EC 2.7.7.50: mRNA guanylyltransferase
TransferasesOpen in Browse
CatLog read 59 records for EC 2.7.7.50 from 5 papers, for 5 organisms. Names in these papers: SARS-CoV main protease (3C-like protease), TbCe1 (cytoplasmic capping enzyme, RNA kinase activity), nsp13 helicase, SARS-CoV main protease (Mpro) and 1 more.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units.
CatLog also holds 11 records for EC 2.7.7.50 that are not yet re-read: from its earlier version, built from the enzyme databases and partly checked against the papers. All records for EC 2.7.7.50 in Browse.
Papers
| Paper | Organisms | Records |
|---|---|---|
| Correlation between dissociation and catalysis of SARS-CoV main protease | severe acute respiratory syndrome coronavirus (SARS-CoV) | 24 |
| Trypanosome mRNA recapping is triggered by hypermethylation originating from cap 4 | Trypanosoma brucei | 14 |
| Motif V is an allosteric couple between the SARS-CoV-2 nsp13 nucleotide triphosphatase and helicase active sites | SARS-CoV-2 | 12 |
| Mutation of Glu-166 blocks the substrate-induced dimerization of SARS coronavirus main protease | SARS-CoV | 7 |
| Remdesivir is a direct-acting antiviral that inhibits RNA-dependent RNA polymerase from severe acute respiratory syndrome coronavirus 2 with high potency | Severe acute respiratory syndrome coronavirus | 2 |
Records
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Paper | Open record |
|---|---|---|---|---|---|---|---|---|
| nsp13 helicase | SARS-CoV-2 | ATP100 nM labeled dsDNA | 1.4 | 0.122 | 13 | pH 7.5, 37 °C | PMID 41581884 | Open record: nsp13 helicase, ATP |
| nsp13 helicase | SARS-CoV-2 | ATPunlabeled dsDNA present | 0.37 | 0.156 | 2.7 | pH 7.5, 37 °C | PMID 41581884 | Open record: nsp13 helicase, ATP |
| nsp13 helicase | SARS-CoV-2 | dsDNA | 1.5 | 2.85 × 10⁻⁴ | 6 × 10³ | pH 7.5, 37 °C | PMID 41581884 | Open record: nsp13 helicase, dsDNA |
| nsp13 helicasevariant D534A | SARS-CoV-2 | ATP | 0.26 | 0.135 | 1.9 | pH 7.5, 37 °C | PMID 41581884 | Open record: nsp13 helicase variant D534A, ATP |
| nsp13 helicasevariant L405D | SARS-CoV-2 | ATP | 0.9 | 0.118 | 10 | pH 7.5, 37 °C | PMID 41581884 | Open record: nsp13 helicase variant L405D, ATP |
| nsp13 helicasevariant L405D | SARS-CoV-2 | dsDNA | 10 | — | 3 × 10³ | pH 7.5, 37 °C | PMID 41581884 | Open record: nsp13 helicase variant L405D, dsDNA |
| nsp13 helicasevariant S535A | SARS-CoV-2 | ATP100 nM labeled dsDNA | 2.8 | 0.094 | 30 | pH 7.5, 37 °C | PMID 41581884 | Open record: nsp13 helicase variant S535A, ATP |
| nsp13 helicasevariant S535A | SARS-CoV-2 | ATPunlabeled dsDNA present | 0.47 | 0.176 | 2.7 | pH 7.5, 37 °C | PMID 41581884 | Open record: nsp13 helicase variant S535A, ATP |
| nsp13 helicasevariant S535A | SARS-CoV-2 | dsDNA | 2.4 | 1.75 × 10⁻⁴ | 1.6 × 10⁴ | pH 7.5, 37 °C | PMID 41581884 | Open record: nsp13 helicase variant S535A, dsDNA |
