Values CatLog read from this paper
Motif V is an allosteric couple between the SARS-CoV-2 nsp13 nucleotide triphosphatase and helicase active sites
PMID 41581884doi:10.1016/j.jbc.2026.111198Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 12 records (35 values) from this paper: nsp13 helicase from SARS-CoV-2.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
EC 2.1.1.56, EC 2.1.1.57, EC 2.7.7.48, EC 2.7.7.50, EC 3.4.19.12, EC 3.4.22.69, EC 3.6.4.12, EC 3.6.4.13
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| nsp13 helicase | SARS-CoV-2 | ATP100 nM labeled dsDNA | 1.4 | 0.122 | 13 | pH 7.5, 37 °C | Open record: nsp13 helicase, ATP |
| nsp13 helicase | SARS-CoV-2 | ATPunlabeled dsDNA present | 0.37 | 0.156 | 2.7 | pH 7.5, 37 °C | Open record: nsp13 helicase, ATP |
| nsp13 helicase | SARS-CoV-2 | dsDNA | 1.5 | 2.85 × 10⁻⁴ | 6 × 10³ | pH 7.5, 37 °C | Open record: nsp13 helicase, dsDNA |
| nsp13 helicasevariant D534A | SARS-CoV-2 | ATP | 0.26 | 0.135 | 1.9 | pH 7.5, 37 °C | Open record: nsp13 helicase variant D534A, ATP |
| nsp13 helicasevariant L405D | SARS-CoV-2 | ATP | 0.9 | 0.118 | 10 | pH 7.5, 37 °C | Open record: nsp13 helicase variant L405D, ATP |
| nsp13 helicasevariant L405D | SARS-CoV-2 | dsDNA | 10 | — | 3 × 10³ | pH 7.5, 37 °C | Open record: nsp13 helicase variant L405D, dsDNA |
| nsp13 helicasevariant S535A | SARS-CoV-2 | ATP100 nM labeled dsDNA | 2.8 | 0.094 | 30 | pH 7.5, 37 °C | Open record: nsp13 helicase variant S535A, ATP |
| nsp13 helicasevariant S535A | SARS-CoV-2 | ATPunlabeled dsDNA present | 0.47 | 0.176 | 2.7 | pH 7.5, 37 °C | Open record: nsp13 helicase variant S535A, ATP |
| nsp13 helicasevariant S535A | SARS-CoV-2 | dsDNA | 2.4 | 1.75 × 10⁻⁴ | 1.6 × 10⁴ | pH 7.5, 37 °C | Open record: nsp13 helicase variant S535A, dsDNA |
| nsp13 helicasevariant T532A | SARS-CoV-2 | ATP100 nM labeled dsDNA | 2.9 | 0.121 | 24 | pH 7.5, 37 °C | Open record: nsp13 helicase variant T532A, ATP |
| nsp13 helicasevariant T532A | SARS-CoV-2 | ATPunlabeled dsDNA present | 0.61 | 0.126 | 5 | pH 7.5, 37 °C | Open record: nsp13 helicase variant T532A, ATP |
| nsp13 helicasevariant T532A | SARS-CoV-2 | dsDNA | 3.7 | 2.82 × 10⁻⁴ | 1.4 × 10⁴ | pH 7.5, 37 °C | Open record: nsp13 helicase variant T532A, dsDNA |