Values CatLog read from this paper
Cysteine Disulfide Traps Reveal Distinct Conformational Ensembles in Dengue Virus NS2B-NS3 Protease
PMID 30472839doi:10.1021/acs.biochem.8b00978Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 13 records (39 values) from this paper: NS2B-NS3 protease (NS2B-NS3pro) from Dengue virus serotype 2.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| NS2B-NS3 protease (NS2B-NS3pro) | Dengue virus serotype 2 | N-acetyl-Gly-Arg-Arg-7-amino-4-methylcoumarinN-terminally His6-tagged single chain | 0.05 | 0.07 | 0.7 | pH 8.5, 37 °C | Open record: NS2B-NS3 protease (NS2B-NS3pro), N-acetyl-Gly-Arg-Arg-7-amino-4-methylcoumarin |
| NS2B-NS3 protease (NS2B-NS3pro) | Dengue virus serotype 2 | N-acetyl-Gly-Arg-Arg-7-amino-4-methylcoumarin | 0.09 | 0.04 | 2 | pH 8.5, 37 °C | Open record: NS2B-NS3 protease (NS2B-NS3pro), N-acetyl-Gly-Arg-Arg-7-amino-4-methylcoumarin |
| NS2B-NS3 protease (NS2B-NS3pro)variant A125C/V162C | Dengue virus serotype 2 | N-acetyl-Gly-Arg-Arg-7-amino-4-methylcoumarinN-terminally His6-tagged single chain | 0.02 | 0.03 | 0.8 | pH 8.5, 37 °C | Open record: NS2B-NS3 protease (NS2B-NS3pro) variant A125C/V162C, N-acetyl-Gly-Arg-Arg-7-amino-4-methylcoumarin |
| NS2B-NS3 protease (NS2B-NS3pro)variant A125C/V162C | Dengue virus serotype 2 | N-acetyl-Gly-Arg-Arg-7-amino-4-methylcoumarin | 0.01 | 0.05 | 0.3 | pH 8.5, 37 °C | Open record: NS2B-NS3 protease (NS2B-NS3pro) variant A125C/V162C, N-acetyl-Gly-Arg-Arg-7-amino-4-methylcoumarin |
| NS2B-NS3 protease (NS2B-NS3pro)variant I73C*/P106C | Dengue virus serotype 2 | N-acetyl-Gly-Arg-Arg-7-amino-4-methylcoumarinN-terminally His6-tagged single chain | 0.03 | 0.04 | 0.8 | pH 8.5, 37 °C | Open record: NS2B-NS3 protease (NS2B-NS3pro) variant I73C*/P106C, N-acetyl-Gly-Arg-Arg-7-amino-4-methylcoumarin |
| NS2B-NS3 protease (NS2B-NS3pro)variant I73C*/P106C | Dengue virus serotype 2 | N-acetyl-Gly-Arg-Arg-7-amino-4-methylcoumarin | 0.08 | 0.05 | 2 | pH 8.5, 37 °C | Open record: NS2B-NS3 protease (NS2B-NS3pro) variant I73C*/P106C, N-acetyl-Gly-Arg-Arg-7-amino-4-methylcoumarin |
| NS2B-NS3 protease (NS2B-NS3pro)variant K117A | Dengue virus serotype 2 | N-acetyl-Gly-Arg-Arg-7-amino-4-methylcoumarinN-terminally His6-tagged single chain | 0.006 | 0.04 | 0.2 | pH 8.5, 37 °C | Open record: NS2B-NS3 protease (NS2B-NS3pro) variant K117A, N-acetyl-Gly-Arg-Arg-7-amino-4-methylcoumarin |
| NS2B-NS3 protease (NS2B-NS3pro)variant K117A | Dengue virus serotype 2 | N-acetyl-Gly-Arg-Arg-7-amino-4-methylcoumarin | 0.05 | 0.08 | 0.7 | pH 8.5, 37 °C | Open record: NS2B-NS3 protease (NS2B-NS3pro) variant K117A, N-acetyl-Gly-Arg-Arg-7-amino-4-methylcoumarin |
| NS2B-NS3 protease (NS2B-NS3pro)variant K117C | Dengue virus serotype 2 | N-acetyl-Gly-Arg-Arg-7-amino-4-methylcoumarin | 0.02 | 0.05 | 0.44 | pH 8.5, 37 °C | Open record: NS2B-NS3 protease (NS2B-NS3pro) variant K117C, N-acetyl-Gly-Arg-Arg-7-amino-4-methylcoumarin |
| NS2B-NS3 protease (NS2B-NS3pro)variant K117R | Dengue virus serotype 2 | N-acetyl-Gly-Arg-Arg-7-amino-4-methylcoumarin | 0.04 | 0.05 | 0.8 | pH 8.5, 37 °C | Open record: NS2B-NS3 protease (NS2B-NS3pro) variant K117R, N-acetyl-Gly-Arg-Arg-7-amino-4-methylcoumarin |
| NS2B-NS3 protease (NS2B-NS3pro)variant S75C* | Dengue virus serotype 2 | N-acetyl-Gly-Arg-Arg-7-amino-4-methylcoumarin | 0.05 | 0.04 | 1 | pH 8.5, 37 °C | Open record: NS2B-NS3 protease (NS2B-NS3pro) variant S75C*, N-acetyl-Gly-Arg-Arg-7-amino-4-methylcoumarin |
| NS2B-NS3 protease (NS2B-NS3pro)variant S75C*/K117C | Dengue virus serotype 2 | N-acetyl-Gly-Arg-Arg-7-amino-4-methylcoumarinN-terminally His6-tagged single chain | 0.02 | 0.03 | 0.8 | pH 8.5, 37 °C | Open record: NS2B-NS3 protease (NS2B-NS3pro) variant S75C*/K117C, N-acetyl-Gly-Arg-Arg-7-amino-4-methylcoumarin |
| NS2B-NS3 protease (NS2B-NS3pro)variant S75C*/K117C | Dengue virus serotype 2 | N-acetyl-Gly-Arg-Arg-7-amino-4-methylcoumarin | 0.07 | 0.04 | 2 | pH 8.5, 37 °C | Open record: NS2B-NS3 protease (NS2B-NS3pro) variant S75C*/K117C, N-acetyl-Gly-Arg-Arg-7-amino-4-methylcoumarin |