Values CatLog read from this paper
Correlation between dissociation and catalysis of SARS-CoV main protease
PMID 18275836doi:10.1016/j.abb.2008.01.023Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 24 records (48 values) from this paper: SARS-CoV main protease (3C-like protease) from severe acute respiratory syndrome coronavirus (SARS-CoV).
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 24 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.
EC 2.7.7.50, EC 3.4.19.12, EC 3.4.22.69
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| SARS-CoV main protease (3C-like protease) | severe acute respiratory syndrome coronavirus (SARS-CoV) | ortho-aminobenzoic acid (ABZ)-TSAVLQSGFRK-2,4-dinitrophenyl amide (DNP); 11-mer peptide cleaved at the Q-S site | 0.032 | 0.011 | 2.9 calc. | pH 7.6, 30 °C | Open record: SARS-CoV main protease (3C-like protease), ortho-aminobenzoic acid (ABZ)-TSAVLQSGFRK-2,4-dinitrophenyl amide (DNP); 11-mer peptide cleaved at the Q-S site |
| SARS-CoV main protease (3C-like protease)variant Q299A | severe acute respiratory syndrome coronavirus (SARS-CoV) | ortho-aminobenzoic acid (ABZ)-TSAVLQSGFRK-2,4-dinitrophenyl amide (DNP); 11-mer peptide cleaved at the Q-S site | 7 × 10⁻⁴ | 0.018 | 0.039 calc. | pH 7.6, 30 °C | Open record: SARS-CoV main protease (3C-like protease) variant Q299A, ortho-aminobenzoic acid (ABZ)-TSAVLQSGFRK-2,4-dinitrophenyl amide (DNP); 11-mer peptide cleaved at the Q-S site |
| SARS-CoV main protease (3C-like protease)variant Q299E | severe acute respiratory syndrome coronavirus (SARS-CoV) | ortho-aminobenzoic acid (ABZ)-TSAVLQSGFRK-2,4-dinitrophenyl amide (DNP); 11-mer peptide cleaved at the Q-S site | 0.0022 | 0.005 | 0.44 calc. | pH 7.6, 30 °C | Open record: SARS-CoV main protease (3C-like protease) variant Q299E, ortho-aminobenzoic acid (ABZ)-TSAVLQSGFRK-2,4-dinitrophenyl amide (DNP); 11-mer peptide cleaved at the Q-S site |
| SARS-CoV main protease (3C-like protease)variant Q299K | severe acute respiratory syndrome coronavirus (SARS-CoV) | ortho-aminobenzoic acid (ABZ)-TSAVLQSGFRK-2,4-dinitrophenyl amide (DNP); 11-mer peptide cleaved at the Q-S site | 0.0021 | 0.037 | 0.057 calc. | pH 7.6, 30 °C | Open record: SARS-CoV main protease (3C-like protease) variant Q299K, ortho-aminobenzoic acid (ABZ)-TSAVLQSGFRK-2,4-dinitrophenyl amide (DNP); 11-mer peptide cleaved at the Q-S site |
| SARS-CoV main protease (3C-like protease)variant Q299N | severe acute respiratory syndrome coronavirus (SARS-CoV) | ortho-aminobenzoic acid (ABZ)-TSAVLQSGFRK-2,4-dinitrophenyl amide (DNP); 11-mer peptide cleaved at the Q-S site | 0.0017 | 0.005 | 0.34 calc. | pH 7.6, 30 °C | Open record: SARS-CoV main protease (3C-like protease) variant Q299N, ortho-aminobenzoic acid (ABZ)-TSAVLQSGFRK-2,4-dinitrophenyl amide (DNP); 11-mer peptide cleaved at the Q-S site |
