CatLogenzyme kinetics catalog

EC 3.4.21.91: Flavivirin

HydrolasesOpen in Browse

CatLog read 154 records for EC 3.4.21.91 from 5 papers, for 9 organisms. Names in these papers: NS2B-NS3 protease, dengue 2 virus NS2B-NS3 protease (CF40glyNS3pro), NS2B/NS3 protease, ΔNS2B-NS3pro (NS2B/NS3 protease complex) and 1 more.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units.

CatLog also holds 34 records for EC 3.4.21.91 that are not yet re-read: from its earlier version, built from the enzyme databases and partly checked against the papers. All records for EC 3.4.21.91 in Browse.

Papers

PaperOrganismsRecords
Insights to substrate binding and processing by West Nile Virus NS3 protease through combined modeling, protease mutagenesis, and kinetic studiesWest Nile virus71
Substrate specificity of recombinant dengue 2 virus NS2B-NS3 protease: influence of natural and unnatural basic amino acids on hydrolysis of synthetic fluorescent substratesDengue virus 2, dengue 2 virus46
Yellow fever virus NS2B/NS3 protease: hydrolytic properties and substrate specificityYellow fever virus21
Functional characterization of the NS2B/NS3 protease complex from seven viruses belonging to different groups inside the genus FlavivirusBussuquara virus, Dengue virus type 3 and 4 more11
Extended substrate specificity and first potent irreversible inhibitor/activity-based probe design for Zika virus NS2B-NS3 proteaseZika virus5

Records

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)ConditionsPaper
dengue 2 virus NS2B-NS3 protease (CF40glyNS3pro)variant strain BR/01-EN (NS3_{BR/01})dengue 2 virusAbz–EVKKQRAGVLWDQ–EDDnp (peptide 2)——1.6pH 9, 37 °CPMID 17184724Open
dengue 2 virus NS2B-NS3 protease (CF40glyNS3pro)variant strain BR/01-EN (NS3_{BR/01})dengue 2 virusAbz–HRREKRSVALQ–EDDnp (prM/M* site peptide)——0.8pH 9, 37 °CPMID 17184724Open
dengue 2 virus NS2B-NS3 protease (CF40glyNS3pro)variant strain BR/01-EN (NS3_{BR/01})dengue 2 virusAbz–LNRRRRTAGMIIQ–EDDnp (peptide 5)——5.1pH 9, 37 °CPMID 17184724Open
dengue 2 virus NS2B-NS3 protease (CF40glyNS3pro)variant strain BR/01-EN (NS3_{BR/01})dengue 2 virusAbz–RTSKKRSWPLNEQ–EDDnp (peptide 1)——2.9pH 9, 37 °CPMID 17184724Open
dengue 2 virus NS2B-NS3 protease (CF40glyNS3pro)variant strain BR/01-EN (NS3_{BR/01})dengue 2 virusAbz–SAAQRRGRIGRNQ–EDDnp (peptide 6)——15.8pH 9, 37 °CPMID 17184724Open
dengue 2 virus NS2B-NS3 protease (CF40glyNS3pro)variant strain BR/01-EN (NS3_{BR/01})dengue 2 virusAbz–TTSTRRGTGNIGQ–EDDnp (peptide 4)——0.7pH 9, 37 °CPMID 17184724Open
dengue 2 virus NS2B-NS3 protease (CF40glyNS3pro)variant strain NGC (NS3_{NGC})dengue 2 virusAbz–AGKKSAQ–EDDnp (peptide 18)0.020.05880.3pH 9, 37 °CPMID 17184724Open
dengue 2 virus NS2B-NS3 protease (CF40glyNS3pro)variant strain NGC (NS3_{NGC})dengue 2 virusAbz–AGKRSAQ–EDDnp (peptide 20)0.20.0385.3pH 9, 37 °CPMID 17184724Open
dengue 2 virus NS2B-NS3 protease (CF40glyNS3pro)variant strain NGC (NS3_{NGC})dengue 2 virusAbz–AGRKSAQ–EDDnp (peptide 19)0.130.02136.1pH 9, 37 °CPMID 17184724Open
dengue 2 virus NS2B-NS3 protease (CF40glyNS3pro)variant strain NGC (NS3_{NGC})dengue 2 virusAbz–AGRRSAQ–EDDnp (peptide 17)0.210.0210.5pH 9, 37 °CPMID 17184724Open
dengue 2 virus NS2B-NS3 protease (CF40glyNS3pro)variant strain NGC (NS3_{NGC})dengue 2 virusAbz–AKKKSQ–EDDnp (peptide 26)0.040.06920.6pH 9, 37 °CPMID 17184724Open
