Values CatLog read from this paper
Proteolysin, a novel highly thermostable and cosolvent-compatible protease from the thermophilic bacterium Coprothermobacter proteolyticus
PMID 23851086doi:10.1128/aem.01479-13Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 21 records (27 values) from this paper: Proteolysin PrlA, Subtilisin A from Coprothermobacter proteolyticus.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| Proteolysin PrlA | Coprothermobacter proteolyticus | N-succinyl-Ala-Ala-Pro-Phe p-nitroanilide | 25 | — | — | pH 7.5, 40 °C | Open record: Proteolysin PrlA, N-succinyl-Ala-Ala-Pro-Phe p-nitroanilide |
| Proteolysin PrlA | Coprothermobacter proteolyticus | N-succinyl-Ala-Ala-Pro-Phe p-nitroanilide | — | 0.41 | — | pH 7.5, 40 °C | Open record: Proteolysin PrlA, N-succinyl-Ala-Ala-Pro-Phe p-nitroanilide |
| Proteolysin PrlA | Coprothermobacter proteolyticus | N-succinyl-Ala-Ala-Pro-Phe p-nitroanilide | — | — | 62 | pH 7.5, 40 °C | Open record: Proteolysin PrlA, N-succinyl-Ala-Ala-Pro-Phe p-nitroanilide |
| Proteolysin PrlA | Coprothermobacter proteolyticus | N-succinyl-Ala-Ala-Pro-Phe p-nitroanilide10 mM DTT in assay | 37 | — | — | pH 7.5, 40 °C | Open record: Proteolysin PrlA, N-succinyl-Ala-Ala-Pro-Phe p-nitroanilide |
| Proteolysin PrlA | Coprothermobacter proteolyticus | N-succinyl-Ala-Ala-Pro-Phe p-nitroanilide10 mM DTT in assay | — | 0.57 | — | pH 7.5, 40 °C | Open record: Proteolysin PrlA, N-succinyl-Ala-Ala-Pro-Phe p-nitroanilide |
| Proteolysin PrlA | Coprothermobacter proteolyticus | N-succinyl-Ala-Ala-Pro-Phe p-nitroanilide10 mM DTT in assay | — | — | 65 | pH 7.5, 40 °C | Open record: Proteolysin PrlA, N-succinyl-Ala-Ala-Pro-Phe p-nitroanilide |
| Proteolysin PrlA | Coprothermobacter proteolyticus | N-succinyl-Ala-Ala-Pro-Phe p-nitroanilide | 110 | 1.58 | 69 | pH 7.5, 70 °C | Open record: Proteolysin PrlA, N-succinyl-Ala-Ala-Pro-Phe p-nitroanilide |
| Proteolysin PrlAvariant C182A/C201A | Coprothermobacter proteolyticus | N-succinyl-Ala-Ala-Pro-Phe p-nitroanilide | 111 | — | — | pH 7.5, 40 °C | Open record: Proteolysin PrlA variant C182A/C201A, N-succinyl-Ala-Ala-Pro-Phe p-nitroanilide |
| Proteolysin PrlAvariant C182A/C201A | Coprothermobacter proteolyticus | N-succinyl-Ala-Ala-Pro-Phe p-nitroanilide | — | 0.33 | — | pH 7.5, 40 °C | Open record: Proteolysin PrlA variant C182A/C201A, N-succinyl-Ala-Ala-Pro-Phe p-nitroanilide |
| Proteolysin PrlAvariant C182A/C201A | Coprothermobacter proteolyticus | N-succinyl-Ala-Ala-Pro-Phe p-nitroanilide | — | — | 335 | pH 7.5, 40 °C | Open record: Proteolysin PrlA variant C182A/C201A, N-succinyl-Ala-Ala-Pro-Phe p-nitroanilide |
| Proteolysin PrlAvariant C182A/C201A | Coprothermobacter proteolyticus | N-succinyl-Ala-Ala-Pro-Phe p-nitroanilide10 mM DTT in assay | 124 | — | — | pH 7.5, 40 °C | Open record: Proteolysin PrlA variant C182A/C201A, N-succinyl-Ala-Ala-Pro-Phe p-nitroanilide |
| Proteolysin PrlAvariant C182A/C201A | Coprothermobacter proteolyticus | N-succinyl-Ala-Ala-Pro-Phe p-nitroanilide10 mM DTT in assay | — | 0.66 | — | pH 7.5, 40 °C | Open record: Proteolysin PrlA variant C182A/C201A, N-succinyl-Ala-Ala-Pro-Phe p-nitroanilide |
| Proteolysin PrlAvariant C182A/C201A | Coprothermobacter proteolyticus | N-succinyl-Ala-Ala-Pro-Phe p-nitroanilide10 mM DTT in assay | — | — | 189 | pH 7.5, 40 °C | Open record: Proteolysin PrlA variant C182A/C201A, N-succinyl-Ala-Ala-Pro-Phe p-nitroanilide |
| Proteolysin PrlAvariant C182A/C201A | Coprothermobacter proteolyticus | N-succinyl-Ala-Ala-Pro-Phe p-nitroanilide | 248 | 1.96 | 126 | pH 7.5, 70 °C | Open record: Proteolysin PrlA variant C182A/C201A, N-succinyl-Ala-Ala-Pro-Phe p-nitroanilide |
| Subtilisin A | — | N-succinyl-Ala-Ala-Pro-Phe p-nitroanilide | 551 | — | — | pH 7.5, 40 °C | Open record: Subtilisin A, N-succinyl-Ala-Ala-Pro-Phe p-nitroanilide |
| Subtilisin A | — | N-succinyl-Ala-Ala-Pro-Phe p-nitroanilide | — | 1.1 | — | pH 7.5, 40 °C | Open record: Subtilisin A, N-succinyl-Ala-Ala-Pro-Phe p-nitroanilide |
| Subtilisin A | — | N-succinyl-Ala-Ala-Pro-Phe p-nitroanilide | — | — | 489 | pH 7.5, 40 °C | Open record: Subtilisin A, N-succinyl-Ala-Ala-Pro-Phe p-nitroanilide |
| Subtilisin A | — | N-succinyl-Ala-Ala-Pro-Phe p-nitroanilide10 mM DTT in assay | 40 | — | — | pH 7.5, 40 °C | Open record: Subtilisin A, N-succinyl-Ala-Ala-Pro-Phe p-nitroanilide |
| Subtilisin A | — | N-succinyl-Ala-Ala-Pro-Phe p-nitroanilide10 mM DTT in assay | — | 1.57 | — | pH 7.5, 40 °C | Open record: Subtilisin A, N-succinyl-Ala-Ala-Pro-Phe p-nitroanilide |
| Subtilisin A | — | N-succinyl-Ala-Ala-Pro-Phe p-nitroanilide10 mM DTT in assay | — | — | 23 | pH 7.5, 40 °C | Open record: Subtilisin A, N-succinyl-Ala-Ala-Pro-Phe p-nitroanilide |
| Subtilisin A | — | N-succinyl-Ala-Ala-Pro-Phe p-nitroanilide | 539 | 1.06 | 508 | pH 7.5, 70 °C | Open record: Subtilisin A, N-succinyl-Ala-Ala-Pro-Phe p-nitroanilide |