Values CatLog read from this paper

A combinatorial strategy for HRV 3C protease engineering to achieve the N-terminal free cleavage

PMID 38521339doi:10.1016/j.ijbiomac.2024.131066Open in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 14 records (42 values) from this paper: HRV 3C protease from Human rhinovirus.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.

EC 2.7.7.48, EC 3.4.22.28, EC 3.4.22.29, EC 3.6.1.15

Records read from this paper
Enzyme	Organism	Substrate	k_cat (s⁻¹)	K_m (mM)	k_cat/K_m (mM⁻¹ s⁻¹)	Conditions	Open record
HRV 3C protease	Human rhinovirus	MBP-LEVLFQ↓A-6His-GFP fusion protein (P1' residue Ala)	0.032	0.034	0.91	pH 8, 4 °C	Open record: HRV 3C protease, MBP-LEVLFQ↓A-6His-GFP fusion protein (P1' residue Ala)
HRV 3C protease	Human rhinovirus	MBP-LEVLFQ↓C-6His-GFP fusion protein (P1' residue Cys)	0.021	0.045	0.47	pH 8, 4 °C	Open record: HRV 3C protease, MBP-LEVLFQ↓C-6His-GFP fusion protein (P1' residue Cys)
HRV 3C protease	Human rhinovirus	MBP-LEVLFQ↓G-6His-GFP fusion protein (P1' residue Gly)	0.22	0.047	4.73	pH 8, 4 °C	Open record: HRV 3C protease, MBP-LEVLFQ↓G-6His-GFP fusion protein (P1' residue Gly)
HRV 3C protease	Human rhinovirus	MBP-LEVLFQ↓S-6His-GFP fusion protein (P1' residue Ser)	0.021	0.049	0.39	pH 8, 4 °C	Open record: HRV 3C protease, MBP-LEVLFQ↓S-6His-GFP fusion protein (P1' residue Ser)
HRV 3C proteasevariant K22L/F25V/N106Y/T143M/Q145M/T129I	Human rhinovirus	MBP-LEVLFQ↓A-6His-GFP fusion protein (P1' residue Ala)	0.077	0.027	2.84	pH 8, 4 °C	Open record: HRV 3C protease variant K22L/F25V/N106Y/T143M/Q145M/T129I, MBP-LEVLFQ↓A-6His-GFP fusion protein (P1' residue Ala)
HRV 3C proteasevariant K22L/F25V/N106Y/T143M/Q145M/T129I	Human rhinovirus	MBP-LEVLFQ↓C-6His-GFP fusion protein (P1' residue Cys)	0.14	0.053	2.65	pH 8, 4 °C	Open record: HRV 3C protease variant K22L/F25V/N106Y/T143M/Q145M/T129I, MBP-LEVLFQ↓C-6His-GFP fusion protein (P1' residue Cys)
HRV 3C proteasevariant K22L/F25V/N106Y/T143M/Q145M/T129I	Human rhinovirus	MBP-LEVLFQ↓G-6His-GFP fusion protein (P1' residue Gly)	0.16	0.045	3.62	pH 8, 4 °C	Open record: HRV 3C protease variant K22L/F25V/N106Y/T143M/Q145M/T129I, MBP-LEVLFQ↓G-6His-GFP fusion protein (P1' residue Gly)
HRV 3C proteasevariant K22L/F25V/N106Y/T143M/Q145M/T129I	Human rhinovirus	MBP-LEVLFQ↓I-6His-GFP fusion protein (P1' residue Ile)	0.063	0.038	1.71	pH 8, 4 °C	Open record: HRV 3C protease variant K22L/F25V/N106Y/T143M/Q145M/T129I, MBP-LEVLFQ↓I-6His-GFP fusion protein (P1' residue Ile)
HRV 3C proteasevariant K22L/F25V/N106Y/T143M/Q145M/T129I	Human rhinovirus	MBP-LEVLFQ↓L-6His-GFP fusion protein (P1' residue Leu)	0.12	0.046	2.65	pH 8, 4 °C	Open record: HRV 3C protease variant K22L/F25V/N106Y/T143M/Q145M/T129I, MBP-LEVLFQ↓L-6His-GFP fusion protein (P1' residue Leu)
HRV 3C proteasevariant K22L/F25V/N106Y/T143M/Q145M/T129I	Human rhinovirus	MBP-LEVLFQ↓M-6His-GFP fusion protein (P1' residue Met)	0.18	0.048	3.72	pH 8, 4 °C	Open record: HRV 3C protease variant K22L/F25V/N106Y/T143M/Q145M/T129I, MBP-LEVLFQ↓M-6His-GFP fusion protein (P1' residue Met)
HRV 3C proteasevariant K22L/F25V/N106Y/T143M/Q145M/T129I	Human rhinovirus	MBP-LEVLFQ↓N-6His-GFP fusion protein (P1' residue Asn)	0.084	0.08	1.05	pH 8, 4 °C	Open record: HRV 3C protease variant K22L/F25V/N106Y/T143M/Q145M/T129I, MBP-LEVLFQ↓N-6His-GFP fusion protein (P1' residue Asn)
HRV 3C proteasevariant K22L/F25V/N106Y/T143M/Q145M/T129I	Human rhinovirus	MBP-LEVLFQ↓Q-6His-GFP fusion protein (P1' residue Gln)	0.033	0.089	0.37	pH 8, 4 °C	Open record: HRV 3C protease variant K22L/F25V/N106Y/T143M/Q145M/T129I, MBP-LEVLFQ↓Q-6His-GFP fusion protein (P1' residue Gln)
HRV 3C proteasevariant K22L/F25V/N106Y/T143M/Q145M/T129I	Human rhinovirus	MBP-LEVLFQ↓S-6His-GFP fusion protein (P1' residue Ser)	0.056	0.045	1.45	pH 8, 4 °C	Open record: HRV 3C protease variant K22L/F25V/N106Y/T143M/Q145M/T129I, MBP-LEVLFQ↓S-6His-GFP fusion protein (P1' residue Ser)
HRV 3C proteasevariant K22L/F25V/N106Y/T143M/Q145M/T129I	Human rhinovirus	MBP-LEVLFQ↓T-6His-GFP fusion protein (P1' residue Thr)	0.08	0.065	1.23	pH 8, 4 °C	Open record: HRV 3C protease variant K22L/F25V/N106Y/T143M/Q145M/T129I, MBP-LEVLFQ↓T-6His-GFP fusion protein (P1' residue Thr)