EC 3.2.2.23: DNA-formamidopyrimidine glycosylase
HydrolasesOpen in Browse
CatLog read 40 records for EC 3.2.2.23 from 2 papers, for 1 organism. Names in these papers: formamidopyrimidine-DNA glycosylase (Fpg, MutM).
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units.
CatLog also holds 24 records for EC 3.2.2.23 that are not yet re-read: from its earlier version, built from the enzyme databases and partly checked against the papers. All records for EC 3.2.2.23 in Browse.
Papers
| Paper | Organisms | Records |
|---|---|---|
| Critical Sites of DNA Backbone Integrity for Damaged Base Removal by Formamidopyrimidine-DNA Glycosylase | Escherichia coli | 22 |
| Substrate discrimination by formamidopyrimidine-DNA glycosylase: a mutational analysis | Escherichia coli | 18 |
Records
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Paper | Open record |
|---|---|---|---|---|---|---|---|---|
| formamidopyrimidine-DNA glycosylase (Fpg, MutM) | Escherichia coli | 8-oxoguanine (oxoG):C DNA duplex with a single-strand break (all phosphates kept) disrupting the A(+2)−p(+2) phosphoester link of the complementary (nondamaged) strand | 0.025 | 2.1 × 10⁻⁵ | 1.18 × 10³ | pH 7.5, 30 °C | PMID 31120733 | Open record: formamidopyrimidine-DNA glycosylase (Fpg, MutM), 8-oxoguanine (oxoG):C DNA duplex with a single-strand break (all phosphates kept) disrupting the A(+2)−p(+2) phosphoester link of the complementary (nondamaged) strand |
| formamidopyrimidine-DNA glycosylase (Fpg, MutM) | Escherichia coli | 8-oxoguanine (oxoG):C DNA duplex with a single-strand break (all phosphates kept) disrupting the A(−2)−p(−2) phosphoester link of the complementary (nondamaged) strand | 0.0138 | 2.7 × 10⁻⁵ | 520 | pH 7.5, 30 °C | PMID 31120733 | Open record: formamidopyrimidine-DNA glycosylase (Fpg, MutM), 8-oxoguanine (oxoG):C DNA duplex with a single-strand break (all phosphates kept) disrupting the A(−2)−p(−2) phosphoester link of the complementary (nondamaged) strand |
| formamidopyrimidine-DNA glycosylase (Fpg, MutM) | Escherichia coli | 8-oxoguanine (oxoG):C DNA duplex with a single-strand break (all phosphates kept) disrupting the C(0)−p(0) phosphoester link of the complementary (nondamaged) strand | 0.0108 | 3 × 10⁻⁵ | 370 | pH 7.5, 30 °C | PMID 31120733 | Open record: formamidopyrimidine-DNA glycosylase (Fpg, MutM), 8-oxoguanine (oxoG):C DNA duplex with a single-strand break (all phosphates kept) disrupting the C(0)−p(0) phosphoester link of the complementary (nondamaged) strand |
| formamidopyrimidine-DNA glycosylase (Fpg, MutM) | Escherichia coli | 8-oxoguanine (oxoG):C DNA duplex with a single-strand break (all phosphates kept) disrupting the C+1−p0 phosphoester link of the oxoG-containing (damaged) strand | 0.045 | 1.4 × 10⁻⁵ | 3.2 × 10³ | pH 7.5, 30 °C | PMID 31120733 | Open record: formamidopyrimidine-DNA glycosylase (Fpg, MutM), 8-oxoguanine (oxoG):C DNA duplex with a single-strand break (all phosphates kept) disrupting the C+1−p0 phosphoester link of the oxoG-containing (damaged) strand |
