EC 3.4.19.12: ubiquitinyl hydrolase 1
HydrolasesOpen in Browse
CatLog read 92 records for EC 3.4.19.12 from 7 papers, for 6 organisms. Names in these papers: SARS-CoV main protease (3C-like protease), ubiquitin carboxy-terminal hydrolase L1 (UCH-L1), ubiquitin carboxy-terminal hydrolase L3 (UCH-L3), nsp13 helicase and 4 more.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units.
CatLog also holds 40 records for EC 3.4.19.12 that are not yet re-read: from its earlier version, built from the enzyme databases and partly checked against the papers. All records for EC 3.4.19.12 in Browse.
Papers
| Paper | Organisms | Records |
|---|---|---|
| Substrate recognition and catalysis by UCH-L1 | — | 38 |
| Correlation between dissociation and catalysis of SARS-CoV main protease | severe acute respiratory syndrome coronavirus (SARS-CoV) | 24 |
| Motif V is an allosteric couple between the SARS-CoV-2 nsp13 nucleotide triphosphatase and helicase active sites | SARS-CoV-2 | 12 |
| Mutation of Glu-166 blocks the substrate-induced dimerization of SARS coronavirus main protease | SARS-CoV | 7 |
| Structural and Biochemical Characterization of Porcine Epidemic Diarrhea Virus Papain-Like Protease 2 | Porcine epidemic diarrhea virus | 7 |
| Remdesivir is a direct-acting antiviral that inhibits RNA-dependent RNA polymerase from severe acute respiratory syndrome coronavirus 2 with high potency | Severe acute respiratory syndrome coronavirus | 2 |
| Structural insights into ubiquitin recognition by USP15 revealed through a covalent activity-based probe | Homo sapiens | 2 |
Records
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Paper | Open record |
|---|---|---|---|---|---|---|---|---|
| nsp13 helicase | SARS-CoV-2 | ATP100 nM labeled dsDNA | 1.4 | 0.122 | 13 | pH 7.5, 37 °C | PMID 41581884 | Open record: nsp13 helicase, ATP |
| nsp13 helicase | SARS-CoV-2 | ATPunlabeled dsDNA present | 0.37 | 0.156 | 2.7 | pH 7.5, 37 °C | PMID 41581884 | Open record: nsp13 helicase, ATP |
| nsp13 helicase | SARS-CoV-2 | dsDNA | 1.5 | 2.85 × 10⁻⁴ | 6 × 10³ | pH 7.5, 37 °C | PMID 41581884 | Open record: nsp13 helicase, dsDNA |
| nsp13 helicasevariant D534A | SARS-CoV-2 | ATP | 0.26 | 0.135 | 1.9 | pH 7.5, 37 °C | PMID 41581884 | Open record: nsp13 helicase variant D534A, ATP |
| nsp13 helicasevariant L405D | SARS-CoV-2 | ATP | 0.9 | 0.118 | 10 | pH 7.5, 37 °C | PMID 41581884 | Open record: nsp13 helicase variant L405D, ATP |
| nsp13 helicasevariant L405D | SARS-CoV-2 | dsDNA | 10 | — | 3 × 10³ | pH 7.5, 37 °C | PMID 41581884 | Open record: nsp13 helicase variant L405D, dsDNA |
| nsp13 helicasevariant S535A | SARS-CoV-2 | ATP100 nM labeled dsDNA | 2.8 | 0.094 | 30 | pH 7.5, 37 °C | PMID 41581884 | Open record: nsp13 helicase variant S535A, ATP |
| nsp13 helicasevariant S535A | SARS-CoV-2 | ATPunlabeled dsDNA present | 0.47 | 0.176 | 2.7 | pH 7.5, 37 °C | PMID 41581884 | Open record: nsp13 helicase variant S535A, ATP |
| nsp13 helicasevariant S535A | SARS-CoV-2 | dsDNA | 2.4 | 1.75 × 10⁻⁴ | 1.6 × 10⁴ | pH 7.5, 37 °C | PMID 41581884 | Open record: nsp13 helicase variant S535A, dsDNA |
| nsp13 helicasevariant T532A | SARS-CoV-2 | ATP100 nM labeled dsDNA | 2.9 | 0.121 | 24 | pH 7.5, 37 °C | PMID 41581884 | Open record: nsp13 helicase variant T532A, ATP |
| nsp13 helicasevariant T532A | SARS-CoV-2 | ATPunlabeled dsDNA present | 0.61 | 0.126 | 5 | pH 7.5, 37 °C | PMID 41581884 | Open record: nsp13 helicase variant T532A, ATP |
| nsp13 helicasevariant T532A | SARS-CoV-2 | dsDNA | 3.7 | 2.82 × 10⁻⁴ | 1.4 × 10⁴ | pH 7.5, 37 °C | PMID 41581884 | Open record: nsp13 helicase variant T532A, dsDNA |
| PEDV PLP2variant E99A | Porcine epidemic diarrhea virus | Dabcyl-FKKKGGGDVKE-Edans | 6.2 × 10⁻⁴ | 0.0142 | 0.043 | pH 6.5, 30 °C | PMID 34643430 | Open record: PEDV PLP2 variant E99A, Dabcyl-FKKKGGGDVKE-Edans |
| PEDV PLP2variant E99A | Porcine epidemic diarrhea virus | Dabcyl-FRLKGGAPIKGV-Edans | 2.3 × 10⁻⁴ | 0.0048 | 0.048 | pH 6.5, 30 °C | PMID 34643430 | Open record: PEDV PLP2 variant E99A, Dabcyl-FRLKGGAPIKGV-Edans |