dengue 2 virus NS2B-NS3 protease (CF40glyNS3pro)variant strain NGC (NS3_{NGC})dengue 2 virusAbz–AKKRSQ–EDDnp (peptide 28)0.070.05321.3pH 9, 37 °CPMID 17184724Open
dengue 2 virus NS2B-NS3 protease (CF40glyNS3pro)variant strain NGC (NS3_{NGC})dengue 2 virusAbz–AKRKSQ–EDDnp (peptide 27)0.040.02371.7pH 9, 37 °CPMID 17184724Open
dengue 2 virus NS2B-NS3 protease (CF40glyNS3pro)variant strain NGC (NS3_{NGC})dengue 2 virusAbz–AKRRSQ–EDDnp (peptide 22)10.031431.8pH 9, 37 °CPMID 17184724Open
dengue 2 virus NS2B-NS3 protease (CF40glyNS3pro)variant strain NGC (NS3_{NGC})dengue 2 virusAbz–ARKKSQ–EDDnp (peptide 25)0.050.06730.7pH 9, 37 °CPMID 17184724Open
dengue 2 virus NS2B-NS3 protease (CF40glyNS3pro)variant strain NGC (NS3_{NGC})dengue 2 virusAbz–ARKRSQ–EDDnp (peptide 23)0.070.0581.2pH 9, 37 °CPMID 17184724Open
dengue 2 virus NS2B-NS3 protease (CF40glyNS3pro)variant strain NGC (NS3_{NGC})dengue 2 virusAbz–ARRKSQ–EDDnp (peptide 24)0.090.01834.9pH 9, 37 °CPMID 17184724Open
dengue 2 virus NS2B-NS3 protease (CF40glyNS3pro)variant strain NGC (NS3_{NGC})dengue 2 virusAbz–ARRRSQ–EDDnp (peptide 21)0.380.025614.8pH 9, 37 °CPMID 17184724Open
dengue 2 virus NS2B-NS3 protease (CF40glyNS3pro)variant strain NGC (NS3_{NGC})dengue 2 virusAbz–EVKKQRAGVLWDQ–EDDnp (peptide 2)0.030.00883.4pH 9, 37 °CPMID 17184724Open
dengue 2 virus NS2B-NS3 protease (CF40glyNS3pro)variant strain NGC (NS3_{NGC})dengue 2 virusAbz–FAAGRKSLTLNLQ–EDDnp (peptide 3)0.020.01771.2pH 9, 37 °CPMID 17184724Open
dengue 2 virus NS2B-NS3 protease (CF40glyNS3pro)variant strain NGC (NS3_{NGC})dengue 2 virusAbz–FAAGRRSLTLNLQ–EDDnp0.620.01255pH 9, 37 °CPMID 17184724Open
dengue 2 virus NS2B-NS3 protease (CF40glyNS3pro)variant strain NGC (NS3_{NGC})dengue 2 virusAbz–FAAQRRGRIGRNQ–EDDnp (peptide 8)0.140.007419.4pH 9, 37 °CPMID 17184724Open
dengue 2 virus NS2B-NS3 protease (CF40glyNS3pro)variant strain NGC (NS3_{NGC})dengue 2 virusAbz–HRREKRSVALQ–EDDnp (prM/M* site peptide)0.040.0261.5pH 9, 37 °CPMID 17184724Open
dengue 2 virus NS2B-NS3 protease (CF40glyNS3pro)variant strain NGC (NS3_{NGC})dengue 2 virusAbz–LNRRRRTAGMIIQ–EDDnp (peptide 5)0.040.003910.2pH 9, 37 °CPMID 17184724Open
dengue 2 virus NS2B-NS3 protease (CF40glyNS3pro)variant strain NGC (NS3_{NGC})dengue 2 virusAbz–RTSKKRSWPLNEQ–EDDnp (peptide 1)FRET assay; fluorescence 420 nm with excitation at 320 nm0.170.035.7pH 9, 37 °CPMID 17184724Open
dengue 2 virus NS2B-NS3 protease (CF40glyNS3pro)variant strain NGC (NS3_{NGC})dengue 2 virusAbz–RTSKKRSWPLNEQ–EDDnp (peptide 1)37 °C; reaction stopped with 0.33% trifluoroacetic acid—0.0391—pH 9, 37 °CPMID 17184724Open
dengue 2 virus NS2B-NS3 protease (CF40glyNS3pro)variant strain NGC (NS3_{NGC})dengue 2 virusAbz–SAAGRRGRIGRNQ–EDDnp (peptide 9)0.090.00422pH 9, 37 °CPMID 17184724Open
dengue 2 virus NS2B-NS3 protease (CF40glyNS3pro)variant strain NGC (NS3_{NGC})dengue 2 virusAbz–SAAQRKGRIGRNQ–EDDnp (peptide 10)0.040.00984.6pH 9, 37 °CPMID 17184724Open
dengue 2 virus NS2B-NS3 protease (CF40glyNS3pro)variant strain NGC (NS3_{NGC})dengue 2 virusAbz–SAAQRRGLIGRNQ–EDDnp (peptide 12)0.110.007215.5pH 9, 37 °CPMID 17184724Open
dengue 2 virus NS2B-NS3 protease (CF40glyNS3pro)variant strain NGC (NS3_{NGC})dengue 2 virusAbz–SAAQRRGRIGNNQ–EDDnp (peptide 15)0.110.004623.1pH 9, 37 °CPMID 17184724Open
