EC 2.7.7.48: RNA-directed RNA polymerase

TransferasesOpen in Browse

CatLog read 133 records for EC 2.7.7.48 from 9 papers, for 12 organisms. Names in these papers: NS2B-NS3 protease, HRV 3C protease, NS2B-NS3 protease (NS2B-NS3pro), nsp13 helicase and 6 more.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units.

CatLog also holds 47 records for EC 2.7.7.48 that are not yet re-read: from its earlier version, built from the enzyme databases and partly checked against the papers. All records for EC 2.7.7.48 in Browse.

Papers

Papers with records for EC 2.7.7.48
Paper	Organisms	Records
Insights to substrate binding and processing by West Nile Virus NS3 protease through combined modeling, protease mutagenesis, and kinetic studies	West Nile virus	71
A combinatorial strategy for HRV 3C protease engineering to achieve the N-terminal free cleavage	Human rhinovirus	14
Cysteine Disulfide Traps Reveal Distinct Conformational Ensembles in Dengue Virus NS2B-NS3 Protease	Dengue virus serotype 2	13
Motif V is an allosteric couple between the SARS-CoV-2 nsp13 nucleotide triphosphatase and helicase active sites	SARS-CoV-2	12
Remdesivir Is Effective in Combating COVID-19 because It Is a Better Substrate than ATP for the Viral RNA-Dependent RNA Polymerase	Severe acute respiratory syndrome coronavirus 2	6
An improved variant of tobacco etch virus (TEV) protease that does not need reducing agents	tobacco etch virus	6
Functional characterization of the NS2B/NS3 protease complex from seven viruses belonging to different groups inside the genus Flavivirus	Dengue virus type 1, Dengue virus type 3 and 1 more	5
Remdesivir is a direct-acting antiviral that inhibits RNA-dependent RNA polymerase from severe acute respiratory syndrome coronavirus 2 with high potency	Lassa virus, Severe acute respiratory syndrome coronavirus	4
The seven human coronavirus main proteases exhibit differential modulation of NF-κB suppression via TAB1 cleavage	human coronavirus NL63	2

Records

Records for EC 2.7.7.48 read from the paper
Enzyme	Organism	Substrate	k_cat (s⁻¹)	K_m (mM)	k_cat/K_m (mM⁻¹ s⁻¹)	Conditions	Paper	Open record
HCoV-NL63 Mpro	human coronavirus NL63	MCA-SLLQSEQQ-Lys(DNP)	0.804	0.1659	4.8	pH 7.4, 25 °C	PMID 42168570	Open record: HCoV-NL63 Mpro, MCA-SLLQSEQQ-Lys(DNP)
HCoV-NL63 Mpro	human coronavirus NL63	MCA-TAB1_{441-448}-Lys (DNP)	0.027	0.02129	1.3	pH 7.4, 25 °C	PMID 42168570	Open record: HCoV-NL63 Mpro, MCA-TAB1_{441-448}-Lys (DNP)
HRV 3C protease	Human rhinovirus	MBP-LEVLFQ↓A-6His-GFP fusion protein (P1' residue Ala)	0.032	0.034	0.91	pH 8, 4 °C	PMID 38521339	Open record: HRV 3C protease, MBP-LEVLFQ↓A-6His-GFP fusion protein (P1' residue Ala)
HRV 3C protease	Human rhinovirus	MBP-LEVLFQ↓C-6His-GFP fusion protein (P1' residue Cys)	0.021	0.045	0.47	pH 8, 4 °C	PMID 38521339	Open record: HRV 3C protease, MBP-LEVLFQ↓C-6His-GFP fusion protein (P1' residue Cys)
HRV 3C protease	Human rhinovirus	MBP-LEVLFQ↓G-6His-GFP fusion protein (P1' residue Gly)	0.22	0.047	4.73	pH 8, 4 °C	PMID 38521339	Open record: HRV 3C protease, MBP-LEVLFQ↓G-6His-GFP fusion protein (P1' residue Gly)
HRV 3C protease	Human rhinovirus	MBP-LEVLFQ↓S-6His-GFP fusion protein (P1' residue Ser)	0.021	0.049	0.39	pH 8, 4 °C	PMID 38521339	Open record: HRV 3C protease, MBP-LEVLFQ↓S-6His-GFP fusion protein (P1' residue Ser)
HRV 3C proteasevariant K22L/F25V/N106Y/T143M/Q145M/T129I	Human rhinovirus	MBP-LEVLFQ↓A-6His-GFP fusion protein (P1' residue Ala)	0.077	0.027	2.84	pH 8, 4 °C	PMID 38521339	Open record: HRV 3C protease variant K22L/F25V/N106Y/T143M/Q145M/T129I, MBP-LEVLFQ↓A-6His-GFP fusion protein (P1' residue Ala)
HRV 3C proteasevariant K22L/F25V/N106Y/T143M/Q145M/T129I	Human rhinovirus	MBP-LEVLFQ↓C-6His-GFP fusion protein (P1' residue Cys)	0.14	0.053	2.65	pH 8, 4 °C	PMID 38521339	Open record: HRV 3C protease variant K22L/F25V/N106Y/T143M/Q145M/T129I, MBP-LEVLFQ↓C-6His-GFP fusion protein (P1' residue Cys)
HRV 3C proteasevariant K22L/F25V/N106Y/T143M/Q145M/T129I	Human rhinovirus	MBP-LEVLFQ↓G-6His-GFP fusion protein (P1' residue Gly)	0.16	0.045	3.62	pH 8, 4 °C	PMID 38521339	Open record: HRV 3C protease variant K22L/F25V/N106Y/T143M/Q145M/T129I, MBP-LEVLFQ↓G-6His-GFP fusion protein (P1' residue Gly)
HRV 3C proteasevariant K22L/F25V/N106Y/T143M/Q145M/T129I	Human rhinovirus	MBP-LEVLFQ↓I-6His-GFP fusion protein (P1' residue Ile)	0.063	0.038	1.71	pH 8, 4 °C	PMID 38521339	Open record: HRV 3C protease variant K22L/F25V/N106Y/T143M/Q145M/T129I, MBP-LEVLFQ↓I-6His-GFP fusion protein (P1' residue Ile)
HRV 3C proteasevariant K22L/F25V/N106Y/T143M/Q145M/T129I	Human rhinovirus	MBP-LEVLFQ↓L-6His-GFP fusion protein (P1' residue Leu)	0.12	0.046	2.65	pH 8, 4 °C	PMID 38521339	Open record: HRV 3C protease variant K22L/F25V/N106Y/T143M/Q145M/T129I, MBP-LEVLFQ↓L-6His-GFP fusion protein (P1' residue Leu)
