CatLogenzyme kinetics catalog

EC 3.4.23.3: gastricsin

HydrolasesOpen in Browse

CatLog read 36 records for EC 3.4.23.3 from 2 papers, for 2 organisms. Names in these papers: chymosin, pepsin C.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units.

CatLog also holds 3 records for EC 3.4.23.3 that are not yet re-read: from its earlier version, built from the enzyme databases and partly checked against the papers. All records for EC 3.4.23.3 in Browse.

Papers

PaperOrganismsRecords
Role of S'1 loop residues in the substrate specificities of pepsin A and chymosinCallithrix jacchus24
The catalytic activity of pig pepsin C towards small synthetic substratesSus scrofa12

Records

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)ConditionsPaper
chymosinCallithrix jacchusAla-Phe-Pro-Leu-Glu-Phe-Glu-Arg-Glu-Leu630.304210pH 4, 37 °CPMID 15568804Open
chymosinCallithrix jacchusAla-Phe-Pro-Leu-Glu-Phe-Lys-Arg-Glu-Leu2190.375590pH 4, 37 °CPMID 15568804Open
chymosinCallithrix jacchusLys-Ile-Pro-Tyr-Ile-Leu-Glu-Arg-Gln-Leu460.111420pH 4, 37 °CPMID 15568804Open
chymosinCallithrix jacchusLys-Ile-Pro-Tyr-Ile-Leu-Lys-Arg-Gln-Leu410.059700pH 4, 37 °CPMID 15568804Open
chymosinCallithrix jacchusLys-Ile-Pro-Tyr-Ile-Leu-Phe-Arg-Gln-LeuLeu147-Phe148 bond280.083330pH 4, 37 °CPMID 15568804Open
chymosinCallithrix jacchusLys-Ile-Pro-Tyr-Ile-Leu-Phe-Arg-Gln-LeuPhe148-Arg149 bond (block printed "(F147-R148 cleavage)")1910.1051.8 × 10³pH 4, 37 °CPMID 15568804Open
chymosinvariant D289MCallithrix jacchusAla-Phe-Pro-Leu-Glu-Phe-Glu-Arg-Glu-Leu140.065230pH 4, 37 °CPMID 15568804Open
chymosinvariant D289MCallithrix jacchusAla-Phe-Pro-Leu-Glu-Phe-Lys-Arg-Glu-Leu1010.288350pH 4, 37 °CPMID 15568804Open
chymosinvariant D289MCallithrix jacchusLys-Ile-Pro-Tyr-Ile-Leu-Glu-Arg-Gln-Leu320.054600pH 4, 37 °CPMID 15568804Open
chymosinvariant D289MCallithrix jacchusLys-Ile-Pro-Tyr-Ile-Leu-Lys-Arg-Gln-Leu350.072520pH 4, 37 °CPMID 15568804Open
chymosinvariant D289MCallithrix jacchusLys-Ile-Pro-Tyr-Ile-Leu-Phe-Arg-Gln-LeuLeu147-Phe148 bond—0.054710pH 4, 37 °CPMID 15568804Open
chymosinvariant D289MCallithrix jacchusLys-Ile-Pro-Tyr-Ile-Leu-Phe-Arg-Gln-LeuPhe148-Arg149 bond (block printed "(F147-R148 cleavage)")420.0281.5 × 10³pH 4, 37 °CPMID 15568804Open
chymosinvariant D289M/Q298LCallithrix jacchusAla-Phe-Pro-Leu-Glu-Phe-Glu-Arg-Glu-Leu120.114100pH 4, 37 °CPMID 15568804Open
chymosinvariant D289M/Q298LCallithrix jacchusAla-Phe-Pro-Leu-Glu-Phe-Lys-Arg-Glu-Leu430.25170pH 4, 37 °CPMID 15568804Open
chymosinvariant D289M/Q298LCallithrix jacchusLys-Ile-Pro-Tyr-Ile-Leu-Glu-Arg-Gln-Leu220.046500pH 4, 37 °CPMID 15568804Open
chymosinvariant D289M/Q298LCallithrix jacchusLys-Ile-Pro-Tyr-Ile-Leu-Lys-Arg-Gln-Leu270.087310pH 4, 37 °CPMID 15568804Open