| PEDV PLP2variant N100A | Porcine epidemic diarrhea virus | Dabcyl-FKKKGGGDVKE-Edans | 0.0045 | 0.0462 | 0.097 | pH 6.5, 30 °C | PMID 34643430 | Open record: PEDV PLP2 variant N100A, Dabcyl-FKKKGGGDVKE-Edans |
| PEDV PLP2variant N103A | Porcine epidemic diarrhea virus | Dabcyl-FKKKGGGDVKE-Edans | 0.0055 | 0.0451 | 0.122 | pH 6.5, 30 °C | PMID 34643430 | Open record: PEDV PLP2 variant N103A, Dabcyl-FKKKGGGDVKE-Edans |
| PEDV PLP2variant N138A | Porcine epidemic diarrhea virus | Dabcyl-FKKKGGGDVKE-Edans | 0.0085 | 0.0434 | 0.197 | pH 6.5, 30 °C | PMID 34643430 | Open record: PEDV PLP2 variant N138A, Dabcyl-FKKKGGGDVKE-Edans |
| PEDV PLP2variant Δ195-196 | Porcine epidemic diarrhea virus | Dabcyl-FKKKGGGDVKE-Edans | 0.0033 | 0.0249 | 0.133 | pH 6.5, 30 °C | PMID 34643430 | Open record: PEDV PLP2 variant Δ195-196, Dabcyl-FKKKGGGDVKE-Edans |
| PEDV PLP2variant Δ195-196 | Porcine epidemic diarrhea virus | Dabcyl-FRLKGGAPIKGV-Edans | 5 × 10⁻⁴ | 0.0217 | 0.025 | pH 6.5, 30 °C | PMID 34643430 | Open record: PEDV PLP2 variant Δ195-196, Dabcyl-FRLKGGAPIKGV-Edans |
| RNA-dependent RNA polymerase (nsp12/nsp8 complex) | Severe acute respiratory syndrome coronavirus | adenosine 5'-triphosphate | — | 3 × 10⁻⁵ | — | pH 8, 30 °C | PMID 32284326 | Open record: RNA-dependent RNA polymerase (nsp12/nsp8 complex), adenosine 5'-triphosphate |
| RNA-dependent RNA polymerase (nsp12/nsp8 complex) | Severe acute respiratory syndrome coronavirus | remdesivir triphosphate | — | 1 × 10⁻⁵ | — | pH 8, 30 °C | PMID 32284326 | Open record: RNA-dependent RNA polymerase (nsp12/nsp8 complex), remdesivir triphosphate |
| SARS-CoV main protease (3C-like protease) | severe acute respiratory syndrome coronavirus (SARS-CoV) | ortho-aminobenzoic acid (ABZ)-TSAVLQSGFRK-2,4-dinitrophenyl amide (DNP); 11-mer peptide cleaved at the Q-S site | 0.032 | 0.011 | 2.9 calc. | pH 7.6, 30 °C | PMID 18275836 | Open record: SARS-CoV main protease (3C-like protease), ortho-aminobenzoic acid (ABZ)-TSAVLQSGFRK-2,4-dinitrophenyl amide (DNP); 11-mer peptide cleaved at the Q-S site |
| SARS-CoV main protease (3C-like protease)variant Q299A | severe acute respiratory syndrome coronavirus (SARS-CoV) | ortho-aminobenzoic acid (ABZ)-TSAVLQSGFRK-2,4-dinitrophenyl amide (DNP); 11-mer peptide cleaved at the Q-S site | 7 × 10⁻⁴ | 0.018 | 0.039 calc. | pH 7.6, 30 °C | PMID 18275836 | Open record: SARS-CoV main protease (3C-like protease) variant Q299A, ortho-aminobenzoic acid (ABZ)-TSAVLQSGFRK-2,4-dinitrophenyl amide (DNP); 11-mer peptide cleaved at the Q-S site |
| SARS-CoV main protease (3C-like protease)variant Q299E | severe acute respiratory syndrome coronavirus (SARS-CoV) | ortho-aminobenzoic acid (ABZ)-TSAVLQSGFRK-2,4-dinitrophenyl amide (DNP); 11-mer peptide cleaved at the Q-S site | 0.0022 | 0.005 | 0.44 calc. | pH 7.6, 30 °C | PMID 18275836 | Open record: SARS-CoV main protease (3C-like protease) variant Q299E, ortho-aminobenzoic acid (ABZ)-TSAVLQSGFRK-2,4-dinitrophenyl amide (DNP); 11-mer peptide cleaved at the Q-S site |
| SARS-CoV main protease (3C-like protease)variant Q299K | severe acute respiratory syndrome coronavirus (SARS-CoV) | ortho-aminobenzoic acid (ABZ)-TSAVLQSGFRK-2,4-dinitrophenyl amide (DNP); 11-mer peptide cleaved at the Q-S site | 0.0021 | 0.037 | 0.057 calc. | pH 7.6, 30 °C | PMID 18275836 | Open record: SARS-CoV main protease (3C-like protease) variant Q299K, ortho-aminobenzoic acid (ABZ)-TSAVLQSGFRK-2,4-dinitrophenyl amide (DNP); 11-mer peptide cleaved at the Q-S site |
| SARS-CoV main protease (3C-like protease)variant Q299N | severe acute respiratory syndrome coronavirus (SARS-CoV) | ortho-aminobenzoic acid (ABZ)-TSAVLQSGFRK-2,4-dinitrophenyl amide (DNP); 11-mer peptide cleaved at the Q-S site | 0.0017 | 0.005 | 0.34 calc. | pH 7.6, 30 °C | PMID 18275836 | Open record: SARS-CoV main protease (3C-like protease) variant Q299N, ortho-aminobenzoic acid (ABZ)-TSAVLQSGFRK-2,4-dinitrophenyl amide (DNP); 11-mer peptide cleaved at the Q-S site |