HRV 3C proteasevariant K22L/F25V/N106Y/T143M/Q145M/T129I	Human rhinovirus	MBP-LEVLFQ↓M-6His-GFP fusion protein (P1' residue Met)	0.18	0.048	3.72	pH 8, 4 °C	PMID 38521339	Open record: HRV 3C protease variant K22L/F25V/N106Y/T143M/Q145M/T129I, MBP-LEVLFQ↓M-6His-GFP fusion protein (P1' residue Met)
HRV 3C proteasevariant K22L/F25V/N106Y/T143M/Q145M/T129I	Human rhinovirus	MBP-LEVLFQ↓N-6His-GFP fusion protein (P1' residue Asn)	0.084	0.08	1.05	pH 8, 4 °C	PMID 38521339	Open record: HRV 3C protease variant K22L/F25V/N106Y/T143M/Q145M/T129I, MBP-LEVLFQ↓N-6His-GFP fusion protein (P1' residue Asn)
HRV 3C proteasevariant K22L/F25V/N106Y/T143M/Q145M/T129I	Human rhinovirus	MBP-LEVLFQ↓Q-6His-GFP fusion protein (P1' residue Gln)	0.033	0.089	0.37	pH 8, 4 °C	PMID 38521339	Open record: HRV 3C protease variant K22L/F25V/N106Y/T143M/Q145M/T129I, MBP-LEVLFQ↓Q-6His-GFP fusion protein (P1' residue Gln)
HRV 3C proteasevariant K22L/F25V/N106Y/T143M/Q145M/T129I	Human rhinovirus	MBP-LEVLFQ↓S-6His-GFP fusion protein (P1' residue Ser)	0.056	0.045	1.45	pH 8, 4 °C	PMID 38521339	Open record: HRV 3C protease variant K22L/F25V/N106Y/T143M/Q145M/T129I, MBP-LEVLFQ↓S-6His-GFP fusion protein (P1' residue Ser)
HRV 3C proteasevariant K22L/F25V/N106Y/T143M/Q145M/T129I	Human rhinovirus	MBP-LEVLFQ↓T-6His-GFP fusion protein (P1' residue Thr)	0.08	0.065	1.23	pH 8, 4 °C	PMID 38521339	Open record: HRV 3C protease variant K22L/F25V/N106Y/T143M/Q145M/T129I, MBP-LEVLFQ↓T-6His-GFP fusion protein (P1' residue Thr)
NS2B-NS3 protease	West Nile virus	2-Naph-Lys-AcLys-Arg-pNA	0.3	2.303	0.131	pH 9.5, 37 °C	PMID 17052977	Open record: NS2B-NS3 protease, 2-Naph-Lys-AcLys-Arg-pNA
NS2B-NS3 protease	West Nile virus	2-naphthoyl-3-pyridylalanyl-L-lysyl-L-arginine para-nitroanilide	0.96	0.0658	14.62	pH 9.5, 37 °C	PMID 17052977	Open record: NS2B-NS3 protease, 2-naphthoyl-3-pyridylalanyl-L-lysyl-L-arginine para-nitroanilide
NS2B-NS3 protease	West Nile virus	2-naphthoyl-L-citrullyl-L-lysyl-L-arginine para-nitroanilide	0.32	0.021	15.15	pH 9.5, 37 °C	PMID 17052977	Open record: NS2B-NS3 protease, 2-naphthoyl-L-citrullyl-L-lysyl-L-arginine para-nitroanilide
NS2B-NS3 protease	West Nile virus	2-naphthoyl-L-citrullyl-L-ornithyl-L-arginine para-nitroanilide	0.57	0.1007	5.612	pH 9.5, 37 °C	PMID 17052977	Open record: NS2B-NS3 protease, 2-naphthoyl-L-citrullyl-L-ornithyl-L-arginine para-nitroanilide
NS2B-NS3 protease	West Nile virus	2-naphthoyl-L-homoarginyl-L-lysyl-L-arginine para-nitroanilide	0.11	0.048	2.294	pH 9.5, 37 °C	PMID 17052977	Open record: NS2B-NS3 protease, 2-naphthoyl-L-homoarginyl-L-lysyl-L-arginine para-nitroanilide
NS2B-NS3 protease	West Nile virus	2-naphthoyl-L-lysyl-L-arginyl-L-arginine para-nitroanilide	0.885	0.0434	20.38	pH 9.5, 37 °C	PMID 17052977	Open record: NS2B-NS3 protease, 2-naphthoyl-L-lysyl-L-arginyl-L-arginine para-nitroanilide
NS2B-NS3 protease	West Nile virus	2-naphthoyl-L-lysyl-L-citrullyl-L-arginine para-nitroanilide	0.43	3.423	0.127	pH 9.5, 37 °C	PMID 17052977	Open record: NS2B-NS3 protease, 2-naphthoyl-L-lysyl-L-citrullyl-L-arginine para-nitroanilide
NS2B-NS3 protease	West Nile virus	2-naphthoyl-L-lysyl-L-homoarginyl-L-arginine para-nitroanilide	0.59	0.0267	22.1	pH 9.5, 37 °C	PMID 17052977	Open record: NS2B-NS3 protease, 2-naphthoyl-L-lysyl-L-homoarginyl-L-arginine para-nitroanilide
NS2B-NS3 protease	West Nile virus	2-naphthoyl-L-lysyl-L-lysyl-L-arginine para-nitroanilide	1.083	0.0254	42.60	pH 9.5, 37 °C	PMID 17052977	Open record: NS2B-NS3 protease, 2-naphthoyl-L-lysyl-L-lysyl-L-arginine para-nitroanilide
NS2B-NS3 protease	West Nile virus	2-naphthoyl-L-lysyl-L-ornithyl-L-arginine para-nitroanilide	1.58	0.0368	42.89	pH 9.5, 37 °C	PMID 17052977	Open record: NS2B-NS3 protease, 2-naphthoyl-L-lysyl-L-ornithyl-L-arginine para-nitroanilide
NS2B-NS3 protease	West Nile virus	2-naphthoyl-L-ornithyl-L-lysyl-L-arginine para-nitroanilide	0.98	0.038	25.74	pH 9.5, 37 °C	PMID 17052977	Open record: NS2B-NS3 protease, 2-naphthoyl-L-ornithyl-L-lysyl-L-arginine para-nitroanilide
NS2B-NS3 protease	West Nile virus	2-naphthoyl-L-ornithyl-L-ornithyl-L-arginine para-nitroanilide	0.27	0.0229	11.90	pH 9.5, 37 °C	PMID 17052977	Open record: NS2B-NS3 protease, 2-naphthoyl-L-ornithyl-L-ornithyl-L-arginine para-nitroanilide
NS2B-NS3 protease	West Nile virus	N-acetyl-2-aminoisobutyryl-L-lysyl-L-lysyl-L-arginine para-nitroanilide	1.6	0.0797	20.05	pH 9.5, 37 °C	PMID 17052977	Open record: NS2B-NS3 protease, N-acetyl-2-aminoisobutyryl-L-lysyl-L-lysyl-L-arginine para-nitroanilide
NS2B-NS3 protease	West Nile virus	N-acetyl-L-2-aminooctanoyl-L-lysyl-L-lysyl-L-arginine para-nitroanilide	2.51	0.0755	33.19	pH 9.5, 37 °C	PMID 17052977	Open record: NS2B-NS3 protease, N-acetyl-L-2-aminooctanoyl-L-lysyl-L-lysyl-L-arginine para-nitroanilide
