CatLogenzyme kinetics catalog

Values CatLog read from this paper

Substrate specificity of recombinant dengue 2 virus NS2B-NS3 protease: influence of natural and unnatural basic amino acids on hydrolysis of synthetic fluorescent substrates

PMID 17184724doi:10.1016/j.abb.2006.11.005Open in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 51 records (135 values) from this paper: dengue 2 virus NS2B-NS3 protease (CF40glyNS3pro), human furin (hfurin) hfurin, recombinant human furin, dengue 2 virus NS2B-NS3 protease (CF40glyNS3pro), strain NGC and 1 more from dengue 2 virus, Homo sapiens, Dengue virus 2.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.

EC 3.4.21.91

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)Conditions
dengue 2 virus NS2B-NS3 protease (CF40glyNS3pro)variant strain BR/01-EN (NS3_{BR/01})dengue 2 virusAbz–EVKKQRAGVLWDQ–EDDnp (peptide 2)——1.6pH 9, 37 °COpen
dengue 2 virus NS2B-NS3 protease (CF40glyNS3pro)variant strain BR/01-EN (NS3_{BR/01})dengue 2 virusAbz–HRREKRSVALQ–EDDnp (prM/M* site peptide)——0.8pH 9, 37 °COpen
dengue 2 virus NS2B-NS3 protease (CF40glyNS3pro)variant strain BR/01-EN (NS3_{BR/01})dengue 2 virusAbz–LNRRRRTAGMIIQ–EDDnp (peptide 5)——5.1pH 9, 37 °COpen
dengue 2 virus NS2B-NS3 protease (CF40glyNS3pro)variant strain BR/01-EN (NS3_{BR/01})dengue 2 virusAbz–RTSKKRSWPLNEQ–EDDnp (peptide 1)——2.9pH 9, 37 °COpen
dengue 2 virus NS2B-NS3 protease (CF40glyNS3pro)variant strain BR/01-EN (NS3_{BR/01})dengue 2 virusAbz–SAAQRRGRIGRNQ–EDDnp (peptide 6)——15.8pH 9, 37 °COpen
dengue 2 virus NS2B-NS3 protease (CF40glyNS3pro)variant strain BR/01-EN (NS3_{BR/01})dengue 2 virusAbz–TTSTRRGTGNIGQ–EDDnp (peptide 4)——0.7pH 9, 37 °COpen
dengue 2 virus NS2B-NS3 protease (CF40glyNS3pro)variant strain NGC (NS3_{NGC})dengue 2 virusAbz–AGKKSAQ–EDDnp (peptide 18)0.020.05880.3pH 9, 37 °COpen
dengue 2 virus NS2B-NS3 protease (CF40glyNS3pro)variant strain NGC (NS3_{NGC})dengue 2 virusAbz–AGKRSAQ–EDDnp (peptide 20)0.20.0385.3pH 9, 37 °COpen
dengue 2 virus NS2B-NS3 protease (CF40glyNS3pro)variant strain NGC (NS3_{NGC})dengue 2 virusAbz–AGRKSAQ–EDDnp (peptide 19)0.130.02136.1pH 9, 37 °COpen
dengue 2 virus NS2B-NS3 protease (CF40glyNS3pro)variant strain NGC (NS3_{NGC})dengue 2 virusAbz–AGRRSAQ–EDDnp (peptide 17)0.210.0210.5pH 9, 37 °COpen
dengue 2 virus NS2B-NS3 protease (CF40glyNS3pro)variant strain NGC (NS3_{NGC})dengue 2 virusAbz–AKKKSQ–EDDnp (peptide 26)0.040.06920.6pH 9, 37 °COpen
dengue 2 virus NS2B-NS3 protease (CF40glyNS3pro)variant strain NGC (NS3_{NGC})dengue 2 virusAbz–AKKRSQ–EDDnp (peptide 28)0.070.05321.3pH 9, 37 °COpen
dengue 2 virus NS2B-NS3 protease (CF40glyNS3pro)variant strain NGC (NS3_{NGC})dengue 2 virusAbz–AKRKSQ–EDDnp (peptide 27)0.040.02371.7pH 9, 37 °COpen
dengue 2 virus NS2B-NS3 protease (CF40glyNS3pro)variant strain NGC (NS3_{NGC})dengue 2 virusAbz–AKRRSQ–EDDnp (peptide 22)10.031431.8pH 9, 37 °COpen
