EC 3.4.22.28: picornain 3C
HydrolasesOpen in Browse
CatLog read 38 records for EC 3.4.22.28 from 3 papers, for 3 organisms. Names in these papers: HRV 3C protease, poliovirus picornain 3C protease (PV3C), EV71 3C protease.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units.
CatLog also holds 26 records for EC 3.4.22.28 that are not yet re-read: from its earlier version, built from the enzyme databases and partly checked against the papers. All records for EC 3.4.22.28 in Browse.
Papers
| Paper | Organisms | Records |
|---|---|---|
| Kosmotropic salt activation and substrate specificity of poliovirus protease 3C | poliovirus | 14 |
| A combinatorial strategy for HRV 3C protease engineering to achieve the N-terminal free cleavage | Human rhinovirus | 14 |
| Biochemical characterization of recombinant Enterovirus 71 3C protease with fluorogenic model peptide substrates and development of a biochemical assay | Enterovirus 71 | 10 |
Records
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Paper | Open record |
|---|---|---|---|---|---|---|---|---|
| EV71 3C protease | Enterovirus 71 | Dabcyl-IEALFQGPPKFRE-Edans | 0.0028 | 0.0632 | 0.0448 | pH 7, 30 °C | PMID 25421478 | Open record: EV71 3C protease, Dabcyl-IEALFQGPPKFRE-Edans |
| EV71 3C protease | Enterovirus 71 | Dabcyl-RTATVQGPSLDFKE-Edans | 0.002 | 0.0825 | 0.0242 | pH 7, 30 °C | PMID 25421478 | Open record: EV71 3C protease, Dabcyl-RTATVQGPSLDFKE-Edans |
| EV71 3C protease | Enterovirus 71 | NMA-DEAMEQGVSDYIK-DNP | 5 × 10⁻⁴ | 0.0735 | 0.0067 | pH 7, 30 °C | PMID 25421478 | Open record: EV71 3C protease, NMA-DEAMEQGVSDYIK-DNP |
| EV71 3C protease | Enterovirus 71 | NMA-IAALFQGPPK(DNP)FR | 0.0027 | 0.075 | 0.0355 | pH 7, 30 °C | PMID 25421478 | Open record: EV71 3C protease, NMA-IAALFQGPPK(DNP)FR |
| EV71 3C protease | Enterovirus 71 | NMA-IEAAFQGPPK(DNP)FR | 0.005 | 0.0667 | 0.075 | pH 7, 30 °C | PMID 25421478 | Open record: EV71 3C protease, NMA-IEAAFQGPPK(DNP)FR |
| EV71 3C protease | Enterovirus 71 | NMA-IEALFQGPPK(DNP)FR | 0.0085 | 0.0431 | 0.197 | pH 7, 30 °C | PMID 25421478 | Open record: EV71 3C protease, NMA-IEALFQGPPK(DNP)FR |
| EV71 3C protease | Enterovirus 71 | NMA-IELLFQGPPK(DNP)FR | 0.004 | 0.0482 | 0.083 | pH 7, 30 °C | PMID 25421478 | Open record: EV71 3C protease, NMA-IELLFQGPPK(DNP)FR |
| EV71 3C protease | Enterovirus 71 | NMA-RQAVTQGFPTELK-DNP | 0.0033 | 0.0924 | 0.037 | pH 7, 30 °C | PMID 25421478 | Open record: EV71 3C protease, NMA-RQAVTQGFPTELK-DNP |
| EV71 3C protease | Enterovirus 71 | NMA-RTATVQGPSLDFK(DNP) | 0.0035 | 0.0582 | 0.06 | pH 7, 30 °C | PMID 25421478 | Open record: EV71 3C protease, NMA-RTATVQGPSLDFK(DNP) |
