EC 3.4.24.21: astacin
HydrolasesOpen in Browse
CatLog read 40 records for EC 3.4.24.21 from 3 papers, for 2 organisms. Names in these papers: Astacus protease, astacin.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units.
CatLog also holds 6 records for EC 3.4.24.21 that are not yet re-read: from its earlier version, built from the enzyme databases and partly checked against the papers. All records for EC 3.4.24.21 in Browse.
Papers
| Paper | Organisms | Records |
|---|---|---|
| Fluorescent oligopeptide substrates for kinetic characterization of the specificity of Astacus protease | Astacus fluviatilis | 25 |
| A kinetic comparison of the homologous proteases astacin and meprin A | Astacus fluviatilis | 14 |
| Astacin | Astacus astacus | 1 |
Records
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Paper | Open record |
|---|---|---|---|---|---|---|---|---|
| astacin | Astacus astacus | succinylalanylalanylalanylalanylalanyl-4-nitroanilide | 118 | 2.1 | 56.19 | pH 8.25 | PMID 7674929 | Open record: astacin, succinylalanylalanylalanylalanylalanyl-4-nitroanilide |
| astacin | Astacus fluviatilis | 2-aminobenzoyl-arginyl-glycyl-prolyl-phenylalanyl-seryl-prolyl-4-nitrophenylalanyl-arginine | 7.1 | 0.327 | 21.8 | pH 8.7, 30 °C | PMID 8161197 | Open record: astacin, 2-aminobenzoyl-arginyl-glycyl-prolyl-phenylalanyl-seryl-prolyl-4-nitrophenylalanyl-arginine |
| astacin | Astacus fluviatilis | 2-aminobenzoyl-arginyl-hydroxyprolyl-glycyl-phenylalanyl-seryl-prolyl-4-nitrophenylalanyl-arginine | 15.1 | 0.044 | 342 | pH 8.7, 30 °C | PMID 8161197 | Open record: astacin, 2-aminobenzoyl-arginyl-hydroxyprolyl-glycyl-phenylalanyl-seryl-prolyl-4-nitrophenylalanyl-arginine |
| astacin | Astacus fluviatilis | 2-aminobenzoyl-arginyl-prolyl-glycyl-alanyl-seryl-prolyl-4-nitrophenylalanyl-arginine | 44.4 | 0.154 | 288 | pH 8.7, 30 °C | PMID 8161197 | Open record: astacin, 2-aminobenzoyl-arginyl-prolyl-glycyl-alanyl-seryl-prolyl-4-nitrophenylalanyl-arginine |
| astacin | Astacus fluviatilis | 2-aminobenzoyl-arginyl-prolyl-glycyl-glutamyl-seryl-prolyl-4-nitrophenylalanyl-arginine | 2.8 | 0.954 | 2.92 | pH 8.7, 30 °C | PMID 8161197 | Open record: astacin, 2-aminobenzoyl-arginyl-prolyl-glycyl-glutamyl-seryl-prolyl-4-nitrophenylalanyl-arginine |
| astacin | Astacus fluviatilis | 2-aminobenzoyl-arginyl-prolyl-glycyl-leucyl-seryl-prolyl-4-nitrophenylalanyl-arginine | 14.3 | 0.867 | 16.5 | pH 8.7, 30 °C | PMID 8161197 | Open record: astacin, 2-aminobenzoyl-arginyl-prolyl-glycyl-leucyl-seryl-prolyl-4-nitrophenylalanyl-arginine |
| astacin | Astacus fluviatilis | 2-aminobenzoyl-arginyl-prolyl-glycyl-lysyl-seryl-prolyl-4-nitrophenylalanyl-arginine | 3.6 | 0.281 | 12.9 | pH 8.7, 30 °C | PMID 8161197 | Open record: astacin, 2-aminobenzoyl-arginyl-prolyl-glycyl-lysyl-seryl-prolyl-4-nitrophenylalanyl-arginine |