chymosinvariant D289M/Q298LCallithrix jacchusLys-Ile-Pro-Tyr-Ile-Leu-Phe-Arg-Gln-LeuLeu147-Phe148 bond160.0161 × 10³pH 4, 37 °CPMID 15568804Open
chymosinvariant D289M/Q298LCallithrix jacchusLys-Ile-Pro-Tyr-Ile-Leu-Phe-Arg-Gln-LeuPhe148-Arg149 bond (block printed "(F147-R148 cleavage)")170.024700pH 4, 37 °CPMID 15568804Open
chymosinvariant Q298LCallithrix jacchusAla-Phe-Pro-Leu-Glu-Phe-Glu-Arg-Glu-Leu520.463110pH 4, 37 °CPMID 15568804Open
chymosinvariant Q298LCallithrix jacchusAla-Phe-Pro-Leu-Glu-Phe-Lys-Arg-Glu-Leu900.229390pH 4, 37 °CPMID 15568804Open
chymosinvariant Q298LCallithrix jacchusLys-Ile-Pro-Tyr-Ile-Leu-Glu-Arg-Gln-Leu170.062270pH 4, 37 °CPMID 15568804Open
chymosinvariant Q298LCallithrix jacchusLys-Ile-Pro-Tyr-Ile-Leu-Lys-Arg-Gln-Leu300.095320pH 4, 37 °CPMID 15568804Open
chymosinvariant Q298LCallithrix jacchusLys-Ile-Pro-Tyr-Ile-Leu-Phe-Arg-Gln-LeuLeu147-Phe148 bond120.029400pH 4, 37 °CPMID 15568804Open
chymosinvariant Q298LCallithrix jacchusLys-Ile-Pro-Tyr-Ile-Leu-Phe-Arg-Gln-LeuPhe148-Arg149 bond (block printed "(F147-R148 cleavage)")490.0331.5 × 10³pH 4, 37 °CPMID 15568804Open
pepsin CSus scrofaN-acetyl-L-alanyl-L-phenylalanyl-L-leucyl-L-valyl-L-histidinamidekcat/Km column a = ratio of individual kcat and Km7.010.710.1pH 2.1, 37 °CPMID 38772Open
pepsin CSus scrofaN-acetyl-L-alanyl-L-phenylalanyl-L-leucyl-L-valyl-L-histidinamideTable 2 column b——9.11pH 2.1, 37 °CPMID 38772Open
pepsin CSus scrofaN-acetyl-L-phenylalanyl-L-leucyl-L-valyl-L-histidinamidekcat/Km column a = ratio of individual kcat and Km6.121.843.32pH 2.1, 37 °CPMID 38772Open
pepsin CSus scrofaN-acetyl-L-phenylalanyl-L-leucyl-L-valyl-L-histidinamideTable 2 column b——2.86pH 2.1, 37 °CPMID 38772Open
pepsin CSus scrofaN-acetyl-L-phenylalanyl-L-leucyl-L-valyl-L-histidine methyl esterkcat/Km column a = ratio of individual kcat and Km5.131.842.78pH 2.1, 37 °CPMID 38772Open
pepsin CSus scrofaN-acetyl-L-phenylalanyl-L-leucyl-L-valyl-L-histidine methyl esterTable 2 column b——2.15pH 2.1, 37 °CPMID 38772Open
pepsin CSus scrofaN-acetyl-L-tyrosyl-L-leucyl-L-valyl-L-histidinamide[S] range 0.1-2.0 mM4.45——pH 2.1, 37 °CPMID 38772Open
pepsin CSus scrofaN-acetyl-L-tyrosyl-L-leucyl-L-valyl-L-histidinamide[S] range 0.1-0.5 mM1.07——pH 2.1, 37 °CPMID 38772Open
pepsin CSus scrofaN-acetyl-L-tyrosyl-L-leucyl-L-valyl-L-histidinekcat/Km column a = ratio of individual kcat and Km1.080.821.31pH 2.1, 37 °CPMID 38772Open
pepsin CSus scrofaN-acetyl-L-tyrosyl-L-leucyl-L-valyl-L-histidineTable 2 column b——1.07pH 2.1, 37 °CPMID 38772Open
pepsin CSus scrofaN-acetyl-L-tyrosyl-L-leucyl-L-valyl-L-histidine methyl ester[S] range 0.025-1.00 mM5.76——pH 2.1, 37 °CPMID 38772Open
pepsin CSus scrofaN-acetyl-L-tyrosyl-L-leucyl-L-valyl-L-histidine methyl ester[S] range 0.025-0.15 mM1.06——pH 2.1, 37 °CPMID 38772Open