Values CatLog read from this paper
Substrate recognition and catalysis by UCH-L1
PMID 17144664doi:10.1021/bi061406cOpen in Browse
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CatLog read 38 records (112 values) from this paper: ubiquitin carboxy-terminal hydrolase L1 (UCH-L1), ubiquitin carboxy-terminal hydrolase L3 (UCH-L3).
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| ubiquitin carboxy-terminal hydrolase L1 (UCH-L1) | — | ubiquitin C-terminal 7-amido-4-methylcoumarin (ubiquitin-AMC) | 0.02 | 4 × 10⁻⁵ | 510 | pH 7.6, 25 °C | Open record: ubiquitin carboxy-terminal hydrolase L1 (UCH-L1), ubiquitin C-terminal 7-amido-4-methylcoumarin (ubiquitin-AMC) |
| ubiquitin carboxy-terminal hydrolase L1 (UCH-L1) | — | ubiquitin fused at the C-terminus to alanine-tryptophan | 1 × 10⁻⁴ | 8 × 10⁻⁵ | 1 | pH 7.6, 25 °C | Open record: ubiquitin carboxy-terminal hydrolase L1 (UCH-L1), ubiquitin fused at the C-terminus to alanine-tryptophan |
| ubiquitin carboxy-terminal hydrolase L1 (UCH-L1) | — | ubiquitin fused at the C-terminus to tryptophan | 0.03 | 1.3 × 10⁻⁴ | 197 | pH 7.6, 25 °C | Open record: ubiquitin carboxy-terminal hydrolase L1 (UCH-L1), ubiquitin fused at the C-terminus to tryptophan |
| ubiquitin carboxy-terminal hydrolase L1 (UCH-L1) | — | ubiquitin fused at the C-terminus to tryptophan-alanine | 5 × 10⁻⁴ | 4.5 × 10⁻⁴ | 1 | pH 7.6, 25 °C | Open record: ubiquitin carboxy-terminal hydrolase L1 (UCH-L1), ubiquitin fused at the C-terminus to tryptophan-alanine |
| ubiquitin carboxy-terminal hydrolase L1 (UCH-L1) | — | ubiquitin G75A mutant fused at the C-terminus to tryptophan (UbW G75A variant) | 0.01 | 1 × 10⁻⁴ | 217 | pH 7.6, 25 °C | Open record: ubiquitin carboxy-terminal hydrolase L1 (UCH-L1), ubiquitin G75A mutant fused at the C-terminus to tryptophan (UbW G75A variant) |
| ubiquitin carboxy-terminal hydrolase L1 (UCH-L1) | — | ubiquitin G76A mutant fused at the C-terminus to tryptophan (UbW G76A variant) | 0.0011 | 0.002 | 1 | pH 7.6, 25 °C | Open record: ubiquitin carboxy-terminal hydrolase L1 (UCH-L1), ubiquitin G76A mutant fused at the C-terminus to tryptophan (UbW G76A variant) |
| ubiquitin carboxy-terminal hydrolase L1 (UCH-L1) | — | ubiquitin H68A mutant fused at the C-terminus to tryptophan (UbW H68A variant) | 0.06 | 5 × 10⁻⁴ | 100 | pH 7.6, 25 °C | Open record: ubiquitin carboxy-terminal hydrolase L1 (UCH-L1), ubiquitin H68A mutant fused at the C-terminus to tryptophan (UbW H68A variant) |
| ubiquitin carboxy-terminal hydrolase L1 (UCH-L1) | — | ubiquitin I44A mutant fused at the C-terminus to tryptophan (UbW I44A variant) | 0.09 | 0.0031 | 28 | pH 7.6, 25 °C | Open record: ubiquitin carboxy-terminal hydrolase L1 (UCH-L1), ubiquitin I44A mutant fused at the C-terminus to tryptophan (UbW I44A variant) |
| ubiquitin carboxy-terminal hydrolase L1 (UCH-L1) | — | ubiquitin K11A mutant fused at the C-terminus to tryptophan (UbW K11A variant) | 0.03 | 0.0011 | 27 | pH 7.6, 25 °C | Open record: ubiquitin carboxy-terminal hydrolase L1 (UCH-L1), ubiquitin K11A mutant fused at the C-terminus to tryptophan (UbW K11A variant) |
