Values CatLog read from this paper
An improved variant of tobacco etch virus (TEV) protease that does not need reducing agents
PMID 39969093doi:10.1002/pro.70049Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 6 records (18 values) from this paper: tobacco etch virus nuclear-inclusion-a endopeptidase (TEV protease) from tobacco etch virus.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
EC 2.7.7.48, EC 3.4.22.44, EC 3.4.22.45
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| tobacco etch virus nuclear-inclusion-a endopeptidase (TEV protease)variant C19V/L56V/C110V/C130S/S135G/S219V (TEV Hexa) | tobacco etch virus | N-terminal fragment (residues 1-96) of dynein intermediate chain carrying an N-terminal 6His tag, a short flexible linker and the TEV protease cleavage site ENLYFQMapparent kcat; apparent Km; apparent kcat/Km | 0.26 | 0.12 | 2.2 | pH 7.4, 30 °C | Open record: tobacco etch virus nuclear-inclusion-a endopeptidase (TEV protease) variant C19V/L56V/C110V/C130S/S135G/S219V (TEV Hexa), N-terminal fragment (residues 1-96) of dynein intermediate chain carrying an N-terminal 6His tag, a short flexible linker and the TEV protease cleavage site ENLYFQM |
| tobacco etch virus nuclear-inclusion-a endopeptidase (TEV protease)variant C19V/L56V/C110V/C130S/S135G/S219V (TEV Hexa) | tobacco etch virus | N-terminal fragment (residues 1-96) of dynein intermediate chain carrying an N-terminal 6His tag, a short flexible linker and the TEV protease cleavage site ENLYFQM | 0.26 | 0.044 | 6 | pH 7.4, 30 °C | Open record: tobacco etch virus nuclear-inclusion-a endopeptidase (TEV protease) variant C19V/L56V/C110V/C130S/S135G/S219V (TEV Hexa), N-terminal fragment (residues 1-96) of dynein intermediate chain carrying an N-terminal 6His tag, a short flexible linker and the TEV protease cleavage site ENLYFQM |
| tobacco etch virus nuclear-inclusion-a endopeptidase (TEV protease)variant L56V/S135G/S219V (TEV Triple) | tobacco etch virus | N-terminal fragment (residues 1-96) of dynein intermediate chain carrying an N-terminal 6His tag, a short flexible linker and the TEV protease cleavage site ENLYFQMapparent kcat; apparent Km; apparent kcat/Km | 0.27 | 0.08 | 3.3 | pH 7.4, 30 °C | Open record: tobacco etch virus nuclear-inclusion-a endopeptidase (TEV protease) variant L56V/S135G/S219V (TEV Triple), N-terminal fragment (residues 1-96) of dynein intermediate chain carrying an N-terminal 6His tag, a short flexible linker and the TEV protease cleavage site ENLYFQM |
| tobacco etch virus nuclear-inclusion-a endopeptidase (TEV protease)variant L56V/S135G/S219V (TEV Triple) | tobacco etch virus | N-terminal fragment (residues 1-96) of dynein intermediate chain carrying an N-terminal 6His tag, a short flexible linker and the TEV protease cleavage site ENLYFQM | 0.26 | 0.045 | 6 | pH 7.4, 30 °C | Open record: tobacco etch virus nuclear-inclusion-a endopeptidase (TEV protease) variant L56V/S135G/S219V (TEV Triple), N-terminal fragment (residues 1-96) of dynein intermediate chain carrying an N-terminal 6His tag, a short flexible linker and the TEV protease cleavage site ENLYFQM |
| tobacco etch virus nuclear-inclusion-a endopeptidase (TEV protease)variant S219V | tobacco etch virus | N-terminal fragment (residues 1-96) of dynein intermediate chain carrying an N-terminal 6His tag, a short flexible linker and the TEV protease cleavage site ENLYFQMapparent kcat; apparent Km; apparent kcat/Km | 0.28 | 0.22 | 1.3 | pH 7.4, 30 °C | Open record: tobacco etch virus nuclear-inclusion-a endopeptidase (TEV protease) variant S219V, N-terminal fragment (residues 1-96) of dynein intermediate chain carrying an N-terminal 6His tag, a short flexible linker and the TEV protease cleavage site ENLYFQM |
| tobacco etch virus nuclear-inclusion-a endopeptidase (TEV protease)variant S219V | tobacco etch virus | N-terminal fragment (residues 1-96) of dynein intermediate chain carrying an N-terminal 6His tag, a short flexible linker and the TEV protease cleavage site ENLYFQM | 0.27 | 0.11 | 2.6 | pH 7.4, 30 °C | Open record: tobacco etch virus nuclear-inclusion-a endopeptidase (TEV protease) variant S219V, N-terminal fragment (residues 1-96) of dynein intermediate chain carrying an N-terminal 6His tag, a short flexible linker and the TEV protease cleavage site ENLYFQM |