Values CatLog read from this paper
Mutation of Glu-166 blocks the substrate-induced dimerization of SARS coronavirus main protease
PMID 20371333doi:10.1016/j.bpj.2009.12.4272Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 7 records (11 values) from this paper: SARS-CoV main protease (Mpro) from SARS-CoV.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
EC 2.7.7.50, EC 3.4.19.12, EC 3.4.22.69
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| SARS-CoV main protease (Mpro) | SARS-CoV | TSAVLQ-para-nitroanilidesubstrate-titration assay in 10 mM phosphate buffer | 0.63 | 0.2226 | 2.83 | pH 7.6, 30 °C | Open record: SARS-CoV main protease (Mpro), TSAVLQ-para-nitroanilide |
| SARS-CoV main protease (Mpro) | SARS-CoV | TSAVLQ-para-nitroanilideenzyme-concentration dependence in 10 mM phosphate buffer | 1.4 | — | — | pH 7.6, 30 °C | Open record: SARS-CoV main protease (Mpro), TSAVLQ-para-nitroanilide |
| SARS-CoV main protease (Mpro)variant E166A | SARS-CoV | TSAVLQ-para-nitroanilide | 0.31 | 0.3534 | 0.877 | pH 7.6, 30 °C | Open record: SARS-CoV main protease (Mpro) variant E166A, TSAVLQ-para-nitroanilide |
| SARS-CoV main protease (Mpro)variant R298A | SARS-CoV | TSAVLQ-para-nitroanilidesubstrate-titration assay in 10 mM phosphate buffer | 0.1 | — | — | pH 7.6, 30 °C | Open record: SARS-CoV main protease (Mpro) variant R298A, TSAVLQ-para-nitroanilide |
| SARS-CoV main protease (Mpro)variant R298A | SARS-CoV | TSAVLQ-para-nitroanilideenzyme-concentration dependence in 10 mM phosphate buffer | 0.7 | — | — | pH 7.6, 30 °C | Open record: SARS-CoV main protease (Mpro) variant R298A, TSAVLQ-para-nitroanilide |
| SARS-CoV main protease (Mpro)variant R298L | SARS-CoV | TSAVLQ-para-nitroanilidesubstrate-titration assay in 10 mM phosphate buffer | 0.48 | — | — | pH 7.6, 30 °C | Open record: SARS-CoV main protease (Mpro) variant R298L, TSAVLQ-para-nitroanilide |
| SARS-CoV main protease (Mpro)variant R298L | SARS-CoV | TSAVLQ-para-nitroanilideenzyme-concentration dependence in 10 mM phosphate buffer | 2.3 | — | — | pH 7.6, 30 °C | Open record: SARS-CoV main protease (Mpro) variant R298L, TSAVLQ-para-nitroanilide |