| ubiquitin carboxy-terminal hydrolase L1 (UCH-L1) | — | ubiquitin K48A mutant fused at the C-terminus to tryptophan (UbW K48A variant) | 0.04 | 3 × 10⁻⁴ | 119 | pH 7.6, 25 °C | Open record: ubiquitin carboxy-terminal hydrolase L1 (UCH-L1), ubiquitin K48A mutant fused at the C-terminus to tryptophan (UbW K48A variant) |
| ubiquitin carboxy-terminal hydrolase L1 (UCH-L1) | — | ubiquitin K63A mutant fused at the C-terminus to tryptophan (UbW K63A variant) | 0.03 | 4 × 10⁻⁴ | 67 | pH 7.6, 25 °C | Open record: ubiquitin carboxy-terminal hydrolase L1 (UCH-L1), ubiquitin K63A mutant fused at the C-terminus to tryptophan (UbW K63A variant) |
| ubiquitin carboxy-terminal hydrolase L1 (UCH-L1) | — | ubiquitin K6A mutant fused at the C-terminus to tryptophan (UbW K6A variant) | 0.03 | 9 × 10⁻⁴ | 39 | pH 7.6, 25 °C | Open record: ubiquitin carboxy-terminal hydrolase L1 (UCH-L1), ubiquitin K6A mutant fused at the C-terminus to tryptophan (UbW K6A variant) |
| ubiquitin carboxy-terminal hydrolase L1 (UCH-L1) | — | ubiquitin L71A mutant fused at the C-terminus to tryptophan (UbW L71A variant) | 0.03 | 0.008 | 3 | pH 7.6, 25 °C | Open record: ubiquitin carboxy-terminal hydrolase L1 (UCH-L1), ubiquitin L71A mutant fused at the C-terminus to tryptophan (UbW L71A variant) |
| ubiquitin carboxy-terminal hydrolase L1 (UCH-L1) | — | ubiquitin L73A mutant fused at the C-terminus to tryptophan (UbW L73A variant) | 0.01 | 0.0058 | 2 | pH 7.6, 25 °C | Open record: ubiquitin carboxy-terminal hydrolase L1 (UCH-L1), ubiquitin L73A mutant fused at the C-terminus to tryptophan (UbW L73A variant) |
| ubiquitin carboxy-terminal hydrolase L1 (UCH-L1) | — | ubiquitin L8A mutant fused at the C-terminus to tryptophan (UbW L8A variant) | — | — | 1 | pH 7.6, 25 °C | Open record: ubiquitin carboxy-terminal hydrolase L1 (UCH-L1), ubiquitin L8A mutant fused at the C-terminus to tryptophan (UbW L8A variant) |
| ubiquitin carboxy-terminal hydrolase L1 (UCH-L1) | — | ubiquitin R42A mutant fused at the C-terminus to tryptophan (UbW R42A variant) | 0.02 | 1 × 10⁻⁴ | 308 | pH 7.6, 25 °C | Open record: ubiquitin carboxy-terminal hydrolase L1 (UCH-L1), ubiquitin R42A mutant fused at the C-terminus to tryptophan (UbW R42A variant) |
| ubiquitin carboxy-terminal hydrolase L1 (UCH-L1) | — | ubiquitin R72A mutant fused at the C-terminus to tryptophan (UbW R72A variant) | 0.03 | 6 × 10⁻⁴ | 54 | pH 7.6, 25 °C | Open record: ubiquitin carboxy-terminal hydrolase L1 (UCH-L1), ubiquitin R72A mutant fused at the C-terminus to tryptophan (UbW R72A variant) |
| ubiquitin carboxy-terminal hydrolase L1 (UCH-L1) | — | ubiquitin R74A mutant fused at the C-terminus to tryptophan (UbW R74A variant) | 0.01 | 2 × 10⁻⁴ | 63 | pH 7.6, 25 °C | Open record: ubiquitin carboxy-terminal hydrolase L1 (UCH-L1), ubiquitin R74A mutant fused at the C-terminus to tryptophan (UbW R74A variant) |
| ubiquitin carboxy-terminal hydrolase L1 (UCH-L1) | — | ubiquitin V70A mutant fused at the C-terminus to tryptophan (UbW V70A variant) | 0.05 | 6 × 10⁻⁴ | 78 | pH 7.6, 25 °C | Open record: ubiquitin carboxy-terminal hydrolase L1 (UCH-L1), ubiquitin V70A mutant fused at the C-terminus to tryptophan (UbW V70A variant) |