dengue 2 virus NS2B-NS3 protease (CF40glyNS3pro)variant strain NGC (NS3_{NGC})dengue 2 virusAbz–SAAQRRGRIGRLQ–EDDnp (peptide 16)0.160.004535.9pH 9, 37 °CPMID 17184724Open
dengue 2 virus NS2B-NS3 protease (CF40glyNS3pro)variant strain NGC (NS3_{NGC})dengue 2 virusAbz–SAAQRRGRIGRNQ–EDDnp (peptide 6)0.290.013821pH 9, 37 °CPMID 17184724Open
dengue 2 virus NS2B-NS3 protease (CF40glyNS3pro)variant strain NGC (NS3_{NGC})dengue 2 virusAbz–SAAQRRGRILRNQ–EDDnp (peptide 14)0.180.006926.5pH 9, 37 °CPMID 17184724Open
dengue 2 virus NS2B-NS3 protease (CF40glyNS3pro)variant strain NGC (NS3_{NGC})dengue 2 virusAbz–SAAQRRGRTGRNQ–EDDnp (peptide 13)0.340.016520.9pH 9, 37 °CPMID 17184724Open
dengue 2 virus NS2B-NS3 protease (CF40glyNS3pro)variant strain NGC (NS3_{NGC})dengue 2 virusAbz–SAAQRRSRIGRNQ–EDDnp (peptide 11)0.140.005923.5pH 9, 37 °CPMID 17184724Open
dengue 2 virus NS2B-NS3 protease (CF40glyNS3pro)variant strain NGC (NS3_{NGC})dengue 2 virusAbz–TTSTRRGTGNIGQ–EDDnp (peptide 4)0.040.0271.5pH 9, 37 °CPMID 17184724Open
dengue 2 virus NS2B-NS3 protease (CF40glyNS3pro)variant strain NGC (NS3_{NGC})dengue 2 virusBz–(3-pyridyl-alanine)–Arg–MCA, Bz = benzoyl group0.0020.0430.05pH 9, 37 °CPMID 17184724Open
dengue 2 virus NS2B-NS3 protease (CF40glyNS3pro)variant strain NGC (NS3_{NGC})dengue 2 virusBz–(4-aminocyclohexyl-alanine)–Arg–MCA, Bz = benzoyl group0.0130.0270.48pH 9, 37 °CPMID 17184724Open
dengue 2 virus NS2B-NS3 protease (CF40glyNS3pro)variant strain NGC (NS3_{NGC})dengue 2 virusBz–(4-aminomethyl-cyclohexyl-alanine)–Arg–MCA, Bz = benzoyl group0.1720.2980.58pH 9, 37 °CPMID 17184724Open
dengue 2 virus NS2B-NS3 protease (CF40glyNS3pro)variant strain NGC (NS3_{NGC})dengue 2 virusBz–(4-aminomethyl-N-isopropyl-phenylalanine)–Arg–MCA, Bz = benzoyl group0.0220.3540.06pH 9, 37 °CPMID 17184724Open
dengue 2 virus NS2B-NS3 protease (CF40glyNS3pro)variant strain NGC (NS3_{NGC})dengue 2 virusBz–(4-aminomethyl-phenylalanine)–Arg–MCA, Bz = benzoyl group0.0130.0260.5pH 9, 37 °CPMID 17184724Open
dengue 2 virus NS2B-NS3 protease (CF40glyNS3pro)variant strain NGC (NS3_{NGC})dengue 2 virusBz–(4-guanidine phenylalanine)–Arg–MCA, Bz = benzoyl group0.0350.490.07pH 9, 37 °CPMID 17184724Open
dengue 2 virus NS2B-NS3 protease (CF40glyNS3pro)variant strain NGC (NS3_{NGC})dengue 2 virusBz–(4-piperidinyl-alanine)–Arg–MCA, Bz = benzoyl group0.0140.2160.07pH 9, 37 °CPMID 17184724Open
dengue 2 virus NS2B-NS3 protease (CF40glyNS3pro)variant strain NGC (NS3_{NGC})dengue 2 virusBz–Arg–Arg–MCA (peptide 31)0.1090.2470.44pH 9, 37 °CPMID 17184724Open
dengue 2 virus NS2B-NS3 protease (CF40glyNS3pro), strain NGCvariant CF40glyNS3pro, dengue 2 virus strain NGCDengue virus 2Abz–RTSKKRSWPLNEQ–EDDnp—0.03—pH 9, 37 °CPMID 17184724Open
dengue 2 virus NS2B-NS3 protease (CF40glyNS3pro), strain NGCvariant CF40glyNS3pro, dengue 2 virus strain NGCDengue virus 2Z–RR–MCA0.110.2470.5pH 9, 37 °CPMID 17184724Open
NS2B-NS3 proteaseWest Nile virus2-Naph-Lys-AcLys-Arg-pNA0.32.3030.131pH 9.5, 37 °CPMID 17052977Open
NS2B-NS3 proteaseWest Nile virus2-naphthoyl-3-pyridylalanyl-L-lysyl-L-arginine para-nitroanilide0.960.065814.62pH 9.5, 37 °CPMID 17052977Open
NS2B-NS3 proteaseWest Nile virus2-naphthoyl-L-citrullyl-L-lysyl-L-arginine para-nitroanilide0.320.02115.15pH 9.5, 37 °CPMID 17052977Open
