CatLogenzyme kinetics catalog

EC 3.4.21.89: Signal peptidase I

HydrolasesOpen in Browse

CatLog read 31 records for EC 3.4.21.89 from 2 papers, for 2 organisms. Names in these papers: signal peptidase I (leader peptidase, SPase I), signal peptide peptidase SppA_Tk (ΔN54SppA_Tk).

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units.

CatLog also holds 19 records for EC 3.4.21.89 that are not yet re-read: from its earlier version, built from the enzyme databases and partly checked against the papers. All records for EC 3.4.21.89 in Browse.

Papers

PaperOrganismsRecords
Identification of arginine residues important for the activity of Escherichia coli signal peptidase IEscherichia coli18
Identification of the amino acid residues essential for proteolytic activity in an archaeal signal peptide peptidaseThermococcus kodakaraensis13

Records

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)ConditionsPaper
signal peptidase I (leader peptidase, SPase I)Escherichia coliFSASALAKI0.0240.520.04617pH 7.7, 37 °CPMID 15195997Open
signal peptidase I (leader peptidase, SPase I)variant R127AEscherichia coliFSASALAKI0.018440.490.03764pH 7.7, 37 °CPMID 15195997Open
signal peptidase I (leader peptidase, SPase I)variant R146AEscherichia coliFSASALAKI0.0240.520.04617pH 7.7, 37 °CPMID 15195997Open
signal peptidase I (leader peptidase, SPase I)variant R198AEscherichia coliFSASALAKI0.018440.520.03547pH 7.7, 37 °CPMID 15195997Open
signal peptidase I (leader peptidase, SPase I)variant R199AEscherichia coliFSASALAKI0.02180.550.03964pH 7.7, 37 °CPMID 15195997Open
signal peptidase I (leader peptidase, SPase I)variant R222AEscherichia coliFSASALAKI0.004530.540.00839pH 7.7, 37 °CPMID 15195997Open
signal peptidase I (leader peptidase, SPase I)variant R222KEscherichia coliFSASALAKI0.005280.530.00994pH 7.7, 37 °CPMID 15195997Open
signal peptidase I (leader peptidase, SPase I)variant R226AEscherichia coliFSASALAKI0.0160.520.03078pH 7.7, 37 °CPMID 15195997Open
signal peptidase I (leader peptidase, SPase I)variant R236AEscherichia coliFSASALAKI0.02180.520.04542pH 7.7, 37 °CPMID 15195997Open
signal peptidase I (leader peptidase, SPase I)variant R275AEscherichia coliFSASALAKI0.02180.490.0445pH 7.7, 37 °CPMID 15195997Open
signal peptidase I (leader peptidase, SPase I)variant R282AEscherichia coliFSASALAKI0.020.490.04081pH 7.7, 37 °CPMID 15195997Open
signal peptidase I (leader peptidase, SPase I)variant R295AEscherichia coliFSASALAKI0.020.520.03847pH 7.7, 37 °CPMID 15195997Open
signal peptidase I (leader peptidase, SPase I)variant R315AEscherichia coliFSASALAKI0.007060.570.0124pH 7.7, 37 °CPMID 15195997Open
signal peptidase I (leader peptidase, SPase I)variant R315KEscherichia coliFSASALAKI0.0170.490.03469pH 7.7, 37 °CPMID 15195997Open
signal peptidase I (leader peptidase, SPase I)variant R318AEscherichia coliFSASALAKI0.001330.430.00311pH 7.7, 37 °CPMID 15195997Open
signal peptidase I (leader peptidase, SPase I)variant R318KEscherichia coliFSASALAKI6.1 × 10⁻⁴0.530.00117pH 7.7, 37 °CPMID 15195997Open
signal peptidase I (leader peptidase, SPase I)variant R77AEscherichia coliFSASALAKI0.004140.670.00617pH 7.7, 37 °CPMID 15195997Open
signal peptidase I (leader peptidase, SPase I)variant R77KEscherichia coliFSASALAKI0.01190.630.01886pH 7.7, 37 °CPMID 15195997Open
signal peptide peptidase SppA_Tk (ΔN54SppA_Tk)Thermococcus kodakaraensisAla-Ala-Phe-4-methylcoumaryl-7-amide160.32549pH 10, 60 °CPMID 16484219Open
signal peptide peptidase SppA_Tk (ΔN54SppA_Tk)variant D215AThermococcus kodakaraensisAla-Ala-Phe-4-methylcoumaryl-7-amide8.81.197.4pH 10, 60 °CPMID 16484219Open
signal peptide peptidase SppA_Tk (ΔN54SppA_Tk)variant D277AThermococcus kodakaraensisAla-Ala-Phe-4-methylcoumaryl-7-amide250.4457pH 10, 60 °CPMID 16484219Open
signal peptide peptidase SppA_Tk (ΔN54SppA_Tk)variant E226AThermococcus kodakaraensisAla-Ala-Phe-4-methylcoumaryl-7-amide210.32666pH 10, 60 °CPMID 16484219Open
signal peptide peptidase SppA_Tk (ΔN54SppA_Tk)variant E227AThermococcus kodakaraensisAla-Ala-Phe-4-methylcoumaryl-7-amide161.4211pH 10, 60 °CPMID 16484219Open
signal peptide peptidase SppA_Tk (ΔN54SppA_Tk)variant H191AThermococcus kodakaraensisAla-Ala-Phe-4-methylcoumaryl-7-amide8.60.37323pH 10, 60 °CPMID 16484219Open
signal peptide peptidase SppA_Tk (ΔN54SppA_Tk)variant K150AThermococcus kodakaraensisAla-Ala-Phe-4-methylcoumaryl-7-amide230.29380pH 10, 60 °CPMID 16484219Open
signal peptide peptidase SppA_Tk (ΔN54SppA_Tk)variant K209AThermococcus kodakaraensisAla-Ala-Phe-4-methylcoumaryl-7-amide9.10.30730pH 10, 60 °CPMID 16484219Open
signal peptide peptidase SppA_Tk (ΔN54SppA_Tk)variant R221AThermococcus kodakaraensisAla-Ala-Phe-4-methylcoumaryl-7-amide21.761.2pH 10, 60 °CPMID 16484219Open
signal peptide peptidase SppA_Tk (ΔN54SppA_Tk)variant R250AThermococcus kodakaraensisAla-Ala-Phe-4-methylcoumaryl-7-amide230.27883pH 10, 60 °CPMID 16484219Open
signal peptide peptidase SppA_Tk (ΔN54SppA_Tk)variant S128AThermococcus kodakaraensisAla-Ala-Phe-4-methylcoumaryl-7-amide1101.4376pH 10, 60 °CPMID 16484219Open
signal peptide peptidase SppA_Tk (ΔN54SppA_Tk)variant S184AThermococcus kodakaraensisAla-Ala-Phe-4-methylcoumaryl-7-amide40.36611pH 10, 60 °CPMID 16484219Open
signal peptide peptidase SppA_Tk (ΔN54SppA_Tk)variant Y165AThermococcus kodakaraensisAla-Ala-Phe-4-methylcoumaryl-7-amide370.346110pH 10, 60 °CPMID 16484219Open