| SARS-CoV main protease (3C-like protease)variant R298A | severe acute respiratory syndrome coronavirus (SARS-CoV) | ortho-aminobenzoic acid (ABZ)-TSAVLQSGFRK-2,4-dinitrophenyl amide (DNP); 11-mer peptide cleaved at the Q-S site | 0.0034 | 0.011 | 0.31 calc. | pH 7.6, 30 °C | PMID 18275836 | Open record: SARS-CoV main protease (3C-like protease) variant R298A, ortho-aminobenzoic acid (ABZ)-TSAVLQSGFRK-2,4-dinitrophenyl amide (DNP); 11-mer peptide cleaved at the Q-S site |
| SARS-CoV main protease (3C-like protease)variant R298A/Q299A | severe acute respiratory syndrome coronavirus (SARS-CoV) | ortho-aminobenzoic acid (ABZ)-TSAVLQSGFRK-2,4-dinitrophenyl amide (DNP); 11-mer peptide cleaved at the Q-S site | 2.1 × 10⁻⁴ | 0.01 | 0.021 calc. | pH 7.6, 30 °C | PMID 18275836 | Open record: SARS-CoV main protease (3C-like protease) variant R298A/Q299A, ortho-aminobenzoic acid (ABZ)-TSAVLQSGFRK-2,4-dinitrophenyl amide (DNP); 11-mer peptide cleaved at the Q-S site |
| SARS-CoV main protease (3C-like protease)variant R298K | severe acute respiratory syndrome coronavirus (SARS-CoV) | ortho-aminobenzoic acid (ABZ)-TSAVLQSGFRK-2,4-dinitrophenyl amide (DNP); 11-mer peptide cleaved at the Q-S site | 0.017 | 0.016 | 1.1 calc. | pH 7.6, 30 °C | PMID 18275836 | Open record: SARS-CoV main protease (3C-like protease) variant R298K, ortho-aminobenzoic acid (ABZ)-TSAVLQSGFRK-2,4-dinitrophenyl amide (DNP); 11-mer peptide cleaved at the Q-S site |
| SARS-CoV main protease (3C-like protease)variant R298L | severe acute respiratory syndrome coronavirus (SARS-CoV) | ortho-aminobenzoic acid (ABZ)-TSAVLQSGFRK-2,4-dinitrophenyl amide (DNP); 11-mer peptide cleaved at the Q-S site | 0.0048 | 0.013 | 0.37 calc. | pH 7.6, 30 °C | PMID 18275836 | Open record: SARS-CoV main protease (3C-like protease) variant R298L, ortho-aminobenzoic acid (ABZ)-TSAVLQSGFRK-2,4-dinitrophenyl amide (DNP); 11-mer peptide cleaved at the Q-S site |
| SARS-CoV main protease (3C-like protease)variant S123A | severe acute respiratory syndrome coronavirus (SARS-CoV) | ortho-aminobenzoic acid (ABZ)-TSAVLQSGFRK-2,4-dinitrophenyl amide (DNP); 11-mer peptide cleaved at the Q-S site | 0.033 | 0.013 | 2.5 calc. | pH 7.6, 30 °C | PMID 18275836 | Open record: SARS-CoV main protease (3C-like protease) variant S123A, ortho-aminobenzoic acid (ABZ)-TSAVLQSGFRK-2,4-dinitrophenyl amide (DNP); 11-mer peptide cleaved at the Q-S site |
| SARS-CoV main protease (3C-like protease)variant S123A/R298A | severe acute respiratory syndrome coronavirus (SARS-CoV) | ortho-aminobenzoic acid (ABZ)-TSAVLQSGFRK-2,4-dinitrophenyl amide (DNP); 11-mer peptide cleaved at the Q-S site | 0.0069 | 0.012 | 0.57 calc. | pH 7.6, 30 °C | PMID 18275836 | Open record: SARS-CoV main protease (3C-like protease) variant S123A/R298A, ortho-aminobenzoic acid (ABZ)-TSAVLQSGFRK-2,4-dinitrophenyl amide (DNP); 11-mer peptide cleaved at the Q-S site |
| SARS-CoV main protease (3C-like protease)variant S123C | severe acute respiratory syndrome coronavirus (SARS-CoV) | ortho-aminobenzoic acid (ABZ)-TSAVLQSGFRK-2,4-dinitrophenyl amide (DNP); 11-mer peptide cleaved at the Q-S site | 0.035 | 0.013 | 2.7 calc. | pH 7.6, 30 °C | PMID 18275836 | Open record: SARS-CoV main protease (3C-like protease) variant S123C, ortho-aminobenzoic acid (ABZ)-TSAVLQSGFRK-2,4-dinitrophenyl amide (DNP); 11-mer peptide cleaved at the Q-S site |
| SARS-CoV main protease (3C-like protease)variant S139A | severe acute respiratory syndrome coronavirus (SARS-CoV) | ortho-aminobenzoic acid (ABZ)-TSAVLQSGFRK-2,4-dinitrophenyl amide (DNP); 11-mer peptide cleaved at the Q-S site | 0.033 | 0.01 | 3.3 calc. | pH 7.6, 30 °C | PMID 18275836 | Open record: SARS-CoV main protease (3C-like protease) variant S139A, ortho-aminobenzoic acid (ABZ)-TSAVLQSGFRK-2,4-dinitrophenyl amide (DNP); 11-mer peptide cleaved at the Q-S site |
