CatLogenzyme kinetics catalog

EC 3.4.21.42: complement subcomponent C1s

HydrolasesOpen in Browse

CatLog read 73 records for EC 3.4.21.42 from 5 papers, for 1 organism. Names in these papers: C1s (complement C1s protease), complement C1s, C1s, complement subcomponent C1s (activated C1s).

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units.

CatLog also holds 2 records for EC 3.4.21.42 that are not yet re-read: from its earlier version, built from the enzyme databases and partly checked against the papers. All records for EC 3.4.21.42 in Browse.

Papers

PaperOrganismsRecords
Importance of the prime subsites of the C1s protease of the classical complement pathway for recognition of substratesHomo sapiens44
The role of the lys628 (192) residue of the complement protease, c1s, in interacting with Peptide and protein substratesHomo sapiens9
Functional characterization of complement proteases C1s/mannan-binding lectin-associated serine protease-2 (MASP-2) chimeras reveals the higher C4 recognition efficacy of the MASP-2 complement…Homo sapiens8
Substrate specificities of recombinant mannan-binding lectin-associated serine proteases-1 and -2Homo sapiens6
Elucidation of the substrate specificity of the MASP-2 protease of the lectin complement pathway and identification of the enzyme as a major physiological target of the serpin, C1-inhibitorHomo sapiens6