| formamidopyrimidine-DNA glycosylase (Fpg, MutM) | Escherichia coli | 8-oxoguanine (oxoG):C DNA duplex with a single-strand break (all phosphates kept) disrupting the C−1−p−2 phosphoester link of the oxoG-containing (damaged) strand | 0.0037 | 8.2 × 10⁻⁶ | 450 | pH 7.5, 30 °C | PMID 31120733 | Open record: formamidopyrimidine-DNA glycosylase (Fpg, MutM), 8-oxoguanine (oxoG):C DNA duplex with a single-strand break (all phosphates kept) disrupting the C−1−p−2 phosphoester link of the oxoG-containing (damaged) strand |
| formamidopyrimidine-DNA glycosylase (Fpg, MutM) | Escherichia coli | 8-oxoguanine (oxoG):C DNA duplex with a single-strand break (all phosphates kept) disrupting the G(+1)−p(+1) phosphoester link of the complementary (nondamaged) strand | 0.014 | 2.7 × 10⁻⁵ | 520 | pH 7.5, 30 °C | PMID 31120733 | Open record: formamidopyrimidine-DNA glycosylase (Fpg, MutM), 8-oxoguanine (oxoG):C DNA duplex with a single-strand break (all phosphates kept) disrupting the G(+1)−p(+1) phosphoester link of the complementary (nondamaged) strand |
| formamidopyrimidine-DNA glycosylase (Fpg, MutM) | Escherichia coli | 8-oxoguanine (oxoG):C DNA duplex with a single-strand break (all phosphates kept) disrupting the G(−1)−p(−1) phosphoester link of the complementary (nondamaged) strand | 0.028 | 2.8 × 10⁻⁵ | 1.02 × 10³ | pH 7.5, 30 °C | PMID 31120733 | Open record: formamidopyrimidine-DNA glycosylase (Fpg, MutM), 8-oxoguanine (oxoG):C DNA duplex with a single-strand break (all phosphates kept) disrupting the G(−1)−p(−1) phosphoester link of the complementary (nondamaged) strand |
| formamidopyrimidine-DNA glycosylase (Fpg, MutM) | Escherichia coli | 8-oxoguanine (oxoG):C DNA duplex with a single-strand break (all phosphates kept) disrupting the G(−3)−p(−3) phosphoester link of the complementary (nondamaged) strand | 0.018 | 1.7 × 10⁻⁵ | 1.08 × 10³ | pH 7.5, 30 °C | PMID 31120733 | Open record: formamidopyrimidine-DNA glycosylase (Fpg, MutM), 8-oxoguanine (oxoG):C DNA duplex with a single-strand break (all phosphates kept) disrupting the G(−3)−p(−3) phosphoester link of the complementary (nondamaged) strand |
| formamidopyrimidine-DNA glycosylase (Fpg, MutM) | Escherichia coli | 8-oxoguanine (oxoG):C DNA duplex with a single-strand break (all phosphates kept) disrupting the p(+1)−A(+2) phosphoester link of the complementary (nondamaged) strand | 0.0113 | 1.4 × 10⁻⁵ | 820 | pH 7.5, 30 °C | PMID 31120733 | Open record: formamidopyrimidine-DNA glycosylase (Fpg, MutM), 8-oxoguanine (oxoG):C DNA duplex with a single-strand break (all phosphates kept) disrupting the p(+1)−A(+2) phosphoester link of the complementary (nondamaged) strand |
| formamidopyrimidine-DNA glycosylase (Fpg, MutM) | Escherichia coli | 8-oxoguanine (oxoG):C DNA duplex with a single-strand break (all phosphates kept) disrupting the p(+2)−A(+3) phosphoester link of the complementary (nondamaged) strand | 0.022 | 1.8 × 10⁻⁵ | 1.2 × 10³ | pH 7.5, 30 °C | PMID 31120733 | Open record: formamidopyrimidine-DNA glycosylase (Fpg, MutM), 8-oxoguanine (oxoG):C DNA duplex with a single-strand break (all phosphates kept) disrupting the p(+2)−A(+3) phosphoester link of the complementary (nondamaged) strand |