dengue 2 virus NS2B-NS3 protease (CF40glyNS3pro)variant strain NGC (NS3_{NGC})dengue 2 virusAbz–ARKKSQ–EDDnp (peptide 25)0.050.06730.7pH 9, 37 °COpen
dengue 2 virus NS2B-NS3 protease (CF40glyNS3pro)variant strain NGC (NS3_{NGC})dengue 2 virusAbz–ARKRSQ–EDDnp (peptide 23)0.070.0581.2pH 9, 37 °COpen
dengue 2 virus NS2B-NS3 protease (CF40glyNS3pro)variant strain NGC (NS3_{NGC})dengue 2 virusAbz–ARRKSQ–EDDnp (peptide 24)0.090.01834.9pH 9, 37 °COpen
dengue 2 virus NS2B-NS3 protease (CF40glyNS3pro)variant strain NGC (NS3_{NGC})dengue 2 virusAbz–ARRRSQ–EDDnp (peptide 21)0.380.025614.8pH 9, 37 °COpen
dengue 2 virus NS2B-NS3 protease (CF40glyNS3pro)variant strain NGC (NS3_{NGC})dengue 2 virusAbz–EVKKQRAGVLWDQ–EDDnp (peptide 2)0.030.00883.4pH 9, 37 °COpen
dengue 2 virus NS2B-NS3 protease (CF40glyNS3pro)variant strain NGC (NS3_{NGC})dengue 2 virusAbz–FAAGRKSLTLNLQ–EDDnp (peptide 3)0.020.01771.2pH 9, 37 °COpen
dengue 2 virus NS2B-NS3 protease (CF40glyNS3pro)variant strain NGC (NS3_{NGC})dengue 2 virusAbz–FAAGRRSLTLNLQ–EDDnp0.620.01255pH 9, 37 °COpen
dengue 2 virus NS2B-NS3 protease (CF40glyNS3pro)variant strain NGC (NS3_{NGC})dengue 2 virusAbz–FAAQRRGRIGRNQ–EDDnp (peptide 8)0.140.007419.4pH 9, 37 °COpen
dengue 2 virus NS2B-NS3 protease (CF40glyNS3pro)variant strain NGC (NS3_{NGC})dengue 2 virusAbz–HRREKRSVALQ–EDDnp (prM/M* site peptide)0.040.0261.5pH 9, 37 °COpen
dengue 2 virus NS2B-NS3 protease (CF40glyNS3pro)variant strain NGC (NS3_{NGC})dengue 2 virusAbz–LNRRRRTAGMIIQ–EDDnp (peptide 5)0.040.003910.2pH 9, 37 °COpen
dengue 2 virus NS2B-NS3 protease (CF40glyNS3pro)variant strain NGC (NS3_{NGC})dengue 2 virusAbz–RTSKKRSWPLNEQ–EDDnp (peptide 1)FRET assay; fluorescence 420 nm with excitation at 320 nm0.170.035.7pH 9, 37 °COpen
dengue 2 virus NS2B-NS3 protease (CF40glyNS3pro)variant strain NGC (NS3_{NGC})dengue 2 virusAbz–RTSKKRSWPLNEQ–EDDnp (peptide 1)37 °C; reaction stopped with 0.33% trifluoroacetic acid—0.0391—pH 9, 37 °COpen
dengue 2 virus NS2B-NS3 protease (CF40glyNS3pro)variant strain NGC (NS3_{NGC})dengue 2 virusAbz–SAAGRRGRIGRNQ–EDDnp (peptide 9)0.090.00422pH 9, 37 °COpen
dengue 2 virus NS2B-NS3 protease (CF40glyNS3pro)variant strain NGC (NS3_{NGC})dengue 2 virusAbz–SAAQRKGRIGRNQ–EDDnp (peptide 10)0.040.00984.6pH 9, 37 °COpen
dengue 2 virus NS2B-NS3 protease (CF40glyNS3pro)variant strain NGC (NS3_{NGC})dengue 2 virusAbz–SAAQRRGLIGRNQ–EDDnp (peptide 12)0.110.007215.5pH 9, 37 °COpen
dengue 2 virus NS2B-NS3 protease (CF40glyNS3pro)variant strain NGC (NS3_{NGC})dengue 2 virusAbz–SAAQRRGRIGNNQ–EDDnp (peptide 15)0.110.004623.1pH 9, 37 °COpen
dengue 2 virus NS2B-NS3 protease (CF40glyNS3pro)variant strain NGC (NS3_{NGC})dengue 2 virusAbz–SAAQRRGRIGRLQ–EDDnp (peptide 16)0.160.004535.9pH 9, 37 °COpen
dengue 2 virus NS2B-NS3 protease (CF40glyNS3pro)variant strain NGC (NS3_{NGC})dengue 2 virusAbz–SAAQRRGRIGRNQ–EDDnp (peptide 6)0.290.013821pH 9, 37 °COpen
dengue 2 virus NS2B-NS3 protease (CF40glyNS3pro)variant strain NGC (NS3_{NGC})dengue 2 virusAbz–SAAQRRGRILRNQ–EDDnp (peptide 14)0.180.006926.5pH 9, 37 °COpen