| SARS-CoV main protease (3C-like protease)variant R298A | severe acute respiratory syndrome coronavirus (SARS-CoV) | ortho-aminobenzoic acid (ABZ)-TSAVLQSGFRK-2,4-dinitrophenyl amide (DNP); 11-mer peptide cleaved at the Q-S site | 0.0034 | 0.011 | 0.31 calc. | pH 7.6, 30 °C | Open record: SARS-CoV main protease (3C-like protease) variant R298A, ortho-aminobenzoic acid (ABZ)-TSAVLQSGFRK-2,4-dinitrophenyl amide (DNP); 11-mer peptide cleaved at the Q-S site |
| SARS-CoV main protease (3C-like protease)variant R298A/Q299A | severe acute respiratory syndrome coronavirus (SARS-CoV) | ortho-aminobenzoic acid (ABZ)-TSAVLQSGFRK-2,4-dinitrophenyl amide (DNP); 11-mer peptide cleaved at the Q-S site | 2.1 × 10⁻⁴ | 0.01 | 0.021 calc. | pH 7.6, 30 °C | Open record: SARS-CoV main protease (3C-like protease) variant R298A/Q299A, ortho-aminobenzoic acid (ABZ)-TSAVLQSGFRK-2,4-dinitrophenyl amide (DNP); 11-mer peptide cleaved at the Q-S site |
| SARS-CoV main protease (3C-like protease)variant R298K | severe acute respiratory syndrome coronavirus (SARS-CoV) | ortho-aminobenzoic acid (ABZ)-TSAVLQSGFRK-2,4-dinitrophenyl amide (DNP); 11-mer peptide cleaved at the Q-S site | 0.017 | 0.016 | 1.1 calc. | pH 7.6, 30 °C | Open record: SARS-CoV main protease (3C-like protease) variant R298K, ortho-aminobenzoic acid (ABZ)-TSAVLQSGFRK-2,4-dinitrophenyl amide (DNP); 11-mer peptide cleaved at the Q-S site |
| SARS-CoV main protease (3C-like protease)variant R298L | severe acute respiratory syndrome coronavirus (SARS-CoV) | ortho-aminobenzoic acid (ABZ)-TSAVLQSGFRK-2,4-dinitrophenyl amide (DNP); 11-mer peptide cleaved at the Q-S site | 0.0048 | 0.013 | 0.37 calc. | pH 7.6, 30 °C | Open record: SARS-CoV main protease (3C-like protease) variant R298L, ortho-aminobenzoic acid (ABZ)-TSAVLQSGFRK-2,4-dinitrophenyl amide (DNP); 11-mer peptide cleaved at the Q-S site |
| SARS-CoV main protease (3C-like protease)variant S123A | severe acute respiratory syndrome coronavirus (SARS-CoV) | ortho-aminobenzoic acid (ABZ)-TSAVLQSGFRK-2,4-dinitrophenyl amide (DNP); 11-mer peptide cleaved at the Q-S site | 0.033 | 0.013 | 2.5 calc. | pH 7.6, 30 °C | Open record: SARS-CoV main protease (3C-like protease) variant S123A, ortho-aminobenzoic acid (ABZ)-TSAVLQSGFRK-2,4-dinitrophenyl amide (DNP); 11-mer peptide cleaved at the Q-S site |
| SARS-CoV main protease (3C-like protease)variant S123A/R298A | severe acute respiratory syndrome coronavirus (SARS-CoV) | ortho-aminobenzoic acid (ABZ)-TSAVLQSGFRK-2,4-dinitrophenyl amide (DNP); 11-mer peptide cleaved at the Q-S site | 0.0069 | 0.012 | 0.57 calc. | pH 7.6, 30 °C | Open record: SARS-CoV main protease (3C-like protease) variant S123A/R298A, ortho-aminobenzoic acid (ABZ)-TSAVLQSGFRK-2,4-dinitrophenyl amide (DNP); 11-mer peptide cleaved at the Q-S site |
