EC 3.4.22.44: nuclear-inclusion-a endopeptidase

HydrolasesOpen in Browse

CatLog read 6 records for EC 3.4.22.44 from 1 paper, for 1 organism. Names in these papers: tobacco etch virus nuclear-inclusion-a endopeptidase (TEV protease).

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units.

CatLog also holds 17 records for EC 3.4.22.44 that are not yet re-read: from its earlier version, built from the enzyme databases and partly checked against the papers. All records for EC 3.4.22.44 in Browse.

Papers

Papers with records for EC 3.4.22.44
PaperOrganismsRecords
An improved variant of tobacco etch virus (TEV) protease that does not need reducing agentstobacco etch virus6

Records

Records for EC 3.4.22.44 read from the paper
EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)ConditionsPaperOpen record
tobacco etch virus nuclear-inclusion-a endopeptidase (TEV protease)variant C19V/L56V/C110V/C130S/S135G/S219V (TEV Hexa)tobacco etch virusN-terminal fragment (residues 1-96) of dynein intermediate chain carrying an N-terminal 6His tag, a short flexible linker and the TEV protease cleavage site ENLYFQMapparent kcat; apparent Km; apparent kcat/Km0.260.122.2pH 7.4, 30 °CPMID 39969093Open record: tobacco etch virus nuclear-inclusion-a endopeptidase (TEV protease) variant C19V/L56V/C110V/C130S/S135G/S219V (TEV Hexa), N-terminal fragment (residues 1-96) of dynein intermediate chain carrying an N-terminal 6His tag, a short flexible linker and the TEV protease cleavage site ENLYFQM
tobacco etch virus nuclear-inclusion-a endopeptidase (TEV protease)variant C19V/L56V/C110V/C130S/S135G/S219V (TEV Hexa)tobacco etch virusN-terminal fragment (residues 1-96) of dynein intermediate chain carrying an N-terminal 6His tag, a short flexible linker and the TEV protease cleavage site ENLYFQM0.260.0446pH 7.4, 30 °CPMID 39969093Open record: tobacco etch virus nuclear-inclusion-a endopeptidase (TEV protease) variant C19V/L56V/C110V/C130S/S135G/S219V (TEV Hexa), N-terminal fragment (residues 1-96) of dynein intermediate chain carrying an N-terminal 6His tag, a short flexible linker and the TEV protease cleavage site ENLYFQM
tobacco etch virus nuclear-inclusion-a endopeptidase (TEV protease)variant L56V/S135G/S219V (TEV Triple)tobacco etch virusN-terminal fragment (residues 1-96) of dynein intermediate chain carrying an N-terminal 6His tag, a short flexible linker and the TEV protease cleavage site ENLYFQMapparent kcat; apparent Km; apparent kcat/Km0.270.083.3pH 7.4, 30 °CPMID 39969093Open record: tobacco etch virus nuclear-inclusion-a endopeptidase (TEV protease) variant L56V/S135G/S219V (TEV Triple), N-terminal fragment (residues 1-96) of dynein intermediate chain carrying an N-terminal 6His tag, a short flexible linker and the TEV protease cleavage site ENLYFQM
tobacco etch virus nuclear-inclusion-a endopeptidase (TEV protease)variant L56V/S135G/S219V (TEV Triple)tobacco etch virusN-terminal fragment (residues 1-96) of dynein intermediate chain carrying an N-terminal 6His tag, a short flexible linker and the TEV protease cleavage site ENLYFQM0.260.0456pH 7.4, 30 °CPMID 39969093Open record: tobacco etch virus nuclear-inclusion-a endopeptidase (TEV protease) variant L56V/S135G/S219V (TEV Triple), N-terminal fragment (residues 1-96) of dynein intermediate chain carrying an N-terminal 6His tag, a short flexible linker and the TEV protease cleavage site ENLYFQM
tobacco etch virus nuclear-inclusion-a endopeptidase (TEV protease)variant S219Vtobacco etch virusN-terminal fragment (residues 1-96) of dynein intermediate chain carrying an N-terminal 6His tag, a short flexible linker and the TEV protease cleavage site ENLYFQMapparent kcat; apparent Km; apparent kcat/Km0.280.221.3pH 7.4, 30 °CPMID 39969093Open record: tobacco etch virus nuclear-inclusion-a endopeptidase (TEV protease) variant S219V, N-terminal fragment (residues 1-96) of dynein intermediate chain carrying an N-terminal 6His tag, a short flexible linker and the TEV protease cleavage site ENLYFQM
tobacco etch virus nuclear-inclusion-a endopeptidase (TEV protease)variant S219Vtobacco etch virusN-terminal fragment (residues 1-96) of dynein intermediate chain carrying an N-terminal 6His tag, a short flexible linker and the TEV protease cleavage site ENLYFQM0.270.112.6pH 7.4, 30 °CPMID 39969093Open record: tobacco etch virus nuclear-inclusion-a endopeptidase (TEV protease) variant S219V, N-terminal fragment (residues 1-96) of dynein intermediate chain carrying an N-terminal 6His tag, a short flexible linker and the TEV protease cleavage site ENLYFQM