| ubiquitin carboxy-terminal hydrolase L3 (UCH-L3) | — | ubiquitin C-terminal 7-amido-4-methylcoumarin (ubiquitin-AMC) | 5.9 | 2 × 10⁻⁵ | 2.773 × 10⁵ | pH 7.6, 25 °C | Open record: ubiquitin carboxy-terminal hydrolase L3 (UCH-L3), ubiquitin C-terminal 7-amido-4-methylcoumarin (ubiquitin-AMC) |
| ubiquitin carboxy-terminal hydrolase L3 (UCH-L3) | — | ubiquitin fused at the C-terminus to alanine-tryptophan | 1.5 | 1.6 × 10⁻⁴ | 9.49 × 10³ | pH 7.6, 25 °C | Open record: ubiquitin carboxy-terminal hydrolase L3 (UCH-L3), ubiquitin fused at the C-terminus to alanine-tryptophan |
| ubiquitin carboxy-terminal hydrolase L3 (UCH-L3) | — | ubiquitin fused at the C-terminus to tryptophan | 2.6 | 2 × 10⁻⁴ | 1.301 × 10⁴ | pH 7.6, 25 °C | Open record: ubiquitin carboxy-terminal hydrolase L3 (UCH-L3), ubiquitin fused at the C-terminus to tryptophan |
| ubiquitin carboxy-terminal hydrolase L3 (UCH-L3) | — | ubiquitin fused at the C-terminus to tryptophan-alanine | 2.4 | 7.8 × 10⁻⁴ | 3.04 × 10³ | pH 7.6, 25 °C | Open record: ubiquitin carboxy-terminal hydrolase L3 (UCH-L3), ubiquitin fused at the C-terminus to tryptophan-alanine |
| ubiquitin carboxy-terminal hydrolase L3 (UCH-L3) | — | ubiquitin G75A mutant fused at the C-terminus to tryptophan (UbW G75A variant) | 2.9 | 4 × 10⁻⁴ | 7.27 × 10³ | pH 7.6, 25 °C | Open record: ubiquitin carboxy-terminal hydrolase L3 (UCH-L3), ubiquitin G75A mutant fused at the C-terminus to tryptophan (UbW G75A variant) |
| ubiquitin carboxy-terminal hydrolase L3 (UCH-L3) | — | ubiquitin G76A mutant fused at the C-terminus to tryptophan (UbW G76A variant) | 1.7 | 0.0011 | 1594 | pH 7.6, 25 °C | Open record: ubiquitin carboxy-terminal hydrolase L3 (UCH-L3), ubiquitin G76A mutant fused at the C-terminus to tryptophan (UbW G76A variant) |
| ubiquitin carboxy-terminal hydrolase L3 (UCH-L3) | — | ubiquitin H68A mutant fused at the C-terminus to tryptophan (UbW H68A variant) | 4.6 | 5 × 10⁻⁴ | 9.6 × 10³ | pH 7.6, 25 °C | Open record: ubiquitin carboxy-terminal hydrolase L3 (UCH-L3), ubiquitin H68A mutant fused at the C-terminus to tryptophan (UbW H68A variant) |
| ubiquitin carboxy-terminal hydrolase L3 (UCH-L3) | — | ubiquitin I44A mutant fused at the C-terminus to tryptophan (UbW I44A variant) | 1.6 | 4 × 10⁻⁴ | 3.83 × 10³ | pH 7.6, 25 °C | Open record: ubiquitin carboxy-terminal hydrolase L3 (UCH-L3), ubiquitin I44A mutant fused at the C-terminus to tryptophan (UbW I44A variant) |
| ubiquitin carboxy-terminal hydrolase L3 (UCH-L3) | — | ubiquitin K11A mutant fused at the C-terminus to tryptophan (UbW K11A variant) | 3.4 | 0.0023 | 1491 | pH 7.6, 25 °C | Open record: ubiquitin carboxy-terminal hydrolase L3 (UCH-L3), ubiquitin K11A mutant fused at the C-terminus to tryptophan (UbW K11A variant) |