NS2B-NS3 protease	West Nile virus	N-acetyl-L-cyclohexylalanyl-L-lysyl-L-lysyl-L-arginine para-nitroanilide	2.28	0.0699	32.60	pH 9.5, 37 °C	PMID 17052977	Open record: NS2B-NS3 protease, N-acetyl-L-cyclohexylalanyl-L-lysyl-L-lysyl-L-arginine para-nitroanilide
NS2B-NS3 protease	West Nile virus	N-acetyl-L-cyclohexylglycyl-L-lysyl-L-lysyl-L-arginine para-nitroanilide	1.78	0.0819	21.73	pH 9.5, 37 °C	PMID 17052977	Open record: NS2B-NS3 protease, N-acetyl-L-cyclohexylglycyl-L-lysyl-L-lysyl-L-arginine para-nitroanilide
NS2B-NS3 protease	West Nile virus	N-acetyl-L-leucyl-3-pyridylalanyl-L-lysyl-L-arginine para-nitroanilide	0.5	0.0887	5.601	pH 9.5, 37 °C	PMID 17052977	Open record: NS2B-NS3 protease, N-acetyl-L-leucyl-3-pyridylalanyl-L-lysyl-L-arginine para-nitroanilide
NS2B-NS3 protease	West Nile virus	N-acetyl-L-leucyl-4-amino-L-phenylalanyl-L-lysyl-L-arginine para-nitroanilide	0.75	0.1413	5.306	pH 9.5, 37 °C	PMID 17052977	Open record: NS2B-NS3 protease, N-acetyl-L-leucyl-4-amino-L-phenylalanyl-L-lysyl-L-arginine para-nitroanilide
NS2B-NS3 protease	West Nile virus	N-acetyl-L-leucyl-4-nitro-L-phenylalanyl-L-lysyl-L-arginine para-nitroanilide	0.72	0.2541	2.817	pH 9.5, 37 °C	PMID 17052977	Open record: NS2B-NS3 protease, N-acetyl-L-leucyl-4-nitro-L-phenylalanyl-L-lysyl-L-arginine para-nitroanilide
NS2B-NS3 protease	West Nile virus	N-acetyl-L-leucyl-4-pyridylalanyl-L-lysyl-L-arginine para-nitroanilide	0.9	0.2548	3.536	pH 9.5, 37 °C	PMID 17052977	Open record: NS2B-NS3 protease, N-acetyl-L-leucyl-4-pyridylalanyl-L-lysyl-L-arginine para-nitroanilide
NS2B-NS3 protease	West Nile virus	N-acetyl-L-leucyl-L-citrullyl-L-lysyl-L-arginine para-nitroanilide	1.44	0.2086	6.904	pH 9.5, 37 °C	PMID 17052977	Open record: NS2B-NS3 protease, N-acetyl-L-leucyl-L-citrullyl-L-lysyl-L-arginine para-nitroanilide
NS2B-NS3 protease	West Nile virus	N-acetyl-L-leucyl-L-glutaminyl-L-tyrosyl-L-threonyl-L-lysyl-L-arginine para-nitroanilide	0.266	0.199	1.332	pH 9.5, 37 °C	PMID 17052977	Open record: NS2B-NS3 protease, N-acetyl-L-leucyl-L-glutaminyl-L-tyrosyl-L-threonyl-L-lysyl-L-arginine para-nitroanilide
NS2B-NS3 protease	West Nile virus	N-acetyl-L-leucyl-L-homoarginyl-L-lysyl-L-arginine para-nitroanilide	1.18	0.0625	18.96	pH 9.5, 37 °C	PMID 17052977	Open record: NS2B-NS3 protease, N-acetyl-L-leucyl-L-homoarginyl-L-lysyl-L-arginine para-nitroanilide
NS2B-NS3 protease	West Nile virus	N-acetyl-L-leucyl-L-lysyl-L-arginyl-L-arginine para-nitroanilide	2.494	0.195	12.78	pH 9.5, 37 °C	PMID 17052977	Open record: NS2B-NS3 protease, N-acetyl-L-leucyl-L-lysyl-L-arginyl-L-arginine para-nitroanilide
NS2B-NS3 protease	West Nile virus	N-acetyl-L-leucyl-L-lysyl-L-lysyl-L-arginine para-nitroanilide	1.644	0.0546	30.12	pH 9.5, 37 °C	PMID 17052977	Open record: NS2B-NS3 protease, N-acetyl-L-leucyl-L-lysyl-L-lysyl-L-arginine para-nitroanilide
NS2B-NS3 protease	West Nile virus	N-acetyl-L-leucyl-L-ornithyl-L-lysyl-L-arginine para-nitroanilide	1.79	0.1055	17.00	pH 9.5, 37 °C	PMID 17052977	Open record: NS2B-NS3 protease, N-acetyl-L-leucyl-L-ornithyl-L-lysyl-L-arginine para-nitroanilide
NS2B-NS3 protease	West Nile virus	N-acetyl-L-norleucyl-L-lysyl-L-lysyl-L-arginine para-nitroanilide	2.35	0.0807	29.09	pH 9.5, 37 °C	PMID 17052977	Open record: NS2B-NS3 protease, N-acetyl-L-norleucyl-L-lysyl-L-lysyl-L-arginine para-nitroanilide
NS2B-NS3 protease	West Nile virus	N-acetyl-L-norvalyl-L-lysyl-L-lysyl-L-arginine para-nitroanilide	2.36	0.0673	35.00	pH 9.5, 37 °C	PMID 17052977	Open record: NS2B-NS3 protease, N-acetyl-L-norvalyl-L-lysyl-L-lysyl-L-arginine para-nitroanilide
NS2B-NS3 protease	West Nile virus	N-acetyl-L-tert-butylglycyl-L-lysyl-L-lysyl-L-arginine para-nitroanilide	1.74	0.1342	12.96	pH 9.5, 37 °C	PMID 17052977	Open record: NS2B-NS3 protease, N-acetyl-L-tert-butylglycyl-L-lysyl-L-lysyl-L-arginine para-nitroanilide
NS2B-NS3 proteasevariant NS2B-L87A	West Nile virus	2-naphthoyl-L-lysyl-L-lysyl-L-arginine para-nitroanilide	0.0478	0.0884	0.541	pH 9.5, 37 °C	PMID 17052977	Open record: NS2B-NS3 protease variant NS2B-L87A, 2-naphthoyl-L-lysyl-L-lysyl-L-arginine para-nitroanilide
NS2B-NS3 proteasevariant NS2B-L87A	West Nile virus	N-acetyl-L-leucyl-L-lysyl-L-lysyl-L-arginine para-nitroanilide	0.07	0.434	0.161	pH 9.5, 37 °C	PMID 17052977	Open record: NS2B-NS3 protease variant NS2B-L87A, N-acetyl-L-leucyl-L-lysyl-L-lysyl-L-arginine para-nitroanilide
NS2B-NS3 proteasevariant NS2B-L87F	West Nile virus	2-naphthoyl-L-lysyl-L-lysyl-L-arginine para-nitroanilide	0.288	0.0369	7.796	pH 9.5, 37 °C	PMID 17052977	Open record: NS2B-NS3 protease variant NS2B-L87F, 2-naphthoyl-L-lysyl-L-lysyl-L-arginine para-nitroanilide
NS2B-NS3 proteasevariant NS2B-L87F	West Nile virus	N-acetyl-L-leucyl-L-glutaminyl-L-tyrosyl-L-threonyl-L-lysyl-L-arginine para-nitroanilide	0.228	0.764	0.299	pH 9.5, 37 °C	PMID 17052977	Open record: NS2B-NS3 protease variant NS2B-L87F, N-acetyl-L-leucyl-L-glutaminyl-L-tyrosyl-L-threonyl-L-lysyl-L-arginine para-nitroanilide
