Values CatLog read from this paper
Identification of the amino acid residues essential for proteolytic activity in an archaeal signal peptide peptidase
PMID 16484219doi:10.1074/jbc.m513754200Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 13 records (39 values) from this paper: signal peptide peptidase SppA_Tk (ΔN54SppA_Tk) from Thermococcus kodakaraensis.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| signal peptide peptidase SppA_Tk (ΔN54SppA_Tk) | Thermococcus kodakaraensis | Ala-Ala-Phe-4-methylcoumaryl-7-amide | 16 | 0.325 | 49 | pH 10, 60 °C | Open record: signal peptide peptidase SppA_Tk (ΔN54SppA_Tk), Ala-Ala-Phe-4-methylcoumaryl-7-amide |
| signal peptide peptidase SppA_Tk (ΔN54SppA_Tk)variant D215A | Thermococcus kodakaraensis | Ala-Ala-Phe-4-methylcoumaryl-7-amide | 8.8 | 1.19 | 7.4 | pH 10, 60 °C | Open record: signal peptide peptidase SppA_Tk (ΔN54SppA_Tk) variant D215A, Ala-Ala-Phe-4-methylcoumaryl-7-amide |
| signal peptide peptidase SppA_Tk (ΔN54SppA_Tk)variant D277A | Thermococcus kodakaraensis | Ala-Ala-Phe-4-methylcoumaryl-7-amide | 25 | 0.44 | 57 | pH 10, 60 °C | Open record: signal peptide peptidase SppA_Tk (ΔN54SppA_Tk) variant D277A, Ala-Ala-Phe-4-methylcoumaryl-7-amide |
| signal peptide peptidase SppA_Tk (ΔN54SppA_Tk)variant E226A | Thermococcus kodakaraensis | Ala-Ala-Phe-4-methylcoumaryl-7-amide | 21 | 0.326 | 66 | pH 10, 60 °C | Open record: signal peptide peptidase SppA_Tk (ΔN54SppA_Tk) variant E226A, Ala-Ala-Phe-4-methylcoumaryl-7-amide |
| signal peptide peptidase SppA_Tk (ΔN54SppA_Tk)variant E227A | Thermococcus kodakaraensis | Ala-Ala-Phe-4-methylcoumaryl-7-amide | 16 | 1.42 | 11 | pH 10, 60 °C | Open record: signal peptide peptidase SppA_Tk (ΔN54SppA_Tk) variant E227A, Ala-Ala-Phe-4-methylcoumaryl-7-amide |
| signal peptide peptidase SppA_Tk (ΔN54SppA_Tk)variant H191A | Thermococcus kodakaraensis | Ala-Ala-Phe-4-methylcoumaryl-7-amide | 8.6 | 0.373 | 23 | pH 10, 60 °C | Open record: signal peptide peptidase SppA_Tk (ΔN54SppA_Tk) variant H191A, Ala-Ala-Phe-4-methylcoumaryl-7-amide |
| signal peptide peptidase SppA_Tk (ΔN54SppA_Tk)variant K150A | Thermococcus kodakaraensis | Ala-Ala-Phe-4-methylcoumaryl-7-amide | 23 | 0.293 | 80 | pH 10, 60 °C | Open record: signal peptide peptidase SppA_Tk (ΔN54SppA_Tk) variant K150A, Ala-Ala-Phe-4-methylcoumaryl-7-amide |
| signal peptide peptidase SppA_Tk (ΔN54SppA_Tk)variant K209A | Thermococcus kodakaraensis | Ala-Ala-Phe-4-methylcoumaryl-7-amide | 9.1 | 0.307 | 30 | pH 10, 60 °C | Open record: signal peptide peptidase SppA_Tk (ΔN54SppA_Tk) variant K209A, Ala-Ala-Phe-4-methylcoumaryl-7-amide |
| signal peptide peptidase SppA_Tk (ΔN54SppA_Tk)variant R221A | Thermococcus kodakaraensis | Ala-Ala-Phe-4-methylcoumaryl-7-amide | 2 | 1.76 | 1.2 | pH 10, 60 °C | Open record: signal peptide peptidase SppA_Tk (ΔN54SppA_Tk) variant R221A, Ala-Ala-Phe-4-methylcoumaryl-7-amide |
| signal peptide peptidase SppA_Tk (ΔN54SppA_Tk)variant R250A | Thermococcus kodakaraensis | Ala-Ala-Phe-4-methylcoumaryl-7-amide | 23 | 0.278 | 83 | pH 10, 60 °C | Open record: signal peptide peptidase SppA_Tk (ΔN54SppA_Tk) variant R250A, Ala-Ala-Phe-4-methylcoumaryl-7-amide |
| signal peptide peptidase SppA_Tk (ΔN54SppA_Tk)variant S128A | Thermococcus kodakaraensis | Ala-Ala-Phe-4-methylcoumaryl-7-amide | 110 | 1.43 | 76 | pH 10, 60 °C | Open record: signal peptide peptidase SppA_Tk (ΔN54SppA_Tk) variant S128A, Ala-Ala-Phe-4-methylcoumaryl-7-amide |
| signal peptide peptidase SppA_Tk (ΔN54SppA_Tk)variant S184A | Thermococcus kodakaraensis | Ala-Ala-Phe-4-methylcoumaryl-7-amide | 4 | 0.366 | 11 | pH 10, 60 °C | Open record: signal peptide peptidase SppA_Tk (ΔN54SppA_Tk) variant S184A, Ala-Ala-Phe-4-methylcoumaryl-7-amide |
| signal peptide peptidase SppA_Tk (ΔN54SppA_Tk)variant Y165A | Thermococcus kodakaraensis | Ala-Ala-Phe-4-methylcoumaryl-7-amide | 37 | 0.346 | 110 | pH 10, 60 °C | Open record: signal peptide peptidase SppA_Tk (ΔN54SppA_Tk) variant Y165A, Ala-Ala-Phe-4-methylcoumaryl-7-amide |