dengue 2 virus NS2B-NS3 protease (CF40glyNS3pro)variant strain NGC (NS3_{NGC})dengue 2 virusAbz–SAAQRRGRTGRNQ–EDDnp (peptide 13)0.340.016520.9pH 9, 37 °COpen
dengue 2 virus NS2B-NS3 protease (CF40glyNS3pro)variant strain NGC (NS3_{NGC})dengue 2 virusAbz–SAAQRRSRIGRNQ–EDDnp (peptide 11)0.140.005923.5pH 9, 37 °COpen
dengue 2 virus NS2B-NS3 protease (CF40glyNS3pro)variant strain NGC (NS3_{NGC})dengue 2 virusAbz–TTSTRRGTGNIGQ–EDDnp (peptide 4)0.040.0271.5pH 9, 37 °COpen
dengue 2 virus NS2B-NS3 protease (CF40glyNS3pro)variant strain NGC (NS3_{NGC})dengue 2 virusBz–(3-pyridyl-alanine)–Arg–MCA, Bz = benzoyl group0.0020.0430.05pH 9, 37 °COpen
dengue 2 virus NS2B-NS3 protease (CF40glyNS3pro)variant strain NGC (NS3_{NGC})dengue 2 virusBz–(4-aminocyclohexyl-alanine)–Arg–MCA, Bz = benzoyl group0.0130.0270.48pH 9, 37 °COpen
dengue 2 virus NS2B-NS3 protease (CF40glyNS3pro)variant strain NGC (NS3_{NGC})dengue 2 virusBz–(4-aminomethyl-cyclohexyl-alanine)–Arg–MCA, Bz = benzoyl group0.1720.2980.58pH 9, 37 °COpen
dengue 2 virus NS2B-NS3 protease (CF40glyNS3pro)variant strain NGC (NS3_{NGC})dengue 2 virusBz–(4-aminomethyl-N-isopropyl-phenylalanine)–Arg–MCA, Bz = benzoyl group0.0220.3540.06pH 9, 37 °COpen
dengue 2 virus NS2B-NS3 protease (CF40glyNS3pro)variant strain NGC (NS3_{NGC})dengue 2 virusBz–(4-aminomethyl-phenylalanine)–Arg–MCA, Bz = benzoyl group0.0130.0260.5pH 9, 37 °COpen
dengue 2 virus NS2B-NS3 protease (CF40glyNS3pro)variant strain NGC (NS3_{NGC})dengue 2 virusBz–(4-guanidine phenylalanine)–Arg–MCA, Bz = benzoyl group0.0350.490.07pH 9, 37 °COpen
dengue 2 virus NS2B-NS3 protease (CF40glyNS3pro)variant strain NGC (NS3_{NGC})dengue 2 virusBz–(4-piperidinyl-alanine)–Arg–MCA, Bz = benzoyl group0.0140.2160.07pH 9, 37 °COpen
dengue 2 virus NS2B-NS3 protease (CF40glyNS3pro)variant strain NGC (NS3_{NGC})dengue 2 virusBz–Arg–Arg–MCA (peptide 31)0.1090.2470.44pH 9, 37 °COpen
dengue 2 virus NS2B-NS3 protease (CF40glyNS3pro), strain BR/01-ENvariant CF40glyNS3pro, dengue 2 virus strain BR/01-ENDengue virus 2Abz–FAAGRKSLTLNLQ–EDDnp——0.6pH 9, 37 °COpen
dengue 2 virus NS2B-NS3 protease (CF40glyNS3pro), strain NGCvariant CF40glyNS3pro, dengue 2 virus strain NGCDengue virus 2Abz–RTSKKRSWPLNEQ–EDDnp—0.03—pH 9, 37 °COpen
dengue 2 virus NS2B-NS3 protease (CF40glyNS3pro), strain NGCvariant CF40glyNS3pro, dengue 2 virus strain NGCDengue virus 2Z–RR–MCA0.110.2470.5pH 9, 37 °COpen
human furin (hfurin) hfurin, recombinant human furinHomo sapiensAbz–HRREKRSVALQ–EDDnp0.811.8 × 10⁻⁴4.5 × 10³pH 7, 37 °COpen
human furin (hfurin) hfurin, recombinant human furinHomo sapiensAbz–LNRRRRTAGMIIQ–EDDnp0.233.3 × 10⁻⁴697pH 7, 37 °COpen
human furin (hfurin) hfurin, recombinant human furinHomo sapiensAbz–RTSKKRSWPLNEQ–EDDnp0.024.4 × 10⁻⁴38pH 7, 37 °COpen
human furin (hfurin) hfurin, recombinant human furinHomo sapiensAbz–SAAQRRGRIGRNQ–EDDnp0.170.00132129pH 7, 37 °COpen