NS2B-NS3 proteasevariant NS2B-L87F	West Nile virus	N-acetyl-L-leucyl-L-lysyl-L-lysyl-L-arginine para-nitroanilide	1.342	0.144	9.345	pH 9.5, 37 °C	PMID 17052977	Open record: NS2B-NS3 protease variant NS2B-L87F, N-acetyl-L-leucyl-L-lysyl-L-lysyl-L-arginine para-nitroanilide
NS2B-NS3 proteasevariant NS2B-N84A	West Nile virus	N-acetyl-L-leucyl-L-lysyl-L-lysyl-L-arginine para-nitroanilide	0.126	0.376	0.336	pH 9.5, 37 °C	PMID 17052977	Open record: NS2B-NS3 protease variant NS2B-N84A, N-acetyl-L-leucyl-L-lysyl-L-lysyl-L-arginine para-nitroanilide
NS2B-NS3 proteasevariant NS2B-N84D	West Nile virus	N-acetyl-L-leucyl-L-lysyl-L-arginyl-L-arginine para-nitroanilide	0.398	0.0698	5.698	pH 9.5, 37 °C	PMID 17052977	Open record: NS2B-NS3 protease variant NS2B-N84D, N-acetyl-L-leucyl-L-lysyl-L-arginyl-L-arginine para-nitroanilide
NS2B-NS3 proteasevariant NS2B-N84D	West Nile virus	N-acetyl-L-leucyl-L-lysyl-L-lysyl-L-arginine para-nitroanilide	1.532	0.117	13.11	pH 9.5, 37 °C	PMID 17052977	Open record: NS2B-NS3 protease variant NS2B-N84D, N-acetyl-L-leucyl-L-lysyl-L-lysyl-L-arginine para-nitroanilide
NS2B-NS3 proteasevariant NS2B-N84E	West Nile virus	N-acetyl-L-leucyl-L-lysyl-L-arginyl-L-arginine para-nitroanilide	2.707	0.0664	40.80	pH 9.5, 37 °C	PMID 17052977	Open record: NS2B-NS3 protease variant NS2B-N84E, N-acetyl-L-leucyl-L-lysyl-L-arginyl-L-arginine para-nitroanilide
NS2B-NS3 proteasevariant NS2B-N84E	West Nile virus	N-acetyl-L-leucyl-L-lysyl-L-lysyl-L-arginine para-nitroanilide	3.027	0.0793	38.19	pH 9.5, 37 °C	PMID 17052977	Open record: NS2B-NS3 protease variant NS2B-N84E, N-acetyl-L-leucyl-L-lysyl-L-lysyl-L-arginine para-nitroanilide
NS2B-NS3 proteasevariant NS2B-N84L	West Nile virus	N-acetyl-L-leucyl-L-lysyl-L-lysyl-L-arginine para-nitroanilide	0.34	0.22	1.543	pH 9.5, 37 °C	PMID 17052977	Open record: NS2B-NS3 protease variant NS2B-N84L, N-acetyl-L-leucyl-L-lysyl-L-lysyl-L-arginine para-nitroanilide
NS2B-NS3 proteasevariant NS2B-N84S	West Nile virus	2-naphthoyl-L-lysyl-L-arginyl-L-arginine para-nitroanilide	0.151	0.0197	7.695	pH 9.5, 37 °C	PMID 17052977	Open record: NS2B-NS3 protease variant NS2B-N84S, 2-naphthoyl-L-lysyl-L-arginyl-L-arginine para-nitroanilide
NS2B-NS3 proteasevariant NS2B-N84S	West Nile virus	2-naphthoyl-L-lysyl-L-homoarginyl-L-arginine para-nitroanilide	0.027	0.0295	0.904	pH 9.5, 37 °C	PMID 17052977	Open record: NS2B-NS3 protease variant NS2B-N84S, 2-naphthoyl-L-lysyl-L-homoarginyl-L-arginine para-nitroanilide
NS2B-NS3 proteasevariant NS2B-N84S	West Nile virus	N-acetyl-L-leucyl-L-lysyl-L-arginyl-L-arginine para-nitroanilide	0.158	0.1	1.577	pH 9.5, 37 °C	PMID 17052977	Open record: NS2B-NS3 protease variant NS2B-N84S, N-acetyl-L-leucyl-L-lysyl-L-arginyl-L-arginine para-nitroanilide
NS2B-NS3 proteasevariant NS2B-N84S	West Nile virus	N-acetyl-L-leucyl-L-lysyl-L-lysyl-L-arginine para-nitroanilide	0.682	0.205	3.33	pH 9.5, 37 °C	PMID 17052977	Open record: NS2B-NS3 protease variant NS2B-N84S, N-acetyl-L-leucyl-L-lysyl-L-lysyl-L-arginine para-nitroanilide
NS2B-NS3 proteasevariant NS2B-Q86A	West Nile virus	N-acetyl-L-leucyl-L-glutaminyl-L-tyrosyl-L-threonyl-L-lysyl-L-arginine para-nitroanilide	0.127	1.203	0.105	pH 9.5, 37 °C	PMID 17052977	Open record: NS2B-NS3 protease variant NS2B-Q86A, N-acetyl-L-leucyl-L-glutaminyl-L-tyrosyl-L-threonyl-L-lysyl-L-arginine para-nitroanilide
NS2B-NS3 proteasevariant NS2B-Q86A	West Nile virus	N-acetyl-L-leucyl-L-lysyl-L-lysyl-L-arginine para-nitroanilide	0.367	0.258	1.424	pH 9.5, 37 °C	PMID 17052977	Open record: NS2B-NS3 protease variant NS2B-Q86A, N-acetyl-L-leucyl-L-lysyl-L-lysyl-L-arginine para-nitroanilide
NS2B-NS3 proteasevariant NS2B-Q86E	West Nile virus	N-acetyl-L-leucyl-L-lysyl-L-lysyl-L-arginine para-nitroanilide	0.868	0.0777	11.18	pH 9.5, 37 °C	PMID 17052977	Open record: NS2B-NS3 protease variant NS2B-Q86E, N-acetyl-L-leucyl-L-lysyl-L-lysyl-L-arginine para-nitroanilide
NS2B-NS3 proteasevariant NS2B-Q86L	West Nile virus	N-acetyl-L-leucyl-L-lysyl-L-lysyl-L-arginine para-nitroanilide	0.973	0.148	6.586	pH 9.5, 37 °C	PMID 17052977	Open record: NS2B-NS3 protease variant NS2B-Q86L, N-acetyl-L-leucyl-L-lysyl-L-lysyl-L-arginine para-nitroanilide
NS2B-NS3 proteasevariant NS2B-V75A	West Nile virus	N-acetyl-L-leucyl-L-glutaminyl-L-tyrosyl-L-threonyl-L-lysyl-L-arginine para-nitroanilide	0.164	5.318	0.031	pH 9.5, 37 °C	PMID 17052977	Open record: NS2B-NS3 protease variant NS2B-V75A, N-acetyl-L-leucyl-L-glutaminyl-L-tyrosyl-L-threonyl-L-lysyl-L-arginine para-nitroanilide
NS2B-NS3 proteasevariant NS2B-V75A	West Nile virus	N-acetyl-L-leucyl-L-lysyl-L-lysyl-L-arginine para-nitroanilide	0.173	0.199	0.866	pH 9.5, 37 °C	PMID 17052977	Open record: NS2B-NS3 protease variant NS2B-V75A, N-acetyl-L-leucyl-L-lysyl-L-lysyl-L-arginine para-nitroanilide
