EC 3.5.1.26: N4-(beta-N-acetylglucosaminyl)-L-asparaginase

CatLog read 31 records for EC 3.5.1.26 from 1 paper, for 1 organism. Names in these papers: glycosylasparaginase.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units.

CatLog also holds 19 records for EC 3.5.1.26 that are not yet re-read: from its earlier version, built from the enzyme databases and partly checked against the papers. All records for EC 3.5.1.26 in Browse.

Papers

Papers with records for EC 3.5.1.26
Paper	Organisms	Records
Site-directed mutagenesis of essential residues involved in the mechanism of bacterial glycosylasparaginase	Flavobacterium	31

Records

Records for EC 3.5.1.26 read from the paper
Enzyme	Organism	Substrate	k_cat (s⁻¹)	K_m (mM)	k_cat/K_m (mM⁻¹ s⁻¹)	Conditions	Paper	Open record
glycosylasparaginase	Flavobacterium	asparagine	1.2	0.61	2	pH 7.5, 37 °C	PMID 9545303	Open record: glycosylasparaginase, asparagine
glycosylasparaginase	Flavobacterium	aspartic acid beta-(p-nitroanilide)	2.3	0.43	5.3	pH 7.5, 37 °C	PMID 9545303	Open record: glycosylasparaginase, aspartic acid beta-(p-nitroanilide)
glycosylasparaginase	Flavobacterium	N4-(beta-N-acetyl-D-glucosaminyl)-L-asparagine	16	0.085	190	pH 7.5, 37 °C	PMID 9545303	Open record: glycosylasparaginase, N4-(beta-N-acetyl-D-glucosaminyl)-L-asparagine
glycosylasparaginasevariant D183E	Flavobacterium	N4-(beta-N-acetyl-D-glucosaminyl)-L-asparagine	1	65	0.016	pH 7.5, 37 °C	PMID 9545303	Open record: glycosylasparaginase variant D183E, N4-(beta-N-acetyl-D-glucosaminyl)-L-asparagine
glycosylasparaginasevariant D183N	Flavobacterium	N4-(beta-N-acetyl-D-glucosaminyl)-L-asparagine	0.0095	40	2.4 × 10⁻⁴	pH 7.5, 37 °C	PMID 9545303	Open record: glycosylasparaginase variant D183N, N4-(beta-N-acetyl-D-glucosaminyl)-L-asparagine
glycosylasparaginasevariant D66N	Flavobacterium	asparagine	0.099	0.57	0.17	pH 7.5, 37 °C	PMID 9545303	Open record: glycosylasparaginase variant D66N, asparagine
glycosylasparaginasevariant D66N	Flavobacterium	aspartic acid beta-(p-nitroanilide)	0.44	10	0.043	pH 7.5, 37 °C	PMID 9545303	Open record: glycosylasparaginase variant D66N, aspartic acid beta-(p-nitroanilide)
glycosylasparaginasevariant D66N	Flavobacterium	N4-(beta-N-acetyl-D-glucosaminyl)-L-asparagine	0.27	2.1	0.13	pH 7.5, 37 °C	PMID 9545303	Open record: glycosylasparaginase variant D66N, N4-(beta-N-acetyl-D-glucosaminyl)-L-asparagine
glycosylasparaginasevariant R180K	Flavobacterium	N4-(beta-N-acetyl-D-glucosaminyl)-L-asparagine	0.72	0.7	1	pH 7.5, 37 °C	PMID 9545303	Open record: glycosylasparaginase variant R180K, N4-(beta-N-acetyl-D-glucosaminyl)-L-asparagine
glycosylasparaginasevariant R180L	Flavobacterium	N4-(beta-N-acetyl-D-glucosaminyl)-L-asparagine	1.1	34	0.032	pH 7.5, 37 °C	PMID 9545303	Open record: glycosylasparaginase variant R180L, N4-(beta-N-acetyl-D-glucosaminyl)-L-asparagine
glycosylasparaginasevariant R180Q	Flavobacterium	N4-(beta-N-acetyl-D-glucosaminyl)-L-asparagine	1.3	17	0.076	pH 7.5, 37 °C	PMID 9545303	Open record: glycosylasparaginase variant R180Q, N4-(beta-N-acetyl-D-glucosaminyl)-L-asparagine
glycosylasparaginasevariant T152C	Flavobacterium	N4-(beta-N-acetyl-D-glucosaminyl)-L-asparagine	1.5 × 10⁻⁴	—	—	pH 7.5, 37 °C	PMID 9545303	Open record: glycosylasparaginase variant T152C, N4-(beta-N-acetyl-D-glucosaminyl)-L-asparagine
glycosylasparaginasevariant T152S	Flavobacterium	asparagine	0.026	0.5	0.052	pH 7.5, 37 °C	PMID 9545303	Open record: glycosylasparaginase variant T152S, asparagine
glycosylasparaginasevariant T152S	Flavobacterium	aspartic acid beta-(p-nitroanilide)	0.021	0.34	0.062	pH 7.5, 37 °C	PMID 9545303	Open record: glycosylasparaginase variant T152S, aspartic acid beta-(p-nitroanilide)