| astacin | Astacus fluviatilis | 2-aminobenzoyl-arginyl-prolyl-isoleucyl-phenylalanyl-seryl-prolyl-4-nitrophenylalanyl-arginine | 78.4 | 0.029 | 2.74 × 10³ | pH 8.7, 30 °C | PMID 8161197 | Open record: astacin, 2-aminobenzoyl-arginyl-prolyl-isoleucyl-phenylalanyl-seryl-prolyl-4-nitrophenylalanyl-arginine |
| astacin | Astacus fluviatilis | arginyl-prolyl-prolyl-glycyl-4-nitrophenylalanyl-alanyl-prolyl-phenylalanyl-arginine | 834 | 0.306 | 2.73 × 10³ | pH 8.7, 30 °C | PMID 8161197 | Open record: astacin, arginyl-prolyl-prolyl-glycyl-4-nitrophenylalanyl-alanyl-prolyl-phenylalanyl-arginine |
| astacin | Astacus fluviatilis | arginyl-prolyl-prolyl-glycyl-4-nitrophenylalanyl-arginyl-prolyl-phenylalanyl-arginine | 0.002 | — | 0.01 | pH 8.7, 30 °C | PMID 8161197 | Open record: astacin, arginyl-prolyl-prolyl-glycyl-4-nitrophenylalanyl-arginyl-prolyl-phenylalanyl-arginine |
| astacin | Astacus fluviatilis | arginyl-prolyl-prolyl-glycyl-4-nitrophenylalanyl-glutamyl-prolyl-phenylalanyl-arginine | 0.001 | — | 0.0014 | pH 8.7, 30 °C | PMID 8161197 | Open record: astacin, arginyl-prolyl-prolyl-glycyl-4-nitrophenylalanyl-glutamyl-prolyl-phenylalanyl-arginine |
| astacin | Astacus fluviatilis | arginyl-prolyl-prolyl-glycyl-4-nitrophenylalanyl-lysyl-prolyl-phenylalanyl-arginine | 0.001 | — | 0.0064 | pH 8.7, 30 °C | PMID 8161197 | Open record: astacin, arginyl-prolyl-prolyl-glycyl-4-nitrophenylalanyl-lysyl-prolyl-phenylalanyl-arginine |
| astacin | Astacus fluviatilis | arginyl-prolyl-prolyl-glycyl-4-nitrophenylalanyl-phenylalanyl-prolyl-phenylalanyl-arginine | 0.004 | — | 0.02 | pH 8.7, 30 °C | PMID 8161197 | Open record: astacin, arginyl-prolyl-prolyl-glycyl-4-nitrophenylalanyl-phenylalanyl-prolyl-phenylalanyl-arginine |
| astacin | Astacus fluviatilis | arginyl-prolyl-prolyl-glycyl-4-nitrophenylalanyl-seryl-prolyl-phenylalanyl-arginine | 78.7 | 0.085 | 922 | pH 8.7, 30 °C | PMID 8161197 | Open record: astacin, arginyl-prolyl-prolyl-glycyl-4-nitrophenylalanyl-seryl-prolyl-phenylalanyl-arginine |
| astacin | Astacus fluviatilis | succinyl-Ala-Ala-Ala-p-nitroanilide | — | — | 0.0217 | pH 8.7, 30 °C | PMID 8161197 | Open record: astacin, succinyl-Ala-Ala-Ala-p-nitroanilide |
| Astacus protease | Astacus fluviatilis | 5-(dimethylamino)naphthalene-1-sulfonyl-Ala-Ala-Arg-Ala-Pro-Leu-Val | 200 | 0.48 | 420 | pH 7.8, 25 °C | PMID 2261483 | Open record: Astacus protease, 5-(dimethylamino)naphthalene-1-sulfonyl-Ala-Ala-Arg-Ala-Pro-Leu-Val |
| Astacus protease | Astacus fluviatilis | 5-(dimethylamino)naphthalene-1-sulfonyl-Arg-Ala-Pro-Leu | 0.035 | 56 | 6 × 10⁻⁴ | pH 7.8 | PMID 2261483 | Open record: Astacus protease, 5-(dimethylamino)naphthalene-1-sulfonyl-Arg-Ala-Pro-Leu |
| Astacus protease | Astacus fluviatilis | 5-(dimethylamino)naphthalene-1-sulfonyl-Gly-Arg-Arg-Ala-Pro-Leu-Gly | 34 | 0.39 | 87 | pH 7.8, 25 °C | PMID 2261483 | Open record: Astacus protease, 5-(dimethylamino)naphthalene-1-sulfonyl-Gly-Arg-Arg-Ala-Pro-Leu-Gly |