| nsp13 helicasevariant T532A | SARS-CoV-2 | ATP100 nM labeled dsDNA | 2.9 | 0.121 | 24 | pH 7.5, 37 °C | PMID 41581884 | Open record: nsp13 helicase variant T532A, ATP |
| nsp13 helicasevariant T532A | SARS-CoV-2 | ATPunlabeled dsDNA present | 0.61 | 0.126 | 5 | pH 7.5, 37 °C | PMID 41581884 | Open record: nsp13 helicase variant T532A, ATP |
| nsp13 helicasevariant T532A | SARS-CoV-2 | dsDNA | 3.7 | 2.82 × 10⁻⁴ | 1.4 × 10⁴ | pH 7.5, 37 °C | PMID 41581884 | Open record: nsp13 helicase variant T532A, dsDNA |
| RNA-dependent RNA polymerase (nsp12/nsp8 complex) | Severe acute respiratory syndrome coronavirus | adenosine 5'-triphosphate | — | 3 × 10⁻⁵ | — | pH 8, 30 °C | PMID 32284326 | Open record: RNA-dependent RNA polymerase (nsp12/nsp8 complex), adenosine 5'-triphosphate |
| RNA-dependent RNA polymerase (nsp12/nsp8 complex) | Severe acute respiratory syndrome coronavirus | remdesivir triphosphate | — | 1 × 10⁻⁵ | — | pH 8, 30 °C | PMID 32284326 | Open record: RNA-dependent RNA polymerase (nsp12/nsp8 complex), remdesivir triphosphate |
| SARS-CoV main protease (3C-like protease) | severe acute respiratory syndrome coronavirus (SARS-CoV) | ortho-aminobenzoic acid (ABZ)-TSAVLQSGFRK-2,4-dinitrophenyl amide (DNP); 11-mer peptide cleaved at the Q-S site | 0.032 | 0.011 | 2.9 calc. | pH 7.6, 30 °C | PMID 18275836 | Open record: SARS-CoV main protease (3C-like protease), ortho-aminobenzoic acid (ABZ)-TSAVLQSGFRK-2,4-dinitrophenyl amide (DNP); 11-mer peptide cleaved at the Q-S site |
| SARS-CoV main protease (3C-like protease)variant Q299A | severe acute respiratory syndrome coronavirus (SARS-CoV) | ortho-aminobenzoic acid (ABZ)-TSAVLQSGFRK-2,4-dinitrophenyl amide (DNP); 11-mer peptide cleaved at the Q-S site | 7 × 10⁻⁴ | 0.018 | 0.039 calc. | pH 7.6, 30 °C | PMID 18275836 | Open record: SARS-CoV main protease (3C-like protease) variant Q299A, ortho-aminobenzoic acid (ABZ)-TSAVLQSGFRK-2,4-dinitrophenyl amide (DNP); 11-mer peptide cleaved at the Q-S site |
| SARS-CoV main protease (3C-like protease)variant Q299E | severe acute respiratory syndrome coronavirus (SARS-CoV) | ortho-aminobenzoic acid (ABZ)-TSAVLQSGFRK-2,4-dinitrophenyl amide (DNP); 11-mer peptide cleaved at the Q-S site | 0.0022 | 0.005 | 0.44 calc. | pH 7.6, 30 °C | PMID 18275836 | Open record: SARS-CoV main protease (3C-like protease) variant Q299E, ortho-aminobenzoic acid (ABZ)-TSAVLQSGFRK-2,4-dinitrophenyl amide (DNP); 11-mer peptide cleaved at the Q-S site |
| SARS-CoV main protease (3C-like protease)variant Q299K | severe acute respiratory syndrome coronavirus (SARS-CoV) | ortho-aminobenzoic acid (ABZ)-TSAVLQSGFRK-2,4-dinitrophenyl amide (DNP); 11-mer peptide cleaved at the Q-S site | 0.0021 | 0.037 | 0.057 calc. | pH 7.6, 30 °C | PMID 18275836 | Open record: SARS-CoV main protease (3C-like protease) variant Q299K, ortho-aminobenzoic acid (ABZ)-TSAVLQSGFRK-2,4-dinitrophenyl amide (DNP); 11-mer peptide cleaved at the Q-S site |
