EC 3.6.4.12: DNA helicase

HydrolasesOpen in Browse

CatLog read 14 records for EC 3.6.4.12 from 2 papers, for 2 organisms. Names in these papers: nsp13 helicase, RNA-dependent RNA polymerase (nsp12/nsp8 complex).

EC 3.6.4.12 is no longer current; BRENDA: “transferred to EC 5.6.2.3 and EC 5.6.2.4”.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units.

CatLog also holds 8 records for EC 3.6.4.12 that are not yet re-read: from its earlier version, built from the enzyme databases and partly checked against the papers. All records for EC 3.6.4.12 in Browse.

Papers

Papers with records for EC 3.6.4.12
Paper	Organisms	Records
Motif V is an allosteric couple between the SARS-CoV-2 nsp13 nucleotide triphosphatase and helicase active sites	SARS-CoV-2	12
Remdesivir is a direct-acting antiviral that inhibits RNA-dependent RNA polymerase from severe acute respiratory syndrome coronavirus 2 with high potency	Severe acute respiratory syndrome coronavirus	2

Records

Records for EC 3.6.4.12 read from the paper
Enzyme	Organism	Substrate	k_cat (s⁻¹)	K_m (mM)	k_cat/K_m (mM⁻¹ s⁻¹)	Conditions	Paper	Open record
nsp13 helicase	SARS-CoV-2	ATP100 nM labeled dsDNA	1.4	0.122	13	pH 7.5, 37 °C	PMID 41581884	Open record: nsp13 helicase, ATP
nsp13 helicase	SARS-CoV-2	ATPunlabeled dsDNA present	0.37	0.156	2.7	pH 7.5, 37 °C	PMID 41581884	Open record: nsp13 helicase, ATP
nsp13 helicase	SARS-CoV-2	dsDNA	1.5	2.85 × 10⁻⁴	6 × 10³	pH 7.5, 37 °C	PMID 41581884	Open record: nsp13 helicase, dsDNA
nsp13 helicasevariant D534A	SARS-CoV-2	ATP	0.26	0.135	1.9	pH 7.5, 37 °C	PMID 41581884	Open record: nsp13 helicase variant D534A, ATP
nsp13 helicasevariant L405D	SARS-CoV-2	ATP	0.9	0.118	10	pH 7.5, 37 °C	PMID 41581884	Open record: nsp13 helicase variant L405D, ATP
nsp13 helicasevariant L405D	SARS-CoV-2	dsDNA	10	—	3 × 10³	pH 7.5, 37 °C	PMID 41581884	Open record: nsp13 helicase variant L405D, dsDNA
nsp13 helicasevariant S535A	SARS-CoV-2	ATP100 nM labeled dsDNA	2.8	0.094	30	pH 7.5, 37 °C	PMID 41581884	Open record: nsp13 helicase variant S535A, ATP
nsp13 helicasevariant S535A	SARS-CoV-2	ATPunlabeled dsDNA present	0.47	0.176	2.7	pH 7.5, 37 °C	PMID 41581884	Open record: nsp13 helicase variant S535A, ATP
nsp13 helicasevariant S535A	SARS-CoV-2	dsDNA	2.4	1.75 × 10⁻⁴	1.6 × 10⁴	pH 7.5, 37 °C	PMID 41581884	Open record: nsp13 helicase variant S535A, dsDNA
nsp13 helicasevariant T532A	SARS-CoV-2	ATP100 nM labeled dsDNA	2.9	0.121	24	pH 7.5, 37 °C	PMID 41581884	Open record: nsp13 helicase variant T532A, ATP
nsp13 helicasevariant T532A	SARS-CoV-2	ATPunlabeled dsDNA present	0.61	0.126	5	pH 7.5, 37 °C	PMID 41581884	Open record: nsp13 helicase variant T532A, ATP
nsp13 helicasevariant T532A	SARS-CoV-2	dsDNA	3.7	2.82 × 10⁻⁴	1.4 × 10⁴	pH 7.5, 37 °C	PMID 41581884	Open record: nsp13 helicase variant T532A, dsDNA
RNA-dependent RNA polymerase (nsp12/nsp8 complex)	Severe acute respiratory syndrome coronavirus	adenosine 5'-triphosphate	—	3 × 10⁻⁵	—	pH 8, 30 °C	PMID 32284326	Open record: RNA-dependent RNA polymerase (nsp12/nsp8 complex), adenosine 5'-triphosphate
RNA-dependent RNA polymerase (nsp12/nsp8 complex)	Severe acute respiratory syndrome coronavirus	remdesivir triphosphate	—	1 × 10⁻⁵	—	pH 8, 30 °C	PMID 32284326	Open record: RNA-dependent RNA polymerase (nsp12/nsp8 complex), remdesivir triphosphate