Values CatLog read from this paper

Comparative kinetic properties of alpha, beta and psi forms of trypsin

PMID 4472038doi:10.1111/j.1432-1033.1974.tb03693.xOpen in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 17 records (46 values) from this paper: psi-trypsin, alpha-trypsin, beta-trypsin from Bos taurus.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 5 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.

EC 3.4.4.4

Records read from this paper
Enzyme	Organism	Substrate	k_cat (s⁻¹)	K_m (mM)	k_cat/K_m (mM⁻¹ s⁻¹)	Conditions	Open record
alpha-trypsin	Bos taurus	N2-benzoyl-L-argininamide	0.44	2.3	0.19 calc.	25 °C	Open record: alpha-trypsin, N2-benzoyl-L-argininamide
alpha-trypsin	Bos taurus	N2-benzoyl-L-arginine ethyl ester	26.6	0.0026	1 × 10⁴	pH 8, 25 °C	Open record: alpha-trypsin, N2-benzoyl-L-arginine ethyl ester
alpha-trypsin	Bos taurus	N2-benzyloxycarbonyl-L-lysine 4-nitrophenyl ester	82	0.01	8.2 × 10³	pH 8, 25 °C	Open record: alpha-trypsin, N2-benzyloxycarbonyl-L-lysine 4-nitrophenyl ester
alpha-trypsin	Bos taurus	N2-benzyloxycarbonyl-L-lysine benzyl ester	84	0.08	1 × 10³	pH 8, 25 °C	Open record: alpha-trypsin, N2-benzyloxycarbonyl-L-lysine benzyl ester
alpha-trypsin	Bos taurus	N2-benzyloxycarbonyl-L-lysine methyl ester	80	0.125	700	pH 8, 25 °C	Open record: alpha-trypsin, N2-benzyloxycarbonyl-L-lysine methyl ester
alpha-trypsin	Bos taurus	N2-p-toluenesulfonyl-L-arginine methyl ester	76	0.017	4.6 × 10³	pH 8, 25 °C	Open record: alpha-trypsin, N2-p-toluenesulfonyl-L-arginine methyl ester
beta-trypsin	Bos taurus	N2-benzoyl-L-argininamide	0.69	2.3	0.30 calc.	25 °C	Open record: beta-trypsin, N2-benzoyl-L-argininamide
psi-trypsin	Bos taurus	N2-benzoyl-L-arginine ethyl ester	0.24	90	0.0027	pH 8, 25 °C	Open record: psi-trypsin, N2-benzoyl-L-arginine ethyl ester
psi-trypsin	Bos taurus	N2-benzyloxycarbonyl-L-lysine 4-nitrophenyl ester	23	1.6	14.4	pH 8, 25 °C	Open record: psi-trypsin, N2-benzyloxycarbonyl-L-lysine 4-nitrophenyl ester
psi-trypsin	Bos taurus	N2-benzyloxycarbonyl-L-lysine benzyl esterpsi-trypsin prepared by autolysis of pure alpha-trypsin	8	22	0.36 calc.	pH 8, 25 °C	Open record: psi-trypsin, N2-benzyloxycarbonyl-L-lysine benzyl ester
psi-trypsin	Bos taurus	N2-benzyloxycarbonyl-L-lysine benzyl esterTable 1	7.5	22	0.34 calc.	pH 8, 25 °C	Open record: psi-trypsin, N2-benzyloxycarbonyl-L-lysine benzyl ester
psi-trypsin	Bos taurus	N2-benzyloxycarbonyl-L-lysine benzyl esterTable 1	7.9	14	0.56 calc.	pH 8, 25 °C	Open record: psi-trypsin, N2-benzyloxycarbonyl-L-lysine benzyl ester
psi-trypsin	Bos taurus	N2-benzyloxycarbonyl-L-lysine benzyl esterTable 3 (ester hydrolysis at optimum pH); Table 3	8	22	0.36	pH 8, 25 °C	Open record: psi-trypsin, N2-benzyloxycarbonyl-L-lysine benzyl ester
psi-trypsin	Bos taurus	N2-benzyloxycarbonyl-L-lysine methyl ester	0.71	28.5	0.025	pH 8, 25 °C	Open record: psi-trypsin, N2-benzyloxycarbonyl-L-lysine methyl ester
psi-trypsin	Bos taurus	N2-p-toluenesulfonyl-L-arginine methyl esterTable 3 (ester hydrolysis at optimum pH); Table 3	1.7	60	0.028	pH 8, 25 °C	Open record: psi-trypsin, N2-p-toluenesulfonyl-L-arginine methyl ester
psi-trypsin	Bos taurus	N2-p-toluenesulfonyl-L-arginine methyl esterenzyme 2 µM; Table 4	1.44	59	0.024	pH 8, 25 °C	Open record: psi-trypsin, N2-p-toluenesulfonyl-L-arginine methyl ester
psi-trypsin	Bos taurus	N2-p-toluenesulfonyl-L-arginine methyl esterenzyme 2 µM; Table 4	0.6	71	0.0084	pH 8, 25 °C	Open record: psi-trypsin, N2-p-toluenesulfonyl-L-arginine methyl ester