CatLogenzyme kinetics catalog

Values CatLog read from this paper

Trypanosome mRNA recapping is triggered by hypermethylation originating from cap 4

PMID 39011881doi:10.1093/nar/gkae614Open in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 16 records (16 values) from this paper: TbCe1 (cytoplasmic capping enzyme, RNA kinase activity), TbCmt1 cap (guanine N7) methyltransferase from Trypanosoma brucei.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.

EC 2.7.7.50

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)Conditions
TbCe1 (cytoplasmic capping enzyme, RNA kinase activity)Trypanosoma brucei21-mer 5'-monophosphate RNA 5'-AACUAACGCUAUUAUUAGAAC-3' (first 21 nt of T. brucei SL RNA) with 2'-O-methyladenosine at A17.7 × 10⁻⁴——pH 8.5, 27 °COpen
TbCe1 (cytoplasmic capping enzyme, RNA kinase activity)Trypanosoma brucei21-mer 5'-monophosphate RNA 5'-AACUAACGCUAUUAUUAGAAC-3' (first 21 nt of T. brucei SL RNA) with 2'-O-ribose methylations on A1, A2, C3 and U49.5 × 10⁻⁴——pH 8.5, 27 °COpen
TbCe1 (cytoplasmic capping enzyme, RNA kinase activity)Trypanosoma brucei21-mer 5'-monophosphate RNA 5'-AACUAACGCUAUUAUUAGAAC-3' (first 21 nt of T. brucei SL RNA) with N1-methyladenosine at A11.2 × 10⁻⁴——pH 8.5, 27 °COpen
TbCe1 (cytoplasmic capping enzyme, RNA kinase activity)Trypanosoma brucei21-mer 5'-monophosphate RNA 5'-AACUAACGCUAUUAUUAGAAC-3' (first 21 nt of T. brucei SL RNA) with N6,N6,2'-O-trimethyladenosine at A10.03072——pH 8.5, 27 °COpen
TbCe1 (cytoplasmic capping enzyme, RNA kinase activity)Trypanosoma brucei21-mer 5'-monophosphate RNA 5'-AACUAACGCUAUUAUUAGAAC-3' (first 21 nt of T. brucei SL RNA) with N6,N6,2'-O-trimethyladenosine at A1 and 2'-O-ribose methylations on A2, C3 and U40.0264——pH 8.5, 27 °COpen
TbCe1 (cytoplasmic capping enzyme, RNA kinase activity)Trypanosoma brucei21-mer 5'-monophosphate RNA 5'-AACUAACGCUAUUAUUAGAAC-3' (first 21 nt of T. brucei SL RNA) with N6,N6,2'-O-trimethyladenosine at A1, 2'-O-methyl A2 and C3, N3,2'-O-dimethyluridine at U4 (decapped cap 4)0.038——pH 8.5, 27 °COpen
TbCe1 (cytoplasmic capping enzyme, RNA kinase activity)Trypanosoma brucei21-mer 5'-monophosphate RNA 5'-AACUAACGCUAUUAUUAGAAC-3' (first 21 nt of T. brucei SL RNA) with N6,N6-dimethyladenosine at A10.00353——pH 8.5, 27 °COpen
TbCe1 (cytoplasmic capping enzyme, RNA kinase activity)Trypanosoma brucei21-mer 5'-monophosphate RNA 5'-AACUAACGCUAUUAUUAGAAC-3' (first 21 nt of T. brucei SL RNA) with N6-methyladenosine at A10.00145——pH 8.5, 27 °COpen
TbCe1 (cytoplasmic capping enzyme, RNA kinase activity)Trypanosoma brucei21-mer 5'-monophosphate RNA 5'-AACUAACGCUAUUAUUAGAAC-3' (first 21 nt of T. brucei SL RNA) with N6-methyladenosine at A1 and 2'-O-ribose methylations on C3 and U40.00127——pH 8.5, 27 °COpen
TbCe1 (cytoplasmic capping enzyme, RNA kinase activity)Trypanosoma brucei21-mer 5'-monophosphate RNA 5'-AACUAACGCUAUUAUUAGAAC-3' (first 21 nt of T. brucei SL RNA), unmodified2.2 × 10⁻⁴——pH 8.5, 27 °COpen
TbCe1 (cytoplasmic capping enzyme, RNA kinase activity)Trypanosoma bruceiadenosine 5'-triphosphatep^{m6,2}A_mA_mC_m^{m3}U_m RNA—2.1 × 10⁻⁴—pH 8.5, 27 °COpen
TbCe1 (cytoplasmic capping enzyme, RNA kinase activity)Trypanosoma bruceiadenosine 5'-triphosphatepA_mA_mC_mU_m RNA—0.00209—pH 8.5, 27 °COpen
TbCe1 (cytoplasmic capping enzyme, RNA kinase activity)Trypanosoma bruceiadenosine 5'-triphosphatepA_m RNA—0.00325—pH 8.5, 27 °COpen
TbCe1 (cytoplasmic capping enzyme, RNA kinase activity)Trypanosoma bruceiadenosine 5'-triphosphateunmodified pRNA—0.00532—pH 8.5, 27 °COpen
TbCmt1 cap (guanine N7) methyltransferaseTrypanosoma bruceiguanylated (GpppRNA-capped) 21-mer SL RNA with 2'-O-ribose methylations on A1, A2, C3 and U40.098——pH 8, 27 °COpen
TbCmt1 cap (guanine N7) methyltransferaseTrypanosoma bruceiguanylated (GpppRNA-capped) 21-mer SL RNA without modification0.028——pH 8, 27 °COpen