| EV71 3C protease | Enterovirus 71 | NMA-TSAVLQSGFRK(DNP)M | 3.3 × 10⁻⁴ | 0.075 | 0.0045 | pH 7, 30 °C | PMID 25421478 | Open record: EV71 3C protease, NMA-TSAVLQSGFRK(DNP)M |
| HRV 3C protease | Human rhinovirus | MBP-LEVLFQ↓A-6His-GFP fusion protein (P1' residue Ala) | 0.032 | 0.034 | 0.91 | pH 8, 4 °C | PMID 38521339 | Open record: HRV 3C protease, MBP-LEVLFQ↓A-6His-GFP fusion protein (P1' residue Ala) |
| HRV 3C protease | Human rhinovirus | MBP-LEVLFQ↓C-6His-GFP fusion protein (P1' residue Cys) | 0.021 | 0.045 | 0.47 | pH 8, 4 °C | PMID 38521339 | Open record: HRV 3C protease, MBP-LEVLFQ↓C-6His-GFP fusion protein (P1' residue Cys) |
| HRV 3C protease | Human rhinovirus | MBP-LEVLFQ↓G-6His-GFP fusion protein (P1' residue Gly) | 0.22 | 0.047 | 4.73 | pH 8, 4 °C | PMID 38521339 | Open record: HRV 3C protease, MBP-LEVLFQ↓G-6His-GFP fusion protein (P1' residue Gly) |
| HRV 3C protease | Human rhinovirus | MBP-LEVLFQ↓S-6His-GFP fusion protein (P1' residue Ser) | 0.021 | 0.049 | 0.39 | pH 8, 4 °C | PMID 38521339 | Open record: HRV 3C protease, MBP-LEVLFQ↓S-6His-GFP fusion protein (P1' residue Ser) |
| HRV 3C proteasevariant K22L/F25V/N106Y/T143M/Q145M/T129I | Human rhinovirus | MBP-LEVLFQ↓A-6His-GFP fusion protein (P1' residue Ala) | 0.077 | 0.027 | 2.84 | pH 8, 4 °C | PMID 38521339 | Open record: HRV 3C protease variant K22L/F25V/N106Y/T143M/Q145M/T129I, MBP-LEVLFQ↓A-6His-GFP fusion protein (P1' residue Ala) |
| HRV 3C proteasevariant K22L/F25V/N106Y/T143M/Q145M/T129I | Human rhinovirus | MBP-LEVLFQ↓C-6His-GFP fusion protein (P1' residue Cys) | 0.14 | 0.053 | 2.65 | pH 8, 4 °C | PMID 38521339 | Open record: HRV 3C protease variant K22L/F25V/N106Y/T143M/Q145M/T129I, MBP-LEVLFQ↓C-6His-GFP fusion protein (P1' residue Cys) |
| HRV 3C proteasevariant K22L/F25V/N106Y/T143M/Q145M/T129I | Human rhinovirus | MBP-LEVLFQ↓G-6His-GFP fusion protein (P1' residue Gly) | 0.16 | 0.045 | 3.62 | pH 8, 4 °C | PMID 38521339 | Open record: HRV 3C protease variant K22L/F25V/N106Y/T143M/Q145M/T129I, MBP-LEVLFQ↓G-6His-GFP fusion protein (P1' residue Gly) |
| HRV 3C proteasevariant K22L/F25V/N106Y/T143M/Q145M/T129I | Human rhinovirus | MBP-LEVLFQ↓I-6His-GFP fusion protein (P1' residue Ile) | 0.063 | 0.038 | 1.71 | pH 8, 4 °C | PMID 38521339 | Open record: HRV 3C protease variant K22L/F25V/N106Y/T143M/Q145M/T129I, MBP-LEVLFQ↓I-6His-GFP fusion protein (P1' residue Ile) |