| SARS-CoV main protease (3C-like protease)variant Q299N | severe acute respiratory syndrome coronavirus (SARS-CoV) | ortho-aminobenzoic acid (ABZ)-TSAVLQSGFRK-2,4-dinitrophenyl amide (DNP); 11-mer peptide cleaved at the Q-S site | 0.0017 | 0.005 | 0.34 calc. | pH 7.6, 30 °C | PMID 18275836 | Open record: SARS-CoV main protease (3C-like protease) variant Q299N, ortho-aminobenzoic acid (ABZ)-TSAVLQSGFRK-2,4-dinitrophenyl amide (DNP); 11-mer peptide cleaved at the Q-S site |
| SARS-CoV main protease (3C-like protease)variant R298A | severe acute respiratory syndrome coronavirus (SARS-CoV) | ortho-aminobenzoic acid (ABZ)-TSAVLQSGFRK-2,4-dinitrophenyl amide (DNP); 11-mer peptide cleaved at the Q-S site | 0.0034 | 0.011 | 0.31 calc. | pH 7.6, 30 °C | PMID 18275836 | Open record: SARS-CoV main protease (3C-like protease) variant R298A, ortho-aminobenzoic acid (ABZ)-TSAVLQSGFRK-2,4-dinitrophenyl amide (DNP); 11-mer peptide cleaved at the Q-S site |
| SARS-CoV main protease (3C-like protease)variant R298A/Q299A | severe acute respiratory syndrome coronavirus (SARS-CoV) | ortho-aminobenzoic acid (ABZ)-TSAVLQSGFRK-2,4-dinitrophenyl amide (DNP); 11-mer peptide cleaved at the Q-S site | 2.1 × 10⁻⁴ | 0.01 | 0.021 calc. | pH 7.6, 30 °C | PMID 18275836 | Open record: SARS-CoV main protease (3C-like protease) variant R298A/Q299A, ortho-aminobenzoic acid (ABZ)-TSAVLQSGFRK-2,4-dinitrophenyl amide (DNP); 11-mer peptide cleaved at the Q-S site |
| SARS-CoV main protease (3C-like protease)variant R298K | severe acute respiratory syndrome coronavirus (SARS-CoV) | ortho-aminobenzoic acid (ABZ)-TSAVLQSGFRK-2,4-dinitrophenyl amide (DNP); 11-mer peptide cleaved at the Q-S site | 0.017 | 0.016 | 1.1 calc. | pH 7.6, 30 °C | PMID 18275836 | Open record: SARS-CoV main protease (3C-like protease) variant R298K, ortho-aminobenzoic acid (ABZ)-TSAVLQSGFRK-2,4-dinitrophenyl amide (DNP); 11-mer peptide cleaved at the Q-S site |
| SARS-CoV main protease (3C-like protease)variant R298L | severe acute respiratory syndrome coronavirus (SARS-CoV) | ortho-aminobenzoic acid (ABZ)-TSAVLQSGFRK-2,4-dinitrophenyl amide (DNP); 11-mer peptide cleaved at the Q-S site | 0.0048 | 0.013 | 0.37 calc. | pH 7.6, 30 °C | PMID 18275836 | Open record: SARS-CoV main protease (3C-like protease) variant R298L, ortho-aminobenzoic acid (ABZ)-TSAVLQSGFRK-2,4-dinitrophenyl amide (DNP); 11-mer peptide cleaved at the Q-S site |
| SARS-CoV main protease (3C-like protease)variant S123A | severe acute respiratory syndrome coronavirus (SARS-CoV) | ortho-aminobenzoic acid (ABZ)-TSAVLQSGFRK-2,4-dinitrophenyl amide (DNP); 11-mer peptide cleaved at the Q-S site | 0.033 | 0.013 | 2.5 calc. | pH 7.6, 30 °C | PMID 18275836 | Open record: SARS-CoV main protease (3C-like protease) variant S123A, ortho-aminobenzoic acid (ABZ)-TSAVLQSGFRK-2,4-dinitrophenyl amide (DNP); 11-mer peptide cleaved at the Q-S site |