NS2B-NS3 proteaseWest Nile virus2-naphthoyl-L-citrullyl-L-ornithyl-L-arginine para-nitroanilide0.570.10075.612pH 9.5, 37 °CPMID 17052977Open
NS2B-NS3 proteaseWest Nile virus2-naphthoyl-L-homoarginyl-L-lysyl-L-arginine para-nitroanilide0.110.0482.294pH 9.5, 37 °CPMID 17052977Open
NS2B-NS3 proteaseWest Nile virus2-naphthoyl-L-lysyl-L-arginyl-L-arginine para-nitroanilide0.8850.043420.38pH 9.5, 37 °CPMID 17052977Open
NS2B-NS3 proteaseWest Nile virus2-naphthoyl-L-lysyl-L-citrullyl-L-arginine para-nitroanilide0.433.4230.127pH 9.5, 37 °CPMID 17052977Open
NS2B-NS3 proteaseWest Nile virus2-naphthoyl-L-lysyl-L-homoarginyl-L-arginine para-nitroanilide0.590.026722.1pH 9.5, 37 °CPMID 17052977Open
NS2B-NS3 proteaseWest Nile virus2-naphthoyl-L-lysyl-L-lysyl-L-arginine para-nitroanilide1.0830.025442.60pH 9.5, 37 °CPMID 17052977Open
NS2B-NS3 proteaseWest Nile virus2-naphthoyl-L-lysyl-L-ornithyl-L-arginine para-nitroanilide1.580.036842.89pH 9.5, 37 °CPMID 17052977Open
NS2B-NS3 proteaseWest Nile virus2-naphthoyl-L-ornithyl-L-lysyl-L-arginine para-nitroanilide0.980.03825.74pH 9.5, 37 °CPMID 17052977Open
NS2B-NS3 proteaseWest Nile virus2-naphthoyl-L-ornithyl-L-ornithyl-L-arginine para-nitroanilide0.270.022911.90pH 9.5, 37 °CPMID 17052977Open
NS2B-NS3 proteaseWest Nile virusN-acetyl-2-aminoisobutyryl-L-lysyl-L-lysyl-L-arginine para-nitroanilide1.60.079720.05pH 9.5, 37 °CPMID 17052977Open
NS2B-NS3 proteaseWest Nile virusN-acetyl-L-2-aminooctanoyl-L-lysyl-L-lysyl-L-arginine para-nitroanilide2.510.075533.19pH 9.5, 37 °CPMID 17052977Open
NS2B-NS3 proteaseWest Nile virusN-acetyl-L-cyclohexylalanyl-L-lysyl-L-lysyl-L-arginine para-nitroanilide2.280.069932.60pH 9.5, 37 °CPMID 17052977Open
NS2B-NS3 proteaseWest Nile virusN-acetyl-L-cyclohexylglycyl-L-lysyl-L-lysyl-L-arginine para-nitroanilide1.780.081921.73pH 9.5, 37 °CPMID 17052977Open
NS2B-NS3 proteaseWest Nile virusN-acetyl-L-leucyl-3-pyridylalanyl-L-lysyl-L-arginine para-nitroanilide0.50.08875.601pH 9.5, 37 °CPMID 17052977Open
NS2B-NS3 proteaseWest Nile virusN-acetyl-L-leucyl-4-amino-L-phenylalanyl-L-lysyl-L-arginine para-nitroanilide0.750.14135.306pH 9.5, 37 °CPMID 17052977Open
NS2B-NS3 proteaseWest Nile virusN-acetyl-L-leucyl-4-nitro-L-phenylalanyl-L-lysyl-L-arginine para-nitroanilide0.720.25412.817pH 9.5, 37 °CPMID 17052977Open
NS2B-NS3 proteaseWest Nile virusN-acetyl-L-leucyl-4-pyridylalanyl-L-lysyl-L-arginine para-nitroanilide0.90.25483.536pH 9.5, 37 °CPMID 17052977Open
NS2B-NS3 proteaseWest Nile virusN-acetyl-L-leucyl-L-citrullyl-L-lysyl-L-arginine para-nitroanilide1.440.20866.904pH 9.5, 37 °CPMID 17052977Open
NS2B-NS3 proteaseWest Nile virusN-acetyl-L-leucyl-L-glutaminyl-L-tyrosyl-L-threonyl-L-lysyl-L-arginine para-nitroanilide0.2660.1991.332pH 9.5, 37 °CPMID 17052977Open
NS2B-NS3 proteaseWest Nile virusN-acetyl-L-leucyl-L-homoarginyl-L-lysyl-L-arginine para-nitroanilide1.180.062518.96pH 9.5, 37 °CPMID 17052977Open
NS2B-NS3 proteaseWest Nile virusN-acetyl-L-leucyl-L-lysyl-L-arginyl-L-arginine para-nitroanilide2.4940.19512.78pH 9.5, 37 °CPMID 17052977Open
NS2B-NS3 proteaseWest Nile virusN-acetyl-L-leucyl-L-lysyl-L-lysyl-L-arginine para-nitroanilide1.6440.054630.12pH 9.5, 37 °CPMID 17052977Open
NS2B-NS3 proteaseWest Nile virusN-acetyl-L-leucyl-L-ornithyl-L-lysyl-L-arginine para-nitroanilide1.790.105517.00pH 9.5, 37 °CPMID 17052977Open