glycosylasparaginasevariant T152S	Flavobacterium	N4-(beta-N-acetyl-D-glucosaminyl)-L-asparagine	0.85	0.14	6.1	pH 7.5, 37 °C	PMID 9545303	Open record: glycosylasparaginase variant T152S, N4-(beta-N-acetyl-D-glucosaminyl)-L-asparagine
glycosylasparaginasevariant T170A	Flavobacterium	N4-(beta-N-acetyl-D-glucosaminyl)-L-asparagine	0.0051	—	—	pH 7.5, 37 °C	PMID 9545303	Open record: glycosylasparaginase variant T170A, N4-(beta-N-acetyl-D-glucosaminyl)-L-asparagine
glycosylasparaginasevariant T170C	Flavobacterium	asparagine	0.0022	2.1	0.0011	pH 7.5, 37 °C	PMID 9545303	Open record: glycosylasparaginase variant T170C, asparagine
glycosylasparaginasevariant T170C	Flavobacterium	aspartic acid beta-(p-nitroanilide)	0.0016	0.65	0.0024	pH 7.5, 37 °C	PMID 9545303	Open record: glycosylasparaginase variant T170C, aspartic acid beta-(p-nitroanilide)
glycosylasparaginasevariant T170C	Flavobacterium	N4-(beta-N-acetyl-D-glucosaminyl)-L-asparagine	0.022	0.07	0.31	pH 7.5, 37 °C	PMID 9545303	Open record: glycosylasparaginase variant T170C, N4-(beta-N-acetyl-D-glucosaminyl)-L-asparagine
glycosylasparaginasevariant T170S	Flavobacterium	asparagine	0.23	1.8	0.13	pH 7.5, 37 °C	PMID 9545303	Open record: glycosylasparaginase variant T170S, asparagine
glycosylasparaginasevariant T170S	Flavobacterium	aspartic acid beta-(p-nitroanilide)	0.12	0.32	0.38	pH 7.5, 37 °C	PMID 9545303	Open record: glycosylasparaginase variant T170S, aspartic acid beta-(p-nitroanilide)
glycosylasparaginasevariant T170S	Flavobacterium	N4-(beta-N-acetyl-D-glucosaminyl)-L-asparagine	8	0.078	100	pH 7.5, 37 °C	PMID 9545303	Open record: glycosylasparaginase variant T170S, N4-(beta-N-acetyl-D-glucosaminyl)-L-asparagine
glycosylasparaginasevariant T203A	Flavobacterium	asparagine	0.12	0.6	0.2	pH 7.5, 37 °C	PMID 9545303	Open record: glycosylasparaginase variant T203A, asparagine
glycosylasparaginasevariant T203A	Flavobacterium	aspartic acid beta-(p-nitroanilide)	0.23	0.41	0.56	pH 7.5, 37 °C	PMID 9545303	Open record: glycosylasparaginase variant T203A, aspartic acid beta-(p-nitroanilide)
glycosylasparaginasevariant T203A	Flavobacterium	N4-(beta-N-acetyl-D-glucosaminyl)-L-asparagine	1.5	0.04	39	pH 7.5, 37 °C	PMID 9545303	Open record: glycosylasparaginase variant T203A, N4-(beta-N-acetyl-D-glucosaminyl)-L-asparagine
glycosylasparaginasevariant T203S	Flavobacterium	asparagine	0.81	1.8	0.45	pH 7.5, 37 °C	PMID 9545303	Open record: glycosylasparaginase variant T203S, asparagine
glycosylasparaginasevariant T203S	Flavobacterium	aspartic acid beta-(p-nitroanilide)	1	0.6	1.7	pH 7.5, 37 °C	PMID 9545303	Open record: glycosylasparaginase variant T203S, aspartic acid beta-(p-nitroanilide)
glycosylasparaginasevariant T203S	Flavobacterium	N4-(beta-N-acetyl-D-glucosaminyl)-L-asparagine	3.5	0.076	46	pH 7.5, 37 °C	PMID 9545303	Open record: glycosylasparaginase variant T203S, N4-(beta-N-acetyl-D-glucosaminyl)-L-asparagine
glycosylasparaginasevariant W11S	Flavobacterium	asparagine	0.058	0.19	0.31	pH 7.5, 37 °C	PMID 9545303	Open record: glycosylasparaginase variant W11S, asparagine
glycosylasparaginasevariant W11S	Flavobacterium	aspartic acid beta-(p-nitroanilide)	0.035	0.11	0.32	pH 7.5, 37 °C	PMID 9545303	Open record: glycosylasparaginase variant W11S, aspartic acid beta-(p-nitroanilide)
glycosylasparaginasevariant W11S	Flavobacterium	N4-(beta-N-acetyl-D-glucosaminyl)-L-asparagine	0.034	0.01	3.4	pH 7.5, 37 °C	PMID 9545303	Open record: glycosylasparaginase variant W11S, N4-(beta-N-acetyl-D-glucosaminyl)-L-asparagine