| SARS-CoV main protease (3C-like protease)variant S123A/R298A | severe acute respiratory syndrome coronavirus (SARS-CoV) | ortho-aminobenzoic acid (ABZ)-TSAVLQSGFRK-2,4-dinitrophenyl amide (DNP); 11-mer peptide cleaved at the Q-S site | 0.0069 | 0.012 | 0.57 calc. | pH 7.6, 30 °C | PMID 18275836 | Open record: SARS-CoV main protease (3C-like protease) variant S123A/R298A, ortho-aminobenzoic acid (ABZ)-TSAVLQSGFRK-2,4-dinitrophenyl amide (DNP); 11-mer peptide cleaved at the Q-S site |
| SARS-CoV main protease (3C-like protease)variant S123C | severe acute respiratory syndrome coronavirus (SARS-CoV) | ortho-aminobenzoic acid (ABZ)-TSAVLQSGFRK-2,4-dinitrophenyl amide (DNP); 11-mer peptide cleaved at the Q-S site | 0.035 | 0.013 | 2.7 calc. | pH 7.6, 30 °C | PMID 18275836 | Open record: SARS-CoV main protease (3C-like protease) variant S123C, ortho-aminobenzoic acid (ABZ)-TSAVLQSGFRK-2,4-dinitrophenyl amide (DNP); 11-mer peptide cleaved at the Q-S site |
| SARS-CoV main protease (3C-like protease)variant S139A | severe acute respiratory syndrome coronavirus (SARS-CoV) | ortho-aminobenzoic acid (ABZ)-TSAVLQSGFRK-2,4-dinitrophenyl amide (DNP); 11-mer peptide cleaved at the Q-S site | 0.033 | 0.01 | 3.3 calc. | pH 7.6, 30 °C | PMID 18275836 | Open record: SARS-CoV main protease (3C-like protease) variant S139A, ortho-aminobenzoic acid (ABZ)-TSAVLQSGFRK-2,4-dinitrophenyl amide (DNP); 11-mer peptide cleaved at the Q-S site |
| SARS-CoV main protease (3C-like protease)variant S139A/Q299A | severe acute respiratory syndrome coronavirus (SARS-CoV) | ortho-aminobenzoic acid (ABZ)-TSAVLQSGFRK-2,4-dinitrophenyl amide (DNP); 11-mer peptide cleaved at the Q-S site | 0.0048 | 0.021 | 0.23 calc. | pH 7.6, 30 °C | PMID 18275836 | Open record: SARS-CoV main protease (3C-like protease) variant S139A/Q299A, ortho-aminobenzoic acid (ABZ)-TSAVLQSGFRK-2,4-dinitrophenyl amide (DNP); 11-mer peptide cleaved at the Q-S site |
| SARS-CoV main protease (3C-like protease)variant Δ(297–306) | severe acute respiratory syndrome coronavirus (SARS-CoV) | ortho-aminobenzoic acid (ABZ)-TSAVLQSGFRK-2,4-dinitrophenyl amide (DNP); 11-mer peptide cleaved at the Q-S site | 4 × 10⁻⁵ | 0.021 | 0.0019 calc. | pH 7.6, 30 °C | PMID 18275836 | Open record: SARS-CoV main protease (3C-like protease) variant Δ(297–306), ortho-aminobenzoic acid (ABZ)-TSAVLQSGFRK-2,4-dinitrophenyl amide (DNP); 11-mer peptide cleaved at the Q-S site |
| SARS-CoV main protease (3C-like protease)variant Δ(298–306) | severe acute respiratory syndrome coronavirus (SARS-CoV) | ortho-aminobenzoic acid (ABZ)-TSAVLQSGFRK-2,4-dinitrophenyl amide (DNP); 11-mer peptide cleaved at the Q-S site | 6 × 10⁻⁴ | 0.03 | 0.020 calc. | pH 7.6, 30 °C | PMID 18275836 | Open record: SARS-CoV main protease (3C-like protease) variant Δ(298–306), ortho-aminobenzoic acid (ABZ)-TSAVLQSGFRK-2,4-dinitrophenyl amide (DNP); 11-mer peptide cleaved at the Q-S site |
