivating protein	Saponaria officinalis	4

Records

Records for EC 3.2.2.22 read from the paper
Enzyme	Organism	Substrate	k_cat (s⁻¹)	K_m (mM)	k_cat/K_m (mM⁻¹ s⁻¹)	Conditions	Paper	Open record
ricin A-chain (RTA)	Ricinus communis (castor bean)	DNA 12mer stem-loop d(5'-GCGCGAGAGCGC-3')	0.0083	0.0084	0.99	pH 4	PMID 15109249	Open record: ricin A-chain (RTA), DNA 12mer stem-loop d(5'-GCGCGAGAGCGC-3')
ricin A-chain (RTA)	Ricinus communis (castor bean)	DNA 12mer stem-loop d(5'-GCGCGAGAGCGC-3') with a ribonucleoside (A) at position 6 (second loop residue, depurination site)	0.0015	0.0152	0.094	pH 4	PMID 15109249	Open record: ricin A-chain (RTA), DNA 12mer stem-loop d(5'-GCGCGAGAGCGC-3') with a ribonucleoside (A) at position 6 (second loop residue, depurination site)
ricin A-chain (RTA)	Ricinus communis (castor bean)	DNA 12mer stem-loop d(5'-GCGCGAGAGCGC-3') with a ribonucleoside (A) at position 8 (fourth loop residue)	0.0038	0.0094	0.41	pH 4	PMID 15109249	Open record: ricin A-chain (RTA), DNA 12mer stem-loop d(5'-GCGCGAGAGCGC-3') with a ribonucleoside (A) at position 8 (fourth loop residue)
ricin A-chain (RTA)	Ricinus communis (castor bean)	DNA 12mer stem-loop d(5'-GCGCGAGAGCGC-3') with a ribonucleoside (G) at position 5 (first loop residue)	0.015	0.005	3	pH 4	PMID 15109249	Open record: ricin A-chain (RTA), DNA 12mer stem-loop d(5'-GCGCGAGAGCGC-3') with a ribonucleoside (G) at position 5 (first loop residue)
ricin A-chain (RTA)	Ricinus communis (castor bean)	DNA 12mer stem-loop d(5'-GCGCGAGAGCGC-3') with a ribonucleoside (G) at position 7 (third loop residue)	0.0083	0.01	0.8	pH 4	PMID 15109249	Open record: ricin A-chain (RTA), DNA 12mer stem-loop d(5'-GCGCGAGAGCGC-3') with a ribonucleoside (G) at position 7 (third loop residue)
ricin A-chain (RTA)	Ricinus communis (castor bean)	DNA stem with RNA GAGA loop: 5'-d(GCGCr[GAGA]GCGC)-3'	0.0017	0.01	0.17	pH 4	PMID 15109249	Open record: ricin A-chain (RTA), DNA stem with RNA GAGA loop: 5'-d(GCGCr[GAGA]GCGC)-3'
ricin A-chain (RTA)	Ricinus communis (castor bean)	RNA 10mer stem-loop 5'-C1G2C3G4A5G6A7G8C9G10-3'	0.025	0.0013	19 calc.	pH 4	PMID 15109249	Open record: ricin A-chain (RTA), RNA 10mer stem-loop 5'-C1G2C3G4A5G6A7G8C9G10-3'
ricin A-chain (RTA)	Ricinus communis (castor bean)	RNA 12mer stem-loop 5'-GCGC-dG-A-dG-A-GCGC-3' (2'-deoxyguanosine at positions 5 and 7)	—	—	0.0066	pH 4	PMID 15109249	Open record: ricin A-chain (RTA), RNA 12mer stem-loop 5'-GCGC-dG-A-dG-A-GCGC-3' (2'-deoxyguanosine at positions 5 and 7)
ricin A-chain (RTA)	Ricinus communis (castor bean)	RNA 12mer stem-loop 5'-GCGC-dG-AG-dA-GCGC-3' (2'-deoxy at positions 5 and 8)	0.01	0.0018	5.5	pH 4	PMID 15109249	Open record: ricin A-chain (RTA), RNA 12mer stem-loop 5'-GCGC-dG-AG-dA-GCGC-3' (2'-deoxy at positions 5 and 8)
ricin A-chain (RTA)	Ricinus communis (castor bean)	RNA 12mer stem-loop 5'-GCGCGAGAGCGC-3'	1.68	0.0027	620	pH 4	PMID 15109249	Open record: ricin A-chain (RTA), RNA 12mer stem-loop 5'-GCGCGAGAGCGC-3'
ricin A-chain (RTA)	Ricinus communis (castor bean)	RNA 12mer stem-loop 5'-GCGCGAGAGCGC-3' with 2'-deoxyadenosine at position 6 (depurination site)	27.7	0.032	860	pH 4	PMID 15109249	Open record: ricin A-chain (RTA), RNA 12mer stem-loop 5'-GCGCGAGAGCGC-3' with 2'-deoxyadenosine at position 6 (depurination site)