Records

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)ConditionsPaper
C1sHomo sapiens2-aminobenzoate-GLQRALEI-Lys(Dinitrophenol)-NH21.14——pH 7.4, 37 °CPMID 25278939Open
C1sHomo sapiensZ-AGLQR-AMC35.33——pH 7.4, 37 °CPMID 25278939Open
C1sHomo sapiensZ-LGR-AMC2.72——pH 7.4, 37 °CPMID 25278939Open
C1svariant K628AHomo sapiens2-aminobenzoate-GLQRALEI-Lys(Dinitrophenol)-NH21.3——pH 7.4, 37 °CPMID 25278939Open
C1svariant K628AHomo sapiensZ-AGLQR-AMC1.3——pH 7.4, 37 °CPMID 25278939Open
C1svariant K628AHomo sapiensZ-LGR-AMC1.77——pH 7.4, 37 °CPMID 25278939Open
C1svariant K628QHomo sapiens2-aminobenzoate-GLQRALEI-Lys(Dinitrophenol)-NH21.17——pH 7.4, 37 °CPMID 25278939Open
C1svariant K628QHomo sapiensZ-AGLQR-AMC116.5——pH 7.4, 37 °CPMID 25278939Open
C1svariant K628QHomo sapiensZ-LGR-AMC6——pH 7.4, 37 °CPMID 25278939Open
C1s (complement C1s protease)Homo sapiens2Abz-Gly-Leu-Gln-Arg-Ala-Ala-Glu-Ile-Lys(Dnp)-NH21.69 × 10⁻⁴——pH 7.4, 37 °CPMID 14674770Open
C1s (complement C1s protease)Homo sapiens2Abz-Gly-Leu-Gln-Arg-Ala-Gln-Glu-Ile-Lys(Dnp)-NH21.83 × 10⁻⁴——pH 7.4, 37 °CPMID 14674770Open
C1s (complement C1s protease)Homo sapiens2Abz-Gly-Leu-Gln-Arg-Ala-Glu-Glu-Ile-Lys(Dnp)-NH24.21 × 10⁻⁵——pH 7.4, 37 °CPMID 14674770Open
C1s (complement C1s protease)Homo sapiens2Abz-Gly-Leu-Gln-Arg-Ala-His-Glu-Ile-Lys(Dnp)-NH23.23 × 10⁻⁴——pH 7.4, 37 °CPMID 14674770Open
C1s (complement C1s protease)Homo sapiens2Abz-Gly-Leu-Gln-Arg-Ala-Leu-Ala-Ile-Lys(Dnp)-NH20.00274——pH 7.4, 37 °CPMID 14674770Open
C1s (complement C1s protease)Homo sapiens2Abz-Gly-Leu-Gln-Arg-Ala-Leu-Gln-Ile-Lys(Dnp)-NH20.00232——pH 7.4, 37 °CPMID 14674770Open
C1s (complement C1s protease)Homo sapiens2Abz-Gly-Leu-Gln-Arg-Ala-Leu-Glu-Ala-Lys(Dnp)-NH23.92 × 10⁻⁴——pH 7.4, 37 °CPMID 14674770Open
C1s (complement C1s protease)Homo sapiens2Abz-Gly-Leu-Gln-Arg-Ala-Leu-Glu-Gln-Lys(Dnp)-NH29.78 × 10⁻⁵——pH 7.4, 37 °CPMID 14674770Open
C1s (complement C1s protease)Homo sapiens2Abz-Gly-Leu-Gln-Arg-Ala-Leu-Glu-Glu-Lys(Dnp)-NH29.78 × 10⁻⁵——pH 7.4, 37 °CPMID 14674770Open
C1s (complement C1s protease)Homo sapiens2Abz-Gly-Leu-Gln-Arg-Ala-Leu-Glu-His-Lys(Dnp)-NH22.44 × 10⁻⁴——pH 7.4, 37 °CPMID 14674770Open
C1s (complement C1s protease)Homo sapiens2Abz-Gly-Leu-Gln-Arg-Ala-Leu-Glu-Ile-Lys(Dnp)-NH2commercial (Calbiochem); activated two-chain human C1s2.1 × 10⁻⁴——pH 7.4, 37 °CPMID 14674770Open
C1s (complement C1s protease)Homo sapiens2Abz-Gly-Leu-Gln-Arg-Ala-Leu-Glu-Ile-Lys(Dnp)-NH2+0.15 mM CaCl2; commercial (Calbiochem)8.31 × 10⁻⁴——pH 7.4, 37 °CPMID 14674770Open
C1s (complement C1s protease)Homo sapiens2Abz-Gly-Leu-Gln-Arg-Ala-Leu-Glu-Ile-Lys(Dnp)-NH2+0.15 mM CaCl2; spontaneously activated 30 min at 37 °C0.00178——pH 7.4, 37 °CPMID 14674770Open
C1s (complement C1s protease)Homo sapiens2Abz-Gly-Leu-Gln-Arg-Ala-Leu-Glu-Leu-Lys(Dnp)-NH29.78 × 10⁻⁵——pH 7.4, 37 °CPMID 14674770Open
C1s (complement C1s protease)Homo sapiens2Abz-Gly-Leu-Gln-Arg-Ala-Leu-Glu-Lys(Dnp)-NH23.19 × 10⁻⁴——pH 7.4, 37 °CPMID 14674770Open