NS2B-NS3 proteaseWest Nile virusN-acetyl-L-norleucyl-L-lysyl-L-lysyl-L-arginine para-nitroanilide2.350.080729.09pH 9.5, 37 °CPMID 17052977Open
NS2B-NS3 proteaseWest Nile virusN-acetyl-L-norvalyl-L-lysyl-L-lysyl-L-arginine para-nitroanilide2.360.067335.00pH 9.5, 37 °CPMID 17052977Open
NS2B-NS3 proteaseWest Nile virusN-acetyl-L-tert-butylglycyl-L-lysyl-L-lysyl-L-arginine para-nitroanilide1.740.134212.96pH 9.5, 37 °CPMID 17052977Open
NS2B-NS3 proteasevariant NS2B-L87AWest Nile virus2-naphthoyl-L-lysyl-L-lysyl-L-arginine para-nitroanilide0.04780.08840.541pH 9.5, 37 °CPMID 17052977Open
NS2B-NS3 proteasevariant NS2B-L87AWest Nile virusN-acetyl-L-leucyl-L-lysyl-L-lysyl-L-arginine para-nitroanilide0.070.4340.161pH 9.5, 37 °CPMID 17052977Open
NS2B-NS3 proteasevariant NS2B-L87FWest Nile virus2-naphthoyl-L-lysyl-L-lysyl-L-arginine para-nitroanilide0.2880.03697.796pH 9.5, 37 °CPMID 17052977Open
NS2B-NS3 proteasevariant NS2B-L87FWest Nile virusN-acetyl-L-leucyl-L-glutaminyl-L-tyrosyl-L-threonyl-L-lysyl-L-arginine para-nitroanilide0.2280.7640.299pH 9.5, 37 °CPMID 17052977Open
NS2B-NS3 proteasevariant NS2B-L87FWest Nile virusN-acetyl-L-leucyl-L-lysyl-L-lysyl-L-arginine para-nitroanilide1.3420.1449.345pH 9.5, 37 °CPMID 17052977Open
NS2B-NS3 proteasevariant NS2B-N84AWest Nile virusN-acetyl-L-leucyl-L-lysyl-L-lysyl-L-arginine para-nitroanilide0.1260.3760.336pH 9.5, 37 °CPMID 17052977Open
NS2B-NS3 proteasevariant NS2B-N84DWest Nile virusN-acetyl-L-leucyl-L-lysyl-L-arginyl-L-arginine para-nitroanilide0.3980.06985.698pH 9.5, 37 °CPMID 17052977Open
NS2B-NS3 proteasevariant NS2B-N84DWest Nile virusN-acetyl-L-leucyl-L-lysyl-L-lysyl-L-arginine para-nitroanilide1.5320.11713.11pH 9.5, 37 °CPMID 17052977Open
NS2B-NS3 proteasevariant NS2B-N84EWest Nile virusN-acetyl-L-leucyl-L-lysyl-L-arginyl-L-arginine para-nitroanilide2.7070.066440.80pH 9.5, 37 °CPMID 17052977Open
NS2B-NS3 proteasevariant NS2B-N84EWest Nile virusN-acetyl-L-leucyl-L-lysyl-L-lysyl-L-arginine para-nitroanilide3.0270.079338.19pH 9.5, 37 °CPMID 17052977Open
NS2B-NS3 proteasevariant NS2B-N84LWest Nile virusN-acetyl-L-leucyl-L-lysyl-L-lysyl-L-arginine para-nitroanilide0.340.221.543pH 9.5, 37 °CPMID 17052977Open
NS2B-NS3 proteasevariant NS2B-N84SWest Nile virus2-naphthoyl-L-lysyl-L-arginyl-L-arginine para-nitroanilide0.1510.01977.695pH 9.5, 37 °CPMID 17052977Open
NS2B-NS3 proteasevariant NS2B-N84SWest Nile virus2-naphthoyl-L-lysyl-L-homoarginyl-L-arginine para-nitroanilide0.0270.02950.904pH 9.5, 37 °CPMID 17052977Open
NS2B-NS3 proteasevariant NS2B-N84SWest Nile virusN-acetyl-L-leucyl-L-lysyl-L-arginyl-L-arginine para-nitroanilide0.1580.11.577pH 9.5, 37 °CPMID 17052977Open
NS2B-NS3 proteasevariant NS2B-N84SWest Nile virusN-acetyl-L-leucyl-L-lysyl-L-lysyl-L-arginine para-nitroanilide0.6820.2053.33pH 9.5, 37 °CPMID 17052977Open
NS2B-NS3 proteasevariant NS2B-Q86AWest Nile virusN-acetyl-L-leucyl-L-glutaminyl-L-tyrosyl-L-threonyl-L-lysyl-L-arginine para-nitroanilide0.1271.2030.105pH 9.5, 37 °CPMID 17052977Open
NS2B-NS3 proteasevariant NS2B-Q86AWest Nile virusN-acetyl-L-leucyl-L-lysyl-L-lysyl-L-arginine para-nitroanilide0.3670.2581.424pH 9.5, 37 °CPMID 17052977Open
NS2B-NS3 proteasevariant NS2B-Q86EWest Nile virusN-acetyl-L-leucyl-L-lysyl-L-lysyl-L-arginine para-nitroanilide0.8680.077711.18pH 9.5, 37 °CPMID 17052977Open