NS2B-NS3 proteasevariant NS2B-V75F	West Nile virus	N-acetyl-L-leucyl-L-glutaminyl-L-tyrosyl-L-threonyl-L-lysyl-L-arginine para-nitroanilide	0.091	0.809	0.113	pH 9.5, 37 °C	PMID 17052977	Open record: NS2B-NS3 protease variant NS2B-V75F, N-acetyl-L-leucyl-L-glutaminyl-L-tyrosyl-L-threonyl-L-lysyl-L-arginine para-nitroanilide
NS2B-NS3 proteasevariant NS2B-V75F	West Nile virus	N-acetyl-L-leucyl-L-lysyl-L-lysyl-L-arginine para-nitroanilide	0.246	0.272	0.905	pH 9.5, 37 °C	PMID 17052977	Open record: NS2B-NS3 protease variant NS2B-V75F, N-acetyl-L-leucyl-L-lysyl-L-lysyl-L-arginine para-nitroanilide
NS2B-NS3 proteasevariant NS3-A164S	West Nile virus	N-acetyl-L-leucyl-L-lysyl-L-lysyl-L-arginine para-nitroanilide	0.26	0.221	1.178	pH 9.5, 37 °C	PMID 17052977	Open record: NS2B-NS3 protease variant NS3-A164S, N-acetyl-L-leucyl-L-lysyl-L-lysyl-L-arginine para-nitroanilide
NS2B-NS3 proteasevariant NS3-A164V	West Nile virus	N-acetyl-L-leucyl-L-lysyl-L-lysyl-L-arginine para-nitroanilide	0.181	0.331	0.546	pH 9.5, 37 °C	PMID 17052977	Open record: NS2B-NS3 protease variant NS3-A164V, N-acetyl-L-leucyl-L-lysyl-L-lysyl-L-arginine para-nitroanilide
NS2B-NS3 proteasevariant NS3-D129A	West Nile virus	N-acetyl-L-leucyl-L-lysyl-L-lysyl-L-arginine para-nitroanilide	0.048	0.635	0.075	pH 9.5, 37 °C	PMID 17052977	Open record: NS2B-NS3 protease variant NS3-D129A, N-acetyl-L-leucyl-L-lysyl-L-lysyl-L-arginine para-nitroanilide
NS2B-NS3 proteasevariant NS3-D129E	West Nile virus	N-acetyl-L-leucyl-L-lysyl-L-lysyl-L-arginine para-nitroanilide	0.036	0.432	0.083	pH 9.5, 37 °C	PMID 17052977	Open record: NS2B-NS3 protease variant NS3-D129E, N-acetyl-L-leucyl-L-lysyl-L-lysyl-L-arginine para-nitroanilide
NS2B-NS3 proteasevariant NS3-I155F	West Nile virus	N-acetyl-L-leucyl-L-lysyl-L-lysyl-L-arginine para-nitroanilide	0.733	0.207	3.532	pH 9.5, 37 °C	PMID 17052977	Open record: NS2B-NS3 protease variant NS3-I155F, N-acetyl-L-leucyl-L-lysyl-L-lysyl-L-arginine para-nitroanilide
NS2B-NS3 proteasevariant NS3-I162F	West Nile virus	N-acetyl-L-leucyl-L-glutaminyl-L-tyrosyl-L-threonyl-L-lysyl-L-arginine para-nitroanilide	0.04	0.416	0.095	pH 9.5, 37 °C	PMID 17052977	Open record: NS2B-NS3 protease variant NS3-I162F, N-acetyl-L-leucyl-L-glutaminyl-L-tyrosyl-L-threonyl-L-lysyl-L-arginine para-nitroanilide
NS2B-NS3 proteasevariant NS3-I162F	West Nile virus	N-acetyl-L-leucyl-L-lysyl-L-lysyl-L-arginine para-nitroanilide	0.164	0.268	0.612	pH 9.5, 37 °C	PMID 17052977	Open record: NS2B-NS3 protease variant NS3-I162F, N-acetyl-L-leucyl-L-lysyl-L-lysyl-L-arginine para-nitroanilide
NS2B-NS3 proteasevariant NS3-M156A	West Nile virus	N-acetyl-L-leucyl-L-glutaminyl-L-tyrosyl-L-threonyl-L-lysyl-L-arginine para-nitroanilide	0.314	0.879	0.357	pH 9.5, 37 °C	PMID 17052977	Open record: NS2B-NS3 protease variant NS3-M156A, N-acetyl-L-leucyl-L-glutaminyl-L-tyrosyl-L-threonyl-L-lysyl-L-arginine para-nitroanilide
NS2B-NS3 proteasevariant NS3-M156A	West Nile virus	N-acetyl-L-leucyl-L-lysyl-L-lysyl-L-arginine para-nitroanilide	0.778	0.164	4.741	pH 9.5, 37 °C	PMID 17052977	Open record: NS2B-NS3 protease variant NS3-M156A, N-acetyl-L-leucyl-L-lysyl-L-lysyl-L-arginine para-nitroanilide
NS2B-NS3 proteasevariant NS3-T111F	West Nile virus	N-acetyl-L-leucyl-L-glutaminyl-L-tyrosyl-L-threonyl-L-lysyl-L-arginine para-nitroanilide	0.051	0.376	0.135	pH 9.5, 37 °C	PMID 17052977	Open record: NS2B-NS3 protease variant NS3-T111F, N-acetyl-L-leucyl-L-glutaminyl-L-tyrosyl-L-threonyl-L-lysyl-L-arginine para-nitroanilide
NS2B-NS3 proteasevariant NS3-T111F	West Nile virus	N-acetyl-L-leucyl-L-lysyl-L-lysyl-L-arginine para-nitroanilide	0.198	0.169	1.176	pH 9.5, 37 °C	PMID 17052977	Open record: NS2B-NS3 protease variant NS3-T111F, N-acetyl-L-leucyl-L-lysyl-L-lysyl-L-arginine para-nitroanilide
NS2B-NS3 proteasevariant NS3-T111L	West Nile virus	N-acetyl-L-leucyl-L-glutaminyl-L-tyrosyl-L-threonyl-L-lysyl-L-arginine para-nitroanilide	0.018	0.617	0.03	pH 9.5, 37 °C	PMID 17052977	Open record: NS2B-NS3 protease variant NS3-T111L, N-acetyl-L-leucyl-L-glutaminyl-L-tyrosyl-L-threonyl-L-lysyl-L-arginine para-nitroanilide
NS2B-NS3 proteasevariant NS3-T111L	West Nile virus	N-acetyl-L-leucyl-L-lysyl-L-lysyl-L-arginine para-nitroanilide	0.06	0.214	0.279	pH 9.5, 37 °C	PMID 17052977	Open record: NS2B-NS3 protease variant NS3-T111L, N-acetyl-L-leucyl-L-lysyl-L-lysyl-L-arginine para-nitroanilide
NS2B-NS3 proteasevariant NS3-V154F	West Nile virus	2-naphthoyl-L-lysyl-L-lysyl-L-arginine para-nitroanilide	0.0431	0.0977	0.442	pH 9.5, 37 °C	PMID 17052977	Open record: NS2B-NS3 protease variant NS3-V154F, 2-naphthoyl-L-lysyl-L-lysyl-L-arginine para-nitroanilide
NS2B-NS3 proteasevariant NS3-V154F	West Nile virus	N-acetyl-L-leucyl-L-lysyl-L-lysyl-L-arginine para-nitroanilide	0.057	0.263	0.216	pH 9.5, 37 °C	PMID 17052977	Open record: NS2B-NS3 protease variant NS3-V154F, N-acetyl-L-leucyl-L-lysyl-L-lysyl-L-arginine para-nitroanilide