| SARS-CoV main protease (3C-like protease)variant S139A/Q299A | severe acute respiratory syndrome coronavirus (SARS-CoV) | ortho-aminobenzoic acid (ABZ)-TSAVLQSGFRK-2,4-dinitrophenyl amide (DNP); 11-mer peptide cleaved at the Q-S site | 0.0048 | 0.021 | 0.23 calc. | pH 7.6, 30 °C | PMID 18275836 | Open record: SARS-CoV main protease (3C-like protease) variant S139A/Q299A, ortho-aminobenzoic acid (ABZ)-TSAVLQSGFRK-2,4-dinitrophenyl amide (DNP); 11-mer peptide cleaved at the Q-S site |
| SARS-CoV main protease (3C-like protease)variant Δ(297–306) | severe acute respiratory syndrome coronavirus (SARS-CoV) | ortho-aminobenzoic acid (ABZ)-TSAVLQSGFRK-2,4-dinitrophenyl amide (DNP); 11-mer peptide cleaved at the Q-S site | 4 × 10⁻⁵ | 0.021 | 0.0019 calc. | pH 7.6, 30 °C | PMID 18275836 | Open record: SARS-CoV main protease (3C-like protease) variant Δ(297–306), ortho-aminobenzoic acid (ABZ)-TSAVLQSGFRK-2,4-dinitrophenyl amide (DNP); 11-mer peptide cleaved at the Q-S site |
| SARS-CoV main protease (3C-like protease)variant Δ(298–306) | severe acute respiratory syndrome coronavirus (SARS-CoV) | ortho-aminobenzoic acid (ABZ)-TSAVLQSGFRK-2,4-dinitrophenyl amide (DNP); 11-mer peptide cleaved at the Q-S site | 6 × 10⁻⁴ | 0.03 | 0.020 calc. | pH 7.6, 30 °C | PMID 18275836 | Open record: SARS-CoV main protease (3C-like protease) variant Δ(298–306), ortho-aminobenzoic acid (ABZ)-TSAVLQSGFRK-2,4-dinitrophenyl amide (DNP); 11-mer peptide cleaved at the Q-S site |
| SARS-CoV main protease (3C-like protease)variant Δ(299–306) | severe acute respiratory syndrome coronavirus (SARS-CoV) | ortho-aminobenzoic acid (ABZ)-TSAVLQSGFRK-2,4-dinitrophenyl amide (DNP); 11-mer peptide cleaved at the Q-S site | 0.001 | 0.013 | 0.077 calc. | pH 7.6, 30 °C | PMID 18275836 | Open record: SARS-CoV main protease (3C-like protease) variant Δ(299–306), ortho-aminobenzoic acid (ABZ)-TSAVLQSGFRK-2,4-dinitrophenyl amide (DNP); 11-mer peptide cleaved at the Q-S site |
| SARS-CoV main protease (3C-like protease)variant Δ(300–306) | severe acute respiratory syndrome coronavirus (SARS-CoV) | ortho-aminobenzoic acid (ABZ)-TSAVLQSGFRK-2,4-dinitrophenyl amide (DNP); 11-mer peptide cleaved at the Q-S site | 0.017 | 0.015 | 1.1 calc. | pH 7.6, 30 °C | PMID 18275836 | Open record: SARS-CoV main protease (3C-like protease) variant Δ(300–306), ortho-aminobenzoic acid (ABZ)-TSAVLQSGFRK-2,4-dinitrophenyl amide (DNP); 11-mer peptide cleaved at the Q-S site |
| SARS-CoV main protease (3C-like protease)variant Δ(301–306) | severe acute respiratory syndrome coronavirus (SARS-CoV) | ortho-aminobenzoic acid (ABZ)-TSAVLQSGFRK-2,4-dinitrophenyl amide (DNP); 11-mer peptide cleaved at the Q-S site | 0.025 | 0.03 | 0.83 calc. | pH 7.6, 30 °C | PMID 18275836 | Open record: SARS-CoV main protease (3C-like protease) variant Δ(301–306), ortho-aminobenzoic acid (ABZ)-TSAVLQSGFRK-2,4-dinitrophenyl amide (DNP); 11-mer peptide cleaved at the Q-S site |
| SARS-CoV main protease (3C-like protease)variant Δ(302–306) | severe acute respiratory syndrome coronavirus (SARS-CoV) | ortho-aminobenzoic acid (ABZ)-TSAVLQSGFRK-2,4-dinitrophenyl amide (DNP); 11-mer peptide cleaved at the Q-S site | 0.03 | 0.022 | 1.4 calc. | pH 7.6, 30 °C | PMID 18275836 | Open record: SARS-CoV main protease (3C-like protease) variant Δ(302–306), ortho-aminobenzoic acid (ABZ)-TSAVLQSGFRK-2,4-dinitrophenyl amide (DNP); 11-mer peptide cleaved at the Q-S site |
| SARS-CoV main protease (3C-like protease)variant Δ(303–306) | severe acute respiratory syndrome coronavirus (SARS-CoV) | ortho-aminobenzoic acid (ABZ)-TSAVLQSGFRK-2,4-dinitrophenyl amide (DNP); 11-mer peptide cleaved at the Q-S site | 0.022 | 0.004 | 5.5 calc. | pH 7.6, 30 °C | PMID 18275836 | Open record: SARS-CoV main protease (3C-like protease) variant Δ(303–306), ortho-aminobenzoic acid (ABZ)-TSAVLQSGFRK-2,4-dinitrophenyl amide (DNP); 11-mer peptide cleaved at the Q-S site |