NS2B-NS3 proteasevariant NS2B-Q86LWest Nile virusN-acetyl-L-leucyl-L-lysyl-L-lysyl-L-arginine para-nitroanilide0.9730.1486.586pH 9.5, 37 °CPMID 17052977Open
NS2B-NS3 proteasevariant NS2B-V75AWest Nile virusN-acetyl-L-leucyl-L-glutaminyl-L-tyrosyl-L-threonyl-L-lysyl-L-arginine para-nitroanilide0.1645.3180.031pH 9.5, 37 °CPMID 17052977Open
NS2B-NS3 proteasevariant NS2B-V75AWest Nile virusN-acetyl-L-leucyl-L-lysyl-L-lysyl-L-arginine para-nitroanilide0.1730.1990.866pH 9.5, 37 °CPMID 17052977Open
NS2B-NS3 proteasevariant NS2B-V75FWest Nile virusN-acetyl-L-leucyl-L-glutaminyl-L-tyrosyl-L-threonyl-L-lysyl-L-arginine para-nitroanilide0.0910.8090.113pH 9.5, 37 °CPMID 17052977Open
NS2B-NS3 proteasevariant NS2B-V75FWest Nile virusN-acetyl-L-leucyl-L-lysyl-L-lysyl-L-arginine para-nitroanilide0.2460.2720.905pH 9.5, 37 °CPMID 17052977Open
NS2B-NS3 proteasevariant NS3-A164SWest Nile virusN-acetyl-L-leucyl-L-lysyl-L-lysyl-L-arginine para-nitroanilide0.260.2211.178pH 9.5, 37 °CPMID 17052977Open
NS2B-NS3 proteasevariant NS3-A164VWest Nile virusN-acetyl-L-leucyl-L-lysyl-L-lysyl-L-arginine para-nitroanilide0.1810.3310.546pH 9.5, 37 °CPMID 17052977Open
NS2B-NS3 proteasevariant NS3-D129AWest Nile virusN-acetyl-L-leucyl-L-lysyl-L-lysyl-L-arginine para-nitroanilide0.0480.6350.075pH 9.5, 37 °CPMID 17052977Open
NS2B-NS3 proteasevariant NS3-D129EWest Nile virusN-acetyl-L-leucyl-L-lysyl-L-lysyl-L-arginine para-nitroanilide0.0360.4320.083pH 9.5, 37 °CPMID 17052977Open
NS2B-NS3 proteasevariant NS3-I155FWest Nile virusN-acetyl-L-leucyl-L-lysyl-L-lysyl-L-arginine para-nitroanilide0.7330.2073.532pH 9.5, 37 °CPMID 17052977Open
NS2B-NS3 proteasevariant NS3-I162FWest Nile virusN-acetyl-L-leucyl-L-glutaminyl-L-tyrosyl-L-threonyl-L-lysyl-L-arginine para-nitroanilide0.040.4160.095pH 9.5, 37 °CPMID 17052977Open
NS2B-NS3 proteasevariant NS3-I162FWest Nile virusN-acetyl-L-leucyl-L-lysyl-L-lysyl-L-arginine para-nitroanilide0.1640.2680.612pH 9.5, 37 °CPMID 17052977Open
NS2B-NS3 proteasevariant NS3-M156AWest Nile virusN-acetyl-L-leucyl-L-glutaminyl-L-tyrosyl-L-threonyl-L-lysyl-L-arginine para-nitroanilide0.3140.8790.357pH 9.5, 37 °CPMID 17052977Open
NS2B-NS3 proteasevariant NS3-M156AWest Nile virusN-acetyl-L-leucyl-L-lysyl-L-lysyl-L-arginine para-nitroanilide0.7780.1644.741pH 9.5, 37 °CPMID 17052977Open
NS2B-NS3 proteasevariant NS3-T111FWest Nile virusN-acetyl-L-leucyl-L-glutaminyl-L-tyrosyl-L-threonyl-L-lysyl-L-arginine para-nitroanilide0.0510.3760.135pH 9.5, 37 °CPMID 17052977Open
NS2B-NS3 proteasevariant NS3-T111FWest Nile virusN-acetyl-L-leucyl-L-lysyl-L-lysyl-L-arginine para-nitroanilide0.1980.1691.176pH 9.5, 37 °CPMID 17052977Open
NS2B-NS3 proteasevariant NS3-T111LWest Nile virusN-acetyl-L-leucyl-L-glutaminyl-L-tyrosyl-L-threonyl-L-lysyl-L-arginine para-nitroanilide0.0180.6170.03pH 9.5, 37 °CPMID 17052977Open
NS2B-NS3 proteasevariant NS3-T111LWest Nile virusN-acetyl-L-leucyl-L-lysyl-L-lysyl-L-arginine para-nitroanilide0.060.2140.279pH 9.5, 37 °CPMID 17052977Open
NS2B-NS3 proteasevariant NS3-V154FWest Nile virus2-naphthoyl-L-lysyl-L-lysyl-L-arginine para-nitroanilide0.04310.09770.442pH 9.5, 37 °CPMID 17052977Open
NS2B-NS3 proteasevariant NS3-V154FWest Nile virusN-acetyl-L-leucyl-L-lysyl-L-lysyl-L-arginine para-nitroanilide0.0570.2630.216pH 9.5, 37 °CPMID 17052977Open
