Values CatLog read from this paper
Fluorescent oligopeptide substrates for kinetic characterization of the specificity of Astacus protease
PMID 2261483doi:10.1021/bi00497a018Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 25 records (72 values) from this paper: Astacus protease from Astacus fluviatilis.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 1 kcat/Km value marked “calc.” from kcat and Km; no printed kcat/Km was read for that record.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| Astacus protease | Astacus fluviatilis | 5-(dimethylamino)naphthalene-1-sulfonyl-Ala-Ala-Arg-Ala-Pro-Leu-Val | 200 | 0.48 | 420 | pH 7.8, 25 °C | Open record: Astacus protease, 5-(dimethylamino)naphthalene-1-sulfonyl-Ala-Ala-Arg-Ala-Pro-Leu-Val |
| Astacus protease | Astacus fluviatilis | 5-(dimethylamino)naphthalene-1-sulfonyl-Arg-Ala-Pro-Leu | 0.035 | 56 | 6 × 10⁻⁴ | pH 7.8 | Open record: Astacus protease, 5-(dimethylamino)naphthalene-1-sulfonyl-Arg-Ala-Pro-Leu |
| Astacus protease | Astacus fluviatilis | 5-(dimethylamino)naphthalene-1-sulfonyl-Gly-Arg-Arg-Ala-Pro-Leu-Gly | 34 | 0.39 | 87 | pH 7.8, 25 °C | Open record: Astacus protease, 5-(dimethylamino)naphthalene-1-sulfonyl-Gly-Arg-Arg-Ala-Pro-Leu-Gly |
| Astacus protease | Astacus fluviatilis | 5-(dimethylamino)naphthalene-1-sulfonyl-Gly-Arg-Arg-Ala-Ser-Leu-Gly | 0.7 | 0.23 | 3 | pH 7.8 | Open record: Astacus protease, 5-(dimethylamino)naphthalene-1-sulfonyl-Gly-Arg-Arg-Ala-Ser-Leu-Gly |
| Astacus protease | Astacus fluviatilis | 5-(dimethylamino)naphthalene-1-sulfonyl-Gly-Gly-Arg-Ala-Pro-Trp-Val | 20 | 5 | 3.9 | pH 7.8 | Open record: Astacus protease, 5-(dimethylamino)naphthalene-1-sulfonyl-Gly-Gly-Arg-Ala-Pro-Trp-Val |
| Astacus protease | Astacus fluviatilis | 5-(dimethylamino)naphthalene-1-sulfonyl-Gly-Lys-Arg-Ala-Pro-Leu-Val | 2.3 | 0.017 | 130 | pH 7.8 | Open record: Astacus protease, 5-(dimethylamino)naphthalene-1-sulfonyl-Gly-Lys-Arg-Ala-Pro-Leu-Val |
| Astacus protease | Astacus fluviatilis | 5-(dimethylamino)naphthalene-1-sulfonyl-Gly-Lys-Arg-Ala-Pro-Leu-Val | 6.53 | 0.06 | 110 calc. | pH 7.8, 25 °C | Open record: Astacus protease, 5-(dimethylamino)naphthalene-1-sulfonyl-Gly-Lys-Arg-Ala-Pro-Leu-Val |
| Astacus protease | Astacus fluviatilis | 5-(dimethylamino)naphthalene-1-sulfonyl-Gly-Lys-Arg-Ala-Pro-Trp-Val | 2.2 | 0.015 | 150 | pH 7.8 | Open record: Astacus protease, 5-(dimethylamino)naphthalene-1-sulfonyl-Gly-Lys-Arg-Ala-Pro-Trp-Val |
| Astacus protease | Astacus fluviatilis | 5-(dimethylamino)naphthalene-1-sulfonyl-Gly-Lys-Asn-Ala-Pro-Leu-Val | 56 | 0.52 | 110 | pH 7.8, 25 °C | Open record: Astacus protease, 5-(dimethylamino)naphthalene-1-sulfonyl-Gly-Lys-Asn-Ala-Pro-Leu-Val |
| Astacus protease | Astacus fluviatilis | 5-(dimethylamino)naphthalene-1-sulfonyl-Gly-Lys-Asn-Ala-Pro-Leu-Val | 39 | 0.4 | 96 | pH 7.8 | Open record: Astacus protease, 5-(dimethylamino)naphthalene-1-sulfonyl-Gly-Lys-Asn-Ala-Pro-Leu-Val |
| Astacus protease | Astacus fluviatilis | 5-(dimethylamino)naphthalene-1-sulfonyl-Gly-Lys-Tyr-Ala-Pro-Trp-Val | 40 | 0.27 | 150 | pH 7.8, 25 °C | Open record: Astacus protease, 5-(dimethylamino)naphthalene-1-sulfonyl-Gly-Lys-Tyr-Ala-Pro-Trp-Val |