| SARS-CoV main protease (3C-like protease)variant Δ(304–306) | severe acute respiratory syndrome coronavirus (SARS-CoV) | ortho-aminobenzoic acid (ABZ)-TSAVLQSGFRK-2,4-dinitrophenyl amide (DNP); 11-mer peptide cleaved at the Q-S site | 0.027 | 0.006 | 4.5 calc. | pH 7.6, 30 °C | PMID 18275836 | Open record: SARS-CoV main protease (3C-like protease) variant Δ(304–306), ortho-aminobenzoic acid (ABZ)-TSAVLQSGFRK-2,4-dinitrophenyl amide (DNP); 11-mer peptide cleaved at the Q-S site |
| SARS-CoV main protease (3C-like protease)variant Δ(305−306) | severe acute respiratory syndrome coronavirus (SARS-CoV) | ortho-aminobenzoic acid (ABZ)-TSAVLQSGFRK-2,4-dinitrophenyl amide (DNP); 11-mer peptide cleaved at the Q-S site | 0.039 | 0.014 | 2.8 calc. | pH 7.6, 30 °C | PMID 18275836 | Open record: SARS-CoV main protease (3C-like protease) variant Δ(305−306), ortho-aminobenzoic acid (ABZ)-TSAVLQSGFRK-2,4-dinitrophenyl amide (DNP); 11-mer peptide cleaved at the Q-S site |
| SARS-CoV main protease (3C-like protease)variant Δ(306) | severe acute respiratory syndrome coronavirus (SARS-CoV) | ortho-aminobenzoic acid (ABZ)-TSAVLQSGFRK-2,4-dinitrophenyl amide (DNP); 11-mer peptide cleaved at the Q-S site | 0.043 | 0.021 | 2.0 calc. | pH 7.6, 30 °C | PMID 18275836 | Open record: SARS-CoV main protease (3C-like protease) variant Δ(306), ortho-aminobenzoic acid (ABZ)-TSAVLQSGFRK-2,4-dinitrophenyl amide (DNP); 11-mer peptide cleaved at the Q-S site |
| SARS-CoV main protease (Mpro) | SARS-CoV | TSAVLQ-para-nitroanilidesubstrate-titration assay in 10 mM phosphate buffer | 0.63 | 0.2226 | 2.83 | pH 7.6, 30 °C | PMID 20371333 | Open record: SARS-CoV main protease (Mpro), TSAVLQ-para-nitroanilide |
| SARS-CoV main protease (Mpro) | SARS-CoV | TSAVLQ-para-nitroanilideenzyme-concentration dependence in 10 mM phosphate buffer | 1.4 | — | — | pH 7.6, 30 °C | PMID 20371333 | Open record: SARS-CoV main protease (Mpro), TSAVLQ-para-nitroanilide |
| SARS-CoV main protease (Mpro)variant E166A | SARS-CoV | TSAVLQ-para-nitroanilide | 0.31 | 0.3534 | 0.877 | pH 7.6, 30 °C | PMID 20371333 | Open record: SARS-CoV main protease (Mpro) variant E166A, TSAVLQ-para-nitroanilide |
| SARS-CoV main protease (Mpro)variant R298A | SARS-CoV | TSAVLQ-para-nitroanilidesubstrate-titration assay in 10 mM phosphate buffer | 0.1 | — | — | pH 7.6, 30 °C | PMID 20371333 | Open record: SARS-CoV main protease (Mpro) variant R298A, TSAVLQ-para-nitroanilide |
| SARS-CoV main protease (Mpro)variant R298A | SARS-CoV | TSAVLQ-para-nitroanilideenzyme-concentration dependence in 10 mM phosphate buffer | 0.7 | — | — | pH 7.6, 30 °C | PMID 20371333 | Open record: SARS-CoV main protease (Mpro) variant R298A, TSAVLQ-para-nitroanilide |
| SARS-CoV main protease (Mpro)variant R298L | SARS-CoV | TSAVLQ-para-nitroanilidesubstrate-titration assay in 10 mM phosphate buffer | 0.48 | — | — | pH 7.6, 30 °C | PMID 20371333 | Open record: SARS-CoV main protease (Mpro) variant R298L, TSAVLQ-para-nitroanilide |
| SARS-CoV main protease (Mpro)variant R298L | SARS-CoV | TSAVLQ-para-nitroanilideenzyme-concentration dependence in 10 mM phosphate buffer | 2.3 | — | — | pH 7.6, 30 °C | PMID 20371333 | Open record: SARS-CoV main protease (Mpro) variant R298L, TSAVLQ-para-nitroanilide |
| ubiquitin carboxy-terminal hydrolase L1 (UCH-L1) | — | ubiquitin C-terminal 7-amido-4-methylcoumarin (ubiquitin-AMC) | 0.02 | 4 × 10⁻⁵ | 510 | pH 7.6, 25 °C | PMID 17144664 | Open record: ubiquitin carboxy-terminal hydrolase L1 (UCH-L1), ubiquitin C-terminal 7-amido-4-methylcoumarin (ubiquitin-AMC) |
| ubiquitin carboxy-terminal hydrolase L1 (UCH-L1) | — | ubiquitin fused at the C-terminus to alanine-tryptophan | 1 × 10⁻⁴ | 8 × 10⁻⁵ | 1 | pH 7.6, 25 °C | PMID 17144664 | Open record: ubiquitin carboxy-terminal hydrolase L1 (UCH-L1), ubiquitin fused at the C-terminus to alanine-tryptophan |
| ubiquitin carboxy-terminal hydrolase L1 (UCH-L1) | — | ubiquitin fused at the C-terminus to tryptophan | 0.03 | 1.3 × 10⁻⁴ | 197 | pH 7.6, 25 °C | PMID 17144664 | Open record: ubiquitin carboxy-terminal hydrolase L1 (UCH-L1), ubiquitin fused at the C-terminus to tryptophan |