| ubiquitin carboxy-terminal hydrolase L3 (UCH-L3) | — | ubiquitin K48A mutant fused at the C-terminus to tryptophan (UbW K48A variant) | 2.4 | 7 × 10⁻⁴ | 3331 | pH 7.6, 25 °C | Open record: ubiquitin carboxy-terminal hydrolase L3 (UCH-L3), ubiquitin K48A mutant fused at the C-terminus to tryptophan (UbW K48A variant) |
| ubiquitin carboxy-terminal hydrolase L3 (UCH-L3) | — | ubiquitin K63A mutant fused at the C-terminus to tryptophan (UbW K63A variant) | 2.4 | 8 × 10⁻⁴ | 3.23 × 10³ | pH 7.6, 25 °C | Open record: ubiquitin carboxy-terminal hydrolase L3 (UCH-L3), ubiquitin K63A mutant fused at the C-terminus to tryptophan (UbW K63A variant) |
| ubiquitin carboxy-terminal hydrolase L3 (UCH-L3) | — | ubiquitin K6A mutant fused at the C-terminus to tryptophan (UbW K6A variant) | 4 | 0.0014 | 2.75 × 10³ | pH 7.6, 25 °C | Open record: ubiquitin carboxy-terminal hydrolase L3 (UCH-L3), ubiquitin K6A mutant fused at the C-terminus to tryptophan (UbW K6A variant) |
| ubiquitin carboxy-terminal hydrolase L3 (UCH-L3) | — | ubiquitin L71A mutant fused at the C-terminus to tryptophan (UbW L71A variant) | 9.2 | 0.0198 | 462 | pH 7.6, 25 °C | Open record: ubiquitin carboxy-terminal hydrolase L3 (UCH-L3), ubiquitin L71A mutant fused at the C-terminus to tryptophan (UbW L71A variant) |
| ubiquitin carboxy-terminal hydrolase L3 (UCH-L3) | — | ubiquitin L73A mutant fused at the C-terminus to tryptophan (UbW L73A variant) | 5.7 | 0.0104 | 547 | pH 7.6, 25 °C | Open record: ubiquitin carboxy-terminal hydrolase L3 (UCH-L3), ubiquitin L73A mutant fused at the C-terminus to tryptophan (UbW L73A variant) |
| ubiquitin carboxy-terminal hydrolase L3 (UCH-L3) | — | ubiquitin L8A mutant fused at the C-terminus to tryptophan (UbW L8A variant) | 6.6 | 0.0282 | 236 | pH 7.6, 25 °C | Open record: ubiquitin carboxy-terminal hydrolase L3 (UCH-L3), ubiquitin L8A mutant fused at the C-terminus to tryptophan (UbW L8A variant) |
| ubiquitin carboxy-terminal hydrolase L3 (UCH-L3) | — | ubiquitin R42A mutant fused at the C-terminus to tryptophan (UbW R42A variant) | 0.2 | 1 × 10⁻⁴ | 4991 | pH 7.6, 25 °C | Open record: ubiquitin carboxy-terminal hydrolase L3 (UCH-L3), ubiquitin R42A mutant fused at the C-terminus to tryptophan (UbW R42A variant) |
| ubiquitin carboxy-terminal hydrolase L3 (UCH-L3) | — | ubiquitin R72A mutant fused at the C-terminus to tryptophan (UbW R72A variant) | 4.4 | 0.0019 | 2.27 × 10³ | pH 7.6, 25 °C | Open record: ubiquitin carboxy-terminal hydrolase L3 (UCH-L3), ubiquitin R72A mutant fused at the C-terminus to tryptophan (UbW R72A variant) |
| ubiquitin carboxy-terminal hydrolase L3 (UCH-L3) | — | ubiquitin R74A mutant fused at the C-terminus to tryptophan (UbW R74A variant) | 0.3 | 0.0045 | 65 | pH 7.6, 25 °C | Open record: ubiquitin carboxy-terminal hydrolase L3 (UCH-L3), ubiquitin R74A mutant fused at the C-terminus to tryptophan (UbW R74A variant) |
| ubiquitin carboxy-terminal hydrolase L3 (UCH-L3) | — | ubiquitin V70A mutant fused at the C-terminus to tryptophan (UbW V70A variant) | 6.7 | 0.0011 | 6.36 × 10³ | pH 7.6, 25 °C | Open record: ubiquitin carboxy-terminal hydrolase L3 (UCH-L3), ubiquitin V70A mutant fused at the C-terminus to tryptophan (UbW V70A variant) |