| SARS-CoV main protease (3C-like protease)variant S123C | severe acute respiratory syndrome coronavirus (SARS-CoV) | ortho-aminobenzoic acid (ABZ)-TSAVLQSGFRK-2,4-dinitrophenyl amide (DNP); 11-mer peptide cleaved at the Q-S site | 0.035 | 0.013 | 2.7 calc. | pH 7.6, 30 °C | Open record: SARS-CoV main protease (3C-like protease) variant S123C, ortho-aminobenzoic acid (ABZ)-TSAVLQSGFRK-2,4-dinitrophenyl amide (DNP); 11-mer peptide cleaved at the Q-S site |
| SARS-CoV main protease (3C-like protease)variant S139A | severe acute respiratory syndrome coronavirus (SARS-CoV) | ortho-aminobenzoic acid (ABZ)-TSAVLQSGFRK-2,4-dinitrophenyl amide (DNP); 11-mer peptide cleaved at the Q-S site | 0.033 | 0.01 | 3.3 calc. | pH 7.6, 30 °C | Open record: SARS-CoV main protease (3C-like protease) variant S139A, ortho-aminobenzoic acid (ABZ)-TSAVLQSGFRK-2,4-dinitrophenyl amide (DNP); 11-mer peptide cleaved at the Q-S site |
| SARS-CoV main protease (3C-like protease)variant S139A/Q299A | severe acute respiratory syndrome coronavirus (SARS-CoV) | ortho-aminobenzoic acid (ABZ)-TSAVLQSGFRK-2,4-dinitrophenyl amide (DNP); 11-mer peptide cleaved at the Q-S site | 0.0048 | 0.021 | 0.23 calc. | pH 7.6, 30 °C | Open record: SARS-CoV main protease (3C-like protease) variant S139A/Q299A, ortho-aminobenzoic acid (ABZ)-TSAVLQSGFRK-2,4-dinitrophenyl amide (DNP); 11-mer peptide cleaved at the Q-S site |
| SARS-CoV main protease (3C-like protease)variant Δ(297–306) | severe acute respiratory syndrome coronavirus (SARS-CoV) | ortho-aminobenzoic acid (ABZ)-TSAVLQSGFRK-2,4-dinitrophenyl amide (DNP); 11-mer peptide cleaved at the Q-S site | 4 × 10⁻⁵ | 0.021 | 0.0019 calc. | pH 7.6, 30 °C | Open record: SARS-CoV main protease (3C-like protease) variant Δ(297–306), ortho-aminobenzoic acid (ABZ)-TSAVLQSGFRK-2,4-dinitrophenyl amide (DNP); 11-mer peptide cleaved at the Q-S site |
| SARS-CoV main protease (3C-like protease)variant Δ(298–306) | severe acute respiratory syndrome coronavirus (SARS-CoV) | ortho-aminobenzoic acid (ABZ)-TSAVLQSGFRK-2,4-dinitrophenyl amide (DNP); 11-mer peptide cleaved at the Q-S site | 6 × 10⁻⁴ | 0.03 | 0.020 calc. | pH 7.6, 30 °C | Open record: SARS-CoV main protease (3C-like protease) variant Δ(298–306), ortho-aminobenzoic acid (ABZ)-TSAVLQSGFRK-2,4-dinitrophenyl amide (DNP); 11-mer peptide cleaved at the Q-S site |
| SARS-CoV main protease (3C-like protease)variant Δ(299–306) | severe acute respiratory syndrome coronavirus (SARS-CoV) | ortho-aminobenzoic acid (ABZ)-TSAVLQSGFRK-2,4-dinitrophenyl amide (DNP); 11-mer peptide cleaved at the Q-S site | 0.001 | 0.013 | 0.077 calc. | pH 7.6, 30 °C | Open record: SARS-CoV main protease (3C-like protease) variant Δ(299–306), ortho-aminobenzoic acid (ABZ)-TSAVLQSGFRK-2,4-dinitrophenyl amide (DNP); 11-mer peptide cleaved at the Q-S site |
| SARS-CoV main protease (3C-like protease)variant Δ(300–306) | severe acute respiratory syndrome coronavirus (SARS-CoV) | ortho-aminobenzoic acid (ABZ)-TSAVLQSGFRK-2,4-dinitrophenyl amide (DNP); 11-mer peptide cleaved at the Q-S site | 0.017 | 0.015 | 1.1 calc. | pH 7.6, 30 °C | Open record: SARS-CoV main protease (3C-like protease) variant Δ(300–306), ortho-aminobenzoic acid (ABZ)-TSAVLQSGFRK-2,4-dinitrophenyl amide (DNP); 11-mer peptide cleaved at the Q-S site |