ricin A-chain (RTA)	Ricinus communis (castor bean)	RNA 12mer stem-loop 5'-GCGCGAGAGCGC-3' with 2'-deoxyadenosine at position 8	0.055	0.0057	9.5	pH 4	PMID 15109249	Open record: ricin A-chain (RTA), RNA 12mer stem-loop 5'-GCGCGAGAGCGC-3' with 2'-deoxyadenosine at position 8
ricin A-chain (RTA)	Ricinus communis (castor bean)	RNA 12mer stem-loop 5'-GCGCGAGAGCGC-3' with 2'-deoxyguanosine at position 5	0.0053	7 × 10⁻⁴	7.5	pH 4	PMID 15109249	Open record: ricin A-chain (RTA), RNA 12mer stem-loop 5'-GCGCGAGAGCGC-3' with 2'-deoxyguanosine at position 5
ricin A-chain (RTA)	Ricinus communis (castor bean)	RNA 12mer stem-loop 5'-GCGCGAGAGCGC-3' with 2'-deoxyguanosine at position 7	0.0105	9 × 10⁻⁴	12	pH 4	PMID 15109249	Open record: ricin A-chain (RTA), RNA 12mer stem-loop 5'-GCGCGAGAGCGC-3' with 2'-deoxyguanosine at position 7
ricin A-chain (RTA)	Ricinus communis (castor bean)	RNA 12mer stem-loop 5'-GCGCGAGAGCGC-3' with 2'-O-methyl (printed 2'-O-methoxy) adenosine at position 6 (depurination site)	—	—	0.45	pH 4	PMID 15109249	Open record: ricin A-chain (RTA), RNA 12mer stem-loop 5'-GCGCGAGAGCGC-3' with 2'-O-methyl (printed 2'-O-methoxy) adenosine at position 6 (depurination site)
ricin A-chain (RTA)	Ricinus communis (castor bean)	RNA 12mer stem-loop 5'-GCGCGAGAGCGC-3' with 2'-O-methyl (printed 2'-O-methoxy) adenosine at position 8	0.085	0.0097	8.8	pH 4	PMID 15109249	Open record: ricin A-chain (RTA), RNA 12mer stem-loop 5'-GCGCGAGAGCGC-3' with 2'-O-methyl (printed 2'-O-methoxy) adenosine at position 8
ricin A-chain (RTA)	Ricinus communis (castor bean)	RNA 12mer stem-loop 5'-GCGCGAGAGCGC-3' with 2'-O-methyl (printed 2'-O-methoxy) guanosine at position 5	0.0013	0.0029	0.46	pH 4	PMID 15109249	Open record: ricin A-chain (RTA), RNA 12mer stem-loop 5'-GCGCGAGAGCGC-3' with 2'-O-methyl (printed 2'-O-methoxy) guanosine at position 5
ricin A-chain (RTA)	Ricinus communis (castor bean)	RNA 12mer stem-loop 5'-GCGCGAGAGCGC-3' with 2'-O-methyl (printed 2'-O-methoxy) guanosine at position 7	0.0047	0.0063	0.75	pH 4	PMID 15109249	Open record: ricin A-chain (RTA), RNA 12mer stem-loop 5'-GCGCGAGAGCGC-3' with 2'-O-methyl (printed 2'-O-methoxy) guanosine at position 7
ricin A-chain (RTA)	Ricinus communis (castor bean)	RNA stem with DNA GAGA loop: 5'-GCGCd[GAGA]GCGC-3'	0.048	0.02	2.4	pH 4	PMID 15109249	Open record: ricin A-chain (RTA), RNA stem with DNA GAGA loop: 5'-GCGCd[GAGA]GCGC-3'
saporin-L1	Saponaria officinalis	covalently closed circular G-A-G-A oligonucleotide (oxime linker)	5.02	0.082	61	pH 7.7	PMID 19764816	Open record: saporin-L1, covalently closed circular G-A-G-A oligonucleotide (oxime linker)
saporin-L1	Saponaria officinalis	linear G-A-G-A oligonucleotide	4.88	0.266	18	pH 7.7	PMID 19764816	Open record: saporin-L1, linear G-A-G-A oligonucleotide
saporin-L1	Saponaria officinalis	RNA stem-loop 5'-CGCGAGAGCG-3', a GAGA-tetraloop mimic of the sarcin-ricin loop	7.33	0.095	77	pH 7.7	PMID 19764816	Open record: saporin-L1, RNA stem-loop 5'-CGCGAGAGCG-3', a GAGA-tetraloop mimic of the sarcin-ricin loop
saporin-S6	Saponaria officinalis	RNA stem-loop 5'-CGCGAGAGCG-3', a GAGA-tetraloop mimic of the sarcin-ricin loop	0.0058	0.36	0.016	pH 7.7	PMID 19764816	Open record: saporin-S6, RNA stem-loop 5'-CGCGAGAGCG-3', a GAGA-tetraloop mimic of the sarcin-ricin loop