| HRV 3C proteasevariant K22L/F25V/N106Y/T143M/Q145M/T129I | Human rhinovirus | MBP-LEVLFQ↓L-6His-GFP fusion protein (P1' residue Leu) | 0.12 | 0.046 | 2.65 | pH 8, 4 °C | PMID 38521339 | Open record: HRV 3C protease variant K22L/F25V/N106Y/T143M/Q145M/T129I, MBP-LEVLFQ↓L-6His-GFP fusion protein (P1' residue Leu) |
| HRV 3C proteasevariant K22L/F25V/N106Y/T143M/Q145M/T129I | Human rhinovirus | MBP-LEVLFQ↓M-6His-GFP fusion protein (P1' residue Met) | 0.18 | 0.048 | 3.72 | pH 8, 4 °C | PMID 38521339 | Open record: HRV 3C protease variant K22L/F25V/N106Y/T143M/Q145M/T129I, MBP-LEVLFQ↓M-6His-GFP fusion protein (P1' residue Met) |
| HRV 3C proteasevariant K22L/F25V/N106Y/T143M/Q145M/T129I | Human rhinovirus | MBP-LEVLFQ↓N-6His-GFP fusion protein (P1' residue Asn) | 0.084 | 0.08 | 1.05 | pH 8, 4 °C | PMID 38521339 | Open record: HRV 3C protease variant K22L/F25V/N106Y/T143M/Q145M/T129I, MBP-LEVLFQ↓N-6His-GFP fusion protein (P1' residue Asn) |
| HRV 3C proteasevariant K22L/F25V/N106Y/T143M/Q145M/T129I | Human rhinovirus | MBP-LEVLFQ↓Q-6His-GFP fusion protein (P1' residue Gln) | 0.033 | 0.089 | 0.37 | pH 8, 4 °C | PMID 38521339 | Open record: HRV 3C protease variant K22L/F25V/N106Y/T143M/Q145M/T129I, MBP-LEVLFQ↓Q-6His-GFP fusion protein (P1' residue Gln) |
| HRV 3C proteasevariant K22L/F25V/N106Y/T143M/Q145M/T129I | Human rhinovirus | MBP-LEVLFQ↓S-6His-GFP fusion protein (P1' residue Ser) | 0.056 | 0.045 | 1.45 | pH 8, 4 °C | PMID 38521339 | Open record: HRV 3C protease variant K22L/F25V/N106Y/T143M/Q145M/T129I, MBP-LEVLFQ↓S-6His-GFP fusion protein (P1' residue Ser) |
| HRV 3C proteasevariant K22L/F25V/N106Y/T143M/Q145M/T129I | Human rhinovirus | MBP-LEVLFQ↓T-6His-GFP fusion protein (P1' residue Thr) | 0.08 | 0.065 | 1.23 | pH 8, 4 °C | PMID 38521339 | Open record: HRV 3C protease variant K22L/F25V/N106Y/T143M/Q145M/T129I, MBP-LEVLFQ↓T-6His-GFP fusion protein (P1' residue Thr) |
| poliovirus picornain 3C protease (PV3C)variant M27G | poliovirus | Abz-AALFQGPLQ-EDDnpno added salt | 0.01 | 0.0182 | 0.5 | pH 8, 25 °C | PMID 17002308 | Open record: poliovirus picornain 3C protease (PV3C) variant M27G, Abz-AALFQGPLQ-EDDnp |
| poliovirus picornain 3C protease (PV3C)variant M27G | poliovirus | Abz-AALFQGPLQ-EDDnp1 M sodium citrate | 0.96 | 0.02 | 48 | pH 8, 25 °C | PMID 17002308 | Open record: poliovirus picornain 3C protease (PV3C) variant M27G, Abz-AALFQGPLQ-EDDnp |
| poliovirus picornain 3C protease (PV3C)variant M27G | poliovirus | Abz-EAAFQGPLQ-EDDnpno added salt | 0.08 | 0.0349 | 2.2 | pH 8, 25 °C | PMID 17002308 | Open record: poliovirus picornain 3C protease (PV3C) variant M27G, Abz-EAAFQGPLQ-EDDnp |