| SARS-CoV main protease (3C-like protease)variant Δ(301–306) | severe acute respiratory syndrome coronavirus (SARS-CoV) | ortho-aminobenzoic acid (ABZ)-TSAVLQSGFRK-2,4-dinitrophenyl amide (DNP); 11-mer peptide cleaved at the Q-S site | 0.025 | 0.03 | 0.83 calc. | pH 7.6, 30 °C | Open record: SARS-CoV main protease (3C-like protease) variant Δ(301–306), ortho-aminobenzoic acid (ABZ)-TSAVLQSGFRK-2,4-dinitrophenyl amide (DNP); 11-mer peptide cleaved at the Q-S site |
| SARS-CoV main protease (3C-like protease)variant Δ(302–306) | severe acute respiratory syndrome coronavirus (SARS-CoV) | ortho-aminobenzoic acid (ABZ)-TSAVLQSGFRK-2,4-dinitrophenyl amide (DNP); 11-mer peptide cleaved at the Q-S site | 0.03 | 0.022 | 1.4 calc. | pH 7.6, 30 °C | Open record: SARS-CoV main protease (3C-like protease) variant Δ(302–306), ortho-aminobenzoic acid (ABZ)-TSAVLQSGFRK-2,4-dinitrophenyl amide (DNP); 11-mer peptide cleaved at the Q-S site |
| SARS-CoV main protease (3C-like protease)variant Δ(303–306) | severe acute respiratory syndrome coronavirus (SARS-CoV) | ortho-aminobenzoic acid (ABZ)-TSAVLQSGFRK-2,4-dinitrophenyl amide (DNP); 11-mer peptide cleaved at the Q-S site | 0.022 | 0.004 | 5.5 calc. | pH 7.6, 30 °C | Open record: SARS-CoV main protease (3C-like protease) variant Δ(303–306), ortho-aminobenzoic acid (ABZ)-TSAVLQSGFRK-2,4-dinitrophenyl amide (DNP); 11-mer peptide cleaved at the Q-S site |
| SARS-CoV main protease (3C-like protease)variant Δ(304–306) | severe acute respiratory syndrome coronavirus (SARS-CoV) | ortho-aminobenzoic acid (ABZ)-TSAVLQSGFRK-2,4-dinitrophenyl amide (DNP); 11-mer peptide cleaved at the Q-S site | 0.027 | 0.006 | 4.5 calc. | pH 7.6, 30 °C | Open record: SARS-CoV main protease (3C-like protease) variant Δ(304–306), ortho-aminobenzoic acid (ABZ)-TSAVLQSGFRK-2,4-dinitrophenyl amide (DNP); 11-mer peptide cleaved at the Q-S site |
| SARS-CoV main protease (3C-like protease)variant Δ(305−306) | severe acute respiratory syndrome coronavirus (SARS-CoV) | ortho-aminobenzoic acid (ABZ)-TSAVLQSGFRK-2,4-dinitrophenyl amide (DNP); 11-mer peptide cleaved at the Q-S site | 0.039 | 0.014 | 2.8 calc. | pH 7.6, 30 °C | Open record: SARS-CoV main protease (3C-like protease) variant Δ(305−306), ortho-aminobenzoic acid (ABZ)-TSAVLQSGFRK-2,4-dinitrophenyl amide (DNP); 11-mer peptide cleaved at the Q-S site |
| SARS-CoV main protease (3C-like protease)variant Δ(306) | severe acute respiratory syndrome coronavirus (SARS-CoV) | ortho-aminobenzoic acid (ABZ)-TSAVLQSGFRK-2,4-dinitrophenyl amide (DNP); 11-mer peptide cleaved at the Q-S site | 0.043 | 0.021 | 2.0 calc. | pH 7.6, 30 °C | Open record: SARS-CoV main protease (3C-like protease) variant Δ(306), ortho-aminobenzoic acid (ABZ)-TSAVLQSGFRK-2,4-dinitrophenyl amide (DNP); 11-mer peptide cleaved at the Q-S site |