| Astacus protease | Astacus fluviatilis | 5-(dimethylamino)naphthalene-1-sulfonyl-Gly-Arg-Arg-Ala-Ser-Leu-Gly | 0.7 | 0.23 | 3 | pH 7.8 | PMID 2261483 | Open record: Astacus protease, 5-(dimethylamino)naphthalene-1-sulfonyl-Gly-Arg-Arg-Ala-Ser-Leu-Gly |
| Astacus protease | Astacus fluviatilis | 5-(dimethylamino)naphthalene-1-sulfonyl-Gly-Gly-Arg-Ala-Pro-Trp-Val | 20 | 5 | 3.9 | pH 7.8 | PMID 2261483 | Open record: Astacus protease, 5-(dimethylamino)naphthalene-1-sulfonyl-Gly-Gly-Arg-Ala-Pro-Trp-Val |
| Astacus protease | Astacus fluviatilis | 5-(dimethylamino)naphthalene-1-sulfonyl-Gly-Lys-Arg-Ala-Pro-Leu-Val | 2.3 | 0.017 | 130 | pH 7.8 | PMID 2261483 | Open record: Astacus protease, 5-(dimethylamino)naphthalene-1-sulfonyl-Gly-Lys-Arg-Ala-Pro-Leu-Val |
| Astacus protease | Astacus fluviatilis | 5-(dimethylamino)naphthalene-1-sulfonyl-Gly-Lys-Arg-Ala-Pro-Leu-Val | 6.53 | 0.06 | 110 calc. | pH 7.8, 25 °C | PMID 2261483 | Open record: Astacus protease, 5-(dimethylamino)naphthalene-1-sulfonyl-Gly-Lys-Arg-Ala-Pro-Leu-Val |
| Astacus protease | Astacus fluviatilis | 5-(dimethylamino)naphthalene-1-sulfonyl-Gly-Lys-Arg-Ala-Pro-Trp-Val | 2.2 | 0.015 | 150 | pH 7.8 | PMID 2261483 | Open record: Astacus protease, 5-(dimethylamino)naphthalene-1-sulfonyl-Gly-Lys-Arg-Ala-Pro-Trp-Val |
| Astacus protease | Astacus fluviatilis | 5-(dimethylamino)naphthalene-1-sulfonyl-Gly-Lys-Asn-Ala-Pro-Leu-Val | 56 | 0.52 | 110 | pH 7.8, 25 °C | PMID 2261483 | Open record: Astacus protease, 5-(dimethylamino)naphthalene-1-sulfonyl-Gly-Lys-Asn-Ala-Pro-Leu-Val |
| Astacus protease | Astacus fluviatilis | 5-(dimethylamino)naphthalene-1-sulfonyl-Gly-Lys-Asn-Ala-Pro-Leu-Val | 39 | 0.4 | 96 | pH 7.8 | PMID 2261483 | Open record: Astacus protease, 5-(dimethylamino)naphthalene-1-sulfonyl-Gly-Lys-Asn-Ala-Pro-Leu-Val |
| Astacus protease | Astacus fluviatilis | 5-(dimethylamino)naphthalene-1-sulfonyl-Gly-Lys-Tyr-Ala-Pro-Trp-Val | 40 | 0.27 | 150 | pH 7.8, 25 °C | PMID 2261483 | Open record: Astacus protease, 5-(dimethylamino)naphthalene-1-sulfonyl-Gly-Lys-Tyr-Ala-Pro-Trp-Val |
| Astacus protease | Astacus fluviatilis | 5-(dimethylamino)naphthalene-1-sulfonyl-Gly-Pro-Arg-Ala-Pro-Leu-Val | 20 | 0.53 | 38 | pH 7.8 | PMID 2261483 | Open record: Astacus protease, 5-(dimethylamino)naphthalene-1-sulfonyl-Gly-Pro-Arg-Ala-Pro-Leu-Val |
| Astacus protease | Astacus fluviatilis | 5-(dimethylamino)naphthalene-1-sulfonyl-His-His-Leu-Lys-Arg-Ala-Pro-Trp-Val | 310 | 0.23 | 1.4 × 10³ | pH 7.8, 25 °C | PMID 2261483 | Open record: Astacus protease, 5-(dimethylamino)naphthalene-1-sulfonyl-His-His-Leu-Lys-Arg-Ala-Pro-Trp-Val |
| Astacus protease | Astacus fluviatilis | 5-(dimethylamino)naphthalene-1-sulfonyl-Leu-Arg-Arg-Ala-Pro-Leu-Gly | 130 | 0.58 | 220 | pH 7.8 | PMID 2261483 | Open record: Astacus protease, 5-(dimethylamino)naphthalene-1-sulfonyl-Leu-Arg-Arg-Ala-Pro-Leu-Gly |