C1s (complement C1s protease)Homo sapiens2Abz-Gly-Leu-Gln-Arg-Ala-Leu-Glu-Lys-Lys(Dnp)-NH28.67 × 10⁻⁵——pH 7.4, 37 °CPMID 14674770Open
C1s (complement C1s protease)Homo sapiens2Abz-Gly-Leu-Gln-Arg-Ala-Leu-Glu-Met-Lys(Dnp)-NH21.71 × 10⁻⁴——pH 7.4, 37 °CPMID 14674770Open
C1s (complement C1s protease)Homo sapiens2Abz-Gly-Leu-Gln-Arg-Ala-Leu-Glu-Phe-Lys(Dnp)-NH22.2 × 10⁻⁴——pH 7.4, 37 °CPMID 14674770Open
C1s (complement C1s protease)Homo sapiens2Abz-Gly-Leu-Gln-Arg-Ala-Leu-Glu-Pro-Lys(Dnp)-NH28.07 × 10⁻⁴——pH 7.4, 37 °CPMID 14674770Open
C1s (complement C1s protease)Homo sapiens2Abz-Gly-Leu-Gln-Arg-Ala-Leu-Glu-Thr-Lys(Dnp)-NH24.4 × 10⁻⁴——pH 7.4, 37 °CPMID 14674770Open
C1s (complement C1s protease)Homo sapiens2Abz-Gly-Leu-Gln-Arg-Ala-Leu-His-Ile-Lys(Dnp)-NH20.00821——pH 7.4, 37 °CPMID 14674770Open
C1s (complement C1s protease)Homo sapiens2Abz-Gly-Leu-Gln-Arg-Ala-Leu-Leu-Ile-Lys(Dnp)-NH20.00296——pH 7.4, 37 °CPMID 14674770Open
C1s (complement C1s protease)Homo sapiens2Abz-Gly-Leu-Gln-Arg-Ala-Leu-Lys(Dnp)-NH26.83 × 10⁻⁴——pH 7.4, 37 °CPMID 14674770Open
C1s (complement C1s protease)Homo sapiens2Abz-Gly-Leu-Gln-Arg-Ala-Leu-Lys-Ile-Lys(Dnp)-NH21.36 × 10⁻⁴——pH 7.4, 37 °CPMID 14674770Open
C1s (complement C1s protease)Homo sapiens2Abz-Gly-Leu-Gln-Arg-Ala-Leu-Met-Ile-Lys(Dnp)-NH20.00227——pH 7.4, 37 °CPMID 14674770Open
C1s (complement C1s protease)Homo sapiens2Abz-Gly-Leu-Gln-Arg-Ala-Leu-Phe-Ile-Lys(Dnp)-NH20.00288——pH 7.4, 37 °CPMID 14674770Open
C1s (complement C1s protease)Homo sapiens2Abz-Gly-Leu-Gln-Arg-Ala-Leu-Pro-Ile-Lys(Dnp)-NH20.00244——pH 7.4, 37 °CPMID 14674770Open
C1s (complement C1s protease)Homo sapiens2Abz-Gly-Leu-Gln-Arg-Ala-Leu-Thr-Ile-Lys(Dnp)-NH20.00279——pH 7.4, 37 °CPMID 14674770Open
C1s (complement C1s protease)Homo sapiens2Abz-Gly-Leu-Gln-Arg-Ala-Lys(Dnp)-NH23.94 × 10⁻⁵——pH 7.4, 37 °CPMID 14674770Open
C1s (complement C1s protease)Homo sapiens2Abz-Gly-Leu-Gln-Arg-Ala-Met-Glu-Ile-Lys(Dnp)-NH21.19 × 10⁻⁴——pH 7.4, 37 °CPMID 14674770Open
C1s (complement C1s protease)Homo sapiens2Abz-Gly-Leu-Gln-Arg-Ala-Phe-Glu-Ile-Lys(Dnp)-NH23.3 × 10⁻⁴——pH 7.4, 37 °CPMID 14674770Open
C1s (complement C1s protease)Homo sapiens2Abz-Gly-Leu-Gln-Arg-Ala-Pro-Glu-Ile-Lys(Dnp)-NH21.89 × 10⁻⁵——pH 7.4, 37 °CPMID 14674770Open
C1s (complement C1s protease)Homo sapiens2Abz-Gly-Leu-Gln-Arg-Ala-Thr-Glu-Ile-Lys(Dnp)-NH21.47 × 10⁻⁴——pH 7.4, 37 °CPMID 14674770Open
C1s (complement C1s protease)Homo sapiens2Abz-Gly-Leu-Gln-Arg-Gln-Leu-Glu-Ile-Lys(Dnp)-NH27.02 × 10⁻⁵——pH 7.4, 37 °CPMID 14674770Open
C1s (complement C1s protease)Homo sapiens2Abz-Gly-Leu-Gln-Arg-Glu-Leu-Glu-Ile-Lys(Dnp)-NH22.05 × 10⁻⁵——pH 7.4, 37 °CPMID 14674770Open
C1s (complement C1s protease)Homo sapiens2Abz-Gly-Leu-Gln-Arg-His-Leu-Glu-Ile-Lys(Dnp)-NH24.15 × 10⁻⁴——pH 7.4, 37 °CPMID 14674770Open
C1s (complement C1s protease)Homo sapiens2Abz-Gly-Leu-Gln-Arg-Leu-Leu-Glu-Ile-Lys(Dnp)-NH27.02 × 10⁻⁵——pH 7.4, 37 °CPMID 14674770Open