| formamidopyrimidine-DNA glycosylase (Fpg, MutM) | Escherichia coli | 8-oxoguanine (oxoG):C DNA duplex with a single-strand break (all phosphates kept) disrupting the p(0)−G(+1) phosphoester link of the complementary (nondamaged) strand | 0.017 | 3.6 × 10⁻⁵ | 470 | pH 7.5, 30 °C | PMID 31120733 | Open record: formamidopyrimidine-DNA glycosylase (Fpg, MutM), 8-oxoguanine (oxoG):C DNA duplex with a single-strand break (all phosphates kept) disrupting the p(0)−G(+1) phosphoester link of the complementary (nondamaged) strand |
| formamidopyrimidine-DNA glycosylase (Fpg, MutM) | Escherichia coli | 8-oxoguanine (oxoG):C DNA duplex with a single-strand break (all phosphates kept) disrupting the p(−1)−C(0) phosphoester link of the complementary (nondamaged) strand | 0.0133 | 2.8 × 10⁻⁵ | 480 | pH 7.5, 30 °C | PMID 31120733 | Open record: formamidopyrimidine-DNA glycosylase (Fpg, MutM), 8-oxoguanine (oxoG):C DNA duplex with a single-strand break (all phosphates kept) disrupting the p(−1)−C(0) phosphoester link of the complementary (nondamaged) strand |
| formamidopyrimidine-DNA glycosylase (Fpg, MutM) | Escherichia coli | 8-oxoguanine (oxoG):C DNA duplex with a single-strand break (all phosphates kept) disrupting the p(−2)−G(−1) phosphoester link of the complementary (nondamaged) strand | 0.01 | 1.2 × 10⁻⁵ | 830 | pH 7.5, 30 °C | PMID 31120733 | Open record: formamidopyrimidine-DNA glycosylase (Fpg, MutM), 8-oxoguanine (oxoG):C DNA duplex with a single-strand break (all phosphates kept) disrupting the p(−2)−G(−1) phosphoester link of the complementary (nondamaged) strand |
| formamidopyrimidine-DNA glycosylase (Fpg, MutM) | Escherichia coli | 8-oxoguanine (oxoG):C DNA duplex with a single-strand break (all phosphates kept) disrupting the p(−3)−A(−2) phosphoester link of the complementary (nondamaged) strand | 0.015 | 2.3 × 10⁻⁵ | 650 | pH 7.5, 30 °C | PMID 31120733 | Open record: formamidopyrimidine-DNA glycosylase (Fpg, MutM), 8-oxoguanine (oxoG):C DNA duplex with a single-strand break (all phosphates kept) disrupting the p(−3)−A(−2) phosphoester link of the complementary (nondamaged) strand |
| formamidopyrimidine-DNA glycosylase (Fpg, MutM) | Escherichia coli | 8-oxoguanine (oxoG):C DNA duplex with a single-strand break (all phosphates kept) disrupting the p+1−C+1 phosphoester link of the oxoG-containing (damaged) strand | 0.047 | 2.3 × 10⁻⁵ | 2 × 10³ | pH 7.5, 30 °C | PMID 31120733 | Open record: formamidopyrimidine-DNA glycosylase (Fpg, MutM), 8-oxoguanine (oxoG):C DNA duplex with a single-strand break (all phosphates kept) disrupting the p+1−C+1 phosphoester link of the oxoG-containing (damaged) strand |
| formamidopyrimidine-DNA glycosylase (Fpg, MutM) | Escherichia coli | 8-oxoguanine (oxoG):C DNA duplex with a single-strand break (all phosphates kept) disrupting the p+2−T+2 phosphoester link of the oxoG-containing (damaged) strand | 0.023 | 7.5 × 10⁻⁶ | 3.2 × 10³ | pH 7.5, 30 °C | PMID 31120733 | Open record: formamidopyrimidine-DNA glycosylase (Fpg, MutM), 8-oxoguanine (oxoG):C DNA duplex with a single-strand break (all phosphates kept) disrupting the p+2−T+2 phosphoester link of the oxoG-containing (damaged) strand |