NS2B-NS3 proteasevariant NS3-V154L	West Nile virus	N-acetyl-L-leucyl-L-glutaminyl-L-tyrosyl-L-threonyl-L-lysyl-L-arginine para-nitroanilide	0.245	0.344	0.712	pH 9.5, 37 °C	PMID 17052977	Open record: NS2B-NS3 protease variant NS3-V154L, N-acetyl-L-leucyl-L-glutaminyl-L-tyrosyl-L-threonyl-L-lysyl-L-arginine para-nitroanilide
NS2B-NS3 proteasevariant NS3-V154L	West Nile virus	N-acetyl-L-leucyl-L-lysyl-L-lysyl-L-arginine para-nitroanilide	0.8	0.19	4.223	pH 9.5, 37 °C	PMID 17052977	Open record: NS2B-NS3 protease variant NS3-V154L, N-acetyl-L-leucyl-L-lysyl-L-lysyl-L-arginine para-nitroanilide
NS2B-NS3 proteasevariant NS3-V166L	West Nile virus	N-acetyl-L-leucyl-L-glutaminyl-L-tyrosyl-L-threonyl-L-lysyl-L-arginine para-nitroanilide	0.297	0.232	1.282	pH 9.5, 37 °C	PMID 17052977	Open record: NS2B-NS3 protease variant NS3-V166L, N-acetyl-L-leucyl-L-glutaminyl-L-tyrosyl-L-threonyl-L-lysyl-L-arginine para-nitroanilide
NS2B-NS3 proteasevariant NS3-V166L	West Nile virus	N-acetyl-L-leucyl-L-lysyl-L-lysyl-L-arginine para-nitroanilide	0.638	0.096	6.676	pH 9.5, 37 °C	PMID 17052977	Open record: NS2B-NS3 protease variant NS3-V166L, N-acetyl-L-leucyl-L-lysyl-L-lysyl-L-arginine para-nitroanilide
NS2B-NS3 protease (NS2B-NS3pro)	Dengue virus serotype 2	N-acetyl-Gly-Arg-Arg-7-amino-4-methylcoumarinN-terminally His6-tagged single chain	0.05	0.07	0.7	pH 8.5, 37 °C	PMID 30472839	Open record: NS2B-NS3 protease (NS2B-NS3pro), N-acetyl-Gly-Arg-Arg-7-amino-4-methylcoumarin
NS2B-NS3 protease (NS2B-NS3pro)	Dengue virus serotype 2	N-acetyl-Gly-Arg-Arg-7-amino-4-methylcoumarin	0.09	0.04	2	pH 8.5, 37 °C	PMID 30472839	Open record: NS2B-NS3 protease (NS2B-NS3pro), N-acetyl-Gly-Arg-Arg-7-amino-4-methylcoumarin
NS2B-NS3 protease (NS2B-NS3pro)variant A125C/V162C	Dengue virus serotype 2	N-acetyl-Gly-Arg-Arg-7-amino-4-methylcoumarinN-terminally His6-tagged single chain	0.02	0.03	0.8	pH 8.5, 37 °C	PMID 30472839	Open record: NS2B-NS3 protease (NS2B-NS3pro) variant A125C/V162C, N-acetyl-Gly-Arg-Arg-7-amino-4-methylcoumarin
NS2B-NS3 protease (NS2B-NS3pro)variant A125C/V162C	Dengue virus serotype 2	N-acetyl-Gly-Arg-Arg-7-amino-4-methylcoumarin	0.01	0.05	0.3	pH 8.5, 37 °C	PMID 30472839	Open record: NS2B-NS3 protease (NS2B-NS3pro) variant A125C/V162C, N-acetyl-Gly-Arg-Arg-7-amino-4-methylcoumarin
NS2B-NS3 protease (NS2B-NS3pro)variant I73C*/P106C	Dengue virus serotype 2	N-acetyl-Gly-Arg-Arg-7-amino-4-methylcoumarinN-terminally His6-tagged single chain	0.03	0.04	0.8	pH 8.5, 37 °C	PMID 30472839	Open record: NS2B-NS3 protease (NS2B-NS3pro) variant I73C*/P106C, N-acetyl-Gly-Arg-Arg-7-amino-4-methylcoumarin
NS2B-NS3 protease (NS2B-NS3pro)variant I73C*/P106C	Dengue virus serotype 2	N-acetyl-Gly-Arg-Arg-7-amino-4-methylcoumarin	0.08	0.05	2	pH 8.5, 37 °C	PMID 30472839	Open record: NS2B-NS3 protease (NS2B-NS3pro) variant I73C*/P106C, N-acetyl-Gly-Arg-Arg-7-amino-4-methylcoumarin
NS2B-NS3 protease (NS2B-NS3pro)variant K117A	Dengue virus serotype 2	N-acetyl-Gly-Arg-Arg-7-amino-4-methylcoumarinN-terminally His6-tagged single chain	0.006	0.04	0.2	pH 8.5, 37 °C	PMID 30472839	Open record: NS2B-NS3 protease (NS2B-NS3pro) variant K117A, N-acetyl-Gly-Arg-Arg-7-amino-4-methylcoumarin
NS2B-NS3 protease (NS2B-NS3pro)variant K117A	Dengue virus serotype 2	N-acetyl-Gly-Arg-Arg-7-amino-4-methylcoumarin	0.05	0.08	0.7	pH 8.5, 37 °C	PMID 30472839	Open record: NS2B-NS3 protease (NS2B-NS3pro) variant K117A, N-acetyl-Gly-Arg-Arg-7-amino-4-methylcoumarin
NS2B-NS3 protease (NS2B-NS3pro)variant K117C	Dengue virus serotype 2	N-acetyl-Gly-Arg-Arg-7-amino-4-methylcoumarin	0.02	0.05	0.44	pH 8.5, 37 °C	PMID 30472839	Open record: NS2B-NS3 protease (NS2B-NS3pro) variant K117C, N-acetyl-Gly-Arg-Arg-7-amino-4-methylcoumarin
NS2B-NS3 protease (NS2B-NS3pro)variant K117R	Dengue virus serotype 2	N-acetyl-Gly-Arg-Arg-7-amino-4-methylcoumarin	0.04	0.05	0.8	pH 8.5, 37 °C	PMID 30472839	Open record: NS2B-NS3 protease (NS2B-NS3pro) variant K117R, N-acetyl-Gly-Arg-Arg-7-amino-4-methylcoumarin
NS2B-NS3 protease (NS2B-NS3pro)variant S75C*	Dengue virus serotype 2	N-acetyl-Gly-Arg-Arg-7-amino-4-methylcoumarin	0.05	0.04	1	pH 8.5, 37 °C	PMID 30472839	Open record: NS2B-NS3 protease (NS2B-NS3pro) variant S75C*, N-acetyl-Gly-Arg-Arg-7-amino-4-methylcoumarin
NS2B-NS3 protease (NS2B-NS3pro)variant S75C*/K117C	Dengue virus serotype 2	N-acetyl-Gly-Arg-Arg-7-amino-4-methylcoumarinN-terminally His6-tagged single chain	0.02	0.03	0.8	pH 8.5, 37 °C	PMID 30472839	Open record: NS2B-NS3 protease (NS2B-NS3pro) variant S75C*/K117C, N-acetyl-Gly-Arg-Arg-7-amino-4-methylcoumarin
NS2B-NS3 protease (NS2B-NS3pro)variant S75C*/K117C	Dengue virus serotype 2	N-acetyl-Gly-Arg-Arg-7-amino-4-methylcoumarin	0.07	0.04	2	pH 8.5, 37 °C	PMID 30472839	Open record: NS2B-NS3 protease (NS2B-NS3pro) variant S75C*/K117C, N-acetyl-Gly-Arg-Arg-7-amino-4-methylcoumarin
