EC 3.4.22.29: picornain 2A

CatLog read 14 records for EC 3.4.22.29 from 1 paper, for 1 organism. Names in these papers: HRV 3C protease.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units.

CatLog also holds 7 records for EC 3.4.22.29 that are not yet re-read: from its earlier version, built from the enzyme databases and partly checked against the papers. All records for EC 3.4.22.29 in Browse.

Papers

Papers with records for EC 3.4.22.29
Paper	Organisms	Records
A combinatorial strategy for HRV 3C protease engineering to achieve the N-terminal free cleavage	Human rhinovirus	14

Records

Records for EC 3.4.22.29 read from the paper
Enzyme	Organism	Substrate	k_cat (s⁻¹)	K_m (mM)	k_cat/K_m (mM⁻¹ s⁻¹)	Conditions	Paper	Open record
HRV 3C protease	Human rhinovirus	MBP-LEVLFQ↓A-6His-GFP fusion protein (P1' residue Ala)	0.032	0.034	0.91	pH 8, 4 °C	PMID 38521339	Open record: HRV 3C protease, MBP-LEVLFQ↓A-6His-GFP fusion protein (P1' residue Ala)
HRV 3C protease	Human rhinovirus	MBP-LEVLFQ↓C-6His-GFP fusion protein (P1' residue Cys)	0.021	0.045	0.47	pH 8, 4 °C	PMID 38521339	Open record: HRV 3C protease, MBP-LEVLFQ↓C-6His-GFP fusion protein (P1' residue Cys)
HRV 3C protease	Human rhinovirus	MBP-LEVLFQ↓G-6His-GFP fusion protein (P1' residue Gly)	0.22	0.047	4.73	pH 8, 4 °C	PMID 38521339	Open record: HRV 3C protease, MBP-LEVLFQ↓G-6His-GFP fusion protein (P1' residue Gly)
HRV 3C protease	Human rhinovirus	MBP-LEVLFQ↓S-6His-GFP fusion protein (P1' residue Ser)	0.021	0.049	0.39	pH 8, 4 °C	PMID 38521339	Open record: HRV 3C protease, MBP-LEVLFQ↓S-6His-GFP fusion protein (P1' residue Ser)
HRV 3C proteasevariant K22L/F25V/N106Y/T143M/Q145M/T129I	Human rhinovirus	MBP-LEVLFQ↓A-6His-GFP fusion protein (P1' residue Ala)	0.077	0.027	2.84	pH 8, 4 °C	PMID 38521339	Open record: HRV 3C protease variant K22L/F25V/N106Y/T143M/Q145M/T129I, MBP-LEVLFQ↓A-6His-GFP fusion protein (P1' residue Ala)
HRV 3C proteasevariant K22L/F25V/N106Y/T143M/Q145M/T129I	Human rhinovirus	MBP-LEVLFQ↓C-6His-GFP fusion protein (P1' residue Cys)	0.14	0.053	2.65	pH 8, 4 °C	PMID 38521339	Open record: HRV 3C protease variant K22L/F25V/N106Y/T143M/Q145M/T129I, MBP-LEVLFQ↓C-6His-GFP fusion protein (P1' residue Cys)
HRV 3C proteasevariant K22L/F25V/N106Y/T143M/Q145M/T129I	Human rhinovirus	MBP-LEVLFQ↓G-6His-GFP fusion protein (P1' residue Gly)	0.16	0.045	3.62	pH 8, 4 °C	PMID 38521339	Open record: HRV 3C protease variant K22L/F25V/N106Y/T143M/Q145M/T129I, MBP-LEVLFQ↓G-6His-GFP fusion protein (P1' residue Gly)
HRV 3C proteasevariant K22L/F25V/N106Y/T143M/Q145M/T129I	Human rhinovirus	MBP-LEVLFQ↓I-6His-GFP fusion protein (P1' residue Ile)	0.063	0.038	1.71	pH 8, 4 °C	PMID 38521339	Open record: HRV 3C protease variant K22L/F25V/N106Y/T143M/Q145M/T129I, MBP-LEVLFQ↓I-6His-GFP fusion protein (P1' residue Ile)
HRV 3C proteasevariant K22L/F25V/N106Y/T143M/Q145M/T129I	Human rhinovirus	MBP-LEVLFQ↓L-6His-GFP fusion protein (P1' residue Leu)	0.12	0.046	2.65	pH 8, 4 °C	PMID 38521339	Open record: HRV 3C protease variant K22L/F25V/N106Y/T143M/Q145M/T129I, MBP-LEVLFQ↓L-6His-GFP fusion protein (P1' residue Leu)
HRV 3C proteasevariant K22L/F25V/N106Y/T143M/Q145M/T129I	Human rhinovirus	MBP-LEVLFQ↓M-6His-GFP fusion protein (P1' residue Met)	0.18	0.048	3.72	pH 8, 4 °C	PMID 38521339	Open record: HRV 3C protease variant K22L/F25V/N106Y/T143M/Q145M/T129I, MBP-LEVLFQ↓M-6His-GFP fusion protein (P1' residue Met)
HRV 3C proteasevariant K22L/F25V/N106Y/T143M/Q145M/T129I	Human rhinovirus	MBP-LEVLFQ↓N-6His-GFP fusion protein (P1' residue Asn)	0.084	0.08	1.05	pH 8, 4 °C	PMID 38521339	Open record: HRV 3C protease variant K22L/F25V/N106Y/T143M/Q145M/T129I, MBP-LEVLFQ↓N-6His-GFP fusion protein (P1' residue Asn)
HRV 3C proteasevariant K22L/F25V/N106Y/T143M/Q145M/T129I	Human rhinovirus	MBP-LEVLFQ↓Q-6His-GFP fusion protein (P1' residue Gln)	0.033	0.089	0.37	pH 8, 4 °C	PMID 38521339	Open record: HRV 3C protease variant K22L/F25V/N106Y/T143M/Q145M/T129I, MBP-LEVLFQ↓Q-6His-GFP fusion protein (P1' residue Gln)
HRV 3C proteasevariant K22L/F25V/N106Y/T143M/Q145M/T129I	Human rhinovirus	MBP-LEVLFQ↓S-6His-GFP fusion protein (P1' residue Ser)	0.056	0.045	1.45	pH 8, 4 °C	PMID 38521339	Open record: HRV 3C protease variant K22L/F25V/N106Y/T143M/Q145M/T129I, MBP-LEVLFQ↓S-6His-GFP fusion protein (P1' residue Ser)
HRV 3C proteasevariant K22L/F25V/N106Y/T143M/Q145M/T129I	Human rhinovirus	MBP-LEVLFQ↓T-6His-GFP fusion protein (P1' residue Thr)	0.08	0.065	1.23	pH 8, 4 °C	PMID 38521339	Open record: HRV 3C protease variant K22L/F25V/N106Y/T143M/Q145M/T129I, MBP-LEVLFQ↓T-6His-GFP fusion protein (P1' residue Thr)