| poliovirus picornain 3C protease (PV3C)variant M27G | poliovirus | Abz-EAAFQGPLQ-EDDnp1 M sodium citrate | 3.34 | 0.0264 | 126.6 | pH 8, 25 °C | PMID 17002308 | Open record: poliovirus picornain 3C protease (PV3C) variant M27G, Abz-EAAFQGPLQ-EDDnp |
| poliovirus picornain 3C protease (PV3C)variant M27G | poliovirus | Abz-EALAQGPLQ-EDDnpno added salt | 0.01 | 0.0413 | 0.3 | pH 8, 25 °C | PMID 17002308 | Open record: poliovirus picornain 3C protease (PV3C) variant M27G, Abz-EALAQGPLQ-EDDnp |
| poliovirus picornain 3C protease (PV3C)variant M27G | poliovirus | Abz-EALAQGPLQ-EDDnp1 M sodium citrate | 0.66 | 0.0435 | 15.1 | pH 8, 25 °C | PMID 17002308 | Open record: poliovirus picornain 3C protease (PV3C) variant M27G, Abz-EALAQGPLQ-EDDnp |
| poliovirus picornain 3C protease (PV3C)variant M27G | poliovirus | Abz-EALFQAPLQ-EDDnp | 0.34 | 0.0393 | 8.7 | pH 8, 25 °C | PMID 17002308 | Open record: poliovirus picornain 3C protease (PV3C) variant M27G, Abz-EALFQAPLQ-EDDnp |
| poliovirus picornain 3C protease (PV3C)variant M27G | poliovirus | Abz-EALFQGALQ-EDDnp | 0.28 | 0.0533 | 4.5 | pH 8, 25 °C | PMID 17002308 | Open record: poliovirus picornain 3C protease (PV3C) variant M27G, Abz-EALFQGALQ-EDDnp |
| poliovirus picornain 3C protease (PV3C)variant M27G | poliovirus | Abz-EALFQGPAQ-EDDnpno added salt | 0.03 | 0.0185 | 1.8 | pH 8, 25 °C | PMID 17002308 | Open record: poliovirus picornain 3C protease (PV3C) variant M27G, Abz-EALFQGPAQ-EDDnp |
| poliovirus picornain 3C protease (PV3C)variant M27G | poliovirus | Abz-EALFQGPAQ-EDDnp1 M sodium citrate | 2.72 | 0.0213 | 127.8 | pH 8, 25 °C | PMID 17002308 | Open record: poliovirus picornain 3C protease (PV3C) variant M27G, Abz-EALFQGPAQ-EDDnp |
| poliovirus picornain 3C protease (PV3C)variant M27G | poliovirus | Abz-EALFQGPLQ-EDDnpno added salt | 0.03 | 0.014 | 2.2 | pH 8, 25 °C | PMID 17002308 | Open record: poliovirus picornain 3C protease (PV3C) variant M27G, Abz-EALFQGPLQ-EDDnp |
| poliovirus picornain 3C protease (PV3C)variant M27G | poliovirus | Abz-EALFQGPLQ-EDDnp1 M sodium citrate | 0.87 | 0.016 | 54 | pH 8, 25 °C | PMID 17002308 | Open record: poliovirus picornain 3C protease (PV3C) variant M27G, Abz-EALFQGPLQ-EDDnp |
| poliovirus picornain 3C protease (PV3C)variant M27G | poliovirus | Abz-EALFQGPLQ-EDDnpno added salt | 0.06 | 0.0179 | 3.4 | 25 °C | PMID 17002308 | Open record: poliovirus picornain 3C protease (PV3C) variant M27G, Abz-EALFQGPLQ-EDDnp |
| poliovirus picornain 3C protease (PV3C)variant M27G | poliovirus | Abz-EALFQGPLQ-EDDnp1 M sodium citrate | 2.02 | 0.0203 | 99.5 | 25 °C | PMID 17002308 | Open record: poliovirus picornain 3C protease (PV3C) variant M27G, Abz-EALFQGPLQ-EDDnp |