nsp13 helicase	SARS-CoV-2	ATP100 nM labeled dsDNA	1.4	0.122	13	pH 7.5, 37 °C	PMID 41581884	Open record: nsp13 helicase, ATP
nsp13 helicase	SARS-CoV-2	ATPunlabeled dsDNA present	0.37	0.156	2.7	pH 7.5, 37 °C	PMID 41581884	Open record: nsp13 helicase, ATP
nsp13 helicase	SARS-CoV-2	dsDNA	1.5	2.85 × 10⁻⁴	6 × 10³	pH 7.5, 37 °C	PMID 41581884	Open record: nsp13 helicase, dsDNA
nsp13 helicasevariant D534A	SARS-CoV-2	ATP	0.26	0.135	1.9	pH 7.5, 37 °C	PMID 41581884	Open record: nsp13 helicase variant D534A, ATP
nsp13 helicasevariant L405D	SARS-CoV-2	ATP	0.9	0.118	10	pH 7.5, 37 °C	PMID 41581884	Open record: nsp13 helicase variant L405D, ATP
nsp13 helicasevariant L405D	SARS-CoV-2	dsDNA	10	—	3 × 10³	pH 7.5, 37 °C	PMID 41581884	Open record: nsp13 helicase variant L405D, dsDNA
nsp13 helicasevariant S535A	SARS-CoV-2	ATP100 nM labeled dsDNA	2.8	0.094	30	pH 7.5, 37 °C	PMID 41581884	Open record: nsp13 helicase variant S535A, ATP
nsp13 helicasevariant S535A	SARS-CoV-2	ATPunlabeled dsDNA present	0.47	0.176	2.7	pH 7.5, 37 °C	PMID 41581884	Open record: nsp13 helicase variant S535A, ATP
nsp13 helicasevariant S535A	SARS-CoV-2	dsDNA	2.4	1.75 × 10⁻⁴	1.6 × 10⁴	pH 7.5, 37 °C	PMID 41581884	Open record: nsp13 helicase variant S535A, dsDNA
nsp13 helicasevariant T532A	SARS-CoV-2	ATP100 nM labeled dsDNA	2.9	0.121	24	pH 7.5, 37 °C	PMID 41581884	Open record: nsp13 helicase variant T532A, ATP
nsp13 helicasevariant T532A	SARS-CoV-2	ATPunlabeled dsDNA present	0.61	0.126	5	pH 7.5, 37 °C	PMID 41581884	Open record: nsp13 helicase variant T532A, ATP
nsp13 helicasevariant T532A	SARS-CoV-2	dsDNA	3.7	2.82 × 10⁻⁴	1.4 × 10⁴	pH 7.5, 37 °C	PMID 41581884	Open record: nsp13 helicase variant T532A, dsDNA
RNA-dependent RNA polymerase (L protein)	Lassa virus	adenosine 5'-triphosphate	—	1.1 × 10⁻⁴	—	pH 8, 30 °C	PMID 32284326	Open record: RNA-dependent RNA polymerase (L protein), adenosine 5'-triphosphate
RNA-dependent RNA polymerase (L protein)	Lassa virus	remdesivir triphosphate	—	0.0013	—	pH 8, 30 °C	PMID 32284326	Open record: RNA-dependent RNA polymerase (L protein), remdesivir triphosphate
RNA-dependent RNA polymerase (nsp12/nsp8 complex)	Severe acute respiratory syndrome coronavirus	adenosine 5'-triphosphate	—	3 × 10⁻⁵	—	pH 8, 30 °C	PMID 32284326	Open record: RNA-dependent RNA polymerase (nsp12/nsp8 complex), adenosine 5'-triphosphate
RNA-dependent RNA polymerase (nsp12/nsp8 complex)	Severe acute respiratory syndrome coronavirus	remdesivir triphosphate	—	1 × 10⁻⁵	—	pH 8, 30 °C	PMID 32284326	Open record: RNA-dependent RNA polymerase (nsp12/nsp8 complex), remdesivir triphosphate
SARS-CoV-2 RNA-dependent RNA polymerase (RdRp), NSP12/NSP7/NSP8 complex	Severe acute respiratory syndrome coronavirus 2	ATP	240	0.32	740	pH 7, 37 °C	PMID 33283177	Open record: SARS-CoV-2 RNA-dependent RNA polymerase (RdRp), NSP12/NSP7/NSP8 complex, ATP
SARS-CoV-2 RNA-dependent RNA polymerase (RdRp), NSP12/NSP7/NSP8 complex	Severe acute respiratory syndrome coronavirus 2	Remdesivir 5'-triphosphatefirst RTP incorporation	68	0.053	1.29 × 10³	pH 7, 37 °C	PMID 33283177	Open record: SARS-CoV-2 RNA-dependent RNA polymerase (RdRp), NSP12/NSP7/NSP8 complex, Remdesivir 5'-triphosphate
SARS-CoV-2 RNA-dependent RNA polymerase (RdRp), NSP12/NSP7/NSP8 complex	Severe acute respiratory syndrome coronavirus 2	Remdesivir 5'-triphosphatesecond RTP incorporation	3.62	0.019	190	pH 7, 37 °C	PMID 33283177	Open record: SARS-CoV-2 RNA-dependent RNA polymerase (RdRp), NSP12/NSP7/NSP8 complex, Remdesivir 5'-triphosphate
SARS-CoV-2 RNA-dependent RNA polymerase (RdRp), NSP12/NSP7/NSP8 complex	Severe acute respiratory syndrome coronavirus 2	Remdesivir 5'-triphosphatethird RTP incorporation	3.76	0.021	180	pH 7, 37 °C	PMID 33283177	Open record: SARS-CoV-2 RNA-dependent RNA polymerase (RdRp), NSP12/NSP7/NSP8 complex, Remdesivir 5'-triphosphate
SARS-CoV-2 RNA-dependent RNA polymerase (RdRp), NSP12/NSP7/NSP8 complex	Severe acute respiratory syndrome coronavirus 2	UTPfirst UTP incorporation	308	0.13	2.3 × 10³	pH 7, 37 °C	PMID 33283177	Open record: SARS-CoV-2 RNA-dependent RNA polymerase (RdRp), NSP12/NSP7/NSP8 complex, UTP
SARS-CoV-2 RNA-dependent RNA polymerase (RdRp), NSP12/NSP7/NSP8 complex	Severe acute respiratory syndrome coronavirus 2	UTPsecond UTP incorporation	680	0.4	1.7 × 10³	pH 7, 37 °C	PMID 33283177	Open record: SARS-CoV-2 RNA-dependent RNA polymerase (RdRp), NSP12/NSP7/NSP8 complex, UTP
tobacco etch virus nuclear-inclusion-a endopeptidase (TEV protease)variant C19V/L56V/C110V/C130S/S135G/S219V (TEV Hexa)	tobacco etch virus	N-terminal fragment (residues 1-96) of dynein intermediate chain carrying an N-terminal 6His tag, a short flexible linker and the TEV protease cleavage site ENLYFQMapparent kcat; apparent Km; apparent kcat/Km	0.26	0.12	2.2	pH 7.4, 30 °C	PMID 39969093	Open record: tobacco etch virus nuclear-inclusion-a endopeptidase (TEV protease) variant C19V/L56V/C110V/C130S/S135G/S219V (TEV Hexa), N-terminal fragment (residues 1-96) of dynein intermediate chain carrying an N-terminal 6His tag, a short flexible linker and the TEV protease cleavage site ENLYFQM