| ubiquitin carboxy-terminal hydrolase L1 (UCH-L1) | — | ubiquitin fused at the C-terminus to tryptophan-alanine | 5 × 10⁻⁴ | 4.5 × 10⁻⁴ | 1 | pH 7.6, 25 °C | PMID 17144664 | Open record: ubiquitin carboxy-terminal hydrolase L1 (UCH-L1), ubiquitin fused at the C-terminus to tryptophan-alanine |
| ubiquitin carboxy-terminal hydrolase L1 (UCH-L1) | — | ubiquitin G75A mutant fused at the C-terminus to tryptophan (UbW G75A variant) | 0.01 | 1 × 10⁻⁴ | 217 | pH 7.6, 25 °C | PMID 17144664 | Open record: ubiquitin carboxy-terminal hydrolase L1 (UCH-L1), ubiquitin G75A mutant fused at the C-terminus to tryptophan (UbW G75A variant) |
| ubiquitin carboxy-terminal hydrolase L1 (UCH-L1) | — | ubiquitin G76A mutant fused at the C-terminus to tryptophan (UbW G76A variant) | 0.0011 | 0.002 | 1 | pH 7.6, 25 °C | PMID 17144664 | Open record: ubiquitin carboxy-terminal hydrolase L1 (UCH-L1), ubiquitin G76A mutant fused at the C-terminus to tryptophan (UbW G76A variant) |
| ubiquitin carboxy-terminal hydrolase L1 (UCH-L1) | — | ubiquitin H68A mutant fused at the C-terminus to tryptophan (UbW H68A variant) | 0.06 | 5 × 10⁻⁴ | 100 | pH 7.6, 25 °C | PMID 17144664 | Open record: ubiquitin carboxy-terminal hydrolase L1 (UCH-L1), ubiquitin H68A mutant fused at the C-terminus to tryptophan (UbW H68A variant) |
| ubiquitin carboxy-terminal hydrolase L1 (UCH-L1) | — | ubiquitin I44A mutant fused at the C-terminus to tryptophan (UbW I44A variant) | 0.09 | 0.0031 | 28 | pH 7.6, 25 °C | PMID 17144664 | Open record: ubiquitin carboxy-terminal hydrolase L1 (UCH-L1), ubiquitin I44A mutant fused at the C-terminus to tryptophan (UbW I44A variant) |
| ubiquitin carboxy-terminal hydrolase L1 (UCH-L1) | — | ubiquitin K11A mutant fused at the C-terminus to tryptophan (UbW K11A variant) | 0.03 | 0.0011 | 27 | pH 7.6, 25 °C | PMID 17144664 | Open record: ubiquitin carboxy-terminal hydrolase L1 (UCH-L1), ubiquitin K11A mutant fused at the C-terminus to tryptophan (UbW K11A variant) |
| ubiquitin carboxy-terminal hydrolase L1 (UCH-L1) | — | ubiquitin K48A mutant fused at the C-terminus to tryptophan (UbW K48A variant) | 0.04 | 3 × 10⁻⁴ | 119 | pH 7.6, 25 °C | PMID 17144664 | Open record: ubiquitin carboxy-terminal hydrolase L1 (UCH-L1), ubiquitin K48A mutant fused at the C-terminus to tryptophan (UbW K48A variant) |
| ubiquitin carboxy-terminal hydrolase L1 (UCH-L1) | — | ubiquitin K63A mutant fused at the C-terminus to tryptophan (UbW K63A variant) | 0.03 | 4 × 10⁻⁴ | 67 | pH 7.6, 25 °C | PMID 17144664 | Open record: ubiquitin carboxy-terminal hydrolase L1 (UCH-L1), ubiquitin K63A mutant fused at the C-terminus to tryptophan (UbW K63A variant) |
| ubiquitin carboxy-terminal hydrolase L1 (UCH-L1) | — | ubiquitin K6A mutant fused at the C-terminus to tryptophan (UbW K6A variant) | 0.03 | 9 × 10⁻⁴ | 39 | pH 7.6, 25 °C | PMID 17144664 | Open record: ubiquitin carboxy-terminal hydrolase L1 (UCH-L1), ubiquitin K6A mutant fused at the C-terminus to tryptophan (UbW K6A variant) |
| ubiquitin carboxy-terminal hydrolase L1 (UCH-L1) | — | ubiquitin L71A mutant fused at the C-terminus to tryptophan (UbW L71A variant) | 0.03 | 0.008 | 3 | pH 7.6, 25 °C | PMID 17144664 | Open record: ubiquitin carboxy-terminal hydrolase L1 (UCH-L1), ubiquitin L71A mutant fused at the C-terminus to tryptophan (UbW L71A variant) |
| ubiquitin carboxy-terminal hydrolase L1 (UCH-L1) | — | ubiquitin L73A mutant fused at the C-terminus to tryptophan (UbW L73A variant) | 0.01 | 0.0058 | 2 | pH 7.6, 25 °C | PMID 17144664 | Open record: ubiquitin carboxy-terminal hydrolase L1 (UCH-L1), ubiquitin L73A mutant fused at the C-terminus to tryptophan (UbW L73A variant) |
| ubiquitin carboxy-terminal hydrolase L1 (UCH-L1) | — | ubiquitin L8A mutant fused at the C-terminus to tryptophan (UbW L8A variant) | — | — | 1 | pH 7.6, 25 °C | PMID 17144664 | Open record: ubiquitin carboxy-terminal hydrolase L1 (UCH-L1), ubiquitin L8A mutant fused at the C-terminus to tryptophan (UbW L8A variant) |