NS2B-NS3 proteasevariant NS3-V154LWest Nile virusN-acetyl-L-leucyl-L-glutaminyl-L-tyrosyl-L-threonyl-L-lysyl-L-arginine para-nitroanilide0.2450.3440.712pH 9.5, 37 °CPMID 17052977Open
NS2B-NS3 proteasevariant NS3-V154LWest Nile virusN-acetyl-L-leucyl-L-lysyl-L-lysyl-L-arginine para-nitroanilide0.80.194.223pH 9.5, 37 °CPMID 17052977Open
NS2B-NS3 proteasevariant NS3-V166LWest Nile virusN-acetyl-L-leucyl-L-glutaminyl-L-tyrosyl-L-threonyl-L-lysyl-L-arginine para-nitroanilide0.2970.2321.282pH 9.5, 37 °CPMID 17052977Open
NS2B-NS3 proteasevariant NS3-V166LWest Nile virusN-acetyl-L-leucyl-L-lysyl-L-lysyl-L-arginine para-nitroanilide0.6380.0966.676pH 9.5, 37 °CPMID 17052977Open
NS2B-NS3 proteasevariant R95*A/R29GZika virusAc-Arg-Lys-Lys-Arg-ACC1.080.00418258PMID 28034744Open
NS2B-NS3 proteasevariant R95*A/R29GZika virusAc-DArg-Lys-Orn-Arg(Me)-ACC5.430.006905PMID 28034744Open
NS2B-NS3 proteasevariant R95*A/R29GZika virusAc-DArg-Lys-Orn-Arg-ACC4.640.00421.1 × 10³PMID 28034744Open
NS2B-NS3 proteasevariant R95*A/R29GZika virusAc-DLys-Lys-Orn-Arg-ACC7.990.00799999PMID 28034744Open
NS2B-NS3 proteasevariant R95*A/R29GZika virusAc-Nle-Lys-Lys-Arg-ACC4.40.01385318PMID 28034744Open
NS2B/NS3 proteaseYellow fever virus2-aminobenzoyl-Ala-Arg-Arg-Arg-Ser-Gln-N-(2,4-dinitrophenyl)ethylenediamine0.120.009612.5pH 9, 37 °CPMID 21419753Open
NS2B/NS3 proteaseYellow fever virus2-aminobenzoyl-Ala-Arg-Arg-Lys-Ser-Gln-N-(2,4-dinitrophenyl)ethylenediamine0.0050.00153.33pH 9, 37 °CPMID 21419753Open
NS2B/NS3 proteaseYellow fever virus2-aminobenzoyl-Ala-Arg-Lys-Arg-Ser-Gln-N-(2,4-dinitrophenyl)ethylenediamine0.060.01474.08pH 9, 37 °CPMID 21419753Open
NS2B/NS3 proteaseYellow fever virus2-aminobenzoyl-Ala-Arg-Lys-Lys-Ser-Gln-N-(2,4-dinitrophenyl)ethylenediamine0.0021.3 × 10⁻⁴18.16pH 9, 37 °CPMID 21419753Open
NS2B/NS3 proteaseYellow fever virus2-aminobenzoyl-Ala-Gly-Arg-Arg-Ser-Ala-Gln-N-(2,4-dinitrophenyl)ethylenediamine0.0610.023.04pH 9, 37 °CPMID 21419753Open
NS2B/NS3 proteaseYellow fever virus2-aminobenzoyl-Ala-Gly-Arg-Lys-Ser-Ala-Gln-N-(2,4-dinitrophenyl)ethylenediamine0.0050.00511pH 9, 37 °CPMID 21419753Open
NS2B/NS3 proteaseYellow fever virus2-aminobenzoyl-Ala-Gly-Lys-Arg-Ser-Ala-Gln-N-(2,4-dinitrophenyl)ethylenediamine0.010.00731.37pH 9, 37 °CPMID 21419753Open
NS2B/NS3 proteaseYellow fever virus2-aminobenzoyl-Ala-Gly-Lys-Lys-Ser-Ala-Gln-N-(2,4-dinitrophenyl)ethylenediamine0.0010.00870.11pH 9, 37 °CPMID 21419753Open
NS2B/NS3 proteaseYellow fever virus2-aminobenzoyl-Ala-Lys-Arg-Arg-Ser-Gln-N-(2,4-dinitrophenyl)ethylenediamine0.0260.00213pH 9, 37 °CPMID 21419753Open
NS2B/NS3 proteaseYellow fever virus2-aminobenzoyl-Ala-Lys-Arg-Lys-Ser-Gln-N-(2,4-dinitrophenyl)ethylenediamine0.0126 × 10⁻⁴20pH 9, 37 °CPMID 21419753Open
NS2B/NS3 proteaseYellow fever virus2-aminobenzoyl-Ala-Lys-Lys-Arg-Ser-Gln-N-(2,4-dinitrophenyl)ethylenediamine0.0970.007612.8pH 9, 37 °CPMID 21419753Open
NS2B/NS3 proteaseYellow fever virus2-aminobenzoyl-Ala-Lys-Lys-Lys-Ser-Gln-N-(2,4-dinitrophenyl)ethylenediamine0.0040.00660.6pH 9, 37 °CPMID 21419753Open
NS2B/NS3 proteaseYellow fever virus2-aminobenzoyl-Arg-Ile-Phe-Gly-Arg-Arg-Ser-Ile-Pro-Val-Asn-Glu-Gln-N-(2,4-dinitrophenyl)ethylenediamine0.0050.020.25pH 9, 37 °CPMID 21419753Open