| SARS-CoV main protease (3C-like protease)variant Δ(299–306) | severe acute respiratory syndrome coronavirus (SARS-CoV) | ortho-aminobenzoic acid (ABZ)-TSAVLQSGFRK-2,4-dinitrophenyl amide (DNP); 11-mer peptide cleaved at the Q-S site | 0.001 | 0.013 | 0.077 calc. | pH 7.6, 30 °C | PMID 18275836 | Open record: SARS-CoV main protease (3C-like protease) variant Δ(299–306), ortho-aminobenzoic acid (ABZ)-TSAVLQSGFRK-2,4-dinitrophenyl amide (DNP); 11-mer peptide cleaved at the Q-S site |
| SARS-CoV main protease (3C-like protease)variant Δ(300–306) | severe acute respiratory syndrome coronavirus (SARS-CoV) | ortho-aminobenzoic acid (ABZ)-TSAVLQSGFRK-2,4-dinitrophenyl amide (DNP); 11-mer peptide cleaved at the Q-S site | 0.017 | 0.015 | 1.1 calc. | pH 7.6, 30 °C | PMID 18275836 | Open record: SARS-CoV main protease (3C-like protease) variant Δ(300–306), ortho-aminobenzoic acid (ABZ)-TSAVLQSGFRK-2,4-dinitrophenyl amide (DNP); 11-mer peptide cleaved at the Q-S site |
| SARS-CoV main protease (3C-like protease)variant Δ(301–306) | severe acute respiratory syndrome coronavirus (SARS-CoV) | ortho-aminobenzoic acid (ABZ)-TSAVLQSGFRK-2,4-dinitrophenyl amide (DNP); 11-mer peptide cleaved at the Q-S site | 0.025 | 0.03 | 0.83 calc. | pH 7.6, 30 °C | PMID 18275836 | Open record: SARS-CoV main protease (3C-like protease) variant Δ(301–306), ortho-aminobenzoic acid (ABZ)-TSAVLQSGFRK-2,4-dinitrophenyl amide (DNP); 11-mer peptide cleaved at the Q-S site |
| SARS-CoV main protease (3C-like protease)variant Δ(302–306) | severe acute respiratory syndrome coronavirus (SARS-CoV) | ortho-aminobenzoic acid (ABZ)-TSAVLQSGFRK-2,4-dinitrophenyl amide (DNP); 11-mer peptide cleaved at the Q-S site | 0.03 | 0.022 | 1.4 calc. | pH 7.6, 30 °C | PMID 18275836 | Open record: SARS-CoV main protease (3C-like protease) variant Δ(302–306), ortho-aminobenzoic acid (ABZ)-TSAVLQSGFRK-2,4-dinitrophenyl amide (DNP); 11-mer peptide cleaved at the Q-S site |
| SARS-CoV main protease (3C-like protease)variant Δ(303–306) | severe acute respiratory syndrome coronavirus (SARS-CoV) | ortho-aminobenzoic acid (ABZ)-TSAVLQSGFRK-2,4-dinitrophenyl amide (DNP); 11-mer peptide cleaved at the Q-S site | 0.022 | 0.004 | 5.5 calc. | pH 7.6, 30 °C | PMID 18275836 | Open record: SARS-CoV main protease (3C-like protease) variant Δ(303–306), ortho-aminobenzoic acid (ABZ)-TSAVLQSGFRK-2,4-dinitrophenyl amide (DNP); 11-mer peptide cleaved at the Q-S site |
| SARS-CoV main protease (3C-like protease)variant Δ(304–306) | severe acute respiratory syndrome coronavirus (SARS-CoV) | ortho-aminobenzoic acid (ABZ)-TSAVLQSGFRK-2,4-dinitrophenyl amide (DNP); 11-mer peptide cleaved at the Q-S site | 0.027 | 0.006 | 4.5 calc. | pH 7.6, 30 °C | PMID 18275836 | Open record: SARS-CoV main protease (3C-like protease) variant Δ(304–306), ortho-aminobenzoic acid (ABZ)-TSAVLQSGFRK-2,4-dinitrophenyl amide (DNP); 11-mer peptide cleaved at the Q-S site |