| ubiquitin carboxy-terminal hydrolase L1 (UCH-L1) | — | ubiquitin R42A mutant fused at the C-terminus to tryptophan (UbW R42A variant) | 0.02 | 1 × 10⁻⁴ | 308 | pH 7.6, 25 °C | PMID 17144664 | Open record: ubiquitin carboxy-terminal hydrolase L1 (UCH-L1), ubiquitin R42A mutant fused at the C-terminus to tryptophan (UbW R42A variant) |
| ubiquitin carboxy-terminal hydrolase L1 (UCH-L1) | — | ubiquitin R72A mutant fused at the C-terminus to tryptophan (UbW R72A variant) | 0.03 | 6 × 10⁻⁴ | 54 | pH 7.6, 25 °C | PMID 17144664 | Open record: ubiquitin carboxy-terminal hydrolase L1 (UCH-L1), ubiquitin R72A mutant fused at the C-terminus to tryptophan (UbW R72A variant) |
| ubiquitin carboxy-terminal hydrolase L1 (UCH-L1) | — | ubiquitin R74A mutant fused at the C-terminus to tryptophan (UbW R74A variant) | 0.01 | 2 × 10⁻⁴ | 63 | pH 7.6, 25 °C | PMID 17144664 | Open record: ubiquitin carboxy-terminal hydrolase L1 (UCH-L1), ubiquitin R74A mutant fused at the C-terminus to tryptophan (UbW R74A variant) |
| ubiquitin carboxy-terminal hydrolase L1 (UCH-L1) | — | ubiquitin V70A mutant fused at the C-terminus to tryptophan (UbW V70A variant) | 0.05 | 6 × 10⁻⁴ | 78 | pH 7.6, 25 °C | PMID 17144664 | Open record: ubiquitin carboxy-terminal hydrolase L1 (UCH-L1), ubiquitin V70A mutant fused at the C-terminus to tryptophan (UbW V70A variant) |
| ubiquitin carboxy-terminal hydrolase L3 (UCH-L3) | — | ubiquitin C-terminal 7-amido-4-methylcoumarin (ubiquitin-AMC) | 5.9 | 2 × 10⁻⁵ | 2.773 × 10⁵ | pH 7.6, 25 °C | PMID 17144664 | Open record: ubiquitin carboxy-terminal hydrolase L3 (UCH-L3), ubiquitin C-terminal 7-amido-4-methylcoumarin (ubiquitin-AMC) |
| ubiquitin carboxy-terminal hydrolase L3 (UCH-L3) | — | ubiquitin fused at the C-terminus to alanine-tryptophan | 1.5 | 1.6 × 10⁻⁴ | 9.49 × 10³ | pH 7.6, 25 °C | PMID 17144664 | Open record: ubiquitin carboxy-terminal hydrolase L3 (UCH-L3), ubiquitin fused at the C-terminus to alanine-tryptophan |
| ubiquitin carboxy-terminal hydrolase L3 (UCH-L3) | — | ubiquitin fused at the C-terminus to tryptophan | 2.6 | 2 × 10⁻⁴ | 1.301 × 10⁴ | pH 7.6, 25 °C | PMID 17144664 | Open record: ubiquitin carboxy-terminal hydrolase L3 (UCH-L3), ubiquitin fused at the C-terminus to tryptophan |
| ubiquitin carboxy-terminal hydrolase L3 (UCH-L3) | — | ubiquitin fused at the C-terminus to tryptophan-alanine | 2.4 | 7.8 × 10⁻⁴ | 3.04 × 10³ | pH 7.6, 25 °C | PMID 17144664 | Open record: ubiquitin carboxy-terminal hydrolase L3 (UCH-L3), ubiquitin fused at the C-terminus to tryptophan-alanine |
| ubiquitin carboxy-terminal hydrolase L3 (UCH-L3) | — | ubiquitin G75A mutant fused at the C-terminus to tryptophan (UbW G75A variant) | 2.9 | 4 × 10⁻⁴ | 7.27 × 10³ | pH 7.6, 25 °C | PMID 17144664 | Open record: ubiquitin carboxy-terminal hydrolase L3 (UCH-L3), ubiquitin G75A mutant fused at the C-terminus to tryptophan (UbW G75A variant) |
| ubiquitin carboxy-terminal hydrolase L3 (UCH-L3) | — | ubiquitin G76A mutant fused at the C-terminus to tryptophan (UbW G76A variant) | 1.7 | 0.0011 | 1594 | pH 7.6, 25 °C | PMID 17144664 | Open record: ubiquitin carboxy-terminal hydrolase L3 (UCH-L3), ubiquitin G76A mutant fused at the C-terminus to tryptophan (UbW G76A variant) |
| ubiquitin carboxy-terminal hydrolase L3 (UCH-L3) | — | ubiquitin H68A mutant fused at the C-terminus to tryptophan (UbW H68A variant) | 4.6 | 5 × 10⁻⁴ | 9.6 × 10³ | pH 7.6, 25 °C | PMID 17144664 | Open record: ubiquitin carboxy-terminal hydrolase L3 (UCH-L3), ubiquitin H68A mutant fused at the C-terminus to tryptophan (UbW H68A variant) |
| ubiquitin carboxy-terminal hydrolase L3 (UCH-L3) | — | ubiquitin I44A mutant fused at the C-terminus to tryptophan (UbW I44A variant) | 1.6 | 4 × 10⁻⁴ | 3.83 × 10³ | pH 7.6, 25 °C | PMID 17144664 | Open record: ubiquitin carboxy-terminal hydrolase L3 (UCH-L3), ubiquitin I44A mutant fused at the C-terminus to tryptophan (UbW I44A variant) |
| ubiquitin carboxy-terminal hydrolase L3 (UCH-L3) | — | ubiquitin K11A mutant fused at the C-terminus to tryptophan (UbW K11A variant) | 3.4 | 0.0023 | 1491 | pH 7.6, 25 °C | PMID 17144664 | Open record: ubiquitin carboxy-terminal hydrolase L3 (UCH-L3), ubiquitin K11A mutant fused at the C-terminus to tryptophan (UbW K11A variant) |