| formamidopyrimidine-DNA glycosylase (Fpg, MutM) | Escherichia coli | 8-oxoguanine (oxoG):C DNA duplex with a single-strand break (all phosphates kept) disrupting the p−3−C−3 phosphoester link of the oxoG-containing (damaged) strand | 0.0138 | 1.2 × 10⁻⁵ | 1.15 × 10³ | pH 7.5, 30 °C | PMID 31120733 | Open record: formamidopyrimidine-DNA glycosylase (Fpg, MutM), 8-oxoguanine (oxoG):C DNA duplex with a single-strand break (all phosphates kept) disrupting the p−3−C−3 phosphoester link of the oxoG-containing (damaged) strand |
| formamidopyrimidine-DNA glycosylase (Fpg, MutM) | Escherichia coli | 8-oxoguanine (oxoG):C DNA duplex with a single-strand break (all phosphates kept) disrupting the T+2−p+1 phosphoester link of the oxoG-containing (damaged) strand | 0.02 | 4.8 × 10⁻⁵ | 420 | pH 7.5, 30 °C | PMID 31120733 | Open record: formamidopyrimidine-DNA glycosylase (Fpg, MutM), 8-oxoguanine (oxoG):C DNA duplex with a single-strand break (all phosphates kept) disrupting the T+2−p+1 phosphoester link of the oxoG-containing (damaged) strand |
| formamidopyrimidine-DNA glycosylase (Fpg, MutM) | Escherichia coli | 8-oxoguanine (oxoG):C DNA duplex with a single-strand break (all phosphates kept) disrupting the T+3−p+2 phosphoester link of the oxoG-containing (damaged) strand | 0.022 | 7 × 10⁻⁶ | 3.2 × 10³ | pH 7.5, 30 °C | PMID 31120733 | Open record: formamidopyrimidine-DNA glycosylase (Fpg, MutM), 8-oxoguanine (oxoG):C DNA duplex with a single-strand break (all phosphates kept) disrupting the T+3−p+2 phosphoester link of the oxoG-containing (damaged) strand |
| formamidopyrimidine-DNA glycosylase (Fpg, MutM) | Escherichia coli | 8-oxoguanine (oxoG):C DNA duplex with a single-strand break (all phosphates kept) disrupting the T−2−p−3 phosphoester link of the oxoG-containing (damaged) strand | 0.0147 | 1.1 × 10⁻⁵ | 1.33 × 10³ | pH 7.5, 30 °C | PMID 31120733 | Open record: formamidopyrimidine-DNA glycosylase (Fpg, MutM), 8-oxoguanine (oxoG):C DNA duplex with a single-strand break (all phosphates kept) disrupting the T−2−p−3 phosphoester link of the oxoG-containing (damaged) strand |
| formamidopyrimidine-DNA glycosylase (Fpg, MutM) | Escherichia coli | 8-oxoguanine (oxoG):C DNA duplex with a single-strand break (all phosphates kept; 3′-terminal 2′,3′-dideoxynucleotide at the break) disrupting the ddT−2−p−3 phosphoester link of the oxoG-containing (damaged) strand | 0.0092 | 4 × 10⁻⁶ | 2.3 × 10³ | pH 7.5, 30 °C | PMID 31120733 | Open record: formamidopyrimidine-DNA glycosylase (Fpg, MutM), 8-oxoguanine (oxoG):C DNA duplex with a single-strand break (all phosphates kept; 3′-terminal 2′,3′-dideoxynucleotide at the break) disrupting the ddT−2−p−3 phosphoester link of the oxoG-containing (damaged) strand |
| formamidopyrimidine-DNA glycosylase (Fpg, MutM) | Escherichia coli | 8-oxoguanine (oxoG):C-containing DNA duplex without a break (40-mer) | 0.0055 | 9.7 × 10⁻⁶ | 570 | pH 7.5, 30 °C | PMID 31120733 | Open record: formamidopyrimidine-DNA glycosylase (Fpg, MutM), 8-oxoguanine (oxoG):C-containing DNA duplex without a break (40-mer) |
