CatLogenzyme kinetics catalog

Values CatLog read from this paper

Ricin A-chain substrate specificity in RNA, DNA, and hybrid stem-loop structures

PMID 15109249doi:10.1021/bi0498508Open in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 19 records (52 values) from this paper: ricin A-chain (RTA) from Ricinus communis (castor bean).

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 1 kcat/Km value marked “calc.” from kcat and Km; no printed kcat/Km was read for that record.

EC 3.2.2.22

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)Conditions
ricin A-chain (RTA)Ricinus communis (castor bean)DNA 12mer stem-loop d(5'-GCGCGAGAGCGC-3')0.00830.00840.99pH 4Open
ricin A-chain (RTA)Ricinus communis (castor bean)DNA 12mer stem-loop d(5'-GCGCGAGAGCGC-3') with a ribonucleoside (A) at position 6 (second loop residue, depurination site)0.00150.01520.094pH 4Open
ricin A-chain (RTA)Ricinus communis (castor bean)DNA 12mer stem-loop d(5'-GCGCGAGAGCGC-3') with a ribonucleoside (A) at position 8 (fourth loop residue)0.00380.00940.41pH 4Open
ricin A-chain (RTA)Ricinus communis (castor bean)DNA 12mer stem-loop d(5'-GCGCGAGAGCGC-3') with a ribonucleoside (G) at position 5 (first loop residue)0.0150.0053pH 4Open
ricin A-chain (RTA)Ricinus communis (castor bean)DNA 12mer stem-loop d(5'-GCGCGAGAGCGC-3') with a ribonucleoside (G) at position 7 (third loop residue)0.00830.010.8pH 4Open
ricin A-chain (RTA)Ricinus communis (castor bean)DNA stem with RNA GAGA loop: 5'-d(GCGCr[GAGA]GCGC)-3'0.00170.010.17pH 4Open
ricin A-chain (RTA)Ricinus communis (castor bean)RNA 10mer stem-loop 5'-C1G2C3G4A5G6A7G8C9G10-3'0.0250.001319 calc.pH 4Open
ricin A-chain (RTA)Ricinus communis (castor bean)RNA 12mer stem-loop 5'-GCGC-dG-A-dG-A-GCGC-3' (2'-deoxyguanosine at positions 5 and 7)——0.0066pH 4Open
ricin A-chain (RTA)Ricinus communis (castor bean)RNA 12mer stem-loop 5'-GCGC-dG-AG-dA-GCGC-3' (2'-deoxy at positions 5 and 8)0.010.00185.5pH 4Open
ricin A-chain (RTA)Ricinus communis (castor bean)RNA 12mer stem-loop 5'-GCGCGAGAGCGC-3'1.680.0027620pH 4Open
ricin A-chain (RTA)Ricinus communis (castor bean)RNA 12mer stem-loop 5'-GCGCGAGAGCGC-3' with 2'-deoxyadenosine at position 6 (depurination site)27.70.032860pH 4Open
ricin A-chain (RTA)Ricinus communis (castor bean)RNA 12mer stem-loop 5'-GCGCGAGAGCGC-3' with 2'-deoxyadenosine at position 80.0550.00579.5pH 4Open
ricin A-chain (RTA)Ricinus communis (castor bean)RNA 12mer stem-loop 5'-GCGCGAGAGCGC-3' with 2'-deoxyguanosine at position 50.00537 × 10⁻⁴7.5pH 4Open
ricin A-chain (RTA)Ricinus communis (castor bean)RNA 12mer stem-loop 5'-GCGCGAGAGCGC-3' with 2'-deoxyguanosine at position 70.01059 × 10⁻⁴12pH 4Open
ricin A-chain (RTA)Ricinus communis (castor bean)RNA 12mer stem-loop 5'-GCGCGAGAGCGC-3' with 2'-O-methyl (printed 2'-O-methoxy) adenosine at position 6 (depurination site)——0.45pH 4Open
ricin A-chain (RTA)Ricinus communis (castor bean)RNA 12mer stem-loop 5'-GCGCGAGAGCGC-3' with 2'-O-methyl (printed 2'-O-methoxy) adenosine at position 80.0850.00978.8pH 4Open
ricin A-chain (RTA)Ricinus communis (castor bean)RNA 12mer stem-loop 5'-GCGCGAGAGCGC-3' with 2'-O-methyl (printed 2'-O-methoxy) guanosine at position 50.00130.00290.46pH 4Open
ricin A-chain (RTA)Ricinus communis (castor bean)RNA 12mer stem-loop 5'-GCGCGAGAGCGC-3' with 2'-O-methyl (printed 2'-O-methoxy) guanosine at position 70.00470.00630.75pH 4Open
ricin A-chain (RTA)Ricinus communis (castor bean)RNA stem with DNA GAGA loop: 5'-GCGCd[GAGA]GCGC-3'0.0480.022.4pH 4Open