Values CatLog read from this paper

Site-directed mutagenesis of essential residues involved in the mechanism of bacterial glycosylasparaginase

PMID 9545303doi:10.1074/jbc.273.16.9688Open in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 31 records (89 values) from this paper: glycosylasparaginase from Flavobacterium.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.

EC 3.5.1.26

Records read from this paper
Enzyme	Organism	Substrate	k_cat (s⁻¹)	K_m (mM)	k_cat/K_m (mM⁻¹ s⁻¹)	Conditions	Open record
glycosylasparaginase	Flavobacterium	asparagine	1.2	0.61	2	pH 7.5, 37 °C	Open record: glycosylasparaginase, asparagine
glycosylasparaginase	Flavobacterium	aspartic acid beta-(p-nitroanilide)	2.3	0.43	5.3	pH 7.5, 37 °C	Open record: glycosylasparaginase, aspartic acid beta-(p-nitroanilide)
glycosylasparaginase	Flavobacterium	N4-(beta-N-acetyl-D-glucosaminyl)-L-asparagine	16	0.085	190	pH 7.5, 37 °C	Open record: glycosylasparaginase, N4-(beta-N-acetyl-D-glucosaminyl)-L-asparagine
glycosylasparaginasevariant D183E	Flavobacterium	N4-(beta-N-acetyl-D-glucosaminyl)-L-asparagine	1	65	0.016	pH 7.5, 37 °C	Open record: glycosylasparaginase variant D183E, N4-(beta-N-acetyl-D-glucosaminyl)-L-asparagine
glycosylasparaginasevariant D183N	Flavobacterium	N4-(beta-N-acetyl-D-glucosaminyl)-L-asparagine	0.0095	40	2.4 × 10⁻⁴	pH 7.5, 37 °C	Open record: glycosylasparaginase variant D183N, N4-(beta-N-acetyl-D-glucosaminyl)-L-asparagine
glycosylasparaginasevariant D66N	Flavobacterium	asparagine	0.099	0.57	0.17	pH 7.5, 37 °C	Open record: glycosylasparaginase variant D66N, asparagine
glycosylasparaginasevariant D66N	Flavobacterium	aspartic acid beta-(p-nitroanilide)	0.44	10	0.043	pH 7.5, 37 °C	Open record: glycosylasparaginase variant D66N, aspartic acid beta-(p-nitroanilide)
glycosylasparaginasevariant D66N	Flavobacterium	N4-(beta-N-acetyl-D-glucosaminyl)-L-asparagine	0.27	2.1	0.13	pH 7.5, 37 °C	Open record: glycosylasparaginase variant D66N, N4-(beta-N-acetyl-D-glucosaminyl)-L-asparagine
glycosylasparaginasevariant R180K	Flavobacterium	N4-(beta-N-acetyl-D-glucosaminyl)-L-asparagine	0.72	0.7	1	pH 7.5, 37 °C	Open record: glycosylasparaginase variant R180K, N4-(beta-N-acetyl-D-glucosaminyl)-L-asparagine
glycosylasparaginasevariant R180L	Flavobacterium	N4-(beta-N-acetyl-D-glucosaminyl)-L-asparagine	1.1	34	0.032	pH 7.5, 37 °C	Open record: glycosylasparaginase variant R180L, N4-(beta-N-acetyl-D-glucosaminyl)-L-asparagine
glycosylasparaginasevariant R180Q	Flavobacterium	N4-(beta-N-acetyl-D-glucosaminyl)-L-asparagine	1.3	17	0.076	pH 7.5, 37 °C	Open record: glycosylasparaginase variant R180Q, N4-(beta-N-acetyl-D-glucosaminyl)-L-asparagine
glycosylasparaginasevariant T152C	Flavobacterium	N4-(beta-N-acetyl-D-glucosaminyl)-L-asparagine	1.5 × 10⁻⁴	—	—	pH 7.5, 37 °C	Open record: glycosylasparaginase variant T152C, N4-(beta-N-acetyl-D-glucosaminyl)-L-asparagine
glycosylasparaginasevariant T152S	Flavobacterium	asparagine	0.026	0.5	0.052	pH 7.5, 37 °C	Open record: glycosylasparaginase variant T152S, asparagine
glycosylasparaginasevariant T152S	Flavobacterium	aspartic acid beta-(p-nitroanilide)	0.021	0.34	0.062	pH 7.5, 37 °C	Open record: glycosylasparaginase variant T152S, aspartic acid beta-(p-nitroanilide)