| SARS-CoV main protease (3C-like protease)variant Δ(305−306) | severe acute respiratory syndrome coronavirus (SARS-CoV) | ortho-aminobenzoic acid (ABZ)-TSAVLQSGFRK-2,4-dinitrophenyl amide (DNP); 11-mer peptide cleaved at the Q-S site | 0.039 | 0.014 | 2.8 calc. | pH 7.6, 30 °C | PMID 18275836 | Open record: SARS-CoV main protease (3C-like protease) variant Δ(305−306), ortho-aminobenzoic acid (ABZ)-TSAVLQSGFRK-2,4-dinitrophenyl amide (DNP); 11-mer peptide cleaved at the Q-S site |
| SARS-CoV main protease (3C-like protease)variant Δ(306) | severe acute respiratory syndrome coronavirus (SARS-CoV) | ortho-aminobenzoic acid (ABZ)-TSAVLQSGFRK-2,4-dinitrophenyl amide (DNP); 11-mer peptide cleaved at the Q-S site | 0.043 | 0.021 | 2.0 calc. | pH 7.6, 30 °C | PMID 18275836 | Open record: SARS-CoV main protease (3C-like protease) variant Δ(306), ortho-aminobenzoic acid (ABZ)-TSAVLQSGFRK-2,4-dinitrophenyl amide (DNP); 11-mer peptide cleaved at the Q-S site |
| SARS-CoV main protease (Mpro) | SARS-CoV | TSAVLQ-para-nitroanilidesubstrate-titration assay in 10 mM phosphate buffer | 0.63 | 0.2226 | 2.83 | pH 7.6, 30 °C | PMID 20371333 | Open record: SARS-CoV main protease (Mpro), TSAVLQ-para-nitroanilide |
| SARS-CoV main protease (Mpro) | SARS-CoV | TSAVLQ-para-nitroanilideenzyme-concentration dependence in 10 mM phosphate buffer | 1.4 | — | — | pH 7.6, 30 °C | PMID 20371333 | Open record: SARS-CoV main protease (Mpro), TSAVLQ-para-nitroanilide |
| SARS-CoV main protease (Mpro)variant E166A | SARS-CoV | TSAVLQ-para-nitroanilide | 0.31 | 0.3534 | 0.877 | pH 7.6, 30 °C | PMID 20371333 | Open record: SARS-CoV main protease (Mpro) variant E166A, TSAVLQ-para-nitroanilide |
| SARS-CoV main protease (Mpro)variant R298A | SARS-CoV | TSAVLQ-para-nitroanilidesubstrate-titration assay in 10 mM phosphate buffer | 0.1 | — | — | pH 7.6, 30 °C | PMID 20371333 | Open record: SARS-CoV main protease (Mpro) variant R298A, TSAVLQ-para-nitroanilide |
| SARS-CoV main protease (Mpro)variant R298A | SARS-CoV | TSAVLQ-para-nitroanilideenzyme-concentration dependence in 10 mM phosphate buffer | 0.7 | — | — | pH 7.6, 30 °C | PMID 20371333 | Open record: SARS-CoV main protease (Mpro) variant R298A, TSAVLQ-para-nitroanilide |
| SARS-CoV main protease (Mpro)variant R298L | SARS-CoV | TSAVLQ-para-nitroanilidesubstrate-titration assay in 10 mM phosphate buffer | 0.48 | — | — | pH 7.6, 30 °C | PMID 20371333 | Open record: SARS-CoV main protease (Mpro) variant R298L, TSAVLQ-para-nitroanilide |
| SARS-CoV main protease (Mpro)variant R298L | SARS-CoV | TSAVLQ-para-nitroanilideenzyme-concentration dependence in 10 mM phosphate buffer | 2.3 | — | — | pH 7.6, 30 °C | PMID 20371333 | Open record: SARS-CoV main protease (Mpro) variant R298L, TSAVLQ-para-nitroanilide |