| Astacus protease | Astacus fluviatilis | 5-(dimethylamino)naphthalene-1-sulfonyl-Gly-Pro-Arg-Ala-Pro-Leu-Val | 20 | 0.53 | 38 | pH 7.8 | Open record: Astacus protease, 5-(dimethylamino)naphthalene-1-sulfonyl-Gly-Pro-Arg-Ala-Pro-Leu-Val |
| Astacus protease | Astacus fluviatilis | 5-(dimethylamino)naphthalene-1-sulfonyl-His-His-Leu-Lys-Arg-Ala-Pro-Trp-Val | 310 | 0.23 | 1.4 × 10³ | pH 7.8, 25 °C | Open record: Astacus protease, 5-(dimethylamino)naphthalene-1-sulfonyl-His-His-Leu-Lys-Arg-Ala-Pro-Trp-Val |
| Astacus protease | Astacus fluviatilis | 5-(dimethylamino)naphthalene-1-sulfonyl-Leu-Arg-Arg-Ala-Pro-Leu-Gly | 130 | 0.58 | 220 | pH 7.8 | Open record: Astacus protease, 5-(dimethylamino)naphthalene-1-sulfonyl-Leu-Arg-Arg-Ala-Pro-Leu-Gly |
| Astacus protease | Astacus fluviatilis | 5-(dimethylamino)naphthalene-1-sulfonyl-Leu-Arg-Arg-Ala-Ser-Leu-Gly | 2.7 | 0.67 | 4 | pH 7.8 | Open record: Astacus protease, 5-(dimethylamino)naphthalene-1-sulfonyl-Leu-Arg-Arg-Ala-Ser-Leu-Gly |
| Astacus protease | Astacus fluviatilis | 5-(dimethylamino)naphthalene-1-sulfonyl-Leu-Lys-Arg-Ala-Pro-Leu | 43 | 0.87 | 49 | pH 7.8 | Open record: Astacus protease, 5-(dimethylamino)naphthalene-1-sulfonyl-Leu-Lys-Arg-Ala-Pro-Leu |
| Astacus protease | Astacus fluviatilis | 5-(dimethylamino)naphthalene-1-sulfonyl-Leu-Lys-Arg-Ala-Pro-Leu-Val | 120 | 0.25 | 480 | pH 7.8 | Open record: Astacus protease, 5-(dimethylamino)naphthalene-1-sulfonyl-Leu-Lys-Arg-Ala-Pro-Leu-Val |
| Astacus protease | Astacus fluviatilis | 5-(dimethylamino)naphthalene-1-sulfonyl-Leu-Lys-Arg-Ala-Pro-Trp-Val | 210 | 0.33 | 640 | pH 7.8, 25 °C | Open record: Astacus protease, 5-(dimethylamino)naphthalene-1-sulfonyl-Leu-Lys-Arg-Ala-Pro-Trp-Val |
| Astacus protease | Astacus fluviatilis | 5-(dimethylamino)naphthalene-1-sulfonyl-Leu-Lys-Arg-Leu-Pro-Trp-Val | — | — | 0.018 | pH 7.8, 25 °C | Open record: Astacus protease, 5-(dimethylamino)naphthalene-1-sulfonyl-Leu-Lys-Arg-Leu-Pro-Trp-Val |
| Astacus protease | Astacus fluviatilis | 5-(dimethylamino)naphthalene-1-sulfonyl-Leu-Lys-Asn-Ala-Pro-Leu-Val | 180 | 1 | 180 | pH 7.8 | Open record: Astacus protease, 5-(dimethylamino)naphthalene-1-sulfonyl-Leu-Lys-Asn-Ala-Pro-Leu-Val |
| Astacus protease | Astacus fluviatilis | 5-(dimethylamino)naphthalene-1-sulfonyl-Leu-Lys-Lys-Ala-Pro-Trp-Val | 210 | 0.29 | 720 | pH 7.8, 25 °C | Open record: Astacus protease, 5-(dimethylamino)naphthalene-1-sulfonyl-Leu-Lys-Lys-Ala-Pro-Trp-Val |
| Astacus protease | Astacus fluviatilis | 5-(dimethylamino)naphthalene-1-sulfonyl-Leu-Lys-Tyr-Ala-Pro-Trp-Val | 67 | 0.28 | 240 | pH 7.8, 25 °C | Open record: Astacus protease, 5-(dimethylamino)naphthalene-1-sulfonyl-Leu-Lys-Tyr-Ala-Pro-Trp-Val |
| Astacus protease | Astacus fluviatilis | 5-(dimethylamino)naphthalene-1-sulfonyl-Lys-Arg-Ala-Pro-Leu | 40 | 2 | 20 | pH 7.8 | Open record: Astacus protease, 5-(dimethylamino)naphthalene-1-sulfonyl-Lys-Arg-Ala-Pro-Leu |
| Astacus protease | Astacus fluviatilis | 5-(dimethylamino)naphthalene-1-sulfonyl-Pro-Lys-Arg-Ala-Pro-Trp-Val | 380 | 0.37 | 1 × 10³ | pH 7.8, 25 °C | Open record: Astacus protease, 5-(dimethylamino)naphthalene-1-sulfonyl-Pro-Lys-Arg-Ala-Pro-Trp-Val |
| Astacus protease | Astacus fluviatilis | 5-(dimethylamino)naphthalene-1-sulfonyl-Val-Lys-Arg-Ala-Pro-Trp-Val | 190 | 0.25 | 760 | pH 7.8, 25 °C | Open record: Astacus protease, 5-(dimethylamino)naphthalene-1-sulfonyl-Val-Lys-Arg-Ala-Pro-Trp-Val |