| ubiquitin carboxy-terminal hydrolase L3 (UCH-L3) | — | ubiquitin K48A mutant fused at the C-terminus to tryptophan (UbW K48A variant) | 2.4 | 7 × 10⁻⁴ | 3331 | pH 7.6, 25 °C | PMID 17144664 | Open record: ubiquitin carboxy-terminal hydrolase L3 (UCH-L3), ubiquitin K48A mutant fused at the C-terminus to tryptophan (UbW K48A variant) |
| ubiquitin carboxy-terminal hydrolase L3 (UCH-L3) | — | ubiquitin K63A mutant fused at the C-terminus to tryptophan (UbW K63A variant) | 2.4 | 8 × 10⁻⁴ | 3.23 × 10³ | pH 7.6, 25 °C | PMID 17144664 | Open record: ubiquitin carboxy-terminal hydrolase L3 (UCH-L3), ubiquitin K63A mutant fused at the C-terminus to tryptophan (UbW K63A variant) |
| ubiquitin carboxy-terminal hydrolase L3 (UCH-L3) | — | ubiquitin K6A mutant fused at the C-terminus to tryptophan (UbW K6A variant) | 4 | 0.0014 | 2.75 × 10³ | pH 7.6, 25 °C | PMID 17144664 | Open record: ubiquitin carboxy-terminal hydrolase L3 (UCH-L3), ubiquitin K6A mutant fused at the C-terminus to tryptophan (UbW K6A variant) |
| ubiquitin carboxy-terminal hydrolase L3 (UCH-L3) | — | ubiquitin L71A mutant fused at the C-terminus to tryptophan (UbW L71A variant) | 9.2 | 0.0198 | 462 | pH 7.6, 25 °C | PMID 17144664 | Open record: ubiquitin carboxy-terminal hydrolase L3 (UCH-L3), ubiquitin L71A mutant fused at the C-terminus to tryptophan (UbW L71A variant) |
| ubiquitin carboxy-terminal hydrolase L3 (UCH-L3) | — | ubiquitin L73A mutant fused at the C-terminus to tryptophan (UbW L73A variant) | 5.7 | 0.0104 | 547 | pH 7.6, 25 °C | PMID 17144664 | Open record: ubiquitin carboxy-terminal hydrolase L3 (UCH-L3), ubiquitin L73A mutant fused at the C-terminus to tryptophan (UbW L73A variant) |
| ubiquitin carboxy-terminal hydrolase L3 (UCH-L3) | — | ubiquitin L8A mutant fused at the C-terminus to tryptophan (UbW L8A variant) | 6.6 | 0.0282 | 236 | pH 7.6, 25 °C | PMID 17144664 | Open record: ubiquitin carboxy-terminal hydrolase L3 (UCH-L3), ubiquitin L8A mutant fused at the C-terminus to tryptophan (UbW L8A variant) |
| ubiquitin carboxy-terminal hydrolase L3 (UCH-L3) | — | ubiquitin R42A mutant fused at the C-terminus to tryptophan (UbW R42A variant) | 0.2 | 1 × 10⁻⁴ | 4991 | pH 7.6, 25 °C | PMID 17144664 | Open record: ubiquitin carboxy-terminal hydrolase L3 (UCH-L3), ubiquitin R42A mutant fused at the C-terminus to tryptophan (UbW R42A variant) |
| ubiquitin carboxy-terminal hydrolase L3 (UCH-L3) | — | ubiquitin R72A mutant fused at the C-terminus to tryptophan (UbW R72A variant) | 4.4 | 0.0019 | 2.27 × 10³ | pH 7.6, 25 °C | PMID 17144664 | Open record: ubiquitin carboxy-terminal hydrolase L3 (UCH-L3), ubiquitin R72A mutant fused at the C-terminus to tryptophan (UbW R72A variant) |
| ubiquitin carboxy-terminal hydrolase L3 (UCH-L3) | — | ubiquitin R74A mutant fused at the C-terminus to tryptophan (UbW R74A variant) | 0.3 | 0.0045 | 65 | pH 7.6, 25 °C | PMID 17144664 | Open record: ubiquitin carboxy-terminal hydrolase L3 (UCH-L3), ubiquitin R74A mutant fused at the C-terminus to tryptophan (UbW R74A variant) |
| ubiquitin carboxy-terminal hydrolase L3 (UCH-L3) | — | ubiquitin V70A mutant fused at the C-terminus to tryptophan (UbW V70A variant) | 6.7 | 0.0011 | 6.36 × 10³ | pH 7.6, 25 °C | PMID 17144664 | Open record: ubiquitin carboxy-terminal hydrolase L3 (UCH-L3), ubiquitin V70A mutant fused at the C-terminus to tryptophan (UbW V70A variant) |
| ubiquitin-specific protease 15 (USP15) | Homo sapiens | ubiquitin-7-amido-4-methylcoumarin | 0.149 | 2.34 × 10⁻⁴ | 646.2 | pH 7.5, 25 °C | PMID 42259887 | Open record: ubiquitin-specific protease 15 (USP15), ubiquitin-7-amido-4-methylcoumarin |
| ubiquitin-specific protease 15 (USP15)variant P331S | Homo sapiens | ubiquitin-7-amido-4-methylcoumarin | 0.077 | 4.59 × 10⁻⁴ | 170.9 | pH 7.5, 25 °C | PMID 42259887 | Open record: ubiquitin-specific protease 15 (USP15) variant P331S, ubiquitin-7-amido-4-methylcoumarin |