EC 3.4.4.4: trypsin

HydrolasesOpen in Browse

CatLog read 17 records for EC 3.4.4.4 from 1 paper, for 1 organism. Names in these papers: psi-trypsin, alpha-trypsin, beta-trypsin.

EC 3.4.4.4 is no longer current; BRENDA: “transferred to EC 3.4.21.4”.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units.

Papers

Papers with records for EC 3.4.4.4
Paper	Organisms	Records
Comparative kinetic properties of alpha, beta and psi forms of trypsin	Bos taurus	17

Records

Records for EC 3.4.4.4 read from the paper
Enzyme	Organism	Substrate	k_cat (s⁻¹)	K_m (mM)	k_cat/K_m (mM⁻¹ s⁻¹)	Conditions	Paper	Open record
alpha-trypsin	Bos taurus	N2-benzoyl-L-argininamide	0.44	2.3	0.19 calc.	25 °C	PMID 4472038	Open record: alpha-trypsin, N2-benzoyl-L-argininamide
alpha-trypsin	Bos taurus	N2-benzoyl-L-arginine ethyl ester	26.6	0.0026	1 × 10⁴	pH 8, 25 °C	PMID 4472038	Open record: alpha-trypsin, N2-benzoyl-L-arginine ethyl ester
alpha-trypsin	Bos taurus	N2-benzyloxycarbonyl-L-lysine 4-nitrophenyl ester	82	0.01	8.2 × 10³	pH 8, 25 °C	PMID 4472038	Open record: alpha-trypsin, N2-benzyloxycarbonyl-L-lysine 4-nitrophenyl ester
alpha-trypsin	Bos taurus	N2-benzyloxycarbonyl-L-lysine benzyl ester	84	0.08	1 × 10³	pH 8, 25 °C	PMID 4472038	Open record: alpha-trypsin, N2-benzyloxycarbonyl-L-lysine benzyl ester
alpha-trypsin	Bos taurus	N2-benzyloxycarbonyl-L-lysine methyl ester	80	0.125	700	pH 8, 25 °C	PMID 4472038	Open record: alpha-trypsin, N2-benzyloxycarbonyl-L-lysine methyl ester
alpha-trypsin	Bos taurus	N2-p-toluenesulfonyl-L-arginine methyl ester	76	0.017	4.6 × 10³	pH 8, 25 °C	PMID 4472038	Open record: alpha-trypsin, N2-p-toluenesulfonyl-L-arginine methyl ester
beta-trypsin	Bos taurus	N2-benzoyl-L-argininamide	0.69	2.3	0.30 calc.	25 °C	PMID 4472038	Open record: beta-trypsin, N2-benzoyl-L-argininamide
psi-trypsin	Bos taurus	N2-benzoyl-L-arginine ethyl ester	0.24	90	0.0027	pH 8, 25 °C	PMID 4472038	Open record: psi-trypsin, N2-benzoyl-L-arginine ethyl ester
psi-trypsin	Bos taurus	N2-benzyloxycarbonyl-L-lysine 4-nitrophenyl ester	23	1.6	14.4	pH 8, 25 °C	PMID 4472038	Open record: psi-trypsin, N2-benzyloxycarbonyl-L-lysine 4-nitrophenyl ester
psi-trypsin	Bos taurus	N2-benzyloxycarbonyl-L-lysine benzyl esterpsi-trypsin prepared by autolysis of pure alpha-trypsin	8	22	0.36 calc.	pH 8, 25 °C	PMID 4472038	Open record: psi-trypsin, N2-benzyloxycarbonyl-L-lysine benzyl ester
psi-trypsin	Bos taurus	N2-benzyloxycarbonyl-L-lysine benzyl esterTable 1	7.5	22	0.34 calc.	pH 8, 25 °C	PMID 4472038	Open record: psi-trypsin, N2-benzyloxycarbonyl-L-lysine benzyl ester
psi-trypsin	Bos taurus	N2-benzyloxycarbonyl-L-lysine benzyl esterTable 1	7.9	14	0.56 calc.	pH 8, 25 °C	PMID 4472038	Open record: psi-trypsin, N2-benzyloxycarbonyl-L-lysine benzyl ester
psi-trypsin	Bos taurus	N2-benzyloxycarbonyl-L-lysine benzyl esterTable 3 (ester hydrolysis at optimum pH); Table 3	8	22	0.36	pH 8, 25 °C	PMID 4472038	Open record: psi-trypsin, N2-benzyloxycarbonyl-L-lysine benzyl ester
psi-trypsin	Bos taurus	N2-benzyloxycarbonyl-L-lysine methyl ester	0.71	28.5	0.025	pH 8, 25 °C	PMID 4472038	Open record: psi-trypsin, N2-benzyloxycarbonyl-L-lysine methyl ester
psi-trypsin	Bos taurus	N2-p-toluenesulfonyl-L-arginine methyl esterTable 3 (ester hydrolysis at optimum pH); Table 3	1.7	60	0.028	pH 8, 25 °C	PMID 4472038	Open record: psi-trypsin, N2-p-toluenesulfonyl-L-arginine methyl ester
psi-trypsin	Bos taurus	N2-p-toluenesulfonyl-L-arginine methyl esterenzyme 2 µM; Table 4	1.44	59	0.024	pH 8, 25 °C	PMID 4472038	Open record: psi-trypsin, N2-p-toluenesulfonyl-L-arginine methyl ester
psi-trypsin	Bos taurus	N2-p-toluenesulfonyl-L-arginine methyl esterenzyme 2 µM; Table 4	0.6	71	0.0084	pH 8, 25 °C	PMID 4472038	Open record: psi-trypsin, N2-p-toluenesulfonyl-L-arginine methyl ester