| TbCe1 (cytoplasmic capping enzyme, RNA kinase activity) | Trypanosoma brucei | 21-mer 5'-monophosphate RNA 5'-AACUAACGCUAUUAUUAGAAC-3' (first 21 nt of T. brucei SL RNA) with 2'-O-methyladenosine at A1 | 7.7 × 10⁻⁴ | — | — | pH 8.5, 27 °C | PMID 39011881 | Open record: TbCe1 (cytoplasmic capping enzyme, RNA kinase activity), 21-mer 5'-monophosphate RNA 5'-AACUAACGCUAUUAUUAGAAC-3' (first 21 nt of T. brucei SL RNA) with 2'-O-methyladenosine at A1 |
| TbCe1 (cytoplasmic capping enzyme, RNA kinase activity) | Trypanosoma brucei | 21-mer 5'-monophosphate RNA 5'-AACUAACGCUAUUAUUAGAAC-3' (first 21 nt of T. brucei SL RNA) with 2'-O-ribose methylations on A1, A2, C3 and U4 | 9.5 × 10⁻⁴ | — | — | pH 8.5, 27 °C | PMID 39011881 | Open record: TbCe1 (cytoplasmic capping enzyme, RNA kinase activity), 21-mer 5'-monophosphate RNA 5'-AACUAACGCUAUUAUUAGAAC-3' (first 21 nt of T. brucei SL RNA) with 2'-O-ribose methylations on A1, A2, C3 and U4 |
| TbCe1 (cytoplasmic capping enzyme, RNA kinase activity) | Trypanosoma brucei | 21-mer 5'-monophosphate RNA 5'-AACUAACGCUAUUAUUAGAAC-3' (first 21 nt of T. brucei SL RNA) with N1-methyladenosine at A1 | 1.2 × 10⁻⁴ | — | — | pH 8.5, 27 °C | PMID 39011881 | Open record: TbCe1 (cytoplasmic capping enzyme, RNA kinase activity), 21-mer 5'-monophosphate RNA 5'-AACUAACGCUAUUAUUAGAAC-3' (first 21 nt of T. brucei SL RNA) with N1-methyladenosine at A1 |
| TbCe1 (cytoplasmic capping enzyme, RNA kinase activity) | Trypanosoma brucei | 21-mer 5'-monophosphate RNA 5'-AACUAACGCUAUUAUUAGAAC-3' (first 21 nt of T. brucei SL RNA) with N6,N6,2'-O-trimethyladenosine at A1 | 0.03072 | — | — | pH 8.5, 27 °C | PMID 39011881 | Open record: TbCe1 (cytoplasmic capping enzyme, RNA kinase activity), 21-mer 5'-monophosphate RNA 5'-AACUAACGCUAUUAUUAGAAC-3' (first 21 nt of T. brucei SL RNA) with N6,N6,2'-O-trimethyladenosine at A1 |
| TbCe1 (cytoplasmic capping enzyme, RNA kinase activity) | Trypanosoma brucei | 21-mer 5'-monophosphate RNA 5'-AACUAACGCUAUUAUUAGAAC-3' (first 21 nt of T. brucei SL RNA) with N6,N6,2'-O-trimethyladenosine at A1 and 2'-O-ribose methylations on A2, C3 and U4 | 0.0264 | — | — | pH 8.5, 27 °C | PMID 39011881 | Open record: TbCe1 (cytoplasmic capping enzyme, RNA kinase activity), 21-mer 5'-monophosphate RNA 5'-AACUAACGCUAUUAUUAGAAC-3' (first 21 nt of T. brucei SL RNA) with N6,N6,2'-O-trimethyladenosine at A1 and 2'-O-ribose methylations on A2, C3 and U4 |
| TbCe1 (cytoplasmic capping enzyme, RNA kinase activity) | Trypanosoma brucei | 21-mer 5'-monophosphate RNA 5'-AACUAACGCUAUUAUUAGAAC-3' (first 21 nt of T. brucei SL RNA) with N6,N6,2'-O-trimethyladenosine at A1, 2'-O-methyl A2 and C3, N3,2'-O-dimethyluridine at U4 (decapped cap 4) | 0.038 | — | — | pH 8.5, 27 °C | PMID 39011881 | Open record: TbCe1 (cytoplasmic capping enzyme, RNA kinase activity), 21-mer 5'-monophosphate RNA 5'-AACUAACGCUAUUAUUAGAAC-3' (first 21 nt of T. brucei SL RNA) with N6,N6,2'-O-trimethyladenosine at A1, 2'-O-methyl A2 and C3, N3,2'-O-dimethyluridine at U4 (decapped cap 4) |