| formamidopyrimidine-DNA glycosylase (Fpg, MutM) | Escherichia coli | duplex oligodeoxynucleotide containing 5,6-dihydrouracil opposite C | 0.0117 | 6.8 × 10⁻⁷ | 1.72 × 10⁴ | PMID 14607836 | Open record: formamidopyrimidine-DNA glycosylase (Fpg, MutM), duplex oligodeoxynucleotide containing 5,6-dihydrouracil opposite C | |
| formamidopyrimidine-DNA glycosylase (Fpg, MutM) | Escherichia coli | duplex oligodeoxynucleotide containing 8-oxo-7,8-dihydroguanine opposite A | 0.0011 | 2.2 × 10⁻⁵ | 50 | PMID 14607836 | Open record: formamidopyrimidine-DNA glycosylase (Fpg, MutM), duplex oligodeoxynucleotide containing 8-oxo-7,8-dihydroguanine opposite A | |
| formamidopyrimidine-DNA glycosylase (Fpg, MutM) | Escherichia coli | duplex oligodeoxynucleotide containing 8-oxo-7,8-dihydroguanine opposite C | 0.0053 | 1.05 × 10⁻⁶ | 5.2 × 10³ | PMID 14607836 | Open record: formamidopyrimidine-DNA glycosylase (Fpg, MutM), duplex oligodeoxynucleotide containing 8-oxo-7,8-dihydroguanine opposite C | |
| formamidopyrimidine-DNA glycosylase (Fpg, MutM) | Escherichia coli | duplex oligodeoxynucleotide containing 8-oxo-7,8-dihydroguanine opposite G | 0.0035 | 1 × 10⁻⁷ | 3.43 × 10⁴ | PMID 14607836 | Open record: formamidopyrimidine-DNA glycosylase (Fpg, MutM), duplex oligodeoxynucleotide containing 8-oxo-7,8-dihydroguanine opposite G | |
| formamidopyrimidine-DNA glycosylase (Fpg, MutM) | Escherichia coli | duplex oligodeoxynucleotide containing 8-oxo-7,8-dihydroguanine opposite T | 0.0047 | 1.9 × 10⁻⁷ | 2.42 × 10⁴ | PMID 14607836 | Open record: formamidopyrimidine-DNA glycosylase (Fpg, MutM), duplex oligodeoxynucleotide containing 8-oxo-7,8-dihydroguanine opposite T | |
| formamidopyrimidine-DNA glycosylase (Fpg, MutM)variant H89A | Escherichia coli | duplex oligodeoxynucleotide containing 5,6-dihydrouracil opposite C | 0.0163 | 7 × 10⁻⁷ | 2.33 × 10⁴ | PMID 14607836 | Open record: formamidopyrimidine-DNA glycosylase (Fpg, MutM) variant H89A, duplex oligodeoxynucleotide containing 5,6-dihydrouracil opposite C | |
| formamidopyrimidine-DNA glycosylase (Fpg, MutM)variant H89A | Escherichia coli | duplex oligodeoxynucleotide containing 8-oxo-7,8-dihydroguanine opposite C | 0.0075 | 4.14 × 10⁻⁶ | 1.8 × 10³ | PMID 14607836 | Open record: formamidopyrimidine-DNA glycosylase (Fpg, MutM) variant H89A, duplex oligodeoxynucleotide containing 8-oxo-7,8-dihydroguanine opposite C | |
| formamidopyrimidine-DNA glycosylase (Fpg, MutM)variant H89A/R109A | Escherichia coli | duplex oligodeoxynucleotide containing 5,6-dihydrouracil opposite C | 0.004 | 9.57 × 10⁻⁶ | 420 | PMID 14607836 | Open record: formamidopyrimidine-DNA glycosylase (Fpg, MutM) variant H89A/R109A, duplex oligodeoxynucleotide containing 5,6-dihydrouracil opposite C | |
| formamidopyrimidine-DNA glycosylase (Fpg, MutM)variant H89A/R109A | Escherichia coli | duplex oligodeoxynucleotide containing 8-oxo-7,8-dihydroguanine opposite C | 0.0035 | 4.4 × 10⁻⁴ | 8.3 | PMID 14607836 | Open record: formamidopyrimidine-DNA glycosylase (Fpg, MutM) variant H89A/R109A, duplex oligodeoxynucleotide containing 8-oxo-7,8-dihydroguanine opposite C | |