| Astacus protease | Astacus fluviatilis | 5-(dimethylamino)naphthalene-1-sulfonyl-Leu-Arg-Arg-Ala-Ser-Leu-Gly | 2.7 | 0.67 | 4 | pH 7.8 | PMID 2261483 | Open record: Astacus protease, 5-(dimethylamino)naphthalene-1-sulfonyl-Leu-Arg-Arg-Ala-Ser-Leu-Gly |
| Astacus protease | Astacus fluviatilis | 5-(dimethylamino)naphthalene-1-sulfonyl-Leu-Lys-Arg-Ala-Pro-Leu | 43 | 0.87 | 49 | pH 7.8 | PMID 2261483 | Open record: Astacus protease, 5-(dimethylamino)naphthalene-1-sulfonyl-Leu-Lys-Arg-Ala-Pro-Leu |
| Astacus protease | Astacus fluviatilis | 5-(dimethylamino)naphthalene-1-sulfonyl-Leu-Lys-Arg-Ala-Pro-Leu-Val | 120 | 0.25 | 480 | pH 7.8 | PMID 2261483 | Open record: Astacus protease, 5-(dimethylamino)naphthalene-1-sulfonyl-Leu-Lys-Arg-Ala-Pro-Leu-Val |
| Astacus protease | Astacus fluviatilis | 5-(dimethylamino)naphthalene-1-sulfonyl-Leu-Lys-Arg-Ala-Pro-Trp-Val | 210 | 0.33 | 640 | pH 7.8, 25 °C | PMID 2261483 | Open record: Astacus protease, 5-(dimethylamino)naphthalene-1-sulfonyl-Leu-Lys-Arg-Ala-Pro-Trp-Val |
| Astacus protease | Astacus fluviatilis | 5-(dimethylamino)naphthalene-1-sulfonyl-Leu-Lys-Arg-Leu-Pro-Trp-Val | — | — | 0.018 | pH 7.8, 25 °C | PMID 2261483 | Open record: Astacus protease, 5-(dimethylamino)naphthalene-1-sulfonyl-Leu-Lys-Arg-Leu-Pro-Trp-Val |
| Astacus protease | Astacus fluviatilis | 5-(dimethylamino)naphthalene-1-sulfonyl-Leu-Lys-Asn-Ala-Pro-Leu-Val | 180 | 1 | 180 | pH 7.8 | PMID 2261483 | Open record: Astacus protease, 5-(dimethylamino)naphthalene-1-sulfonyl-Leu-Lys-Asn-Ala-Pro-Leu-Val |
| Astacus protease | Astacus fluviatilis | 5-(dimethylamino)naphthalene-1-sulfonyl-Leu-Lys-Lys-Ala-Pro-Trp-Val | 210 | 0.29 | 720 | pH 7.8, 25 °C | PMID 2261483 | Open record: Astacus protease, 5-(dimethylamino)naphthalene-1-sulfonyl-Leu-Lys-Lys-Ala-Pro-Trp-Val |
| Astacus protease | Astacus fluviatilis | 5-(dimethylamino)naphthalene-1-sulfonyl-Leu-Lys-Tyr-Ala-Pro-Trp-Val | 67 | 0.28 | 240 | pH 7.8, 25 °C | PMID 2261483 | Open record: Astacus protease, 5-(dimethylamino)naphthalene-1-sulfonyl-Leu-Lys-Tyr-Ala-Pro-Trp-Val |
| Astacus protease | Astacus fluviatilis | 5-(dimethylamino)naphthalene-1-sulfonyl-Lys-Arg-Ala-Pro-Leu | 40 | 2 | 20 | pH 7.8 | PMID 2261483 | Open record: Astacus protease, 5-(dimethylamino)naphthalene-1-sulfonyl-Lys-Arg-Ala-Pro-Leu |
| Astacus protease | Astacus fluviatilis | 5-(dimethylamino)naphthalene-1-sulfonyl-Pro-Lys-Arg-Ala-Pro-Trp-Val | 380 | 0.37 | 1 × 10³ | pH 7.8, 25 °C | PMID 2261483 | Open record: Astacus protease, 5-(dimethylamino)naphthalene-1-sulfonyl-Pro-Lys-Arg-Ala-Pro-Trp-Val |
| Astacus protease | Astacus fluviatilis | 5-(dimethylamino)naphthalene-1-sulfonyl-Val-Lys-Arg-Ala-Pro-Trp-Val | 190 | 0.25 | 760 | pH 7.8, 25 °C | PMID 2261483 | Open record: Astacus protease, 5-(dimethylamino)naphthalene-1-sulfonyl-Val-Lys-Arg-Ala-Pro-Trp-Val |