glycosylasparaginasevariant T152S	Flavobacterium	N4-(beta-N-acetyl-D-glucosaminyl)-L-asparagine	0.85	0.14	6.1	pH 7.5, 37 °C	Open record: glycosylasparaginase variant T152S, N4-(beta-N-acetyl-D-glucosaminyl)-L-asparagine
glycosylasparaginasevariant T170A	Flavobacterium	N4-(beta-N-acetyl-D-glucosaminyl)-L-asparagine	0.0051	—	—	pH 7.5, 37 °C	Open record: glycosylasparaginase variant T170A, N4-(beta-N-acetyl-D-glucosaminyl)-L-asparagine
glycosylasparaginasevariant T170C	Flavobacterium	asparagine	0.0022	2.1	0.0011	pH 7.5, 37 °C	Open record: glycosylasparaginase variant T170C, asparagine
glycosylasparaginasevariant T170C	Flavobacterium	aspartic acid beta-(p-nitroanilide)	0.0016	0.65	0.0024	pH 7.5, 37 °C	Open record: glycosylasparaginase variant T170C, aspartic acid beta-(p-nitroanilide)
glycosylasparaginasevariant T170C	Flavobacterium	N4-(beta-N-acetyl-D-glucosaminyl)-L-asparagine	0.022	0.07	0.31	pH 7.5, 37 °C	Open record: glycosylasparaginase variant T170C, N4-(beta-N-acetyl-D-glucosaminyl)-L-asparagine
glycosylasparaginasevariant T170S	Flavobacterium	asparagine	0.23	1.8	0.13	pH 7.5, 37 °C	Open record: glycosylasparaginase variant T170S, asparagine
glycosylasparaginasevariant T170S	Flavobacterium	aspartic acid beta-(p-nitroanilide)	0.12	0.32	0.38	pH 7.5, 37 °C	Open record: glycosylasparaginase variant T170S, aspartic acid beta-(p-nitroanilide)
glycosylasparaginasevariant T170S	Flavobacterium	N4-(beta-N-acetyl-D-glucosaminyl)-L-asparagine	8	0.078	100	pH 7.5, 37 °C	Open record: glycosylasparaginase variant T170S, N4-(beta-N-acetyl-D-glucosaminyl)-L-asparagine
glycosylasparaginasevariant T203A	Flavobacterium	asparagine	0.12	0.6	0.2	pH 7.5, 37 °C	Open record: glycosylasparaginase variant T203A, asparagine
glycosylasparaginasevariant T203A	Flavobacterium	aspartic acid beta-(p-nitroanilide)	0.23	0.41	0.56	pH 7.5, 37 °C	Open record: glycosylasparaginase variant T203A, aspartic acid beta-(p-nitroanilide)
glycosylasparaginasevariant T203A	Flavobacterium	N4-(beta-N-acetyl-D-glucosaminyl)-L-asparagine	1.5	0.04	39	pH 7.5, 37 °C	Open record: glycosylasparaginase variant T203A, N4-(beta-N-acetyl-D-glucosaminyl)-L-asparagine
glycosylasparaginasevariant T203S	Flavobacterium	asparagine	0.81	1.8	0.45	pH 7.5, 37 °C	Open record: glycosylasparaginase variant T203S, asparagine
glycosylasparaginasevariant T203S	Flavobacterium	aspartic acid beta-(p-nitroanilide)	1	0.6	1.7	pH 7.5, 37 °C	Open record: glycosylasparaginase variant T203S, aspartic acid beta-(p-nitroanilide)
glycosylasparaginasevariant T203S	Flavobacterium	N4-(beta-N-acetyl-D-glucosaminyl)-L-asparagine	3.5	0.076	46	pH 7.5, 37 °C	Open record: glycosylasparaginase variant T203S, N4-(beta-N-acetyl-D-glucosaminyl)-L-asparagine
glycosylasparaginasevariant W11S	Flavobacterium	asparagine	0.058	0.19	0.31	pH 7.5, 37 °C	Open record: glycosylasparaginase variant W11S, asparagine
glycosylasparaginasevariant W11S	Flavobacterium	aspartic acid beta-(p-nitroanilide)	0.035	0.11	0.32	pH 7.5, 37 °C	Open record: glycosylasparaginase variant W11S, aspartic acid beta-(p-nitroanilide)
glycosylasparaginasevariant W11S	Flavobacterium	N4-(beta-N-acetyl-D-glucosaminyl)-L-asparagine	0.034	0.01	3.4	pH 7.5, 37 °C	Open record: glycosylasparaginase variant W11S, N4-(beta-N-acetyl-D-glucosaminyl)-L-asparagine