NS2B/NS3 proteaseYellow fever virus2-aminobenzoyl-Lys-Arg-Arg-Ser-Ser-Lys-Gln-N-(2,4-dinitrophenyl)ethylenediamine0.0250.002111.9pH 9, 37 °CPMID 21419753Open
NS2B/NS3 proteaseYellow fever virus2-aminobenzoyl-Lys-Leu-Ala-Gln-Arg-Arg-Val-Phe-His-Gly-Val-Ala-Gln-N-(2,4-dinitrophenyl)ethylenediamine0.0030.00271.1pH 9, 37 °CPMID 21419753Open
NS2B/NS3 proteaseYellow fever virus2-aminobenzoyl-Lys-Lys-Arg-Ser-Ser-Lys-Gln-N-(2,4-dinitrophenyl)ethylenediamine0.0120.0121pH 9, 37 °CPMID 21419753Open
NS2B/NS3 proteaseYellow fever virus2-aminobenzoyl-Met-Lys-Thr-Gly-Arg-Arg-Gly-Ser-Ala-Asn-Gly-Lys-Gln-N-(2,4-dinitrophenyl)ethylenediamine0.0120.00254.8pH 9, 37 °CPMID 21419753Open
NS2B/NS3 proteaseYellow fever virus2-aminobenzoyl-Phe-Ala-Glu-Gly-Arg-Arg-Gly-Ala-Ala-Glu-Val-Leu-Gln-N-(2,4-dinitrophenyl)ethylenediamine0.0010.0150.07pH 9, 37 °CPMID 21419753Open
NS2B/NS3 proteaseYellow fever virus2-aminobenzoyl-Ser-Ala-Ala-Gln-Arg-Arg-Gly-Arg-Ile-Gly-Arg-Asn-Gln-N-(2,4-dinitrophenyl)ethylenediamine0.0160.00881.8pH 9, 37 °CPMID 21419753Open
NS2B/NS3 proteaseYellow fever virus2-aminobenzoyl-Ser-Ser-Arg-Lys-Arg-Arg-Ser-His-Asp-Val-Leu-Thr-Gln-N-(2,4-dinitrophenyl)ethylenediamine0.0690.002231.4pH 9, 37 °CPMID 21419753Open
NS2B/NS3 proteaseYellow fever virus2-aminobenzoyl-Val-Arg-Gly-Ala-Arg-Arg-Ser-Gly-Asp-Val-Leu-Trp-Gln-N-(2,4-dinitrophenyl)ethylenediamine0.0410.00854.8pH 9, 37 °CPMID 21419753Open
ΔNS2B-NS3pro (NS2B/NS3 protease complex)Bussuquara virust-butyloxycarbonyl-glycyl-L-arginyl-L-arginine 4-methylcoumaryl-7-amide0.0732.180.033pH 9, 37 °CPMID 16504332Open
ΔNS2B-NS3pro (NS2B/NS3 protease complex)Bussuquara virust-butyloxycarbonyl-glycyl-L-lysyl-L-arginine 4-methylcoumaryl-7-amide0.0391.2490.031pH 9, 37 °CPMID 16504332Open
ΔNS2B-NS3pro (NS2B/NS3 protease complex)Dengue virus type 3t-butyloxycarbonyl-glycyl-L-arginyl-L-arginine 4-methylcoumaryl-7-amide0.1632.70.06pH 9, 37 °CPMID 16504332Open
ΔNS2B-NS3pro (NS2B/NS3 protease complex)Dengue virus type 3t-butyloxycarbonyl-glycyl-L-lysyl-L-arginine 4-methylcoumaryl-7-amide0.0070.1810.034pH 9, 37 °CPMID 16504332Open
ΔNS2B-NS3pro (NS2B/NS3 protease complex)Langat virust-butyloxycarbonyl-glycyl-L-arginyl-L-arginine 4-methylcoumaryl-7-amide0.0070.3240.02pH 9, 37 °CPMID 16504332Open
ΔNS2B-NS3pro (NS2B/NS3 protease complex)St. Louis encephalitis virust-butyloxycarbonyl-glycyl-L-arginyl-L-arginine 4-methylcoumaryl-7-amide0.0750.4650.161pH 9, 37 °CPMID 16504332Open
ΔNS2B-NS3pro (NS2B/NS3 protease complex)St. Louis encephalitis virust-butyloxycarbonyl-glycyl-L-lysyl-L-arginine 4-methylcoumaryl-7-amide0.0830.5630.149pH 9, 37 °CPMID 16504332Open
ΔNS2B-NS3pro (NS2B/NS3 protease complex)Yellow fever virust-butyloxycarbonyl-glycyl-L-arginyl-L-arginine 4-methylcoumaryl-7-amide0.0440.4150.105pH 9, 37 °CPMID 16504332Open
ΔNS2B-NS3pro (NS2B/NS3 protease complex)Yellow fever virust-butyloxycarbonyl-glycyl-L-lysyl-L-arginine 4-methylcoumaryl-7-amide0.0060.530.011pH 9, 37 °CPMID 16504332Open
ΔNS2B-NS3pro (NS2B/NS3 protease complex)Zika virust-butyloxycarbonyl-glycyl-L-arginyl-L-arginine 4-methylcoumaryl-7-amide0.0130.4170.031pH 9, 37 °CPMID 16504332Open
ΔNS2B-NS3pro (NS2B/NS3 protease complex)Zika virust-butyloxycarbonyl-glycyl-L-lysyl-L-arginine 4-methylcoumaryl-7-amide0.0880.650.136pH 9, 37 °CPMID 16504332Open