| formamidopyrimidine-DNA glycosylase (Fpg, MutM)variant K217T | Escherichia coli | duplex oligodeoxynucleotide containing 5,6-dihydrouracil opposite C | 0.0093 | 6.1 × 10⁻⁷ | 1.55 × 10⁴ | PMID 14607836 | Open record: formamidopyrimidine-DNA glycosylase (Fpg, MutM) variant K217T, duplex oligodeoxynucleotide containing 5,6-dihydrouracil opposite C | |
| formamidopyrimidine-DNA glycosylase (Fpg, MutM)variant K217T | Escherichia coli | duplex oligodeoxynucleotide containing 8-oxo-7,8-dihydroguanine opposite C | 0.004 | 4.06 × 10⁻⁶ | 970 | PMID 14607836 | Open record: formamidopyrimidine-DNA glycosylase (Fpg, MutM) variant K217T, duplex oligodeoxynucleotide containing 8-oxo-7,8-dihydroguanine opposite C | |
| formamidopyrimidine-DNA glycosylase (Fpg, MutM)variant R108A | Escherichia coli | duplex oligodeoxynucleotide containing 5,6-dihydrouracil opposite C | 0.0053 | 7 × 10⁻⁷ | 7.7 × 10³ | PMID 14607836 | Open record: formamidopyrimidine-DNA glycosylase (Fpg, MutM) variant R108A, duplex oligodeoxynucleotide containing 5,6-dihydrouracil opposite C | |
| formamidopyrimidine-DNA glycosylase (Fpg, MutM)variant R108A | Escherichia coli | duplex oligodeoxynucleotide containing 8-oxo-7,8-dihydroguanine opposite A | 7.5 × 10⁻⁵ | 2.32 × 10⁻⁵ | 3.3 | PMID 14607836 | Open record: formamidopyrimidine-DNA glycosylase (Fpg, MutM) variant R108A, duplex oligodeoxynucleotide containing 8-oxo-7,8-dihydroguanine opposite A | |
| formamidopyrimidine-DNA glycosylase (Fpg, MutM)variant R108A | Escherichia coli | duplex oligodeoxynucleotide containing 8-oxo-7,8-dihydroguanine opposite C | 4.3 × 10⁻⁴ | 1.49 × 10⁻⁶ | 300 | PMID 14607836 | Open record: formamidopyrimidine-DNA glycosylase (Fpg, MutM) variant R108A, duplex oligodeoxynucleotide containing 8-oxo-7,8-dihydroguanine opposite C | |
| formamidopyrimidine-DNA glycosylase (Fpg, MutM)variant R108A | Escherichia coli | duplex oligodeoxynucleotide containing 8-oxo-7,8-dihydroguanine opposite G | 3.8 × 10⁻⁴ | 5.13 × 10⁻⁶ | 83 | PMID 14607836 | Open record: formamidopyrimidine-DNA glycosylase (Fpg, MutM) variant R108A, duplex oligodeoxynucleotide containing 8-oxo-7,8-dihydroguanine opposite G | |
| formamidopyrimidine-DNA glycosylase (Fpg, MutM)variant R108A | Escherichia coli | duplex oligodeoxynucleotide containing 8-oxo-7,8-dihydroguanine opposite T | 3 × 10⁻⁴ | 4.18 × 10⁻⁶ | 67 | PMID 14607836 | Open record: formamidopyrimidine-DNA glycosylase (Fpg, MutM) variant R108A, duplex oligodeoxynucleotide containing 8-oxo-7,8-dihydroguanine opposite T | |
| formamidopyrimidine-DNA glycosylase (Fpg, MutM)variant R109A | Escherichia coli | duplex oligodeoxynucleotide containing 5,6-dihydrouracil opposite C | 0.0053 | 4.65 × 10⁻⁶ | 1.13 × 10³ | PMID 14607836 | Open record: formamidopyrimidine-DNA glycosylase (Fpg, MutM) variant R109A, duplex oligodeoxynucleotide containing 5,6-dihydrouracil opposite C | |
| formamidopyrimidine-DNA glycosylase (Fpg, MutM)variant R109A | Escherichia coli | duplex oligodeoxynucleotide containing 8-oxo-7,8-dihydroguanine opposite C | 0.0058 | 2.27 × 10⁻⁴ | 33 | PMID 14607836 | Open record: formamidopyrimidine-DNA glycosylase (Fpg, MutM) variant R109A, duplex oligodeoxynucleotide containing 8-oxo-7,8-dihydroguanine opposite C |