tobacco etch virus nuclear-inclusion-a endopeptidase (TEV protease)variant C19V/L56V/C110V/C130S/S135G/S219V (TEV Hexa)	tobacco etch virus	N-terminal fragment (residues 1-96) of dynein intermediate chain carrying an N-terminal 6His tag, a short flexible linker and the TEV protease cleavage site ENLYFQM	0.26	0.044	6	pH 7.4, 30 °C	PMID 39969093	Open record: tobacco etch virus nuclear-inclusion-a endopeptidase (TEV protease) variant C19V/L56V/C110V/C130S/S135G/S219V (TEV Hexa), N-terminal fragment (residues 1-96) of dynein intermediate chain carrying an N-terminal 6His tag, a short flexible linker and the TEV protease cleavage site ENLYFQM
tobacco etch virus nuclear-inclusion-a endopeptidase (TEV protease)variant L56V/S135G/S219V (TEV Triple)	tobacco etch virus	N-terminal fragment (residues 1-96) of dynein intermediate chain carrying an N-terminal 6His tag, a short flexible linker and the TEV protease cleavage site ENLYFQMapparent kcat; apparent Km; apparent kcat/Km	0.27	0.08	3.3	pH 7.4, 30 °C	PMID 39969093	Open record: tobacco etch virus nuclear-inclusion-a endopeptidase (TEV protease) variant L56V/S135G/S219V (TEV Triple), N-terminal fragment (residues 1-96) of dynein intermediate chain carrying an N-terminal 6His tag, a short flexible linker and the TEV protease cleavage site ENLYFQM
tobacco etch virus nuclear-inclusion-a endopeptidase (TEV protease)variant L56V/S135G/S219V (TEV Triple)	tobacco etch virus	N-terminal fragment (residues 1-96) of dynein intermediate chain carrying an N-terminal 6His tag, a short flexible linker and the TEV protease cleavage site ENLYFQM	0.26	0.045	6	pH 7.4, 30 °C	PMID 39969093	Open record: tobacco etch virus nuclear-inclusion-a endopeptidase (TEV protease) variant L56V/S135G/S219V (TEV Triple), N-terminal fragment (residues 1-96) of dynein intermediate chain carrying an N-terminal 6His tag, a short flexible linker and the TEV protease cleavage site ENLYFQM
tobacco etch virus nuclear-inclusion-a endopeptidase (TEV protease)variant S219V	tobacco etch virus	N-terminal fragment (residues 1-96) of dynein intermediate chain carrying an N-terminal 6His tag, a short flexible linker and the TEV protease cleavage site ENLYFQMapparent kcat; apparent Km; apparent kcat/Km	0.28	0.22	1.3	pH 7.4, 30 °C	PMID 39969093	Open record: tobacco etch virus nuclear-inclusion-a endopeptidase (TEV protease) variant S219V, N-terminal fragment (residues 1-96) of dynein intermediate chain carrying an N-terminal 6His tag, a short flexible linker and the TEV protease cleavage site ENLYFQM
tobacco etch virus nuclear-inclusion-a endopeptidase (TEV protease)variant S219V	tobacco etch virus	N-terminal fragment (residues 1-96) of dynein intermediate chain carrying an N-terminal 6His tag, a short flexible linker and the TEV protease cleavage site ENLYFQM	0.27	0.11	2.6	pH 7.4, 30 °C	PMID 39969093	Open record: tobacco etch virus nuclear-inclusion-a endopeptidase (TEV protease) variant S219V, N-terminal fragment (residues 1-96) of dynein intermediate chain carrying an N-terminal 6His tag, a short flexible linker and the TEV protease cleavage site ENLYFQM
ΔNS2B-NS3pro (NS2B/NS3 protease complex)	Dengue virus type 1	t-butyloxycarbonyl-glycyl-L-arginyl-L-arginine 4-methylcoumaryl-7-amide	0.101	0.505	0.2	pH 9, 37 °C	PMID 16504332	Open record: ΔNS2B-NS3pro (NS2B/NS3 protease complex), t-butyloxycarbonyl-glycyl-L-arginyl-L-arginine 4-methylcoumaryl-7-amide
ΔNS2B-NS3pro (NS2B/NS3 protease complex)	Dengue virus type 1	t-butyloxycarbonyl-glycyl-L-lysyl-L-arginine 4-methylcoumaryl-7-amide	0.153	3.74	0.041	pH 9, 37 °C	PMID 16504332	Open record: ΔNS2B-NS3pro (NS2B/NS3 protease complex), t-butyloxycarbonyl-glycyl-L-lysyl-L-arginine 4-methylcoumaryl-7-amide
ΔNS2B-NS3pro (NS2B/NS3 protease complex)	Dengue virus type 3	t-butyloxycarbonyl-glycyl-L-arginyl-L-arginine 4-methylcoumaryl-7-amide	0.163	2.7	0.06	pH 9, 37 °C	PMID 16504332	Open record: ΔNS2B-NS3pro (NS2B/NS3 protease complex), t-butyloxycarbonyl-glycyl-L-arginyl-L-arginine 4-methylcoumaryl-7-amide
ΔNS2B-NS3pro (NS2B/NS3 protease complex)	Dengue virus type 3	t-butyloxycarbonyl-glycyl-L-lysyl-L-arginine 4-methylcoumaryl-7-amide	0.007	0.181	0.034	pH 9, 37 °C	PMID 16504332	Open record: ΔNS2B-NS3pro (NS2B/NS3 protease complex), t-butyloxycarbonyl-glycyl-L-lysyl-L-arginine 4-methylcoumaryl-7-amide
ΔNS2B-NS3pro (NS2B/NS3 protease complex)	Langat virus	t-butyloxycarbonyl-glycyl-L-arginyl-L-arginine 4-methylcoumaryl-7-amide	0.007	0.324	0.02	pH 9, 37 °C	PMID 16504332	Open record: ΔNS2B-NS3pro (NS2B/NS3 protease complex), t-butyloxycarbonyl-glycyl-L-arginyl-L-arginine 4-methylcoumaryl-7-amide