C1s (complement C1s protease)Homo sapiens2Abz-Gly-Leu-Gln-Arg-Lys-Leu-Glu-Ile-Lys(Dnp)-NH27.62 × 10⁻⁵——pH 7.4, 37 °CPMID 14674770Open
C1s (complement C1s protease)Homo sapiens2Abz-Gly-Leu-Gln-Arg-Met-Leu-Glu-Ile-Lys(Dnp)-NH22.39 × 10⁻⁴——pH 7.4, 37 °CPMID 14674770Open
C1s (complement C1s protease)Homo sapiens2Abz-Gly-Leu-Gln-Arg-Phe-Leu-Glu-Ile-Lys(Dnp)-NH21.55 × 10⁻⁴——pH 7.4, 37 °CPMID 14674770Open
C1s (complement C1s protease)Homo sapiens2Abz-Gly-Leu-Gln-Arg-Thr-Leu-Glu-Ile-Lys(Dnp)-NH20.00155——pH 7.4, 37 °CPMID 14674770Open
C1s (complement C1s protease)Homo sapiens2Abz-Ser-Leu-Gly-Arg-Lys-Ile-Gln-Ile-Lys(Dnp)-NH20.00139——pH 7.4, 37 °CPMID 14674770Open
C1s (complement C1s protease)Homo sapiensZ-Gly-Leu-Gln-Arg-AMC0.380.35811.06pH 7.4, 37 °CPMID 14674770Open
C1s (complement C1s protease)Homo sapiensZ-Ser-Leu-Gly-Arg-NHMec12.890.136994.2pH 7.4, 37 °CPMID 14674770Open
complement C1sHomo sapiens2Abz-GLQRALEI-Lys(Dnp)-NH20.0145——37 °CPMID 17709141Open
complement C1sHomo sapiens2Abz-SLGRKIQI-Lys(Dnp)-NH20.069——37 °CPMID 17709141Open
complement C1sHomo sapiens2Abz-SVARTLLV-Lys(Dnp)-NH20.0388——37 °CPMID 17709141Open
complement C1sHomo sapiensC1-Inh P4-P1 peptide substrate0.3130.34080.937 °CPMID 17709141Open
complement C1sHomo sapiensC2 P4-P1 peptide substrate12.890.13699037 °CPMID 17709141Open
complement C1sHomo sapiensC4 P4-P1 peptide substrate0.380.3581137 °CPMID 17709141Open
complement C1sHomo sapienscomplement component C25.10.0061840PMID 16227207Open
complement C1sHomo sapienscomplement component C45.40.0066820PMID 16227207Open
complement C1schimera C1s(MASP-2 CCP1/2)Homo sapienscomplement component C25.10.0092550PMID 16227207Open
complement C1schimera C1s(MASP-2 CCP1/2)Homo sapienscomplement component C43.21.47 × 10⁻⁴2.18 × 10⁴PMID 16227207Open
complement C1schimera C1s(MASP-2 SP)Homo sapienscomplement component C25.20.0073710PMID 16227207Open
complement C1schimera C1s(MASP-2 SP)Homo sapienscomplement component C42.90.00281.04 × 10³PMID 16227207Open
complement C1svariant D275S/P276I/K405VHomo sapienscomplement component C25.20.0092560PMID 16227207Open
complement C1svariant D275S/P276I/K405VHomo sapienscomplement component C45.30.0051.06 × 10³PMID 16227207Open
complement subcomponent C1s (activated C1s)Homo sapienshuman complement component C2 (purified from human plasma)5.30.0123430pH 7.4, 37 °CPMID 11527969Open
complement subcomponent C1s (activated C1s)Homo sapienshuman complement component C4 (purified from human plasma)4.70.001922.45 × 10³pH 7.4, 37 °CPMID 11527969Open
complement subcomponent C1s (activated C1s)Homo sapiensN-acetylglycyl-L-lysine methyl ester1672.566.830 °CPMID 11527969Open
complement subcomponent C1s (activated C1s)Homo sapiensN-benzyloxycarbonylglycyl-L-arginine thiobenzyl ester24.30.1912830 °CPMID 11527969Open
complement subcomponent C1s (activated C1s)Homo sapiensNalpha-benzoyl-L-arginine ethyl ester9.542.3730 °CPMID 11527969Open
complement subcomponent C1s (activated C1s)Homo sapiensp-tosyl-L-arginine methyl ester11.91.29.930 °CPMID 11527969Open