| TbCe1 (cytoplasmic capping enzyme, RNA kinase activity) | Trypanosoma brucei | 21-mer 5'-monophosphate RNA 5'-AACUAACGCUAUUAUUAGAAC-3' (first 21 nt of T. brucei SL RNA) with N6,N6-dimethyladenosine at A1 | 0.00353 | — | — | pH 8.5, 27 °C | PMID 39011881 | Open record: TbCe1 (cytoplasmic capping enzyme, RNA kinase activity), 21-mer 5'-monophosphate RNA 5'-AACUAACGCUAUUAUUAGAAC-3' (first 21 nt of T. brucei SL RNA) with N6,N6-dimethyladenosine at A1 |
| TbCe1 (cytoplasmic capping enzyme, RNA kinase activity) | Trypanosoma brucei | 21-mer 5'-monophosphate RNA 5'-AACUAACGCUAUUAUUAGAAC-3' (first 21 nt of T. brucei SL RNA) with N6-methyladenosine at A1 | 0.00145 | — | — | pH 8.5, 27 °C | PMID 39011881 | Open record: TbCe1 (cytoplasmic capping enzyme, RNA kinase activity), 21-mer 5'-monophosphate RNA 5'-AACUAACGCUAUUAUUAGAAC-3' (first 21 nt of T. brucei SL RNA) with N6-methyladenosine at A1 |
| TbCe1 (cytoplasmic capping enzyme, RNA kinase activity) | Trypanosoma brucei | 21-mer 5'-monophosphate RNA 5'-AACUAACGCUAUUAUUAGAAC-3' (first 21 nt of T. brucei SL RNA) with N6-methyladenosine at A1 and 2'-O-ribose methylations on C3 and U4 | 0.00127 | — | — | pH 8.5, 27 °C | PMID 39011881 | Open record: TbCe1 (cytoplasmic capping enzyme, RNA kinase activity), 21-mer 5'-monophosphate RNA 5'-AACUAACGCUAUUAUUAGAAC-3' (first 21 nt of T. brucei SL RNA) with N6-methyladenosine at A1 and 2'-O-ribose methylations on C3 and U4 |
| TbCe1 (cytoplasmic capping enzyme, RNA kinase activity) | Trypanosoma brucei | 21-mer 5'-monophosphate RNA 5'-AACUAACGCUAUUAUUAGAAC-3' (first 21 nt of T. brucei SL RNA), unmodified | 2.2 × 10⁻⁴ | — | — | pH 8.5, 27 °C | PMID 39011881 | Open record: TbCe1 (cytoplasmic capping enzyme, RNA kinase activity), 21-mer 5'-monophosphate RNA 5'-AACUAACGCUAUUAUUAGAAC-3' (first 21 nt of T. brucei SL RNA), unmodified |
| TbCe1 (cytoplasmic capping enzyme, RNA kinase activity) | Trypanosoma brucei | adenosine 5'-triphosphatep^{m6,2}A_mA_mC_m^{m3}U_m RNA | — | 2.1 × 10⁻⁴ | — | pH 8.5, 27 °C | PMID 39011881 | Open record: TbCe1 (cytoplasmic capping enzyme, RNA kinase activity), adenosine 5'-triphosphate |
| TbCe1 (cytoplasmic capping enzyme, RNA kinase activity) | Trypanosoma brucei | adenosine 5'-triphosphatepA_mA_mC_mU_m RNA | — | 0.00209 | — | pH 8.5, 27 °C | PMID 39011881 | Open record: TbCe1 (cytoplasmic capping enzyme, RNA kinase activity), adenosine 5'-triphosphate |
| TbCe1 (cytoplasmic capping enzyme, RNA kinase activity) | Trypanosoma brucei | adenosine 5'-triphosphatepA_m RNA | — | 0.00325 | — | pH 8.5, 27 °C | PMID 39011881 | Open record: TbCe1 (cytoplasmic capping enzyme, RNA kinase activity), adenosine 5'-triphosphate |
| TbCe1 (cytoplasmic capping enzyme, RNA kinase activity) | Trypanosoma brucei | adenosine 5'-triphosphateunmodified pRNA | — | 0.00532 | — | pH 8.5, 27 °C | PMID 39011881 | Open record: TbCe1 (cytoplasmic capping enzyme, RNA kinase activity), adenosine 5'-triphosphate |