EC 3.4.21.66: Thermitase

HydrolasesOpen in Browse

CatLog read 14 records for EC 3.4.21.66 from 1 paper, for 1 organism. Names in these papers: Proteolysin PrlA.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units.

Papers

Papers with records for EC 3.4.21.66
Paper	Organisms	Records
Proteolysin, a novel highly thermostable and cosolvent-compatible protease from the thermophilic bacterium Coprothermobacter proteolyticus	Coprothermobacter proteolyticus	14

Records

Records for EC 3.4.21.66 read from the paper
Enzyme	Organism	Substrate	k_cat (s⁻¹)	K_m (mM)	k_cat/K_m (mM⁻¹ s⁻¹)	Conditions	Paper	Open record
Proteolysin PrlA	Coprothermobacter proteolyticus	N-succinyl-Ala-Ala-Pro-Phe p-nitroanilide	25	—	—	pH 7.5, 40 °C	PMID 23851086	Open record: Proteolysin PrlA, N-succinyl-Ala-Ala-Pro-Phe p-nitroanilide
Proteolysin PrlA	Coprothermobacter proteolyticus	N-succinyl-Ala-Ala-Pro-Phe p-nitroanilide	—	0.41	—	pH 7.5, 40 °C	PMID 23851086	Open record: Proteolysin PrlA, N-succinyl-Ala-Ala-Pro-Phe p-nitroanilide
Proteolysin PrlA	Coprothermobacter proteolyticus	N-succinyl-Ala-Ala-Pro-Phe p-nitroanilide	—	—	62	pH 7.5, 40 °C	PMID 23851086	Open record: Proteolysin PrlA, N-succinyl-Ala-Ala-Pro-Phe p-nitroanilide
Proteolysin PrlA	Coprothermobacter proteolyticus	N-succinyl-Ala-Ala-Pro-Phe p-nitroanilide10 mM DTT in assay	37	—	—	pH 7.5, 40 °C	PMID 23851086	Open record: Proteolysin PrlA, N-succinyl-Ala-Ala-Pro-Phe p-nitroanilide
Proteolysin PrlA	Coprothermobacter proteolyticus	N-succinyl-Ala-Ala-Pro-Phe p-nitroanilide10 mM DTT in assay	—	0.57	—	pH 7.5, 40 °C	PMID 23851086	Open record: Proteolysin PrlA, N-succinyl-Ala-Ala-Pro-Phe p-nitroanilide
Proteolysin PrlA	Coprothermobacter proteolyticus	N-succinyl-Ala-Ala-Pro-Phe p-nitroanilide10 mM DTT in assay	—	—	65	pH 7.5, 40 °C	PMID 23851086	Open record: Proteolysin PrlA, N-succinyl-Ala-Ala-Pro-Phe p-nitroanilide
Proteolysin PrlA	Coprothermobacter proteolyticus	N-succinyl-Ala-Ala-Pro-Phe p-nitroanilide	110	1.58	69	pH 7.5, 70 °C	PMID 23851086	Open record: Proteolysin PrlA, N-succinyl-Ala-Ala-Pro-Phe p-nitroanilide
Proteolysin PrlAvariant C182A/C201A	Coprothermobacter proteolyticus	N-succinyl-Ala-Ala-Pro-Phe p-nitroanilide	111	—	—	pH 7.5, 40 °C	PMID 23851086	Open record: Proteolysin PrlA variant C182A/C201A, N-succinyl-Ala-Ala-Pro-Phe p-nitroanilide
Proteolysin PrlAvariant C182A/C201A	Coprothermobacter proteolyticus	N-succinyl-Ala-Ala-Pro-Phe p-nitroanilide	—	0.33	—	pH 7.5, 40 °C	PMID 23851086	Open record: Proteolysin PrlA variant C182A/C201A, N-succinyl-Ala-Ala-Pro-Phe p-nitroanilide
Proteolysin PrlAvariant C182A/C201A	Coprothermobacter proteolyticus	N-succinyl-Ala-Ala-Pro-Phe p-nitroanilide	—	—	335	pH 7.5, 40 °C	PMID 23851086	Open record: Proteolysin PrlA variant C182A/C201A, N-succinyl-Ala-Ala-Pro-Phe p-nitroanilide
Proteolysin PrlAvariant C182A/C201A	Coprothermobacter proteolyticus	N-succinyl-Ala-Ala-Pro-Phe p-nitroanilide10 mM DTT in assay	124	—	—	pH 7.5, 40 °C	PMID 23851086	Open record: Proteolysin PrlA variant C182A/C201A, N-succinyl-Ala-Ala-Pro-Phe p-nitroanilide
Proteolysin PrlAvariant C182A/C201A	Coprothermobacter proteolyticus	N-succinyl-Ala-Ala-Pro-Phe p-nitroanilide10 mM DTT in assay	—	0.66	—	pH 7.5, 40 °C	PMID 23851086	Open record: Proteolysin PrlA variant C182A/C201A, N-succinyl-Ala-Ala-Pro-Phe p-nitroanilide
Proteolysin PrlAvariant C182A/C201A	Coprothermobacter proteolyticus	N-succinyl-Ala-Ala-Pro-Phe p-nitroanilide10 mM DTT in assay	—	—	189	pH 7.5, 40 °C	PMID 23851086	Open record: Proteolysin PrlA variant C182A/C201A, N-succinyl-Ala-Ala-Pro-Phe p-nitroanilide
Proteolysin PrlAvariant C182A/C201A	Coprothermobacter proteolyticus	N-succinyl-Ala-Ala-Pro-Phe p-nitroanilide	248	1.96	126	pH 7.5, 70 °C	PMID 23851086	Open record: Proteolysin PrlA variant C182A/C201A, N-succinyl-Ala-Ala-Pro-Phe p-nitroanilide

CatLog snapshot Oct 24, 2026 (data ID 660092338b). CatLog's compilation is free to reuse under CC BY 4.0; records from a database keep its license. How to cite.